PCR-based diagnosis for detection of Leishmania in skin and blood of rodents from an endemic area of cutaneous and visceral leishmaniasis in Brazil     

Oliveira FS  Pirmez C  Pires MQ  Brazil RP  Pacheco RS 《Veterinary parasitology》2005,129(3-4):219-227

The technique of polymerase chain reaction (PCR) associated to hybridization was used to screen 123 samples collected from wild and synanthropic rodents captured in a cutaneous and visceral leishmaniasis endemic area in the State of Minas Gerais, Brazil. The detection of Leishmania spp in naturally infected rodents is of fundamental importance for incriminating them as possible reservoir hosts of the diseases in Minas Gerais. A total of 62 specimens belonging to wild (Thrichomys apereoides, Oryzomys subflavus, Galea spixii, Bolomys lasiurus and Wiedomys pyrrhorhinos) and synanthropic (R. rattus) rodent species were captured in different ecotopes. Blood and skin samples were submitted for PCR analyses followed by molecular hybridization with specific probes for the three Leishmania-species complexes. Fifteen samples were found positive after PCR-hybridization and identified as follows: nine belonging to the L. mexicana complex, three to the L. braziliensis complex and three to the L. donovani complex. Positive PCR results were found in 11 out of the 61 (18%) blood samples and in four out of the 62 (6.4%) skin fragments screened. R. rattus and T. apereoides were the most abundant species in the area also presenting high prevalence of natural infection. The presence of parasite DNA belonging to L. braziliensis, L. mexicana and L. donovani complexes was confirmed in several individuals of a rodent species, R. rattus. This work is the first report of the detection of L. (L.) chagasi in a naturally infected T. apereoides. The utility of filter paper as a substrate for PCR analyses and the efficacy of the procedure associated to the hybridization is emphasized.  相似文献   

Scrapie genetic susceptibility in Portuguese sheep breeds     

Orge L  Galo A  Sepúlveda N  Simas JP  Pires M 《The Veterinary record》2003,153(16):508

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Transfer of Bovine Blastocysts Derived from Short-term In Vitro Culture of Low Quality Morulae Produced In Vivo     

RH Alvarez  M Meneghel  AC Martinez  RML Pires  EA Schammass 《Reproduction in domestic animals》2008,43(3):257-260

The aim of this study was to evaluate if blastocysts arising from in vitro culture of Grade 3 bovine morulae produced in vivo can promote acceptable pregnancy rates when transferred into recipients. Embryos of different stages and qualities were recovered from superovulated Bos taurus and B. indicus donors. Grade 3 morulae were cultured in either Holding Plus® or TCM‐199 (supplemented with 10% bovine fetal serum) media for 24 h at 38.5°C. After this culture period, the resulting blastocysts were morphologically classified (Grades 1, 2 and 3) and transferred into recipients previously synchronized with the donors. Non‐cultured Grades 1 and 3 morulae were used as control. Pregnancy diagnosis was carried out 60 days after embryo transfer and the data were analysed by logistic regression, considering variables, such as embryo quality (Grade), donor breed, culture medium, donor‐recipient synchrony and seasonality. Embryo quality was the only variable, showing significant effect on the pregnancy rate. Pregnancy rates for non‐cultured Grade 1 and 3 morulae, and blastocysts arising from cultured Grade 3 morulae were 58.1% (n = 31), 17.1% (n = 35) and 51.1% (n = 47), respectively (p < 0.05). There were no statistical differences between non‐cultured Grade 1 morulae and cultured blastocysts. Pregnancy rates for Grades 1 (65.0%) and 2 (60.0%) were higher than Grade 3 (29.4%) cultured blastocysts (p < 0.05). It was concluded that short‐term in vitro culture is a very convenient method of identifying morphologically low quality morulae with higher chances of continuing development after the transfer into recipients.  相似文献   

Critical limits of the degree of compactness and soil penetration resistance for the soybean crop in N Brazil   总被引：1，自引：0，他引：1       下载免费PDF全文

Pedro Daniel De Oliveira  Michel Keisuke Sato  Herdjania Veras de Lima  Sueli Rodrigues  Alvaro Pires da Silva 《植物养料与土壤学杂志》2016,179(1):78-87

