ESLab application to a boreal watershed in southern Finland: preparing for a virtual research environment of ecosystem services     

Maria Holmberg  Anu Akujärvi  Saku Anttila  Lauri Arvola  Irina Bergström  Kristin Böttcher  Xiaoming Feng  Martin Forsius  Inese Huttunen  Markus Huttunen  Yki Laine  Heikki Lehtonen  Jari Liski  Laura Mononen  Katri Rankinen  Anna Repo  Vanamo Piirainen  Pekka Vanhala  Petteri Vihervaara 《Landscape Ecology》2015,30(3):561-577

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Copper localization in Cannabis sativa L. grown in a copper-rich solution     

Laura Arru  Sara Rognoni  Micaela Baroncini  Piera Medeghini Bonatti  Pierdomenico Perata 《Euphytica》2004,140(1-2):33-38

Summary With the aim to examine its potential as a renewable resource to decontaminate polluted soils, electron microscopy combined with X-ray microanalysis was used to investigate the localization of copper in Cannabis sativa grown in hydroponic copper-rich culture. Cu was found to accumulate preferentially in the upper leaf epidermal cells; it was also detected in spiculae and in abaxial trichomes too. Primary bast fibres seem to be not involved in copper accumulation.  相似文献   

Genetic transformation of Eustoma grandiflorum Griseb. by microprojectile bombardment     

Laura Semeria  Anna Maria Vaira  Gian Paolo Accotto  Andrea Allavena 《Euphytica》1995,85(1-3):125-130

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Trapping for mink control and water vole survival: Identifying key criteria using a spatially explicit individual based model     

Laura Bonesi  Steve P. Rushton 《Biological conservation》2007,136(4):636-650

Introduced alien species are the second most important threat to global biodiversity after habitat loss. The American mink Mustela vison has been introduced to several countries and is threatening a number of native species worldwide. We developed a spatially explicit and individual based model as a planning tool to identify key criteria for the implementation of trapping campaigns as a way to control open American mink populations. We first predicted the minimum effort required to reduce populations of mink below a certain threshold and the best time of year in which to trap mink to minimise their numbers. We then employed this methodology to predict the best trapping strategy to ensure the long-term survival of the water vole Arvicola terrestris, one of the species most endangered by the spread of the mink in the UK. We also applied the mink and water vole population models to rationalise a set of observed data in an area of 50 × 30 km in the Upper Thames (UK). The model predicted that it is necessary to remove mink for at least 3 months every year and that a mixed strategy of trapping during the mating, late dispersal and winter seasons is best for keeping mink at low densities. Concentrating trapping during the late dispersal and winter seasons is instead best for ensuring the long-term survival of water voles. Targeting immigrating juvenile mink as well as reproductive adults is important. The model also showed that trapping efficiency might be an important factor to consider when choosing periods in which to trap.  相似文献   

Comparison of methods to detect fecal streptococci in marine waters     

Laura Volterra  Lucia Bonadonna  Francesca Anna Aulicino 《Water, air, and soil pollution》1985,26(2):201-210

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Physiological and biochemical characterization of quinclorac resistance in a false cleavers (Galium spurium L.) biotype     

Van Eerd LL  Stephenson GR  Kwiatkowski J  Grossmann K  Hall JC 《Journal of agricultural and food chemistry》2005,53(4):1144-1151

The physiological and biochemical basis for quinclorac resistance in a false cleavers (Galium spurium L.) biotype was investigated. There was no difference between herbicide resistant (R) and susceptible (S) false cleavers biotypes in response to 2,4-D, clopyralid, glyphosate, glufosinate-ammonium, or bentazon. On the basis of GR(50) (growth reduction of 50%) or LD(50) (lethal dose to 50% of tested plants) values, the R biotype was highly resistant to the acetolactate synthase (ALS) inhibitor, thifensulfuron-methyl (GR(50) resistance ratio R/S = 57), and quinolinecarboxylic acids (quinclorac R/S = 46), resistant to MCPA (R/S = 12), and moderately resistant to the auxinic herbicides picloram (R/S = 3), dicamba (R/S = 3), fluroxypyr (R/S = 3), and triclopyr (R/S = 2). The mechanism of quinclorac resistance was not due to differences in [(14)C]quinclorac absorption, translocation, root exudation, or metabolism. Seventy-two hours after root application of quinclorac, ethylene increased ca. 3-fold in S but not R plants when compared to controls, while ABA increased ca. 14-fold in S as opposed to ca. 3-fold in R plants suggesting an alteration in the auxin signal transduction pathway, or altered target site causes resistance in false cleavers. The R false cleavers biotype may be an excellent model system to further examine the auxin signal transduction pathway and the mechanism of quinclorac and auxinic herbicide action.  相似文献   

Monitoring chemical changes of dry-cured Parma ham during processing by surface autofluorescence spectroscopy     

Møller JK  Parolari G  Gabba L  Christensen J  Skibsted LH 《Journal of agricultural and food chemistry》2003,51(5):1224-1230

