Canine parvovirus outbreaks     

Palmer J  Thornley M 《Australian veterinary journal》2004,82(12):720-720

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D-lactate production and excretion in diarrheic calves     

Ewaschuk JB  Naylor JM  Palmer R  Whiting SJ  Zello GA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(5):744-747

The origin of D-lactate, the most important acid contributing to metabolic acidosis in the diarrheic calf, is unknown. We hypothesized that because D-lactate is produced only by microbes, gastrointestinal fermentation is the source. The objective of this study was to determine whether D-lactate production occurs in the rumen, colon, or both, and to measure D- and L-lactate concentrations in urine. Fecal, rumen, blood, and urine samples were obtained from 16 diarrheic and 11 healthy calves. Serum electrolyte concentrations were measured in both groups, and blood gas analyses were performed for diarrheic calves. All samples were analyzed for D- and L-lactate by high performance liquid chromatography (HPLC). Diarrheic calves were generally hyperkalemic with high serum anion gap, depressed serum bicarbonate, and low blood pH. L-lactate was markedly higher in rumen contents (22.7 mmol/ L [median]) and feces (8.6 mmol/L) of diarrheic calves than healthy calves (0.5 mmol/L and 5.1 mmol/L, respectively), but not different in serum or urine. Rumen, fecal, serum, and urine D-lactate concentrations were all significantly higher (P < .05) in diarrheic calves (17.0, 25.4, 13.9, and 19.2 mmol/L, respectively) than in healthy calves (0.5, 9.1, 1.4, and 0.5 mmol/L, respectively). Higher D-lactate concentrations in the rumen and feces of diarrheic calves suggests these sites as the source of D-lactate in blood and urine.  相似文献   

Antigen-specific proliferation and activation of peripheral blood mononuclear cells from Mycobacterium bovis-infected reindeer     

Waters WR  Palmer MV  Thacker TC  Minion FC  Davis WC 《Veterinary immunology and immunopathology》2006,111(3-4):263-277

To evaluate antigen-specific proliferative and activation-associated responses from Mycobacterium bovis-infected reindeer, blood mononuclear cells from M. bovis- (n = 10) and non-infected reindeer (n = 4) were stimulated with a recombinant early secretory antigenic target-6 and culture filtrate protein-10 fusion protein (rESAT6:CFP10), M. bovis purified protein derivative, pokeweed mitogen, or medium alone and evaluated by flow cytometry using dye tracker analysis and cell surface marker staining. gammadelta TCR+ and CD8+ cells, but not CD4+ cells, from M. bovis-infected reindeer proliferated in response to specific antigen stimulation. Expression (i.e., mean fluorescence intensity) of CD44 was increased and CD62L decreased on proliferative as compared to non-proliferative fractions in antigen- and mitogen-stimulated cultures. In response rESAT6:CFP10 stimulation, MHC II fluorescence intensity was increased on CD4+, gammadelta TCR+, CD172a+, and IgM+ cells from infected reindeer as compared to that of non-stimulated cells from the same reindeer. Recombinant ESAT6:CFP10 stimulation also induced expansion of a CD172a+, MHC II+ population within mononuclear cell cultures from M. bovis-infected reindeer. Despite a moderate challenge dose and extended duration of incubation, experimental infection of reindeer was generally limited to lymph nodes draining the inoculation site, suggestive of host resistance to progressive disease. Present in vitro findings, therefore, may be predictive of host responses by reindeer that limit progression to disseminated disease.  相似文献   

Effect of day and night temperature on the expression of male sterility of nuclear male-sterile (ms8ms8) soybean     

P. T. Perez-Sackett  R. G. Palmer 《Euphytica》2012,186(3):847-853

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Ecology. The heartbeat of ecosystems     

Palmer MA  Febria CM 《Science (New York, N.Y.)》2012,336(6087):1393-1394

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Immunoperoxidase study of adenovirus pneumonia in dogs     

R. Ducatellet  D. Palmer  P. Ossene  J. Hoorens 《The Veterinary quarterly》2013,33(4):290-296

Summary

The pathology of adenovirus pneumonia in 16 dogs is described. Clinically, these dogs had been severely ill, with severe dyspnoea and listlessness, but only faint coughing.

Histopathological lesions could be associated directly with the presence of adenovirus antigens in the lungs of these dogs by using an unlabelled immunoperoxidase technique on paraffin tissue sections. The lesions were focal and located in alveoli and bronchioles. Infected cells were mostly alveolar macrophages and less frequently type I and 2 pneumocytes and bronchiolar epithelial cells. Infiltrating neutrophils and lymphocytes were not observed to be infected.

This type of pneumonia appears to be a fairly well defined clinical and pathological entity in kennel dogs.  相似文献   

Gait and electromyographic alterations due to early onset of injury and eventual rupture of the cranial cruciate ligament in dogs: A pilot study     

Caroline P. Adrian  Kevin K. Haussler  Christopher E. Kawcak  Raoul F. Reiser  Cheryl Riegger‐Krugh  Ross H. Palmer  C. Wayne McIlwraith  Robert A. Taylor 《Veterinary surgery : VS》2019,48(3):388-400

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The effect of intravenous fresh frozen plasma administration on fibrinogen and albumin concentrations in sick neonatal foals          下载免费PDF全文

A. R. Hollis  P. A. Wilkins  B. Tennent‐Brown  J. E. Palmer  R. C. Boston 《Equine Veterinary Education》2016,28(3):145-149

This study investigated the immediate (6 h or less) effects of fibrinogen and albumin contained in transfused equine origin fresh frozen plasma on those proteins when measured in sick neonatal foals. Fibrinogen and albumin concentrations were measured in the administered plasma and in 31 sick foals at admission to a referral neonatal intensive care unit. Additional samples were obtained from the foals at 2 and 6 h following transfusion. No changes in albumin concentration were recognised. The main determinant of fibrinogen concentration following transfusion was the concentration of fibrinogen in the foal at admission. Importantly, intravenous transfusion of equine fresh frozen plasma did not result in immediate (6 h or less) increases or decreases in the fibrinogen concentration in the recipient foals. Fibrinogen from the donor contained within transfused plasma will not directly affect fibrinogen concentrations measured at later times.  相似文献   

In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections     

Mitchell?A.?Ellison  Michael?B.?McMahon  Morris?R.?Bonde  Cristi?L.?Palmer  Douglas?G.?LusterEmail authorView author&#;s OrcID profile 《Plant methods》2016,12(1):37

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Reconstructing past ocean pH-depth profiles   总被引：1，自引：0，他引：1  

MR Palmer  PN Pearson  SJ Cobb 《Science (New York, N.Y.)》1998,282(5393):1468-1471

Measurement of boron isotope compositions in species of planktonic foraminifera that calcified their tests at different depths in the water column are used to reconstruct the pH profile of the upper water column of the tropical ocean. Results for five time windows from the middle Miocene to the late Pleistocene indicate pH-depth profiles similar to that of the modern ocean in this area, which suggests that this method may greatly aid in our understanding of the global carbon cycle.  相似文献