昆虫病原线虫共生细菌胞内晶体蛋白的研究进展     

游娟梁世中  韩日畴 《昆虫天敌》2005,27(2):76-82

初生型发光杆菌属（Photorhabdus）及嗜线虫致病杆菌属（Xenorhabdus）细菌分别与异小杆线虫属（Heterorhabdltis）和斯氏线虫属（Steinernema）昆虫病原线虫互惠共生。这类昆虫病原细菌在稳定生长期分别产生两种形态各异的胞内晶体蛋白。本文回顾了这类蛋白的研究历史和最新的研究进展，特别是胞内晶体蛋白的理化性质和生物学功能，同时讨论这种晶体蛋白的研究方法与技术。  相似文献   

斯氏线虫在进境原木害虫检疫处理上的应用前景     

胡学难吴佳教  荣晓东莫仁浩 梁帆 《昆虫天敌》2005,27(4):178-182

本文综述了我国进境原木上截获的主要害虫类群；总结了目前进境原木害虫的检疫处理技术；重点介绍了斯氏线虫Steinernema sp．对钻蛀性害虫的防治研究，探讨了斯氏线虫在进境原木害虫检疫处理上的应用前景，提出了应用斯氏线虫防治进境原木害虫所存在的问题及今后的研究方向。  相似文献   

提高养鸡生产经济效益的技术措施     

梁琛  张建海 《水禽世界》2005,(9):20-21

养鸡户缺乏科学的饲养管理技术，饲养工艺设计不合理，饲料营养浪费，重治不重防，滥用药物，导致治疗效果不佳，死淘率升高，甚至发生中毒等。已成为我国养鸡生产中较为普遍的问题，加上市场波动，直接影响养鸡业的经济效益。严重挫伤了养殖户的积极性。尤其是广大农村，水平低，投入高，效益差的“穷养鸡”方式一度存在。因此。科学的管理，最大限度降低养殖成本，是提高养鸡生产经济效益、确保养鸡业健康发展的重要举措。  相似文献   

Decreased fibroblast proliferation and collagen content in the wound of diabetic rats caused by streptozotocin     

LI Ping  LI Guang-shan  SHENG Xun  WANG Fang  LIANG Dai-ying  LIU Xin  HUANG Qi-fu 《园艺学报》2005,21(9):1807-1810

AIM: To study fibroblast proliferation and collagen synthesis during wound healing in diabetic rats induced by streptozotocin. METHODS: 30 Wistar male rats were randomly divided into control group and model group. 55 mg/kg STZ were given intraperitoneally to model rats. After 3 weeks, a round skin of 2.04 cm2 was excised on all dorsal back of rats. The healing time and healing rate were observed according to re-epithelization. The numbers of fibroblasts and the expression of proliferating cell nuclear antigen (PCNA) were observed by Hematoxylin-Eosin (HE) staining and immuno-histochemistry assay. Collagen Ⅰ and Ⅲ stained by Picric acid-Sirius red were calculated by image analysis. RESULTS: The healing time in model group was (27.13±1.81) days，significantly longer than that in control group ［(15.25±1.67） days， P<0.01］. The healing rates in model group were significantly less than that in control group at day 3, day 7 and day 15 (P<0.01). The amount of fibroblasts and the expression of PCNA in model group were significantly less than those in control group on day 3, day 5, day 7 and day 9, respectively (P<0.05, P<0.01). Even the content of collagen I in the wound of both groups increased with time, the values were much higher than that in model group at different times (P<0.05), respectively. For model group, the ratio of collagen Ⅰ/Ⅲ was less than that in control group 3, 7 and 11 days after wound (P<0.01). CONCLUSION: STZ impaires wound healing in rats, which is possible caused by the disturbance of fibroblast proliferation and collagen synthesis in the wound.  相似文献   

节瓜蓟马的新天敌——中华微刺盲蝽   总被引：8，自引：2，他引：8  

秦玉洁  吴伟坚等 《昆虫天敌》2001,23(3):115-118

首次发现中华微刺盲蝽Campylomma chinensis Schwh捕食节瓜蓟马Thrips palmi Karny且对田间节瓜蓟马种群数量的减少起重要作用。本文研究了该虫的形态特征、捕食过程和取食范围。结果表明中华微刺盲蝽为多食性昆虫，能取食多种小型昆虫及多种昆虫的卵：在田间，中华微刺盲蝽与节瓜蓟马数量的跟随现象明显；通过释入盲蝽，能使节瓜蓟马种群数量减少。  相似文献   

苏云金素在水中和植株上的衰减与残留     

林开春  操继跃 《中国生物防治》1995,11(3):97-100

用ＨＰＬＣ检测了苏云金素在鱼塘水和清水中的衰减速率。清水中该物质的半衰期长达４３３小时；在鱼塘水中苏云素的衰减呈双指数消除模型，开始消除半衰期为２．４３－１０．６８小时，终末消除半衰期为１２８．５７－１４２．０１小时。  相似文献   

A Review on How Plant Hormones and Environment Factors Are Involved in Rice Root Hair Development     

LI Bin  HUANG Jin  WANG Li  LI Jin  LIANG Yueyang  CHEN Ji 《中国水稻科学》1986,34(4):287-299