In agricultural fields soil compaction is a major cause of physical degradation. Degree of compactness (DC) is a useful parameter for characterizing compaction and the response of crops for different soils. The objectives of this study were: (1) to identify the critical DC and PR values for soybean [Glycine max (L.) Merrill] using plant growth variables and (2) to verify the relationship between DC and PR, and assess which parameter is recommended for the evaluation of soil compaction. The study was conducted in a greenhouse in a completely randomized factorial design of 4 textures × 5 compaction levels for sandy loam and sandy clay loam soils, and 3 compaction levels for the clayey and very clayey soils. Soil samples were collected from the surface of a Xantic Kandiudox from the NE region of the State of Pará, Brazil. The DC was calculated from the maximum bulk density obtained by the Proctor test, and the PR curve was determined in undisturbed samples equilibrated in different matric potentials. The growth and development of the soybean was favored in the DC range of 80 to 85%, regardless of soil texture. The critical degree of compactness for the growth of soybean was around 98% regardless of soil texture, while the critical values for penetration resistance at field capacity varied according to soil texture and bulk density and were 28.2, 5.6, 3.5, and 5.2 MPa for the sandy loam, sand clay loam, clayey and very clayey soils, respectively. The root length was the plant growth variable most susceptible to soil compaction. Change in soil penetration resistance was poorly related with change in degree of compactness showing that one parameter cannot be replaced by the other. Because PR is quickly determined in field and have a direct relationship with plant growth, for the soils evaluated in this study we recommend the use of soil PR to assess the state of soil compaction.  相似文献   

Surface Water Quality Assessment of Lis River Using Multivariate Statistical Methods     

Judite S. Vieira  José C. M. Pires  Fernando G. Martins  Vítor J. P. Vilar  Rui A. R. Boaventura  Cidália M. S. Botelho 《Water, air, and soil pollution》2012,223(9):5549-5561

This study presents the application of multivariate statistical tools for the evaluation of spatial variations and the interpretation of water quality data obtained in a monitoring program of Lis river basin surface water, Portugal. Twenty-seven physicochemical and microbiological parameters were determined in six water sampling campaigns at 16 monitoring sites during the period from September 2003 to November 2006. Correlation analysis, principal component analysis, and cluster analysis were performed to evaluate the main water pollution sources and to characterize the spatial distribution of water pollution profiles in river basin. The results achieved with the statistical methodologies led to distinguish natural and anthropogenic pollution sources. Additionally, monitoring sites with similar water pollution profile were identified, indicating that some monitoring locations can be changed to improve the spatial characterization of water quality in the river basin. CBO, CQO, P, and N were identified as significant variables affecting spatial variations, namely in the Lis river middle reach. Besides the identification of main pollution sources, the applied statistical tools were able to identify spatial patterns of water pollution in Lis river basin, which further helps in the reassessment of the number and location of monitoring sites.  相似文献   

Prevention strategies for field traffic-induced subsoil compaction: a review: Part 1. Machine/soil interactions     

L. Alakukku  P. Weisskopf  W. C. T. Chamen  F. G. J. Tijink  J. P. van der Linden  S. Pires  C. Sommer  G. Spoor 《Soil & Tillage Research》2003,73(1-2):145-160

Subsoil compaction is a severe problem mainly because its effects have been found to be long-lasting and difficult to correct. It is better to avoid subsoil compaction than to rely on alleviating the compacted structure afterwards. Before recommendations to avoid subsoil compaction can be given, the key variables and processes involved in the machinery–subsoil system must be known and understood. Field traffic-induced subsoil compaction is discussed to determine the variables important to the prevention of the compaction capability of running gear. Likewise, technical choices to minimise the risk of subsoil compaction are reviewed. According to analytical solutions and experimental results the stress in the soil under a loaded wheel decreases with depth. The risk of subsoil compaction is high when the exerted stresses are higher than the bearing capacity of the subsoil. Soil wetness decreases the bearing capacity of soil. The most serious sources of subsoil compaction are ploughing in the furrow and heavy wheel loads applied at high pressure in soft conditions. To prevent (sub)soil compaction, the machines and equipment used on the field in critical conditions should be adjusted to actual strength of the subsoil by controlling wheel/track loads and using low tyre inflation pressures. Recommendations based on quantitative guidelines for machine/soil interactions should be available for different wheel load/ground pressure combinations and soil conditions.  相似文献   

The effect of enzyme supplementation on the true ileal digestibility of a lupin based diet for piglets     

V.M.R. Pires  C.M.A. Fontes  L.M.A. Ferreira  C.I.P.D. Guerreiro  L.F. Cunha  J.P.B. Freire   《Livestock Science》2007,109(1-3):57-59

A trial involving 24 weaned piglets was conducted to evaluate the effect of two alpha-galactosidases, associated or not with a cellulase and hemicellulase enzyme mixtures, for improving the nutritive value of a cereal–Lupinus albus based diet. Enzyme supplementation was unable to improve apparent ileal digestibility of crude protein and true ileal digestibility of amino acids. However, ileal digestibility of NDF tended to increase with enzyme supplementation, suggesting a residual hemicellulase activity in both -galactosidases mixtures under analysis.  相似文献   