Parma hams at various processing stages were investigated by surface autofluorescence spectroscopy. Fluorescence "landscapes" of raw meat and salted (3 months), matured (11 and 12 months), and aged (15 and 18 months) Parma hams were obtained, and a three-dimensional data array (sample x emission x excitation) was used to develop a PARAFAC model including five components, which all exhibited characteristics of pure fluorophores regarding both excitation and emission spectra. The relative amount of each component related strongly to the processing stage, and sample age showed good correlation to fluorescence data (R = 0.98), with a relative error of prediction of approximately 1 month. Fluorescence measurements from samples of either semimembranosus or biceps femoris were used to predict chemical or sensory reference data, yielding good correlation for biceps femoris data, thereby enabling moisture content, sensory and instrumental color, and proteolysis value to be fairly well predicted. Overall, surface autofluorescence of Parma hams proved to hold relevant information, relating to major chemical/physical changes during processing. It is concluded that fluorescence spectroscopy has potential as an innovative method of quality control in dry-cured ham.  相似文献   

Comparative effects of food-derived polyphenols on the viability and apoptosis of a human hepatoma cell line (HepG2)     

Ramos S  Alía M  Bravo L  Goya L 《Journal of agricultural and food chemistry》2005,53(4):1271-1280

Consumption of fruits and vegetables, which are rich in polyphenols, has been associated with a reduced risk of chronic diseases such as cancer. Dietary polyphenols have antioxidant and antiproliferative properties that might explain their beneficial effect on cancer prevention. The aim of this study was to investigate the effects of different pure polyphenols [quercetin, chlorogenic acid, and (-)-epicatechin] and natural fruit extracts (strawberry and plum) on viability or apoptosis of human hepatoma HepG2 cells. The treatment of cells for 18 h with quercetin and fruit extracts reduced cell viability in a dose-dependent manner; however, chlorogenic acid and (-)-epicatechin had no prominent effects on the cell death rate. Similarly, quercetin and strawberry and plum extracts, rather than chlorogenic acid and (-)-epicatechin, induced apoptosis in HepG2 cells. Moreover, quercetin and fruit extracts arrested the G1 phase in the cell cycle progression prior to apoptosis. Quercetin and strawberry and plum extracts may induce apoptosis and contribute to a reduced cell viability in HepG2 cells.  相似文献   

Accurate determination of 2,4,6-trichloroanisole in wines at low parts per trillion by solid-phase microextraction followed by GC-ECD   总被引：1，自引：0，他引：1  

Alzaga R  Ortiz L  Sánchez-Baeza F  Marco MP  Bayona JM 《Journal of agricultural and food chemistry》2003,51(12):3509-3514

A headspace solid-phase microextraction (HS-SPME) procedure at 30 degrees C with a 100 microm PDMS fiber of a saturated NaCl solution stirred at 1100 rpm combined to GC-ECD for the 2,4,6-trichloroanisol (TCA) determination in wines has been developed. Due to the matrix complexity and ethanol absorption into the fiber, the internal standard selection was crucial to obtain unbiased results. Thus, matrix effects were observed when analyzing different types of Spanish wines (white, early, and vintage red wines) spiked with TCA at low concentration levels (i.e., <40 ng L(-)(1)). In contrast, the use of 2,4,6-tribromoanisole (TBA) as internal standard overcame these matrix effects, whereas the use of 2,4,6-trichlorophenyl ethyl ether led to inconsistent results. The developed HS-SPME-GC-ECD methodology reaches a limit of quantitation for TCA in wine within 2.9-18 ng L(-)(1), with a relative standard deviation of 2.5-13.4%, depending on the TCA concentration level and wine characteristics. This analytical method is comparable to the existing methodologies based on HS-SPME followed by GC-MS in terms of accuracy, precision, length of determination, and length of quantification; however, analysis cost is reduced.  相似文献   

Assessing the Influence of Different Atmospheric and Soil Mercury Concentrations on Foliar Mercury Concentrations in a Controlled Environment     

Laura Fay  Mae Gustin 《Water, air, and soil pollution》2007,178(1-4):373-384

This study vividly presents results from a seasonal particulate matter measurement campaign conducted at world’s largest ship-breaking yard i.e., Alang-Sosiya (Gujarat, India) at six locations and a reference station at Gopnath which is 30 km south of this ship-breaking yard. The collected suspended particulate matter (SPM) 24-h samples were critically analyzed for heavy metals (Pb, Cd, Co, Ni, Cr, Mn, Fe, Cu, Zn). The average concentration of SPM within the ship-breaking yard during the investigation was 287.5 ± 20.4 μg m^?3 and at reference station it was 111.13 ± 5.81 μg m^?3. These values are found to be in excess of the permitted national standards. The levels of heavy metals at Alang-Sosiya are very high as compared to US EPA and WHO guidelines. The mean concentrations of all metals are in the order: Fe >>Zn >Cu > Mn > Cd >Pb > Co >Ni >Cr. The results on enrichment factors (EF) suggest that most of the metals in the ship-breaking yard exhibit EF values of near or above 100 which must have been comprehensively affected by ship-breaking activities. Metal data was used to evaluate the role of spatial factors on their distribution characteristics. Thereafter, factor analysis was carried out to identify the main components liable for the variance of the data set.  相似文献