Isoform-specific modulation of Na+, K+-ATPase alpha-subunit gene expression in hypertension   总被引：6，自引：0，他引：6  

V L Herrera  A V Chobanian  N Ruiz-Opazo 《Science (New York, N.Y.)》1988,241(4862):221-223

Sodium, potassium-adenosine triphosphatase (Na+, K+-ATPase) is hypothesized to be involved in systemic vascular hypertension through its effects on smooth muscle reactivity and myocardial contractility. By means of RNA blot analyses of cardiac, aortic, and skeletal muscle RNAs in two rat hypertensive models, Na+,K+-ATPase alpha-subunit messenger RNA isoforms (alpha 2 and alpha 3) were shown to be deinduced in response to increased intravascular pressure. The changes were observed after 48 hours or more of experimental hypertension. Under these conditions, there is coordinate induction of another alpha isoform (alpha 1) and of beta-subunit messenger RNAs, probably in response to alterations in sodium flux rather than to elevated blood pressure.  相似文献   

Effects on yield and nutrition of mycorrhizal and nodulated Pueraria phaseoloides exerted by P-solubilizing rhizobacteria     

M. Toro  R. Azcón  R. Herrera 《Biology and Fertility of Soils》1996,21(1-2):23-29

We studied the effect of bacteria involved in rock phosphate (four isolates), iron phosphate (two isolates), and aluminium phosphate (two isolates) solubilization, and two phytate-mineralizing bacteria in terms of their interaction with two Glomus spp. on Pueraria phaseoloides growth and nutrition. The plant —Rhizobium sp. — mucorrhiza symbiosis system may increase in yield and nutrition in association with specific rhizosphere bacteria that solubilize calcium, iron, and aluminium phosphates. No benefit from phytate-mineralizing bacteria was found under these experimental conditions. P. phaseloides growth responses were influenced in different ways by specific combinations of the selected bacteria and arbuscular mycorrhizal fungi. Considerable stimulation of nutrient uptake was observed with fungus-bacteria combinations of Azospirillum sp. 1, Bacillus sp. 1 or Enterobacter (spp. 1 or 2) associated with G. mosseae. The fact that Bacillus sp. 1, a calcium-phosphate solubilizing isolate, positively interacted with G. mosseae and negatively with G. fasciculatum is an indication of specific functional compatibility between the biotic components integrated in the system. From our results, the interactions between bacterial groups able to solubilize specific phosphate and mycorrhizal fungi cannot be interpreted as occurring only via P solubilization mechanisms since no generalized effect was obtained. Iron-phosphate solubilizing microorganisms were more active alone than in dual associations with Glomus sp., but the aluminium-phosphate dissolving isolates positively interacted in mycorrhizal plants. Further work is needed in this area in order to elucidate the mechanisms that affect rhizosphere microorganism interactions. G. mosseae was more effective but less infective than G. fasciculatum in most of the combined treatments.  相似文献   

Genotypic diversity of Salmonella ser. Abortusequi isolates from Argentina     

C. P. Bustos  M. Moroni  M. I. Caffer  A. Ivanissevich  M. Herrera  A. R. Moreira  N. Guida  P. Chacana 《Equine veterinary journal》2020,52(1):98-103

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Reproductive performance and seasonal plasma sex steroid and metabolite levels in a captive wild broodstock of brill Scophthalmus rhombus L.     

Ismael Hachero-Cruzado  Ángel García-López  Marcelino Herrera  Luis Vargas-Chacoff  Gonzalo Martínez-Rodríguez  Juan M Mancera  & José I Navas 《Aquaculture Research》2007,38(11):1161-1174

This study reports egg production by captive wild brill Scophthalmus rhombus, a potential new flatfish species for Southern Europe‐Mediterranean mariculture, as well as seasonal plasma levels of 17β–estradiol, testosterone, 11–ketotestosterone, proteins, triglycerides, glucose and lactate. A mean egg production of 102 800 eggs kg body weight⁻¹ was achieved during the 2005 spawning period (January–March), although a continuous egg supply could only be obtained from some females, which had a higher relative fecundity (261 019±10 393 eggs kg⁻¹) with 12–17 eggs batches released at a mean interval of 3.4 days. Most eggs were obtained with water temperatures ranging from 12 to 14°C, and under increasing temperatures (up to 2.9°C). Potential egg viability (70.1±2.9%), fertilization (72.2±3.4%) and hatching rates (31.9±3.9%) showed high variability, with potential viability tending to decrease as the water temperature increased (mainly between 16 and 17°C) and 0% hatching above 16.6°C. The endocrine changes that brill underwent during late gametogenesis, spawning and postspawning periods were similar to those reported in other Pleuronectiformes. This study establishes an important basis for further research on the biology and physiology of brill reproduction, directed towards the optimization of the breeding techniques used currently.  相似文献   

Determination of pesticides and PCBs in virgin olive oil by multicolumn solid-phase extraction cleanup followed by GC-NPD/ECD and confirmation by ion-trap GC-MS     

Yagüe C  Bayarri S  Conchello P  Lázaro R  Pérez-Arquillué C  Herrera A  Ariño A 《Journal of agricultural and food chemistry》2005,53(13):5105-5109

