Detection of pseudocowpox virus in water buffalo (Bubalus bubalis) with vesicular disease in the state of São Paulo,Brazil, in 2016     

Mateus Laguardia-Nascimento  Ana Paula Ferreira de Oliveira  Fernanda Rodas Pires Fernandes  Anselmo Vasconcelos Rivetti Junior  Marcelo Fernandes Camargos 《The Veterinary quarterly》2017,37(1):16-22

Background: Parapoxviruses are zoonotic viruses that infect cattle, goats and sheep; there have also been reports of infections in camels, domestic cats and seals.

Objective: The objective of this report was to describe a case of vesicular disease caused by pseudocowpox virus (PCPV) in water buffalo (Bubalus bubalis) in Brazil.

Animals: Sixty buffalo less than 6 months old exhibited ulcers and widespread peeling of the tongue epithelium. There were no cases of vesicular disease in pigs or horses on the same property.

Methods: Samples were analysed by PCR and sequencing. Phylogenetic analysis in MEGA 7.01 was reconstructed using major envelope protein (B2L) by the Tamura three-parameter nucleotide substitution model and the maximum likelihood and neighbor joining models, both with 1000 bootstrap replicates. The genetic distance between the groups was analysed in MEGA using the maximum composite likelihood model. The rate variation among sites was modeled using gamma distribution.

Results: The presence of PCPV in the buffalo herd could be demonstrated in epithelium and serum. The minimum genetic distance between the isolated PCPV strain (262-2016) and orf virus and bovine papular stomatitis virus was 6.7% and 18.4%, respectively. The maximum genetic distance calculated was 4.6% when compared with a PCPV detected in a camel.

Conclusions/Clinical Importance: The peculiar position of the isolated strain in the phylogenetic trees does not necessarily indicate a different kind of PCPV that infects buffalo. More samples from cattle and buffalo in Brazil must be sequenced and compared to verify if PCPV from buffalo are genetically different from samples derived from cattle.  相似文献   

Within- and between-tree variation of wood density components in <Emphasis Type="Italic">Pinus nigra</Emphasis> at six sites in Portugal     

Alexandra Dias  Maria J. Gaspar  Ana Carvalho  Jani Pires  José Lima-Brito  Maria E. Silva  José L. Louzada 《Annals of Forest Science》2018,75(2):58

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Effects of inbreeding on population mean performance and observational variances in <Emphasis Type="Italic">Eucalyptus globulus</Emphasis>     

João Costa e Silva  Craig Hardner  Paul Tilyard  Ana M. Pires  Brad M. Potts 《Annals of Forest Science》2010,67(6):605-605

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Consequences of Nitric Oxide Synthase Inhibition During Bovine Oocyte Maturation on Meiosis and Embryo Development     

KRL Schwarz  PRL Pires  THC de Bem  PR Adona  CLV Leal 《Reproduction in domestic animals》2010,45(1):75-80

The importance of nitric oxide synthase (NOS) in bovine oocyte maturation was investigated. Oocytes were in vitro matured with the NOS inhibitor N^w- l -nitro-arginine methyl-ester (10⁻⁷, 10⁻⁵ and 10⁻³  m l -NAME) and metaphase II (MII) rates and embryo development and quality were assessed. The effect of l -NAME (10⁻⁷  m ) during pre-maturation and/or maturation on embryo development and quality was also assessed. l -NAME decreased MII rates (78–82%, p < 0.05) when compared with controls without l -NAME (96%). Cleavage (77–88%, p > 0.05), Day 7 blastocyst rates (34–42%, p > 0.05) and total cell numbers in blastocysts were similar for all groups (146–171 cells, p > 0.05). Day 8 blastocyst TUNEL positive cells (3–4 cells) increased with l -NAME treatment (p < 0.05). For oocytes cultured with l -NAME during pre-maturation and/or maturation, Day 8 blastocyst development (26–34%) and Day 9 hatching rates (15–22%) were similar (p > 0.05) to controls pre-matured and matured without NOS inhibition (33 and 18%, respectively), while total cell numbers (Day 9 hatched blastocysts) increased (264–324 cells, p < 0.05) when compared with the controls (191 cells). TUNEL positive cells increased when NOS was inhibited only during the maturation period (8 cells, p < 0.05) when compared with the other groups (3–4 cells). NO may be involved in meiosis progression to MII and its deficiency during maturation increases apoptosis in embryos produced in vitro . Nitric oxide synthase inhibition during pre-maturation and/or maturation affects embryo quality.  相似文献   

