Prospective evaluation of minimally invasive plate osteosynthesis in 36 nonarticular tibial fractures in dogs and cats     

Guiot LP  Déjardin LM 《Veterinary surgery : VS》2011,40(2):171-182

Objective: To evaluate the clinical outcome of minimally invasive plate osteosynthesis (MIPO) for nonarticular tibial fractures stabilized using bone plates with or without an intramedullary rod (IMR). Study Design: Prospective study. Animals: Dogs (n=28) and 8 cats. Methods: After closed reduction, fracture fixation was achieved using an epiperiosteal plate inserted percutaneously through epiphyseal small incisions. In some fractures, an IMR was inserted via medial parapatellar arthrotomy. Radiographs were recommended every 3 weeks until clinical union. Postoperative tibial length and alignment were compared with contralateral measurements (P<.05). Time to clinical union and complications were recorded. Results: An IMR was used in 30.5% of the cases. Repaired tibiae were 1% shorter than contralateral tibiae (P<.05). Frontal and sagittal alignment were similar between repaired and contralateral tibiae (P>.05). Six dogs were lost for follow‐up; owners indicated normal function. In 30 cases for which bone healing was documented, mean±SD healing time was 45±20.8 days; however, when considering the 23 cases, which completed preestablished scheduled follow‐ups, healing time was 36±11.6 days. Minor complications occurred in 4 cases (11%). One major complication (3%) consisting of a plate fracture was successfully revised using MIPO with a larger plate. Conclusions: Consistent restoration of alignment was accomplished using MIPO techniques. Furthermore, MIPO appeared to yield faster healing times and lower complication rates than those reported with conventional plate osteosynthesis.  相似文献   

Impact of ultrafiltration membrane material on Peptide separation from a snow crab byproduct hydrolysate by electrodialysis with ultrafiltration membranes     

Doyen A  Beaulieu L  Saucier L  Pouliot Y  Bazinet L 《Journal of agricultural and food chemistry》2011,59(5):1784-1792

Electrodialysis with ultrafiltration membrane (EDUF) is a technology based on the separation of molecules according to their charge and molecular mass. Some works have already successfully demonstrated the recovery of bioactive peptide fractions. However, the impact of ultrafiltration membrane (UFM) material, used in the EDUF system, on the peptide migration has never been studied. Consequently, the objectives of this work were (1) to evaluate the effect of two different UFM materials on the selective separation of peptides from a snow crab byproduct hydrolysate by electrodialysis with ultrafiltration membranes and (2) to determine the effect of UFM material on their potential fouling by peptides. It appeared that, after 6 h of EDUF separation using polyether sulfone (PES) and cellulose acetate (CA) UFM, peptides with low molecular weights ranging from 300 to 700 Da represented the most abundant population in the KCl1 (compartment located near the anode for the recovery of anionic/acid peptide fractions) and KCl2 (compartment located near the cathode for the recovery of cationic/basic peptide fractions) permeates. Peptides with molecular weights ranging from 700 to 900 Da did not migrate during the EDUF treatment. Moreover, only CA UFM allowed the recovery of high molecular weight molecules (900-20000 Da) in both KCl compartments. Peptides desorbed from PES and CA UFM after 6 h of EDUF separation had low molecular weights and belonged mainly to the 600-700 Da molecular weight range. These peptides represented a low proportion of the peptides initially present in the snow crab byproduct hydrolysate with individual molecular weight range proportions from 1.52 ± 0.31 to 10.2 ± 2.32%.  相似文献   

Characterization of Pectobacterium brasiliense strains from potato and vegetables in Israel     

Leah Tsror  Valerie Hélias  Sara Mordechai-Lebiush  Orly Erlich  Marina Hazanovsky  Laura Chalupowicz  Michal Reuven  Orit Dror  Lea Valinsky  Angelique Laurent  Shulamit Manulis-Sasson 《Plant pathology》2021,70(9):2179-2187

Pectobacterium brasiliense (Pbr) infects a wide range of crops worldwide, causing potato blackleg and soft rot and vegetable soft rots. This study aimed to characterize the genetic diversity and virulence variability among 68 Pbr strains isolated from either symptomless potato progeny tubers, diseased potato plants, ware potatoes wash water, or vegetables grown in Israel, as well as strains isolated from symptomless seed tubers grown in Europe, or diseased potato plants grown in France. The collection was typed using PCR and TaqMan real-time PCR analyses, dnaX sequence analysis, pulsed-field gel electrophoresis (PFGE), and pectolytic activity. dnaX phylogeny grouped almost all strains in a common genetic clade related to Pbr, which was distinct from the other Pectobacterium species. PFGE analysis identified two main clusters, including one major group of 47 strains with 95%–100% similarity. Maceration assays on two potato cultivars showed significant differences between strains but with no correlations with the source of the strains nor the status of the host (with/without symptoms). Molecular (dnaX sequences and PFGE profiles) and phenotypic analyses (tuber maceration tests) showed that the tested Pbr strains are not a homogeneous group. Analysis of the tested Pbr strains isolated from potato and vegetables grown in fields with a history of potato cultivation suggests that seed tubers imported from Europe may be the main source for Pbr in Israel. To the best of our knowledge, this is the first study that describes biodiversity and population structure of P. brasiliense isolated from potato and vegetables under hot climate conditions.  相似文献   

