Seasonal parasitism of native egg parasitoids of brown marmorated stink bug (Halyomorpha halys) in Japan     

Kamiyama  Matthew T.  Matsuura  Kenji  Hata  Toshimitsu  Yoshimura  Tsuyoshi  Yang  Chin-Cheng Scotty 《Journal of pest science》2022,95(3):1067-1079

Journal of Pest Science - The invasive, Halyomorpha halys (Hemiptera: Pentatomidae), is a severe economic insect pest native to East Asia. A strong effort has been made to identify natural egg...  相似文献   

Compensatory Cellular Reactions to Nonsteroidal Anti-Inflammatory Drugs on Osteogenic Differentiation in Canine Bone Marrow-Derived Mesenchymal Stem Cells     

Namgil OH  Sangho KIM  Kenji HOSOYA  Masahiro OKUMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):629-636

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Prediction of bending stiffness and moment carrying capacity of sugi cross-laminated timber     

Minoru Okabe  Motoi Yasumura  Kenji Kobayashi  Kazuhiko Fujita 《Journal of Wood Science》2014,60(1):49-58

Cross-laminated timber (CLT) panels consist of several layers of lumber stacked crosswise and glued together on their faces. Prototype sugi CLT floor panels were manufactured and bending tests were carried out under the different parameters of lumber modulus of elasticity (MOE), number of layers, thickness of lumber and thickness of CLT panels. On the basis of above tests, bending stiffness and moment carrying capacity were predicted by Monte Carlo method. MOE of lumber was measured by using grading machine and tensile strength of lumber was assumed to be 60 % of bending strength based on the obtained bending test. Bending stiffness EI of CLT panels could be estimated by adopting composite theory and equivalent section area. Experimental moment carrying capacity showed 12 % higher value than the calculated moment carrying capacity by average lumber failure method, and also showed 45 % higher value than the calculated moment carrying capacity by minimum lumber failure method due to the reinforcement of the outer layer by the neighboring cross layer.  相似文献   

Comparison of the Occurrence of Transportation-associated Fever in 2 Years Old Thoroughbreds before and after Introduction of Prophylactic Marbofloxacin Administration     

Yoshiro ENDO  Takeru TSUCHIYA  Kentaro AKIYAMA  Naoya TAKEBE  Kenji NAKAI  Kenji KOROSUE  Mutsuki ISHIMARU  Nao TSUZUKI  Seiji HOBO 《Journal of Equine Science》2014,25(4):79-81

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Effect of shrimp head protein hydrolysates on the state of water and denaturation of fish myofibrils during dehydration   总被引：1，自引：0，他引：1  

Yaowalux?Ruttanapornvareesakul  Misako?Ikeda  Kenji?HaraEmail author  Kazufumi?Osako  Orawan?Kongpun  Yukinori?Nozaki 《Fisheries Science》2005,71(1):220-228

ABSTRACT:   To utilize fisheries waste products as food materials with functional properties, shrimp head protein hydrolysates (SHPH) from three species of shrimp, that is, Northern pink shrimp ( Pandalus eous ), Endeavour shrimp ( Metapenaeus endeavouri ) and Black tiger shrimp ( Penaeus monodon ), were produced by enzymatic hydrolysis using endopeptidase derived from Bacillus subtilis and exopeptidase derived from Aspergillus oryzae at a level of 0.1% (w/w). SHPH were rich in protein (90–91%) and amino acids (71–84%) but little fat (0.01–0.02%). The average molecular weight of SHPH was 300–1400. The effect of 5% SHPH (dry basis) addition on the state of water and denaturation of lizard fish myofibrils (Mf) during the dehydration process was evaluated by the desorption isotherm and the Ca-ATPase activity, and compared with the effect of sodium glutamate (Na-Glu). SHPH decreased the water activity and the Ca-ATPase inactivation, and increased monolayer sorbed water and multilayer sorbed water of Mf, although these effects of SHPH were smaller than those of Na-Glu. These findings suggest that the SHPH suppressed dehydration-induced denaturation of myofibrillar protein by stabilizing the hydrated water surrounding myofibrils.  相似文献   

Characterization of an Alginate Lyase,FlAlyA, from Flavobacterium sp. Strain UMI-01 and Its Expression in Escherichia coli     

Akira Inoue  Kohei Takadono  Ryuji Nishiyama  Kenji Tajima  Takanori Kobayashi  Takao Ojima 《Marine drugs》2014,12(8):4693-4712

A major alginate lyase, FlAlyA, was purified from the periplasmic fraction of an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01. FlAlyA showed a single band of ~30 kDa on SDS-PAGE and exhibited the optimal temperature and pH at 55 °C and pH 7.7, respectively. Analyses for substrate preference and reaction products indicated that FlAlyA was an endolytic poly(mannuronate) lyase (EC 4.2.2.3). A gene fragment encoding the amino-acid sequence of 288 residues for FlAlyA was amplified by inverse PCR. The N-terminal region of 21 residues except for the initiation Met in the deduced sequence was predicted as the signal peptide and the following region of six residues was regarded as propeptide, while the C-terminal region of 260 residues was regarded as the polysaccharide-lyase-family-7-type catalytic domain. The entire coding region for FlAlyA was subjected to the pCold I—Escherichia coli BL21(DE3) expression system and ~eight times higher yield of recombinant FlAlyA (recFlAlyA) than that of native FlAlyA was achieved. The recFlAlyA recovered in the periplasmic fraction of E. coli had lost the signal peptide region along with the N-terminal 3 residues of propeptide region. This suggested that the signal peptide of FlAlyA could function in part in E. coli.  相似文献   

Photoperiod influences the growth, food intake, feed efficiency and digestibility of red sea bream (Pagrus major)     

