Genetic variation in radish (Raphanus sativus L.) germplasm from Pakistan using morphological traits and RAPDs     

Malik Ashiq Rabbani  Yoshie Murakami  Yasuhisa Kuginuki  Kenji Takayanagi 《Genetic Resources and Crop Evolution》1998,45(4):307-316

Genetic diversity of 30 radish (Raphanus sativus L.) accessions was investigated at the phenotypic level with morphological characters and at the DNA level using the random amplified polymorphic DNA (RAPD) technique. Thirty-six morpho-physiological traits were recorded from seedling stage to harvest. The 31 primers used generated 202 RAPD bands, of which 158 (78.2%) were polymorphic. Multivariate procedures were used to classify the germplasm on the basis of phenotypic traits and RAPD fragments. Dendrograms were generated for the Euclidean distance from the morphological data and the Nei's genetic distance from the RAPD markers. Phenotypically, all the accessions were classified into four major groups corresponding to the different forms of cultivated radish. The morphological diversity existing within each of these groups suggested that they should be discriminated into the three botanical convarieties, sativusT (large-rooted), caudatus (pod-type) and oleifer (oilseed-type). Clustering of the accessions did not show any pattern of association between the morphological characters and the collection sites. Instead, landrace groups were associated with their morphological similarities and horticultural uses. On the other hand, the intra-specific genetic relationships of several accessions based on RAPD analysis were related primarily to their collection sites rather than to their phenotypic affinities. The level of polymorphism exhibited by the various convarieties could be exploited in genetic mapping populations to tag economically important traits. These genotypes also could serve as a useful germplasm source for root, leaf, pod and seed. This preliminary study of traditional radish landraces from Pakistan provides useful information regarding their horticultural potential.  相似文献   

Soybean meal can partially replace enzyme-treated fish meal in the diet of juvenile Pacific bluefin tuna <Emphasis Type="Italic">Thunnus orientalis</Emphasis>     

Amal Biswas  Biswajit K. Biswas  Junichi Ito  Osamu Takaoka  Norishige Yagi  Syunsuke Itoh  Kenji Takii 《Fisheries Science》2011,77(4):615-621

This study was carried out to investigate whether expensive enzyme-treated fish meal (EFM) can be partially replaced by soybean meal (SM) or enzyme-treated SM (ESM) without compromising the growth of captive-raised Pacific bluefin tuna (PBT) juveniles. Five fish diets were tested: EFM (control) and EFM in which 10 or 20% of the EFM was replaced with either ESM or SM (ESM₁₀, ESM₂₀, SM₁₀ and SM₂₀, respectively). The respective diets were provided to 28-day-old PBT juveniles (mean body weight approx. 0.38 g) stocked randomly in 15-m³ tanks (276 juveniles per tank; two tanks per treatment) for 12 days. The final mean body weight of PBT juveniles on diets EFM, SM₁₀, and SM₂₀ was significantly higher than that of juveniles on diet ESM₂₀. Although daily feeding rate was significantly higher in the ESM and SM groups, feed efficiency (FE) was significantly higher in the EFM group; there was no significant difference between juveniles fed diets EFM and SM₁₀. Protein and phosphorus retention efficiency were significantly lower in the ESM₂₀, and ESM₂₀ and EFM groups, respectively. Phosphorus discharge was significantly lower in the SM groups. The results demonstrate that 10% SM (11% EFM replacement) can be included in EFM without compromising the growth performance of PBT juveniles.  相似文献   

Improvement of diagnostic markers for resistance to Globodera pallida and application for selection of resistant germplasms in potato breeding     

Kenji Asano  Etsuo Shimosaka  Yoko Yamashita  Takashi Narabu  Satoshi Aiba  Itaru Sakata  Kotaro Akai  Satoshi Okamoto  Seiji Tamiya 《Breeding Science》2021,71(3):354

