Improved Assembly of the Hartung-Clark Double Cannula for the Isolated Frog Heart     

Lu G 《Science (New York, N.Y.)》1948,107(2775):255-256

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东北地区外来针叶树种引种现状及发展策略   总被引：2，自引：0，他引：2  

陆志民  章林  赵瑛  窦万军  于洪民  王琦  金日录  金点仙 《东北林业大学学报》2004,32(5):23-25

介绍了东北林区主要针叶树种引种现状及取得的成就，分析了东北三省针叶树种引种工作中存在的问题，提出了建立较为科学的引种网络体系和发展策略，并提供了可能在干旱、半干旱区引种成功的针叶树种名  相似文献   

Acid-induced autoagglutination found in chicken pathogenic Escherichia coli strain.     

T Sekizaki  Y Nakasato  I Nonomura 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(3):493-499

Chicken pathogenic Escherichia coli strains were found to autoagglutinate in a static culture of trypticase soy broth (TSB). One strain, designated PDI-386, was further studied for its autoagglutinating property. Acidity in the cultured medium caused by glucose degradation induced the autoagglutination. The bacterial cells grown in a glucose-free L-broth could be aggregated by adding acid, which suggests a potentiality of autoagglutination of the strain grown in the L-broth. The autoagglutinating parent (Agg) formed small colonies with irregular edges like rough colonies on the TS agar, whereas its non-autoagglutinating variant (Nag) formed larger smooth colonies with a perfectly round edge. The Nag colony was easily generated from the Agg colony on the TS agar. The autoagglutinating property was very unstable when the bacteria was passed in the TSB, but rather stable in the L-broth. Under electron microscope, the Agg were found to possess pili of more than 20 microns in length. However, the phenotypic expression of autoagglutination did not correlate with that of mannose-sensitive hemagglutination against guinea pig erythrocytes. Incubation of the Nag in the L-broth at room temperature for more than 10 days provoked the reversion of the autoagglutination. There was no difference between the Agg and the Nag in terms of surface hydrophobicity, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns of membrane proteins and LPS, and plasmid profiles. The virulence of the Agg was higher than that of the Nag. The autoagglutination property is, however, so unstable that the pathogenicity of E. coli isolates from chickens should be carefully evaluated.  相似文献   

Characterization of phosphate species in urban sewage sludges by high-resolution solid-state ³¹P NMR     

E. FROSSARD  P. TEKELY  J.Y. GRIMAL 《European Journal of Soil Science》1994,45(4):403-408

The mineral forms of phosphorus in three urban sewage sludges were characterized using high-resolution solid-state phosphorus-31 nuclear magnetic resonance (NMR) coupled to a sequential extraction. The sludges studied were an anaerobically-digested and heat-treated sludge (Paris-Achères), an activated sludge (Briare) and an anaerobically-digested sludge (Nancy). NMR observations were conducted using both single-pulse and cross-polarization sequences in order to distinguish between ³¹P nuclei far from ¹H nuclei, and ³¹P located within a fraction of a nanometre of ¹H. This approach showed that a complex mixture of P species was present in these sludges. A mixture of hydrogenated octocalcium phosphates and apatites was observed in the three samples. Monetite was present in the anaerobically-digested sludge and brushite in the activated sludge. Dehydrogenated condensed calcium phosphates (compounds with a Ca:P ratio higher than 1.0 such as fluorapatite or tricalcium phosphate) and dehydrogenated pyrophosphates were also probably present in the anaerobically-digested sludge. A poorly-ordered wavellite was observed in the three sludges after the HCl extraction. However, results were inconclusive as to whether this mineral was present in the three sludges, or had been precipitated during the sequential extraction.  相似文献   

