A highly accurate, single PCR reaction for parentage assignment in Senegal sole based on eight informative microsatellite loci     

Roberto de la Herrán  Francisca Robles  José Ignacio Navas  Abdel Mounim Hamman-Khalifa  Marcelino Herrera  Ismael Hachero  María José Mora  Carmelo Ruiz Rejón  Manuel Garrido-Ramos  & Manuel Ruiz Rejón 《Aquaculture Research》2008,39(11):1169-1174

From a battery of microsatellite markers (100 loci), recently identified by our group, we have selected eight for parentage assignment in Senegal sole ( Solea senegalensis ). This tool is based on microsatellite loci obtained from four genomic DNA libraries and one cDNA library. Within the eight loci (six from anonymous genomic DNA sequences and two located in expressed sequence tags of known genes), we have found, in an analysis of a reproductive broodstock, between nine and 16 alleles. The expected heterozygosity was between 0.616 and 0.860. In addition, we have optimized the polymerase chain reaction (PCR) conditions to amplify all loci simultaneously in a single multiplex PCR reaction, and we have tested three lots of male and female (five to six individuals) and three offspring (50–60 larvae each). The use of the eight microsatellite loci, the possibility of amplifying them in a single PCR reaction and the high value of the exclusion probability (0.9992) make this multiplex PCR method a unique tool for parentage assignment.
Finally, analysing one meiotic gynogenetic progeny, we have determined the relative distance of six of these loci to the centromere, and we have also found that all of them are unlinked. All these characteristics confer this tool with a high accuracy for parentage studies and genetic population analyses of Senegal sole.  相似文献   

Metabolic enzyme activities in relation to crowding stress in the wedge sole (Dicologoglossa cuneata)          下载免费PDF全文

Marcelino Herrera  Ignacio Ruiz‐Jarabo  Luis Vargas‐Chacoff  Elena de la Roca  Juan M Mancera 《Aquaculture Research》2015,46(11):2808-2818

In this work, we have assessed the effects of different stocking densities on the biometry, survival and physiological status of the wedge sole (Dicologoglossa cuneata), focusing on changes in the stress system and intermediate metabolism, with the aim of determining a stress indicator for chronic‐stress situations in this species. Wedge sole were kept at three different stocking densities (0.2, 0.4 and 0.8 kg m^?3) for 197 days, and survival, growth, plasma metabolites, cortisol and enzymatic activities were assessed. Survival rates were the highest at low density, though growth did not vary significantly among treatments. Enzymatic activities, mainly in muscle, differed depending on stocking density. Liver hexokinase activity at low stocking density was the highest, while no differences were detected for the other enzymes assessed. In muscle, all enzymes significantly increased in activity with stocking density. We concluded that long‐term high stocking density culture significantly changed enzyme activities (hexokinase, glutamate dehydrogenase, glyceraldehyde 3‐phosphate dehydrogenase, and lactate dehydrogenase) in wedge sole muscle, although liver enzymes, plasma cortisol and metabolites did not vary significantly. Therefore, muscle enzymes, hexokinase and glutamate dehydrogenase, could be considered stress indicators for this species in chronic‐stress situations.  相似文献   

Effects of two diets on lipid composition and reproductive performance of brill (Scophthalmus rhombus) eggs     

Ismael Hachero‐Cruzado  Pilar Olmo  Beatriz Sánchez  Marcelino Herrera  Pedro Domingues 《Aquaculture Research》2012,43(10):1439-1450

The effects of two diets with different lipid composition (squid Loligo gahi and one commercial pellet for breeders) on reproductive performance and egg lipid composition of brill (Scophthalmus rhombus) were compared. A total of 36 adult brill weighing 1482 ± 432 g were randomly distributed in the experimental tanks. A flow‐through system composed of four tanks with 4.2 m² of bottom area (5.0 m³ × 1.2 m water depth) was used. The experiment lasted for approximately 5 months (168 days). From the four mature females fed squid, three ovulated. Five females fed pellets matured and ovulated. Total ovulations were of 10 and 44, by the three females fed squid and the five ovulating female fed pellets respectively. Egg viability was higher, although not significantly, for females fed pellets. This indicates that pellets could be a better food for breeding females. In general, the egg total lipid content and the lipid classes composition did not seem to be affected by diet. In contrast, results indicate that not only diets but also individual females, regardless of the diet consumed, are determinant for egg fatty acid composition, clearly modulating their composition.  相似文献   