Gemvid, an open source, modular, automated activity recording system for rats using digital video     

Jean-Etienne Poirrier  Laurent Poirrier  Pierre Leprince  Pierre Maquet 《Journal of circadian rhythms》2006,4(1):10-10

Background  

Measurement of locomotor activity is a valuable tool for analysing factors influencing behaviour and for investigating brain function. Several methods have been described in the literature for measuring the amount of animal movement but most are flawed or expensive. Here, we describe an open source, modular, low-cost, user-friendly, highly sensitive, non-invasive system that records all the movements of a rat in its cage.  相似文献   

Arbuscular Mycorrhizal Fungi Alleviate the Negative Effect of Temperature Stress in Millet Lines with Contrasting Soil Aggregation Potential     

Ndeko  Adrien Byamungu  Founoune-Mboup  Hassna  Kane  Aboubacry  Cournac  Laurent 《Gesunde Pflanzen》2022,74(1):53-67

Arbuscular mycorrhizal fungi (AMF) establish a mutualistic symbiosis with several plants and play a key role in improving plant growth, tolerance to abiotic and biotic stresses as well as the soil structure. This work aimed at elucidating the AMF temperature stress modulating impact on four pearl millet lines plant growth and soil aggregation. Experimental trials were carried out in both greenhouse and growth chamber to determine the response of the four millet lines to inoculation with two AMF strains (Rhizophagus aggregatus and Funneliformis mosseae) under heat and non-stress conditions. We first investigated the mycorrhizal colonization (MC) and the mycorrhizal growth response (MGR) of millet lines in relation with their soil aggregation potential (root adhering soil/root biomass, MAS/RB) in the greenhouse. Secondly, the four millet lines were grown in two separated growth chambers and subjected to a day/night temperature of 32/28?°C as the control treatment and 37/32?°C as the temperature stress treatment. Plant growth, mycorrhization rate and several physiological, mycorrhizal and soil parameters were measured. Results showed that the mycorrhization rates of millet lines were low and not significantly different. Funneliformis mosseae (31.39%) showed higher root colonization than Rhizophagus aggregatus (22.79%) and control (9.79%). The temperature stress reduced the mycorrhizal colonization rate, shoot and root biomass, and the soil aggregation for all tested lines. L220 and L132 showed more MC rate and MGR than the other lines under control and high-temperature treatment. The MGR was significantly better under temperature stress conditions than in the control. Under the temperature stress conditions, inoculation with R. aggregatus and F. mosseae increased chlorophyll concentration, root dry weight and shoot dry weight as compared to non-inoculated plants. AMF inoculation, particularly with F. mosseae had a positive influence on the tolerance of millet lines to temperature stress. This study demonstrates that AMF play an important role in the response of these four millet lines to temperature stress. AMF is therefore an important component in the adaptation of crops to climatic variations in Sub-Saharan Africa.

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Impact of the potato inoculation date on potato virus Y load and viral distribution in daughter tubers at harvest     

Frédéric Boulard  Gallien Rohaut  David Dupré  Florian Barbe Barrailh  Amélie Person  Melen Leclerc  Emmanuel Jacquot  Laurent Glais 《Plant pathology》2022,71(6):1323-1334

Aged plants are more difficult to infect than young plantlets. This modification of susceptibility is described as mature plant resistance (MPR). For potato virus Y (PVY), MPR is known to lead to low infection rates of plants inoculated at the postflowering stage and a decrease in the number of infected daughter tubers. However, the impact of inoculation date on the capacity of PVY to accumulate in daughter tubers has not been studied so far. Field and greenhouse experiments were carried out to better understand PVY epidemiology and to help potato growers to evaluate consequences of early/late infections on the quality of their crops. In field trials, potato plants (cv. Bintje) were covered by insectproof nets from planting to harvest except for a 14-day period to expose plants to natural PVY infections. Under controlled conditions, potato plants were mechanically inoculated with PVY at different dates from preflowering stages (early inoculations) to postflowering stage (late inoculations). At harvest, daughter tubers were individually collected and analysed to define proportions and viral load of infected tubers according to the time between virus inoculation and harvest. Our results showed that although the age of plants at the time of inoculation can modify their susceptibility to PVY infection, in return, early and late PVY inoculations lead to similar rates of infected tubers at the plant scale and equivalent viral accumulation in infected tubers. All together, these data revealed that both early/late infections are high risks for the sanitary quality of potato tubers.  相似文献   

Anticancer Alkaloid Lamellarins Inhibit Protein Kinases          下载免费PDF全文

Dianne Baunbk  Nolwenn Trinkler  Yoan Ferandin  Olivier Lozach  Poonsakdi Ploypradith  Somsak Rucirawat  Fumito Ishibashi  Masatomo Iwao    Laurent Meijer 《Marine drugs》2008,6(4):514-527