Occurrence of pale potato cyst nematode, Globodera pallida (Stone) Behrens, was first recorded in Japan in 2015. Among several control measures, cultivation of resistant potato (Solanum tuberosum L.) varieties is the most effective in cost and environmental impact. As no G. pallida-resistant varieties have yet been developed in Japan, great emphasis is being placed on screening of germplasm possessing the resistance and development of the resistant varieties. In this study, we first improved previously reported DNA markers linked to the G. pallida resistance loci (GpaIV^s_adg and Gpa5) and then used these to screen more than 1,000 germplasms to select several candidate germplasms with resistance. We performed inoculation testing on the selected candidates and identified several resistant germplasms to the Japanese G. pallida population. Furthermore, we developed a simultaneous detection method combining three DNA markers linked to G. pallida and Globodera rostochiensis (Wollenweber) Behrens resistance loci. We validated the ability of C237-I marker to select resistant allele of GpaIV^s_adg and predict the presence of resistance in a Japanese breeding population. Resistant germplasms identified in this study could potentially be used to develop G. pallida-resistant varieties. The marker evaluation methods developed in this study will contribute to the efficient development of resistant varieties.  相似文献   

Estimation of kelp forest, <Emphasis Type="Italic">Laminaria</Emphasis> spp., distributions in coastal waters of the Shiretoko Peninsula,Hokkaido, Japan,using echosounder and geostatistical analysis     

Kenji Minami  Hiroki Yasuma  Naoki Tojo  Shin-ichi Fukui  Yusuke Ito  Takahiro Nobetsu  Kazushi Miyashita 《Fisheries Science》2010,76(5):729-736

Sustainable management of the kelp forests of the Shiretoko Peninsula, a World Natural Heritage site, is necessary due to kelp’s ecological and economic importance. The objectives of this study were to estimate the area of kelp forests and to clarify their spatial characteristics in coastal waters of the Shiretoko Peninsula. Data on the presence/absence and thickness of kelp forests were collected via acoustic observation on transects over about 80 km using an echosounder at 200 kHz. Acoustic data were geostatistically interpolated, and the areas covered by kelp forests were estimated. Differences in kelp distribution between the eastern and western sides of the peninsula were compared. The total area of kelp forest was 3.88 km² (eastern area: 3.49 km²; western area: 0.39 km²). The range of thickness of the kelp forests was 34–91 cm. Many kelp forests in the eastern area were thick (>78 cm) and distributed continuously, while kelp forests in the western area were sparsely distributed.  相似文献   

Genetic variability and stock structure of red tilefish Branchiostegus japonicus inferred from mtDNA sequence analysis     

Kenji Nohara  Hiroyuki Takeuchi  Tatsuo Tsuzaki  Nobuaki Suzuki  Osamu Tominaga  Tadahisa Seikai 《Fisheries Science》2010,76(1):75-81

Sequence analysis based on the anterior part of the mitochondrial DNA control region was carried out to reveal the genetic diversity, stock structure, and historical demography of the red tilefish. Nucleotide sequences of 388 bp in length were determined for 280 individuals taken from eight localities. The molecular diversity, haplotype diversity, and nucleotide diversity were relatively high (average h = 0.929 ± 0.011; average π = 0.008 ± 0.005). The mismatch distribution was not significantly different from the expected distribution for a rapidly expanding population (P = 0.453). The minimum spanning network connecting with full-sequence haplotypes contained star-like topologies derived from multiple ancient lineages, supporting the mismatch distribution analysis. No significant genetic differentiation was observed among eight localities (maximum pairwise F _ST = 0.023, reduced-sequence data set). Our results suggest a large panmictic population of the red tilefish along the coast of Honshu to the East China Sea.  相似文献   

Effect of dietary fish oil on enhanced inflammation and disturbed lipophagy in white adipose tissue caused by a high fat diet     

Saitoh  Kanae  Yoshimura  Tomohiro  Sun  Luchuanyang  Yang  Min  Wang  Yao  Taniyama  Shigeto  Hara  Kenji  Murayama  Fumihito  Nikawa  Takeshi  Tachibana  Katsuyasu  Hirasaka  Katsuya 《Fisheries Science》2020,86(1):187-196

Fisheries Science - Fish oil containing omega-3 polyunsaturated fatty acids (PUFA) attenuates chronic inflammation found in obesity, leading to a reduction in insulin resistance. The effect of fish...  相似文献   