Amal K. Biswas  Manabu Seoka  Yuya Inoue  Kenji Takii  Hidemi Kumai 《Aquaculture (Amsterdam, Netherlands)》2005,250(3-4):666-673

Two consecutive trials were conducted to investigate the effects of photoperiod manipulation on growth rate, food intake and feed conversion efficiency (Trial 1), and the digestibility of nutrients and energy (Trial 2) in red sea bream, Pagrus major (body weight 19–120 g). Fish were exposed to four photoperiods (6L:6D, 12L:12D, 16L:8D and 24L:0D) with light intensity 1500 lx on the water surface. The fish were fed with a commercial diet to apparent satiation. In Trial 2, 0.5% chromic oxide (Cr₂O₃) was used as an inert marker. Significantly higher weight gain and specific growth rates were observed in fish exposed to a 24L:0D photoperiod followed by 16L:8D, 6L:6D and 12L:12D photoperiods (P < 0.05). Food intake and feed conversion efficiency (FCE) were also significantly higher in fish exposed to 24L:0D followed by 16L:8D, 6L:6D and 12L:12D photoperiods (P < 0.05). Fish exposed to 24L:0D and 16L:8D photoperiods showed significantly higher lipid and energy digestibility than those exposed to a 12L:12D photoperiod (P < 0.05). The results demonstrated that the enhancement of growth performances under 16L:8D and 24L:0D photoperiods were attributed to improved appetite, greater food intake and higher feed conversion efficiency as well as higher digestibility.  相似文献   

Photoperiod sensitivity gene controlling heading date in rice cultivars in the northernmost region of Japan     

Kenji Fujino 《Euphytica》2003,131(1):97-103

Rice is grown in diverse environments at latitudes ranging from 53^°N to 40^°S. In Japan, Hokkaido is the northernmost rice cultivation region(42–45^°N latitude). Only extremely early maturing (heading) varieties that have extremely low photoperiod sensitivity are adapted to this area. Heading date is the most important trait in adaptation to this particular environment. Also, the efficient manipulation of heading date is a crucial component of rice improvement. To determine the genetic basis for heading date among cultivars grown in Hokkaido, the heading behaviour was analyzed. Clear segregations were observed. To estimate the photoperiod sensitivity of the genes involved, the cultivars and F₁ plants from crosses between the cultivars were grown under different daylength conditions. The results indicated that the genes controlling heading date are photoperiod sensitive, suggesting they play important roles in the northernmost rice cultivation regions in Japan, to which only cultivars with extremely low photoperiod sensitivity are adapted. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Influences of Forest Decline on Various Properties of Soils at Mt. Hirugatake, Tanzawa Mountains, Kanto District, Japan     

Kenji Ohse  Kenji Tamura  Haruo Tanaka  Teruo Higashi 《Soil Science and Plant Nutrition》2005,51(1):37-42

At Mt. Hirugatake in the Tanzawa Mountains, Kanto district, Japan, the deciduous broadleaved forests have rapidly declined. In our previous studies, we reported that the amount of soil organic matter had significantly decreased at the early and final stages of forest decline, and that the soil microbial biomass also showed a large decrease at these stages, suggesting that the composition of soil organic matter might have also changed with forest decline. To clarify the influences of forest decline on the composition of soil organic matter, the amount of humic substances, optical properties of humic acid, and the amount of soil carbohydrates in surface soils at different stages of forest decline were investigated. The amounts of humic acid and fulvic acid decreased to a lesser extent at the early and middle stages of forest decline, and showed a significant decrease at the final stage. As the amount of humin significantly decreased at the early stage, it was plausible that the distinct decrease in the total carbon content of the soil surface horizons at the early stage of forest decline was induced by the decrease in the amount of humin, and at the final stage, by the decrease in the amounts of humic acid and fulvic acid. The amount of soil carbohydrates did not change appreciably with forest decline although the soil organic matter content markedly decreased. It was suggested that most of the carbohydrates in the soil surface horizons were in a stabilized form consisting of complexes with humic substances, metals, and minerals, and would not be affected by the environmental changes associated with forest decline.  相似文献   

Separation of sardine oil without heating from surimi waste and its effect on lipid metabolism in rats     

Toyoshima K  Noguchi R  Hosokawa M  Fukunaga K  Nishiyama T  Takahashi R  Miyashita K 《Journal of agricultural and food chemistry》2004,52(8):2372-2375

Sardine oil was obtained by centrifugation of surimi wastewater without heating or chemical refining. This oil (CE) showed light yellow color and the peroxide value was less than 1.0 meq/kg. The main lipid class of CE was triacylglycerol (TG) (>99%). These features indicate that CE can be directly used as food materials without further purification. Commercial sardine oil (CO) is usually prepared via some kind of refining process with high temperature (250 degrees C) and chemical treatment. The comparative study on the physiological effects of these sardine oils (CE and CO) revealed that the dietary sardine oils were more effective in reducing abdominal fat pads, plasma total cholesterol, and TG levels of rats than was a soybean oil diet (control). Furthermore, these effects were greater in CE than CO, although there was little difference in the fatty acid composition of both oils. Although the main lipid class of CE was TG (>99%), CE was prepared by centrifugation from surimi waste and directly used as dietary fat without further purification. Therefore, CE may contain some kinds of minor components, which could be attributed to the higher physiological activity of CE. To reveal the involvement of the minor compounds in CE, we prepared TG from CE by column chromatography and measured its effect on lipid metabolism of rats. TG from CE also showed the reducing effects on abdominal fad pads and plasma lipid levels. The effect of TG from CE was almost the same as that of original CE, suggesting that the higher nutritional activity of CE than CO may not be due to the minor compounds in CE.  相似文献