Plant root hairs are important structures on the root surface for absorption of water, inorganic and organic nutrients. The root hairs which are regulated by both genetic and environmental factors, also play important roles when plants are dealing with environmental stress conditions. In this review, we analyzed how stress such as P deficiency, genetic factors and the core regulatory network composed of ABA，ETH, ABA work together during the regulation of rice root hair development. At last, we come up with issues which remains unclear but are critical and could be potential research focus.  相似文献   

Experimental Pathological Study of Acute African Swine Fever     

DENG Hua  LI Hui  YANG Hong  ZHOU Zhaohai  LIANG Haozhao  XU Zhigao  WU Fuda  LI Qiaofeng  HUANG Luqi 《畜牧兽医学报》1956,51(11):2836-2848

To study the clinicopathology and histopathology of African swine fever (ASF), and to explore the internal relationship between pathological changes and disease occurrence and development and its pathological mechanism, 13 Landrace pigs with bodyweight about 20 kg were intramuscular injected with African swine fever virus (ASFV), strain Pig/HLJ/18 at a dose of 10²HAD₅₀·mL^-1. During the experiment, all the dead pigs were systematically dissected and sampled, paraffin sections were produced, and haematoxylin-eosin staining was performed. Clinicopathological evaluation standards for acute ASF were established, then pathological lesions (classification variables) were expressed by counting frequency and percentage, and the lesion degree (continuous variables) was graded and scored according to different pathological changes of various tissues and organs. The results showed that all infected pigs were in line with the clinical characteristics of ASF, including acute, febrile and highly infectious, with a 100% incidence rate and 100% mortality. The dead pigs showed typical characteristics of septicemia, cadavers prone to corruption, blood clotting adverse or hemolysis, rigor mortis incomplete. The main pathological lesions were hemorrhagic necrotizing lymphadenitis, acute inflammatory splenomegaly (septic spleen), cerebral edema, pulmonary edema and lung consolidation et al. The spleen and lymphonodus are the target organs attacked by ASFV, with the most significant lesions, the earliest occurrence time, the longest duration and the highest frequency. The most prominent pathological changes are blood circulation disorders, including multiple pathological manifestations such as edema, hyperemia, congestion, hemorrhage, infarction, disseminated intravascular coagulation (DIC), and the most important characteristics are hemorrhagic lesions. The inflammatory reaction of lymphocytic exudation caused by ASFV runs through the whole process, especially in the middle and later stages of the course. The results suggest that the main pathological process of acute African swine fever is a typical immune/inflammatory cascade reaction and severe systemic blood circulation disorder, which resulted in the high incidence rate and high mortality rate of acute ASF.  相似文献   

Non-steroidal anti-inflammatory drugs induce apoptosis in implanted hepatoma of mice     

WU Wei-jiang  LI Xiao-hong  REN Xian-da  LUO Yu-hong  ZHOU Qi-jing  LIANG Xiao-yu 《园艺学报》2003,19(8):1074-1078

AIM: To study the mechanism of the effect of NSAIDs on apoptosis in mice hepatoma at anti-inflammatory doses. METHODS: Kunming breed mice were inoculated subcutaneously in the flank with mice hepatoma H22 cell line. The effects of ibuprofen, indomethacin, and nimesulide on apoptosis were determined by using electron microscopy, agarose gel electrophoresis, flow cytometry, and Western blot analysis of the expression of c-myc, bcl-x_L and bcl-2 proteins. RESULTS: NSAIDs induced apoptosis of mice implanted hepatoma, which includes the morphological changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the "ladder pattern" revealed by agarose gel electrophoresis of DNA. The apoptotic index was increased to 15%±1.0%, 29.7%±1.5%, 46.3%±3.5% from 3.3%±0.6% by detecting Sub-G₁ peaks on flow cytometry. Western blot analysis showed that levels of bcl-2 and bcl-x_L were significantly reduced by treatment with nimesulide. Ibuprofen and indomethacin decreased bcl-2 expression but increased bcl-x_L expression. C-myc wasn't changed in these groups. CONCLUSION: These results suggest that NSAIDs induces apoptosis of mice hepatoma, which may be due to their regulation on the expressions of bcl-2 family genes.  相似文献   

温度和湿度对伴性赤蚁sch系统致死性的影响   总被引：1，自引：1，他引：0  

卢忠燕  夏庆友  代方银  梁应霞 《蚕业科学》2003,29(2):125-130

为进一步探讨家蚕伴性赤蚁(sch)致死的机理,比较了不同温度、湿度催青条件下,sch系统及其杂交F_1代和对照种黑蚁系统的孵化率、失水率、含水率、失水临界温度等。结果表明:伴性赤蚁sch系统在已_3至已_4期对温湿度敏感,受到高温和干燥的冲击,将获得“孵化不能”性状;温度、湿度因子对赤蚁sch孵化抑制均有较大的独立作用,sch表现出一定的“温度敏感性”和“湿度敏感性”;温度、湿度都能通过影响胚胎体内水分平衡失调而引起这种孵化不能;在不引起水分变化的情况下,温度还存在一条独立的抑制赤蚁孵化的途径。  相似文献