Histopathologic and Immunohistochemical Exam in One Case of Canine Endometrial Adenocarcinoma     

MA Pires  F Seixas  C Palmeira  R Payan‐Carreira 《Reproduction in domestic animals》2010,45(3):545-549

Canine endometrial carcinomas are rare, and mostly occur in geriatric bitches. In this work, the uterus of a 10‐year‐old female Boxer evidencing an endometrial carcinoma on the body of the uterus was used to describe the histopathological features of the tumour and to study its immunophenotype. In this work, a panel of immunomarkers (cytokeratins AE1/AE3 and 14, vimentin, CD10 and Ki‐67) was applied to the endometrial carcinoma to establish the staining patterns indicative of the tumour agressiveness and cellular differentiation. Additionally DNA ploidy was also performed. In this case, the tumour showed papillar pattern, with large pleomorphic, anaplastic cells and also some aberrant multinucleated and giant cells. In some areas of the tumour, it was also observed cytotrophoblastic‐like cells outlining the papillae. Cytokeratin AE1/AE3 expression was detected in the luminal neoplasic cells. Cytokeratin 14 positivity was sporadic and irregular, and was observed mainly in the luminal epithelium. Only stromal and aberrant cells showed a positive staining to vimentin. Positive membranous staining to CD10 was evidenced by clear epithelial, cytotrophoblastic‐like cells at the tumour surface but not by the stromal cells. The mitotic and Ki‐67 indices were low, suggestive of a weak aggressiveness of the tumour. The multinucleated and giant cells evidenced a positive immunostaining to CK AE1/AE3, and CD 10; its positivity to vimentin was sporadic. This study aims to contribute to the advancement of the knowledge in canine endometrial carcinoma immunophenotype.  相似文献   

<Emphasis Type="Italic">Trichospilus diatraeae</Emphasis> Cherian &; Margabandhu, 1942 (Hymenoptera: Eulophidae), a new parasitoidof <Emphasis Type="Italic">Melanolophia consimilaria</Emphasis> (Lepidoptera: Geometridae)     

Bruno Zaché  Carlos Frederico Wilcken  Ronelza Rodrigues Dacosta  Everton Pires Soliman 《Phytoparasitica》2010,38(4):355-357

First report on the parasitoid Trichospilus diatraeae of the genus in Eulophidae found in the field parasitizing pupae of the eucalyptus defoliating looper Melanolophia consimilaria. This opens new perspectives for the use of this parasitoid in biological control programs against caterpillars of forestry importance.  相似文献   

Micromorphological analysis to characterize structure modifications of soil samples submitted to wetting and drying cycles     

L.F. Pires  M. Cooper  F.A.M. Cássaro  K. Reichardt  O.O.S. Bacchi  N.M.P. Dias 《CATENA》2008

The physical characteristics of the soil surface are of extreme importance in relation to energy and matter transfer processes between the atmosphere and the soil. Soil internal structure changes can be due to natural or artificial causes and one important natural process is the alternation of wetting and drying (W–D) processes, which induce swelling and shrinking of soil particles, causing modifications in pore size and shape. To study the consequence of these W–D events on possible modifications in pore size distribution, pore number, and pore shape of soil samples collected in metal rings pore image analysis was used. Samples were taken from profiles of three soils of different characteristics, named as Geric Ferralsol (GF), Eutric Nitosol (EN), and Rhodic Ferralsol (RF). Confined volumetric samples (50 cm³) were submitted to none (T₀), three (T₁), and nine (T₂) subsequent W–D cycles. Image cross sections of resin impregnated soil permitted the micrometric and macrometric characterization of changes in soil structure induced by sequences of W–D cycles. Duncan's statistical test indicated that there were significant differences (α = 0.05) among treatments for all soil samples. General conclusions indicate that total pore area increased for all soils after repeated W–D processes, specifically 19.0 to 28.9% for GF, 5.9 to 11.7% for EN, and 13.0 to 17.2% for RF. Main changes of pore diameter occurred in pores larger than 500 μm, and minor changes were observed in the total number of these pores. It is demonstrated that soil samples undergo important changes in their structures after repeated W–D cycles. The information presented here is very important for the evaluation of soil water retention curves and other soil hydric properties, because soil samples used in these procedures are collected in rings and frequently submitted to several W–D cycles.  相似文献   