A multicolumn solid-phase extraction cleanup for the determination of organophosphorus (OP) and organochlorine (OC) pesticides plus PCB congeners in virgin olive oil is presented. The method involves dissolution of the olive oil in hexane, followed by a cleanup system using a diatomaceous earth column (Extrelut-QE) with reversed (C(18)) and normal (alumina) phase SPE columns. Determination of OPs was by GC-NPD, while the OCs and PCBs were analyzed using GC-ECD. Recovery assays for OPs varied from 81.7% to 105.3%, for OCs ranged between 74.3% and 99.4%, while for PCBs were from 60.1% to 119.2%. Quantitation limits ranged from 10 to 25 microg/kg olive oil for OPs, and from 1 to 6 microg/kg olive oil for OCs and PCBs. In the case of positive samples, the confirmation of pesticide identity was performed by ion-trap GC-MS/MS. The applicability of the method was assayed with 19 virgin olive oil samples collected from different olive mills of Aragón (Spain). Only one OP pesticide (acephate) was detected in one sample at a concentration of 10 microg/kg. Organochlorine pesticides were found in 5-47% of samples at very low levels ranging from 1.5 to 5.2 microg/kg. PCBs were found in 20-90% of samples, showing concentrations between 2.3 and 17.3 microg/kg.  相似文献   

Matrix effects on plant-frugivore and plant-predator interactions in forest fragments     

José M. Herrera  Daniel García  Juan M. Morales 《Landscape Ecology》2011,26(1):125-135

Structural features of both habitat remnants and surrounding matrix can be important for explaining plant population dynamics and ecosystem functions in human-impacted landscapes. However, little is known about how the structural features of the adjacent matrix affect biotic interactions and whether such context effects are subject to temporal variations. Using the hawthorn Crataegus monogyna in northern Spain, we studied matrix effects on two sequential plant-animal interactions, frugivory by birds and postdispersal seed predation by rodents. Using Hierarchical Linear Models, we compared the magnitude of both interactions on trees located in two patch types that strongly differed in structural features of the adjacent matrix habitat: patches totally surrounded by a degraded, structurally contrasted pastures (unconnected patches), and trees growing in patches adjacent to a lowly degraded, structurally similar mature forests (connected patches). We compared outcomes for 2005 and 2006, which were years with strong differences in community-wide fruit and seed abundance. Frugivory rate did not differ between patch types in either year, likely related to high mobility of birds. Seed predation rates were higher in unconnected patches than in connected ones, but only in 2005. We conclude that strong interannual fluctuations in resource availability are not rare in temperate systems and that recruitment rates could be frequently reduced within unconnected patches, thus collapsing plant regeneration processes of hawthorn populations. Overall, our results suggest that generalizations about potential effects of the matrix on plant-animal interactions within remnant patches must consider: (1) species-specific habitat responses of the organisms, (2) suitability of neighbouring habitats in terms of food supply, and (3) temporal variations in plant-resource availability for interacting animals.  相似文献   

Multihorned character in goats associated with an eyelid deformity     

Herrera M  Monteagudo LV  Tejedor MT  Arruga MV  Sierra I 《The Veterinary record》2007,161(5):176

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Functional Properties of Prickly Pear Cactus Fruit Peels Undergoing Supplemental Irrigation and Fruit Storage Conditions     

Herrera  Mayra Denise  Zegbe  Jorge A.  Melero-Meraz  Valentín  Cruz-Bravo  Raquel K. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2021,76(4):427-433

Plant Foods for Human Nutrition - Prickly pear cactus fruit peels have been seen as organic waste. This study explored the effect of supplemental irrigation during fruit growth of ‘Roja...  相似文献   

Rehabilitation of Nothofagus pumilio forests in Chilean Patagonia: can fencing and planting season effectively protect against exotic European hare browsing?     

Huertas Herrera  Alejandro  Promis  Álvaro  Toro-Manríquez  Mónica  Lencinas  María Vanessa  Martínez Pastur  Guillermo  Río  Matías 《New Forests》2022,53(3):469-485

New Forests - In forests affected by heavy fires and continuous grazing of exotic herbivorous mammal species, Nothofagus pumilio (lenga) cannot recover naturally. The main factors that hinder the...  相似文献   

Production of Xylose from Sorghum Straw Using Hydrochloric Acid   总被引：1，自引：0，他引：1  

A. Herrera  S. J. Tllez-Luis  J. A. Ramírez  M. Vzquez 《Journal of Cereal Science》2003,37(3):267-274

Xylose is a hemicellulosic sugar mainly used for its bioconversion to xylitol. Sorghum straw is a raw material for xylose production that has been studied scarcely. The objective of this work was to study the xylose production by hydrolysis of sorghum straw with hydrochloric acid at 122 °C. Several concentrations of HCl (2–6%) and reaction time (0–300 min) were evaluated. Kinetic parameters of mathematical models for predicting the concentration of xylose, glucose, acetic acid and furfural in the hydrolysates were found. Optimal conditions for hydrolysis were 6% HCl at 122 °C for 70 min, which yielded a solution with 16·2 g xylose/L, 3·8 g glucose/L, 2·0 g furfural/L and 1·9 g acetic acid/L.  相似文献