Structural characterization of carangid fish myoglobins     

Hasan MM  Watabe S  Ochiai Y 《Fish physiology and biochemistry》2012,38(5):1311-1322

The primary structures of myoglobin (Mb) from the following five carangid species were determined: yellowtail Seriola quinqueradiata, greater amberjack Seriola dumerili, yellowtail kingfish Seriola lalandi, Japanese horse mackerel Trachurus japonicus, and silver trevally Pseudocaranx dentex. The sequences were of varying composition both in the coding and in the noncoding regions, but all contained the open reading frame of 444 nucleotides encoding 147 amino acids. Amino acid sequence identities of carangid Mbs were in the range of 81-99%. The similarity of the heme pocket and associated heme-binding residues of carangid Mbs were evidence of the conservative nature of Mbs. Similar to the other teleost Mbs, carangid Mbs did not contain a D helix and had mostly conserved A and E helices as well as E-F and G-H inter-helical segments. Hydropathy profiles of carangid Mbs showed species-specific variations where silver trevally Mb exhibited generally higher hydrophobicity. Phylogenetic analysis based on the primary structures was in agreement with conventional morphological taxonomy, establishing close proximity of carangid Mbs with those of cichlid and scombroid, the other members of the Perciformes order.  相似文献   

Use of a hand-held meter for detecting subclinical ketosis in dairy cows     

Huseyin Voyvoda  Hasan Erdogan 《Research in veterinary science》2010,89(3):344-351

The Optium Xceed is a new hand-held meter for determining blood β-hydroxybutyrate (BHBA) and glucose in human medicine. The objective of this study was to compare BHBA and glucose results obtained using the hand-held meter with those results made with a laboratory method and to evaluate its usefulness as a cowside test in the diagnosis of subclinical ketosis (SCK) in dairy cows. Seventy-eight blood samples from clinically healthy Holstein cows between 5 and 60 days post-calving were analysed. BHBA and glucose values were significantly higher with the hand-held meter versus laboratory methods. Correlation coefficients (r) for BHBA and glucose with the Optium Xceed versus laboratory methods were 0.97 and 0.63, respectively. Based on Bland–Altman plot and Passing–Bablok regression, agreement between two methods was good for BHBA but the agreement for glucose was only fair. When SCK was defined as plasma BHBA levels 1200 μmol/L, the sensitivity and specificity of the hand-held meter ketone testing in determining SCK were 85% and 94%, respectively. Raising the threshold of the laboratory method to 1400 μmol/L, the sensitivity and specificity incremented to 0.90 and 0.98, respectively.In conclusion, the blood ketone-monitoring device can be used as a rapid and reliable diagnostic test to detect SCK under field conditions.  相似文献   

Reproductive performance of Holstein dairy cows in Iran   总被引：2，自引：2，他引：0  

Maryam Ansari-Lari  Mojtaba Kafi  Mohammad Sokhtanlo  Hasan Nategh Ahmadi 《Tropical animal health and production》2010,42(6):1277-1283

The objectives of the present study were to describe the current reproductive parameters of Holstein dairy cows in Iran and to determine factors which have a significant effect on reproductive performance with emphasis on high-producing dairy cows. Five large Holstein dairy herds from the major milk production areas of Iran were selected for this study. Reproduction data including parity of dam, calving date, days to first service, days open, number of services, dry period, and occurrence of diseases for cows that calved from 2004 to 2007 were collected from the herds' databases. To determine the effects of year and season of calving, disease status, level of milk production, and parity of dam on reproductive parameters, the proc-mixed procedure in SAS software was used. Overall, data about 8,204 lactation were collected for the study. Results showed that mean (±SD) days open, calving interval, and days to first service for study herds were 134 (±89), 403 (±86), and 67 (±38) days, respectively. Conception rates at the first service and the overall service-conception rate were 41.6% and 41%, respectively. The level of milk production and diseases had significant negative effects on days open and service per conception (P < 0.05); statistical analysis showed that for every 100-kg increase in milk yield, days open will increase by about 0.3 days. However, no significant effect of level of productivity was observed on days to first service. The results of this study indicate that high milk production is a risk factor for decreasing fertility in Iran, like many other parts of the world.  相似文献   

Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk     

Onur Atakisi  Hasan Oral  Oguz Merhan  Ayla Ozcan  Bulent Polat  Semra Kaya 《Research in veterinary science》2010,89(1):10-13

The aim of this study was to investigate total antioxidant (TAC), and oxidant capacity (TOC) and nitric oxide (NO) levels in milk of cows with subclinical mastitis. Brown Swiss and Holstein breed cows were screened with California Mastitis Test (CMT) to determine mammary glands with subclinical mastitis. Moreover, somatic cell counts (SCC) were determined electronically in all milk samples. Mammary quarters were classified as healthy (n = 25) or subclinical mastitis (n = 35) based on CMT scores and somatic cell count (SCC: ?200,000/ml or >200,000/ml) in milk. Nitric oxide, TOC and SCC levels were significantly higher (p < 0.001, p < 0.005 and p < 0.001, respectively) in milk from mammary quarters with subclinical mastitis compared to those from healthy mammary quarters. In conclusion, subclinical mastitis results in higher NO concentrations, TOC and SCC, and NO and TOC were positively correlated with SCC. Moreover, alterations in NO levels and TOC in milk could be used as an alternative diagnostic tool to screen for subclinical mastitis.  相似文献   

Evaluation of radiolabeled and colorimetric DNA probes in comparison with an antigen screening assay for the detection of Salmonella from poultry farms     

J A Hasan  I T Knight  C R Tate  E T Mallinson  R G Miller  R R Colwell  S W Joseph 《Avian diseases》1991,35(2):397-402

A total of 48 environmental drag-swab samples from various poultry farms were tested for the presence of Salmonella spp. by culture, an enzyme-linked immunosorbent assay-based Salmonella antigen screening (SAS) assay, and two DNA probes (radiolabeled and colorimetric). The radiolabeled DNA probe was allowed to hybridize with culture-positive samples (n = 8) and was found to detect Salmonella spp. in all cases (100%). Both of the probes, subsequently hybridized with culture-negative samples (n = 8), were observed to yield good agreement (91%) with the culture findings. The remaining samples (n = 32) were tested by the SAS assay, and where there was no agreement between the culture and SAS, samples were further examined by the DNA probes. Results using both probes agreed with those obtained by culturing the samples but did not agree with the SAS assay result when the ratio of samples tested to samples positive (S/P) cutoff value used was 0.5.  相似文献   

Trends in the frequency of calcium oxalate uroliths in the upper urinary tract of cats     

Lekcharoensuk C  Osborne CA  Lulich JP  Albasan H  Ulrich LK  Koehler LA  Carpenter KA  Swanson LL  Pederson LA 《Journal of the American Animal Hospital Association》2005,41(1):39-46

Medical records from cats diagnosed with uroliths at nine United States veterinary teaching hospitals from 1980 to 1999, and records of cats with uroliths submitted for analyses to the Minnesota Urolith Center from 1981 to 2000, were evaluated. A 10-fold increase in frequency of upper tract uroliths occurred in cats during the 20-year interval at the nine veterinary teaching hospitals. Calcium oxalate emerged as the predominant mineral type in upper tract uroliths, having increased more than 50-fold during the study period. These results emphasize the need for increased awareness of the occurrence of upper urinary tract uroliths in cats.  相似文献   

Use of doramectin for treatment of sarcoptic mange in five Angora rabbits     

Voyvoda H  Ulutas B  Eren H  Karagenc T  Bayramli G 《Veterinary dermatology》2005,16(4):285-288

The efficacy of administering doramectin after moxidectin treatment, which has previously proved only partially effective, was evaluated in five Angora rabbits naturally infested with Sarcoptes scabiei mange. Evaluations included physical examination for clinical signs of sarcoptic mange and collection of skin scrapings for determination of mites. The rabbits first received two subcutaneous injections, 10 days apart, of moxidectin 1% injectable solution at a dosage of 0.2 mg kg(-1) of bodyweight. Although moxidectin treatment resulted in clinical improvement within 10 days post initial injection, on days 10 and 35 post initial treatment live mites were present in skin scrapings. Administration of doramectin 1% injectable solution using the same route and dosage and at similar intervals to moxidectin led to complete disappearance of signs of scabies and parasitological cure in all rabbits.  相似文献   