Phytotoxity of Fusarium subglutinans culture filtrates on in vitro plantlets and calli of resistant and susceptible pineapple (Ananas comosus)     

Hidalgo  Santos  Tussel  Pires de Matos  Cabral  Arzola  & Pérez 《Plant pathology》1999,48(6):756-758

Various concentrations of culture filtrates from Fusarium subglutinans were tested for their effect on in vitro multiplication of plantlets and regeneration from calli of pineapple. The cultivars tested were the resistant Perolera and Spring and the susceptible Perola and Smooth Cayenne. The culture filtrate reduced shoot formation of in vitro plantlets and regeneration of plants from calli derived from the susceptible cultivars but plantlets and calli from resistant plants showed resistance. These results show the potential for culture filtrates of F. subglutinans to provide a method of in vitro screening of pineapple for resistance to the pathogen.  相似文献   

Prevention strategies for field traffic-induced subsoil compaction: a review: Part 1. Machine/soil interactions     

L. Alakukku  P. Weisskopf  W. C. T. Chamen  F. G. J. Tijink  J. P. van der Linden  S. Pires  C. Sommer  G. Spoor 《Soil & Tillage Research》2003,73(1-2):145-160

Subsoil compaction is a severe problem mainly because its effects have been found to be long-lasting and difficult to correct. It is better to avoid subsoil compaction than to rely on alleviating the compacted structure afterwards. Before recommendations to avoid subsoil compaction can be given, the key variables and processes involved in the machinery–subsoil system must be known and understood. Field traffic-induced subsoil compaction is discussed to determine the variables important to the prevention of the compaction capability of running gear. Likewise, technical choices to minimise the risk of subsoil compaction are reviewed. According to analytical solutions and experimental results the stress in the soil under a loaded wheel decreases with depth. The risk of subsoil compaction is high when the exerted stresses are higher than the bearing capacity of the subsoil. Soil wetness decreases the bearing capacity of soil. The most serious sources of subsoil compaction are ploughing in the furrow and heavy wheel loads applied at high pressure in soft conditions. To prevent (sub)soil compaction, the machines and equipment used on the field in critical conditions should be adjusted to actual strength of the subsoil by controlling wheel/track loads and using low tyre inflation pressures. Recommendations based on quantitative guidelines for machine/soil interactions should be available for different wheel load/ground pressure combinations and soil conditions.  相似文献   

Critical limits of the degree of compactness and soil penetration resistance for the soybean crop in N Brazil   总被引：1，自引：0，他引：1       下载免费PDF全文

Pedro Daniel De Oliveira  Michel Keisuke Sato  Herdjania Veras de Lima  Sueli Rodrigues  Alvaro Pires da Silva 《植物养料与土壤学杂志》2016,179(1):78-87

In agricultural fields soil compaction is a major cause of physical degradation. Degree of compactness (DC) is a useful parameter for characterizing compaction and the response of crops for different soils. The objectives of this study were: (1) to identify the critical DC and PR values for soybean [Glycine max (L.) Merrill] using plant growth variables and (2) to verify the relationship between DC and PR, and assess which parameter is recommended for the evaluation of soil compaction. The study was conducted in a greenhouse in a completely randomized factorial design of 4 textures × 5 compaction levels for sandy loam and sandy clay loam soils, and 3 compaction levels for the clayey and very clayey soils. Soil samples were collected from the surface of a Xantic Kandiudox from the NE region of the State of Pará, Brazil. The DC was calculated from the maximum bulk density obtained by the Proctor test, and the PR curve was determined in undisturbed samples equilibrated in different matric potentials. The growth and development of the soybean was favored in the DC range of 80 to 85%, regardless of soil texture. The critical degree of compactness for the growth of soybean was around 98% regardless of soil texture, while the critical values for penetration resistance at field capacity varied according to soil texture and bulk density and were 28.2, 5.6, 3.5, and 5.2 MPa for the sandy loam, sand clay loam, clayey and very clayey soils, respectively. The root length was the plant growth variable most susceptible to soil compaction. Change in soil penetration resistance was poorly related with change in degree of compactness showing that one parameter cannot be replaced by the other. Because PR is quickly determined in field and have a direct relationship with plant growth, for the soils evaluated in this study we recommend the use of soil PR to assess the state of soil compaction.  相似文献