Potential of seed and root exudates of the common bean Phaseolus vulgaris L. for immediate induction of rhizobial chemotaxis and nod genes     

Kotaro KATO  Yasuhiro ARIMA 《Soil Science and Plant Nutrition》2006,52(4):432-437

  相似文献   

Molecular analyses of a potentially novel Anaplasma species closely related to Anaplasma phagocytophilum detected in sika deer (Cervus nippon yesoensis) in Japan     

Ybañez AP  Matsumoto K  Kishimoto T  Inokuma H 《Veterinary microbiology》2012,157(1-2):232-236

An Anaplasma species closely related to Anaplasma phagocytophilum detected in sika deer in Hokkaido, Japan was molecularly analyzed using 16S rRNA, citrate synthase (gltA), and heat-shock operon (groEL) gene sequences. Genome walking was performed to determine its complete gltA and groEL sequences (1233 bp and 1650 bp, respectively). Percent identities to the closest A. phagocytophilum sequences from the US and European strains were 98.6-98.8%, 76.5%, and 80.3-80.8% for 16S rRNA, gltA, and groEL genes, respectively. For deduced amino acid sequences, percent identities to the closest A. phagocytophilum sequences were 66.7% and 97.6% for gltA and groEL genes, respectively. Phylogenetic analyses revealed divergence from any known A. phagocytophilum strain. The lower identities and the divergent phylogenetic position of the Anaplasma sp. detected from sika deer in Japan with established A. phagocytophilum strains provide evidence of its potential novelty.  相似文献   

Serological evidence of infection of Anaplasma and Ehrlichia in domestic animals in Xinjiang Uygur Autonomous Region area, China     

Chahan B  Jian Z  Xuan X  Sato Y  Kabeya H  Tuchiya K  Itamoto K  Okuda M  Mikami T  Maruyama S  Inokuma H 《Veterinary parasitology》2005,134(3-4):273-278

Serological methods were utilized to detect Anaplasma and Ehrlichia infection in domestic animals in Xinjiang Uygur Autonomous Region, China. By using an indirect immunofluorescence assay (IFA), antibodies that reacted with Anaplasmaphagocytophilum and Ehrlichiachaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were recorded. These results indicate that ruminants kept in these areas may be infected with some species of Anaplasma and Ehrlichia.  相似文献   

Expression in Escherichia coli and purification of the functional feline granulocyte colony-stimulating factor     

Yamamoto A  Iwata A  Saitoh T  Tuchiya K  Kanai T  Tsujimoto H  Hasegawa A  Ishihama A  Ueda S 《Veterinary immunology and immunopathology》2002,90(3-4):169-177

Feline granulocyte colony-stimulating factor (G-CSF) with an N-terminal histidine hexamer tag was expressed as inclusion bodies in E. coli. The G-CSF solubilized in 6 M guanidine solution was absorbed onto a Ni-NTA column and, after washing with decreasing concentrations of guanidine, eluted with imidazole in a soluble and apparently pure form. The activity of the recombinant feline G-CSF was 3×10⁶ U/mg protein, as assayed by its stimulatory effect on NFS-60 cell proliferation. When a low level of purified feline G-CSF was administered once a day for two successive days to cats, the number of neutrophil increased 4-fold while the levels of other blood cell types remained virtually unchanged. Daily administration of G-CSF for a total of 11 days led to a more than 10-fold increase in neutrophils, an 8-fold increase in the number of monocytes and 2-fold increase in lymphocytes. No severe side effects or antibody production was observed in cats after administration of G-CSF.  相似文献   

Identification of ovalbumin phenotypes of the Asian indigenous chicken populations using polymerase chain reaction-restriction fragment length polymorphism     

Keiji KINOSHITA  Takeshi SHIMOGIRI  Shin OKAMOTO  Kotaro KAWABE  Joji ISHIZUKA  Hisham R. IBRAHIM  Yoshizane MAEDA 《Animal Science Journal》2003,74(5):355-362