Live prey first feeding regimes for short‐snouted seahorse Hippocampus hippocampus (Linnaeus, 1758) juveniles     

Francisco Otero‐Ferrer  Lucía Molina  Juan Socorro  Rogelio Herrera  Hipólito Fernández‐Palacios  María Soledad Izquierdo 《Aquaculture Research》2010,41(9):e8-e19

As with many species of seahorses, Hippocampus hippocampus wild populations are being subjected to uncontrolled exploitation in their natural environment. Thus, aquaculture could contribute to satisfy the commercial demand for animals while promoting the recovery of wild stocks. The present study was conducted to compare the effect of the substituting Artemia nauplii with rotifers for first feeding seahorse juveniles. Survival, growth and biochemical composition of prey organisms and fish were studied during the feeding trial. In addition, to help the biometric study, an anaesthetic test was also carried out using clove oil. The results showed excellent survival (average 60%) in juveniles exclusively fed with Artemia, with better values than those reported previously obtained by other authors for this species. By comparison, high mortality and poor growth were observed during first feeding with seahorses fed on rotifers. This could have been related to the lower energy intake and poorer nutritional value of the rotifers. Furthermore, clove oil concentrations of 25 ppm were found to work well as an anaesthetic for seahorse juveniles. Overall, first feeding Artemia alone was found to be an efficient and simplified method for feeding young H. hippocampus fry, building the principles for their culture for ornamental or re‐stocking purposes.  相似文献   

Entamoeba sp. (Sarcomastigophora: Endamoebidae) from ostriches (Struthio camelus) (Aves: Struthionidae)     

Martínez-Díaz RA  Herrera S  Castro A  Ponce F 《Veterinary parasitology》2000,92(3):173-179

The first case of Entamoeba of the 1-nucleate mature cyst group in birds is described. Trophozoites and cysts of Entamoeba have been found in ostriches (Struthio camelus) from farms located all over Spain. The cysts are large (13.47microm mean diameter); they possess one nucleus when mature, with a large endosome and peripheral chromatine arranged in small granules; chromatoid bodies, when present, are large and elongated. Trophozoites are large (19. 88microm mean diameter), with a clear differentiation between ecto- and endoplasm, this containing numerous vacuoles; the nucleus is large and diffuse. The characteristics of this amoeba resembles but do not completely fulfill those of E. suis and E. chattoni; also, these species are from mammals.  相似文献   

Study of Calcium Ion Diffusion in Nixtamalized Quality Protein Maize as a Function of Cooking Temperature     

E. Gutierrez  I. Rojas-Molina  J. L. Pons-Hernandez  H. Guzman  B. Aguas-Angel  J. Arenas  P. Fernandez  A. Palacios-Fonseca  G. Herrera  M. E. Rodríguez 《Cereal Chemistry》2007,84(2):186-194

In this report, the effect of temperature on the calcium content of Quality Protein Maize (QPM H-368C) during the nixtamalization process as a function of the steeping time for three cooking temperatures (72, 82, and 92°C) is presented. Also, for the first time, we report in physico-chemical terms the end of the cooking stage during the nixtamalization process that was established when the moisture content in corn kernels reached a value of 36% (w/w) with a lime concentration of 1% (w/v), independent of the cooking temperature. Atomic absorption spectroscopy was used to determine the calcium concentration in the whole kernel and in its different anatomical components (pericarp, endosperm, and germ) as well as in 10% of the outermost layers, the next 10%, and the remaining 80% of the endosperm as a function of the steeping time. It was found that if the cooking temperature increases, the calcium content increases also. For steeping times in the range of 5–7 hr, a relative maximum was found in the calcium contents of 0.24, 0.21, and 0.18% (w/w) in QPM H-368 flours at 92, 82, and 72°C, respectively. Calcium was found in the most external layers in the endosperm and minimum diffusion occurs in the internal 80%. Phosphorous was measured by using UV spectroscopy and the results showed that it remains constant at 0.24% throughout the process. Scanning electron microscopy analysis was used to explain the calcium ion diffusion in the kernel. The physical changes in the pericarp govern the calcium diffusion process.  相似文献   