杉木优良种源在苏南低山丘陵地区的推广应用     

/Lu xiangsheng et al. 《江苏林业科技》1996,23(2):10-13

本文报道了杉木优良源在江苏南部低山丘陵地区推广的背景和依据，组织体系，措施与配套技术，推广情况及其经济，生态，社会效益。  相似文献   

犬瘟热的微生物学诊断研究   总被引：13，自引：1，他引：12  

房红莹  陆承平 《中国兽医学报》1997,17(2):148-151

对临床诊断为犬瘟热的１４例病犬，取其脑组织，运用细胞培养、合胞体检查、包涵体检查、免疫荧光试验、电镜观察等５种检测方法，就犬瘟热的微生物学诊断进行了系统的研究。结果表明，犬脑组织块与猫胚（ＦＥ）细胞或非洲绿猴肾（Ｖｅｒｏ）细胞共同培养，盲传的培养物出现不稳定的细胞病变，通过对不同代次的培养物检查包涵体，并作超薄切片或负染，电镜观察犬瘟热病毒（ＣＤＶ）粒子，证实分离到ＣＤＶ野毒。病犬脑组织切片经ＨＥ染色检查包涵体及用ＣＤＶ荧光抗体直接法检测脑抹片及接种犬脑的细胞培养物，均获得一定的阳性结果。建立的改良离子捕获电镜法，用于检测犬脑匀浆和犬脑接种细胞培养物，与离子捕获电镜法、免疫电镜法及直接电镜法相比，效果更为理想。１４例临床诊断为犬瘟热的病犬，综合运用上述微生物学手段检测，结果为１２例阳性，２例疑似。  相似文献   

用紫外分光法测定鱼藤中异黄酮类的总含量     

陈欣安  陆磊 《中南林业科技大学学报(自然科学版)》1996,(1)

根据鱼藤中异黄酮类化合物在紫外光２１０～２４５ｎｍ及２９０～３０５ｎｍ处具有吸收峰这一特性，提出了用紫外分光法测定鱼藤中异黄酮类总含量的方法．在３００ｎｍ波长处，浓度低于６×２．６１８×１０－３／ｍｏｌ·Ｌ－１时，测定了标准系列的吸光值，结果表明吸光值与浓度近乎直线相关，相关系数为０．９９７７；标准系列吸光值准确性高；加样回收率达９９％以上；测定样品时，重现性好．  相似文献   

茶树种质资源抗茶云纹叶枯病鉴定   总被引：3，自引：0，他引：3  

洪北边  楼云芬 《植物病理学报》1996,26(3):215-216

 茶云纹叶枯病(Guignardia camelliae,无性时代为Colletotrichum camelliae)在我国各产茶省(区)均有发生和为害。对该病的发生规律及防治研究有过报导,但对茶树种质资源的抗病性研究报导甚少,本文简要报道30份茶种质资源抗茶云纹叶枯病的鉴定试验研究结果。  相似文献   

Comparative effects of mu and kappa opiate agonists on the cecocolic motility in the pony.          下载免费PDF全文

T Roger  T Bardon    Y Ruckebusch 《Canadian journal of veterinary research》1994,58(3):163-166

The electrical and mechanical activity of the large intestine and its response to administration of opiate mu and kappa agonists were assessed from electrodes and inductograph coils chronically implanted on the cecocolic segment in six ponies given a diet of hay and concentrates. Before the drugs were given, migrating complexes propagating from the cecum into the colon occurred at the rate of 1.5 to 16/hour. During this propulsive activity, the cecocolic sphincter opened and closed allowing the outflow of cecal contents and preventing the backflow of colic contents. Each pony was used as its own control and was given fentanyl (0.01 and 0.05 mg/kg of body weight, IV) and U50488H (0.1 and 0.5 mg/kg, IV) at weekly intervals. The mu agonist fentanyl elicited a marked phase of inhibition of the propulsive activity and a closure of the cecocolic sphincter that lasted one to two hours depending on the dose. The kappa agonist U50488H induced an inhibition of the short spiking activity, i.e. of the resting muscle tone. It did not disturb the occurrence of migrating complexes nor that of the openings of the cecocolic sphincter. These kappa compounds may be drugs of choice to alleviate visceral pain in colic stases without inducing delay of transit unlike mu compounds.  相似文献   

钾肥施用量对脐橙产量和品质的影响   总被引：30，自引：0，他引：30  

鲁剑巍  陈防  万运帆  刘冬碧  余常兵  王耀群  宋发安  王运华  Rolf Hardter 《果树学报》2001,18(5):272-275

１９９６～１９９９年连续４年在湖北省秭归县进行了脐橙钾肥施用量田间试验。结果表明，尽管各处理脐橙产量年度变化很大，但施钾显著提高产量和经济效益，１９９７～１９９９年施钾平均增产２６．３％～４１．８％，施钾（Ｋ２Ｏ）１２５、２５０、３７５ｋｇ／ｈｍ２年均增加纯利 １０１１０、１５８４０、９９７０元／ｈｍ２，产投比分别为 １９．３８、１５．４０和７．０４；在试验条件下施钾（Ｋ２Ｏ）２５０ｋｇ／ｈｍ２增产效果最好；施钾有提高脐橙外观品质和内在品质的趋势，例如施钾提高或保持单果重、增加果皮厚度、提高维生素Ｃ含量等；但施钾对品质的影响年度间差异较大，尚需进一步研究。  相似文献