Selenoneine suppresses melanin synthesis by inhibiting tyrosinase in murine B16 melanoma cells and 3D-cultured human melanocytes     

Seko  Takuya  Imamura  Shintaro  Ishihara  Kenji  Yamashita  Yumiko  Yamashita  Michiaki 《Fisheries Science》2020,86(1):171-179

Fisheries Science - The novel organic selenium compound, selenoneine, is found in the blood of tuna and has metal-binding activity. In this report, selenoneine displays tyrosinase inhibitory...  相似文献   

Liquid-chromatographic hybrid triple–quadrupole linear-ion-trap MS/MS analysis of fatty-acid esters of dinophysistoxin-1 in bivalves and toxic dinoflagellates in Japan     

Suzuki  Toshiyuki  Kamiyama  Takashi  Okumura  Yutaka  Ishihara  Kenji  Matsushima  Ryoji  Kaneniwa  Masaki 《Fisheries Science》2009,75(4):1039-1048

Fatty-acid esters of dinophysistoxin-1 (DTX1) in scallops Patinopecten yessoensis, mussels Mytilus coruscus, and toxic dinoflagellate Dinophysis species, collected from Japanese seawater, were analyzed by liquid-chromatography mass spectrometry (LC–MS). Precursor ion monitoring, multiple reaction monitoring for 18 fatty-acid esters of DTX1, and full-scan MS/MS spectra obtained with a hybrid triple–quadrupole linear-ion-trap mass spectrometer showed that 14:0, 16:0, and 16:1 esters were the most abundant 7-O-acyl-DTX1 analogues in bivalves. Fatty-acids esters formed by conjugations at hydroxyl positions other than the 7-position of DTX1 were not detected in the bivalves. DTX1 and okadaic acid-16:0 fatty-acid esters have been reported as the most abundant ester in bivalves in several previous studies; however, we found that 7-O-16:1-DTX1 was the most abundant ester in some mussels in which 16:1 was more dominant than 16:0 in the free fatty-acid profile. Comparison between 7-O-acyl-DTX1 and free fatty-acid profiles in the same bivalve samples suggests that polyunsaturated fatty acids are selectively excluded in enzymatic acylation of DTX1. No 7-O-acyl-DTX1 was detected in any single-cell isolates of D. fortii, D. acuminata, D. mitra, D. norvegica, D. tripos, D. infundibulus, and D. rotundata.  相似文献   

Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen     

Inwoo Bae  Kiyoshi Osatomi  Asami Yoshida  Atsuko Yamaguchi  Katsuyasu Tachibana  Tatsuya Oda  Kenji Hara 《Fisheries Science》2009,75(3):765-770

A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological ionic strength and pH in phosphate buffer and then incubating at 25–37°C. During fibril formation from red stingray collagen, the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated by higher pH or lower NaCl concentration. The T _m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution, 33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse stromal cells at 37°C.  相似文献   

Postoperative improvement of megaesophagus and esophageal motility in a 19-day-old puppy with gastroesophageal intussusception     

Ryo TAKEUCHI  Satoshi TAKAGI  Kenji HOSOYA  Hiroshi OHTA  Takaharu ITAMI  Masahiro OKUMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1740

Canine gastroesophageal intussusception (GEI) is a rare and life-threatening condition that requires prompt diagnosis and treatment. A 19-day-old Siberian Husky with a 4-day history of regurgitation was diagnosed with GEI based on the findings of computed tomography (CT) performed without anesthesia. Endoscopic reduction of intussusception was impossible; thus, surgical reduction by traction of the duodenum was performed. CT revealed improvement of megaesophagus 82 days postoperatively. Eleven months postoperatively, fluoroscopy showed recovery to nearly normal esophageal motility. Two years postoperatively, no clinical signs were reported. CT is useful to diagnose GEI in neonate puppies with poor abdominal fat and to assess the gastric edema and the anatomical association of stomach with other organs. Fluoroscopy is helpful for evaluating postoperative esophageal motility.  相似文献