Lamellarins, a family of hexacyclic pyrrole alkaloids originally isolated from marine invertebrates, display promising anti-tumor activity. They induce apoptotic cell death through multi-target mechanisms, including inhibition of topoisomerase I, interaction with DNA and direct effects on mitochondria. We here report that lamellarins inhibit several protein kinases relevant to cancer such as cyclin-dependent kinases, dual-specificity tyrosine phosphorylation activated kinase 1A, casein kinase 1, glycogen synthase kinase-3 and PIM-1. A good correlation is observed between the effects of lamellarins on protein kinases and their action on cell death, suggesting that inhibition of specific kinases may contribute to the cytotoxicity of lamellarins. Structure/activity relationship suggests several paths for the optimization of lamellarins as kinase inhibitors.  相似文献   

An explicit stem profile model for forked and un-forked teak (Tectona grandis) trees in West Africa     

Stephen Adu-Bredu  Alphonse Foua Tape Bi  Jean-Pierre Bouillet  Martial Kouamé Mé  Samuel Yamoah Kyei  Laurent Saint-André 《Forest Ecology and Management》2008

Studies on stem profile of teak (Tectona grandis) hardly exist. This can be possibly attributed to the occurrence of forks that is prevalent on teak trees. Stem profile model was therefore developed for teak in West Africa, which took into account the occurrence of forks. Trees were destructively sampled from Moist Evergreen Forest (MEF), Moist Semi-deciduous Forest (MSDF), Dry Semi-deciduous Forest (DSDF) and Savannah ecological zones in both Ghana and Côte d’Ivoire for the study. A single set of parameters could be used explicitly to predict stem profile of any teak tree, irrespective of country, eco-climatic zone, edaphic variables, site index, tree age, stand density and tree competition. The model efficiency and root mean square error (in relative diameter) were calculated to be 0.97 and 0.053, respectively. The relative position of the forks along the stem bole, with respect to total tree height, was highly variable and did not follow any particular trend, except higher value for the first fork in the Moist Evergreen Forest zone. However, average tree height to the first fork decreased along the ecological gradient from MEF, MSDF and DSDF to Savannah. The extent of diameter reduction due to the forks could be predicted. Relative tree form for zero-forked, one-forked and two-forked trees was calculated to be 0.39, 0.36 and 0.33, respectively. Relative loss of stem volume due to one fork and two forks was estimated to be 6.5 and 13.9%, respectively. The measured stem volume when related to the corresponding predicted value yielded coefficient and intercept not significantly different from unity and zero, respectively, with an r² value of 0.97.  相似文献   

Evaluation of the visual stress grading standard on French Spruce (Picea excelsa) and Douglas-fir (Pseudotsuga menziesii) sawn timber     

Guillaume Roblot  Damien Coudegnat  Laurent Bleron  Robert Collet 《Annals of Forest Science》2008,65(8):812-812

? In this paper an evaluation of the visual grading standard for softwood sawn timber was made.

? In order to do so, visual grading according to EN 518 and theoretical grading according to EN 338 and EN 384 (measurements of MOE, MOR and density) were applied to lumber. Two batches of 111 and 102 French boards were graded, respectively, of Spruce (Picea excelsa) and Douglas-fir (Pseudotsuga menziesii). For the visual grading the most discriminant criterion was noted: knots, cracks, wane, etc.

? Finally, the results of the two grading methods were compared, and it was shown that the visual stress grading gave quite low results for our two French species.

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Chitosan oligomers and copper sulfate induce grapevine defense reactions and resistance to gray mold and downy mildew     

Aziz A  Trotel-Aziz P  Dhuicq L  Jeandet P  Couderchet M  Vernet G 《Phytopathology》2006,96(11):1188-1194

ABSTRACT Chitosan (CHN), a deacetylated derivative of chitin, was shown to be efficient in promoting plant defense reactions. CHN oligomers of different molecular weight (MW) and degree of acetylation (DA) triggered an accumulation of phytoalexins, trans- and cis-resveratrol and their derivatives epsilon-viniferin and piceid, in grapevine leaves. Highest phytoalexin production was achieved within 48 h of incubation with CHN at 200 mug/ml with an MW of 1,500 and a DA of 20% (CHN1.5/20), while oligomers with greater MW were less efficient, indicating that a specific MW threshold could be required for phytoalexin response. Treatment of grapevine leaves by highly active CHN1.5/20 also led to marked induction of chitinase and beta-1,3-glucanase activities. CHN1.5/20 applied together with copper sulfate (CuSO(4)) strongly induced phytoalexin accumulation. CuSO(4) alone, especially at low concentrations also elicited a substantial production of phytoalexins in grapevine leaves. Evidence is also provided that CHN1.5/20 significantly reduced the infection of grapevine leaves by Botrytis cinerea and Plasmopara viticola, and in combination with CuSO(4) conferred protection against both pathogens.  相似文献