Identification of a Germicidal Compound against Picornavirus in Bamboo Pyroligneous Acid     

S Marumoto  SP Yamamoto  H Nishimura  K Onomoto  M Yatagai  K Yazaki  T Fujita  T Watanabe 《Journal of agricultural and food chemistry》2012,60(36):9106-9111

The germicidal activity of pyroligneous acid (PA) against a picornavirus, encephalomyocarditis virus (EMCV), was analyzed, and the component responsible for its disinfectant activity was identified. Bamboo PA (BPA) inactivated EMCV, but neutralization of BPA abolished this activity. Using liquid-liquid phase extraction and silica gel column chromatography, the hydrophobic active fraction of BPA was separated and its 12 major components were identified. The active fraction was reconstructed by mixing synthetic chemicals at the determined concentrations, and a subtraction series of one chemical from the complete mixture was prepared. An in vitro virus assay demonstrated that phenol was the sole germicidal component, and acetic acid augmented the phenol's inactivating activity resulting in >5-log decrease in EMCV infectivity. Considering the low environmental risk of PA, these findings suggest that BPA is a potentially useful agent for preventing viral epidemics in agricultural and human environments.  相似文献   

Proventricular dilatation disease associated with avian bornavirus infection in a Citron-crested Cockatoo that was born and hand-reared in Japan     

Ogawa H  Sanada Y  Sanada N  Kudo M  Tuchiya K  Kodama T  Uetsuka K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(6):837-840

A 5-month-old female Citron-crested Cockatoo (Cacatua sulphurea citrinocristata) that was born and hand-reared in Japan died with suspected proventricular dilatation disease (PDD). Macroscopic and microscopic examinations of the bird revealed characteristic features of PDD, i.e., distention of the proventriculus and infiltration of lymphocytes and plasma cells in ganglia of various organs and in central and peripheral nerves. A linkage of this PDD case to infection with avian bornavirus (ABV) was documented by RT-PCR amplification of the virus genomes from the affected bird. Phylogenetic analysis revealed that the ABV identified in this study clustered into the genotype 2, which is one of the dominant ABV genotypes worldwide. To the best of our knowledge, this is the first report of a natural case of PDD associated with ABV infection in Japan.  相似文献   

Preliminary immunohistochemical study of natriuretic peptide receptor localization in canine and feline heart     

Yamane T  Takemura N  Inoue H  Soeta S  Oishi M  Amasaki H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):375-378

We examined the immunohistochemical distributions of natriuretic peptide receptor (NPR)-A, -B and -C that bind with natriuretic peptide hormones A, B and C in four healthy crossbreed young canine and feline cardiac tissues using specific antibodies against human antigens. Cross-immunoreactivities between antigens and antibodies were confirmed using western blot analysis. NPR-A and -C were expressed more strongly in dogs than cats. In both species, these expressions were stronger in the atria than the ventricles, with stronger expression in the left ventricles than the right. NPR-B was largely very weekly or undetected. In canine and feline cardiac tissues, the expressional distribution of NPR-A, -B, and -C closely matched with that of atrial natriuretic peptide, brain natriuretic peptide, and C-type natriuretic peptide as the ligands for corresponding receptors.  相似文献   

Prevalence of equine herpesvirus type 1 strains of neuropathogenic genotype in a major breeding area of Japan     

Tsujimura K  Oyama T  Katayama Y  Muranaka M  Bannai H  Nemoto M  Yamanaka T  Kondo T  Kato M  Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(12):1663-1667

A single non-synonymous nucleotide substitution of guanine (G) for adenine (A) at position 2254 in the viral DNA polymerase gene (encoded by open reading frame [ORF] 30) of equine herpesvirus type 1 (EHV-1) has been significantly associated with neuropathogenic potential in strains of this virus. To estimate the prevalence of EHV-1 strains with the neuropathogenic genotype (ORF30 G(2254)) in the Hidaka district--a major horse breeding area in Japan--we analyzed the ORF30 genomic region in cases of EHV-1 infection in this area during the years 2001-2010. Of the 113 cases analyzed, 3 (2.7%) were induced by ORF30 G(2254) strains. This prevalence is lower than those observed in the U.S.A. (10.8-19.4%), Argentina (7.4%), France (24%), and Germany (10.6%).  相似文献   

Heterogeneity of circulating autoantibody profiles in canine autoimmune subepidermal blistering dermatoses     

Nishifuji K 《Veterinary dermatology》2011,22(1):118-118

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Effects of transforming growth factor-β3 and matrix metalloproteinase-3 on the pathogenesis of chronic mitral valvular disease in dogs     

Obayashi K  Miyagawa-Tomita S  Matsumoto H  Koyama H  Nakanishi T  Hirose H 《American journal of veterinary research》2011,72(2):194-202

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Effects of oocyte‐derived paracrine factors on release of extracellular vesicles by murine mural granulosa cells in vitro     

Yuta Matsuno  Natsumi Maruyama  Wataru Fujii  Kunihiko Naito  Koji Sugiura 《Animal Science Journal》2020,91(1)

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Supplementation with eicosapentaenoic acid and linoleic acid increases the production of epidermal ceramides in in vitro canine keratinocytes     

Ji‐Seon Yoon  Koji Nishifuji  Toshiro Iwasaki 《Veterinary dermatology》2020,31(5):419-e112

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Effects of hydroxy-aluminum on flocculation and permeability of silt and clay fractions separated from paddy soils     

Koji Wada  Yasutaka Tsumori  Yoshikazu Nitawaki  Kazuhiko Egashira 《Soil Science and Plant Nutrition》2013,59(3):313-322

Flocculation tests and permeability measurements were carried out for silt and clay fractions separated from surface and subsurface horizons of four paddy soils. There was not much difference in the flocculating power between A1(OH)_2.2C1_0.8 and CaSO₄. Their flocculation value, 0.3 to 3 mM, was attained by adding the reagents at the rate of 0.24 to 2.4 mmol/100 g of the silt and clay. The flocculation with A1(OH)_2.2C1_0.8 or CaSO₄ did not affect the sediment volume of the silt and clay. This sediment volume was smaller for the surface than for the subsurface horizons of each soil, suggesting that soil organic matter and repetition of wetting and drying contribute to the stable aggregation of the silt and clay in the surface horizon. The permeability was higher for the silt and clay fractions separated from the surface horizon than that from the subsurface horizon, and the addition of 2.5 to 4.2 mmol of A1(OH)_2.2C1_0.8 or CaSO₄/100g of the silt and clay increased its permeability by 1.5 to 4 times. The effect of A1(OH)_2.2C1_0.8 was more lasting than that of CaSO₄ and was recognized even after 200 ml of water passed through 1 g of the silt and clay. The effect of the flocculating reagent on the permeability was more or less enhanced by air-drying the treated silt and clay.  相似文献   

Adsorption of alkali chloride and ammonium halide on halloysite     

Koji Wada 《Soil Science and Plant Nutrition》2013,59(3):137-144

It is generally accepted that broken bonds are the major cause of cation exchange in kaolin minerals, although the studies of a “fireclay” type of kaolin mineral (8) and hydrated halloysite (12, 15) seem to indicate that lattice substitutions, such as that found for 2 : 1 type of clay minerals, account for the fairly large parts of the cation-exchange capacities of some kaolin clays, particularly with poor crystallinity.  相似文献