Associations of leptin gene polymorphisms with production traits in pigs   总被引：1，自引：0，他引：1  

J. de Oliveira Peixoto  S.E. Facioni Guimarães  P. Sávio Lopes  M.A. Menck Soares  A. Vieira Pires  M.V. Gualberto Barbosa  R. de Almeida Torres  & M. de Almeida e Silva 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(6):378-383

The associations of leptin (LEP) gene polymorphisms C798T, T2411C, T3266G and T3469C with production traits were investigated in a F2 pig population produced by divergent crosses. The statistical model included genotype, sex, batch and genotype by sex interaction as fixed effects and sire as random effect. Polymorphism C798T was associated with variation in total teat number (p < 0.02) and left teat number (p < 0.03), and polymorphism T3469C was associated with weight at 21 days (p < 0.03), 42 days (p < 0.05), 63 days (p < 0.02) and 77 days of age (p < 0.04) as well as feed intake (p < 0.01), average daily gain (p < 0.01), feed conversion (p < 0.01), bacon depth (p < 0.03) and slaughter weight (p < 0.03). Phenotypic associations were also performed by combining T3469C and C798T genotypes. Interaction between C798T genotypes and sex was observed for some traits. LEP genotypes had significant influence on performance traits, and can be considered as potential genetic markers for selection. However, these results have to be validated in commercial herds.  相似文献   

Effects of surface‐applied and soil‐incorporated lime on some physical attributes of a Dystrudept soil          下载免费PDF全文

A. C. Auler  L. F. Pires  J. A. B. dos Santos  E. F. Caires  J. A. R. Borges  N. F. B. Giarola 《Soil Use and Management》2017,33(1):129-140

It is generally accepted that liming ameliorates soil acidity. However, the method of lime application is thought by many to influence its effectiveness in acid soils. In this study, we wanted to assess the degree of effectiveness of surface‐applied lime and lime incorporated into the soil on soil structural attributes and water retention of a Dystrudept soil in the SE region of the State of Paraná, Brazil. Lime was added at the rate of 15 t/ha to soil through: (i) surface broadcasting, (ii) incorporation via ploughing and harrowing, (iii) incorporation via subsoiling and harrowing. A control treatment with zero lime application was included in the experiment. The addition of lime by surface broadcasting resulted in significant reductions in soil bulk density (BD) and macroporosity (Ma) and increases in total porosity (TP) and microporosity (Mi) of the top soil layer (0–0.10 m). The reverse was the case in the 0.10‐ to 0.20‐m soil layer; where lime was incorporated via ploughing and harrowing, increases in BD and reductions in TP and Ma were observed. Addition of lime also significantly increased soil water retention, with maximum retention recorded from soil amended with surface broadcast lime. Changes in soil chemical attributes (increases in pH, Ca²⁺ and Mg²⁺ contents; reductions in potential acidity and Al³⁺ content) were responsible for the changes observed in structural and physical attributes, and water retention. Bearing in mind the lower application costs, improvement in the soil chemical attributes for plant development and soil physical quality, surface broadcast lime can be considered a promising alternative for no‐till farmers.  相似文献   

The role of integrating agroforestry and vegetable planting in structuring communities of herbivorous insects and their natural enemies in the Neotropical region     

Érica Sevilha Harterreiten-Souza  Pedro Henrique Brum Togni  Carmen Silvia Soares Pires  Edison Ryoiti Sujii 《Agroforestry Systems》2014,88(2):205-219

The integration of agroforestry plots with ephemeral crops such as vegetables on the farm scale can probably serve as a refuge and source of beneficial insects. Therefore, agroforestry systems possibly represent an alternative that favors ecosystem services and help growers in the transition process from conventional to agro-ecological agriculture. This study aimed to understand the role of introducing agroforestry systems in structuring insect communities, with consequences for the abundance of herbivore and natural enemies, contributing to farm management and favoring biological control as an ecosystem service. Field surveys showed that agroforestry systems can harbor more species than horticultural crops, independent of the functional group. They also contain a greater diversity of herbivores and an even greater diversity profile of natural enemy communities. Agroforestry systems served as a source of natural enemies that can colonize horticultural crops when herbivores are present. As a consequence, natural enemies can establish a numerical response to herbivore abundance, but their communities are also affected by stochastic factors related to climatic conditions. Thus, agroforestry systems and agro-ecological practices might favor an agriculture based on the maintenance and conservation of ecosystem services.  相似文献