Three electrophoretic variations (AA, BB and AB) of ovalbumin controlled by codominant alleles Ov^A and Ov^B have been observed in various chicken populations. We compared nucleotide sequences of the open reading frame between two alleles of ovalbumin gene. The difference between the two alleles was found as a non‐synonymous substitution of asparagine to aspartic acid as a result of AAT to GAT point mutation at position 8032–8034 in exon 8. We developed polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) protocol in combination with Mbo I restriction endnuclease mapping for the detection of this substitution. By the PCR‐RFLP the allelic frequency of the Ov^B was estimated to be within the range of 0.000–0.150 in 11 Asian indigenous chicken populations and 0.000 in four improved breeds used in the present study. Gene frequency, estimated by PCR‐RFLP in the present study, paralleled that obtained by protein polymorphisms of egg white. Thus, this study provides, for the first time, information of the occurrence of ovalbumin allele Ov^A and Ov^B in Asian indigenous chicken populations.  相似文献   

Prevalence of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in piglets after weaning on a commercial pig farm in Japan     

Kenichi SASAKI  Takamitsu TSUKAHARA  Osamu TAIRA  Kotaro TSUCHIYA  Mitsugi ITOH  Kazunari USHIDA 《Animal Science Journal》2010,81(1):135-141

To investigate the transition in concentration of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) and antibody for these viruses in serum, serum samples were collected from 29 pigs on weaning day and at 7, 14, 21, 28, 53, 84, and 120 days after weaning. The concentration of circulated PRRSV and PCV2 in serum was measured by real-time RT-PCR and real-time PCR, respectively. The specific antibody for PRRSV and PCV2 was measured using ELISA. PRRSV was not detected on 0 days post-weaning (dpw). The specific antibody for PRRSV began to increase as the concentration of PRRSV in serum increased, and the level of PRRSV then tended to decrease. PCV2 was detected in 12 of 28 pigs on 0 dpw. The concentration of PCV2 and the specific antibody for PCV2 showed a similar tendency to those of PRRSV. The correlation analysis suggests that a decline in the daily weight gain coincided with an increase in the PRRSV concentration. Pigs with a higher antibody titer against PRRSV or PCV2 on 0 dpw showed the lower level of PRRSV or PCV2, respectively.  相似文献   

Assessing the usefulness of otolith elemental compositions for evaluating the population structure of the Pacific herring <Emphasis Type="Italic">Clupea pallasii</Emphasis> in northern Japan     

Kodai Yamane  Kotaro Shirai  Yoshitomo Nagakura  Tsuguo Otake 《Fisheries Science》2012,78(2):295-307

The present study examined whether the elemental compositions of whole otoliths and chronological transect profiles of otoliths covering the complete life history are useful for evaluating the population structure of Pacific herring. Eight elemental ratios, Li:Ca, Na:Ca, Mg:Ca, K:Ca, Mn:Ca, Cu:Ca, Sr:Ca, and Ba:Ca, were measured in whole otoliths and in a series of ablations across the life-history transects by laser ablation inductively coupled plasma mass spectrometry. We studied fish from three sample groups collected from Lake Obuchinuma, Miyako Bay, and Akkeshi Bay of northern Japan from March to May 2008. Significant differences were found in the Na:Ca, Mg:Ca, P:Ca, K:Ca, Mn:Ca, and Ba:Ca ratios of the whole otoliths and in the Li:Ca, Na:Ca, K:Ca, Mn:Ca, Sr:Ca, and Ba:Ca ratios of the otolith core among the three sample groups. Therefore, the present study showed clear differences in elemental composition among the three sample groups from different locations, suggesting that otolith elemental compositions reflect the habitats experienced by individual fish.  相似文献   

Small and round seed 5 gene encodes alpha-tubulin regulating seed cell elongation in rice   总被引：1，自引：0，他引：1  

Shuhei Segami  Izumi Kono  Tsuyu Ando  Masahiro Yano  Hidemi Kitano  Kotaro Miura  Yukimoto Iwasaki 《Rice》2012,5(1):4