A human genome diversity cell line panel     

Cann HM  de Toma C  Cazes L  Legrand MF  Morel V  Piouffre L  Bodmer J  Bodmer WF  Bonne-Tamir B  Cambon-Thomsen A  Chen Z  Chu J  Carcassi C  Contu L  Du R  Excoffier L  Ferrara GB  Friedlaender JS  Groot H  Gurwitz D  Jenkins T  Herrera RJ  Huang X  Kidd J  Kidd KK  Langaney A  Lin AA  Mehdi SQ  Parham P  Piazza A  Pistillo MP  Qian Y  Shu Q  Xu J  Zhu S  Weber JL  Greely HT  Feldman MW  Thomas G  Dausset J  Cavalli-Sforza LL 《Science (New York, N.Y.)》2002,296(5566):261-262

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Isoform-specific modulation of Na+, K+-ATPase alpha-subunit gene expression in hypertension   总被引：6，自引：0，他引：6  

V L Herrera  A V Chobanian  N Ruiz-Opazo 《Science (New York, N.Y.)》1988,241(4862):221-223

Sodium, potassium-adenosine triphosphatase (Na+, K+-ATPase) is hypothesized to be involved in systemic vascular hypertension through its effects on smooth muscle reactivity and myocardial contractility. By means of RNA blot analyses of cardiac, aortic, and skeletal muscle RNAs in two rat hypertensive models, Na+,K+-ATPase alpha-subunit messenger RNA isoforms (alpha 2 and alpha 3) were shown to be deinduced in response to increased intravascular pressure. The changes were observed after 48 hours or more of experimental hypertension. Under these conditions, there is coordinate induction of another alpha isoform (alpha 1) and of beta-subunit messenger RNAs, probably in response to alterations in sodium flux rather than to elevated blood pressure.  相似文献   

Effects on yield and nutrition of mycorrhizal and nodulated Pueraria phaseoloides exerted by P-solubilizing rhizobacteria     

M. Toro  R. Azcón  R. Herrera 《Biology and Fertility of Soils》1996,21(1-2):23-29

We studied the effect of bacteria involved in rock phosphate (four isolates), iron phosphate (two isolates), and aluminium phosphate (two isolates) solubilization, and two phytate-mineralizing bacteria in terms of their interaction with two Glomus spp. on Pueraria phaseoloides growth and nutrition. The plant —Rhizobium sp. — mucorrhiza symbiosis system may increase in yield and nutrition in association with specific rhizosphere bacteria that solubilize calcium, iron, and aluminium phosphates. No benefit from phytate-mineralizing bacteria was found under these experimental conditions. P. phaseloides growth responses were influenced in different ways by specific combinations of the selected bacteria and arbuscular mycorrhizal fungi. Considerable stimulation of nutrient uptake was observed with fungus-bacteria combinations of Azospirillum sp. 1, Bacillus sp. 1 or Enterobacter (spp. 1 or 2) associated with G. mosseae. The fact that Bacillus sp. 1, a calcium-phosphate solubilizing isolate, positively interacted with G. mosseae and negatively with G. fasciculatum is an indication of specific functional compatibility between the biotic components integrated in the system. From our results, the interactions between bacterial groups able to solubilize specific phosphate and mycorrhizal fungi cannot be interpreted as occurring only via P solubilization mechanisms since no generalized effect was obtained. Iron-phosphate solubilizing microorganisms were more active alone than in dual associations with Glomus sp., but the aluminium-phosphate dissolving isolates positively interacted in mycorrhizal plants. Further work is needed in this area in order to elucidate the mechanisms that affect rhizosphere microorganism interactions. G. mosseae was more effective but less infective than G. fasciculatum in most of the combined treatments.  相似文献   

Genotypic diversity of Salmonella ser. Abortusequi isolates from Argentina     

C. P. Bustos  M. Moroni  M. I. Caffer  A. Ivanissevich  M. Herrera  A. R. Moreira  N. Guida  P. Chacana 《Equine veterinary journal》2020,52(1):98-103

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