Seed size is an important trait in determinant of rice seed quality and yield. In this study, we report a novel semi-dominant mutant Small and round seed 5 (Srs5) that encodes alpha-tubulin protein. Lemma cell length was reduced in Srs5 compared with that of the wild-type. Mutants defective in the G-protein alpha subunit (d1-1) and brassinosteroid receptor, BRI1 (d61-2) also exhibited short seed phenotypes, the former due to impaired cell numbers and the latter due to impaired cell length. Seeds of the double mutant of Srs5 and d61-2 were smaller than those of Srs5 or d61-2. Furthermore, SRS5 and BRI1 genes were highly expressed in Srs5 and d61-2 mutants. These data indicate that SRS5 independently regulates cell elongation of the brassinosteroid signal transduction pathway  相似文献   

Informing CITES Parties: Strengthening science‐based decision‐making when listing marine species     

Kim Friedman  Matias Braccini  Matthew Bjerregaard‐Walsh  Ramn Bonfil  Corey J. A. Bradshaw  Stephen Brouwer  Ian Campbell  Rui Coelho  Enric Corts  Wetjens Dimmlich  Michael G. Frisk  Irene Kingma  Sophy R. McCully Phillips  Colman O'Criodain  Denham Parker  Samuel Shephard  Javier Tovar‐vila  Kotaro Yokawa 《Fish and Fisheries》2020,21(1):13-31

International trade in vulnerable marine species is regulated once they are listed in CITES Appendices (the Convention on International Trade in Endangered Species of Wild Fauna and Flora). Parties to the Convention submit proposal(s) 150 days prior to the CITES Conference for voting on the inclusion of new species in Appendices I and II, making a case for why CITES listing criteria are met in each case. Before the vote, Parties receive advice from (a) the Food and Agriculture Organization of the United Nations, (b) the International Union for Conservation of Nature—TRAFFIC and (c) the CITES Secretariat, among others. This paper offers an expert review of listing processes, which are the subject of much debate in fishery and environment‐protection communities, looking at two specific cases: silky shark (Carcharhinus falciformis, Carcharhinidae) and bigeye thresher shark (Alopias superciliosus, Alopiidae). The reviewers determine that the evidence made available to voting Parties is substantial, but suffers from non‐standard presentation across assessments. The best available data are not always presented or described transparently in relation to CITES criteria. An extension of the assessment period, as well as the opportunity to refute evidence, has been suggested as ways to support more informed and effective decision‐making by CITES Parties, whose composition of delegations varies greatly in their experience of marine species management and trade. Experts welcomed a greater coherence of advice between fishery and non‐fishery sources in the long term, and proposed a range of suggested improvements for the delivery of information and advice to CITES Parties.  相似文献   

Reproductive biology of blue marlin <Emphasis Type="Italic">Makaira nigricans</Emphasis> around Yonaguni Island,southwestern Japan     

Tamaki Shimose  Maki Fujita  Kotaro Yokawa  Hirokazu Saito  Katsunori Tachihara 《Fisheries Science》2009,75(1):109-119

We studied the spawning seasonality and gonadal development of blue marlin Makaira nigricans using specimens captured around Yonaguni Island in southwestern Japan between February 2003 and February 2006. The mean (±SD) lower jaw–fork length of females (234 ± 24 cm) was greater than that of males (191 ± 12 cm). The smallest mature female and male were 183 and 160 cm, respectively. Most of the 717 females had immature ovaries. However, in March and from May to September, the ovaries of 26 females contained oocytes with yolk globules, hydrated oocytes, or postovulatory follicles. Most males had testes with a large amount of spermatozoa throughout the year. The occurrence of mature blue marlin at Yonaguni Island suggests that spawning occurs here. The mean condition factors (fatness of the fish) of both sexes decreased from March to June or July, presumably as they expended energy to reproduce. We discuss our results in the context of migration theory for blue marlin in the western North Pacific Ocean.  相似文献