番茄细菌性溃疡病苗期接种新方法的研究   总被引：5，自引：0，他引：5  

罗来鑫  李健强  Hasan BOLKAN 《植物病理学报》2005,35(2):123-128

 在剪叶、浸根和针刺等细菌病害传统接种方法基础上设计了打顶接种新方法;以番茄细菌性溃疡病菌中国菌株和美国菌株借助打顶法接种佳粉10、合作908和华南红宝石3个国内主栽番茄品种2~3片真叶期幼苗,在昼夜最低、最高温度15~18℃、32~35℃和相对湿度为30%~60%的温室条件下,打顶法接种番茄幼苗后溃疡病发病率为87.5%~100.0%,病情指数随接种菌悬液浓度提高而增大,达到23.96~82.29,3个供试品种之间表现稳定一致;剪叶、浸根和针刺接种方法的发病率普遍在40%以下,病情指数在20以下,3个供试品种之间未表现明显差异;进一步研究显示,使用选择性培养基mSCM能够从打顶法接种后的发病植株上获得培养性状与原接种体一致的分离物,专化性免疫凝聚试剂盒检测到特定的测试线,PCR扩增到614bp的特异性条带,证实该分离物为番茄细菌性溃疡病菌,发病植株为接种体侵染所致。该研究结果表明,打顶接种方法比剪叶法、针刺法和浸根法更适合用于番茄溃疡病菌的致病性测定和评价不同番茄品种苗期的抗病性,具有应用价值。  相似文献   

Antiproliferative and Antimicrobial Potentials of a Lectin from Aplysia kurodai (Sea Hare) Eggs     

Rubaiya Rafique Swarna  A. K. M. Asaduzzaman  Syed Rashel Kabir  Nawshin Arfin  Sarkar M. A. Kawsar  Sultana Rajia  Yuki Fujii  Yukiko Ogawa  Keisuke Hirashima  Nanae Kobayashi  Masao Yamada  Yasuhiro Ozeki  Imtiaj Hasan 《Marine drugs》2021,19(7)

In recent years, there has been considerable interest in lectins from marine invertebrates. In this study, the biological activities of a lectin protein isolated from the eggs of Sea hare (Aplysia kurodai) were evaluated. The 40 kDa Aplysia kurodai egg lectin (or AKL-40) binds to D-galacturonic acid and D-galactose sugars similar to previously purified isotypes with various molecular weights (32/30 and 16 kDa). The N-terminal sequence of AKL-40 was similar to other sea hare egg lectins. The lectin was shown to be moderately toxic to brine shrimp nauplii, with an LC₅₀ value of 63.63 µg/mL. It agglutinated Ehrlich ascites carcinoma cells and reduced their growth, up to 58.3% in vivo when injected into Swiss albino mice at a rate of 2 mg/kg/day. The morphology of these cells apparently changed due to AKL-40, while the expression of apoptosis-related genes (p53, Bax, and Bcl-XL) suggested a possible apoptotic pathway of cell death. AKL-40 also inhibited the growth of human erythroleukemia cells, probably via activating the MAPK/ERK pathway, but did not affect human B-lymphoma cells (Raji) or rat basophilic leukemia cells (RBL-1). In vitro, lectin suppressed the growth of Ehrlich ascites carcinoma and U937 cells by 37.9% and 31.8%, respectively. Along with strong antifungal activity against Talaromyces verruculosus, AKL showed antibacterial activity against Staphylococcus aureus, Shigella sonnei, and Bacillus cereus whereas the growth of Escherichia coli was not affected by the lectin. This study explores the antiproliferative and antimicrobial potentials of AKL as well as its involvement in embryo defense of sea hare.  相似文献   

Effect of flunixin meglumine treatment during and after embryo transfer on the pregnancy rate in cattle     

Tahir Karasahin  Hasan Alkan  Fatma Satilmis  Sukru Dursun  Huseyin Erdem 《Reproduction in domestic animals》2021,56(12):1555-1561

This study aimed to determine the effect of flunixin meglumine treatment during and after the transfer of in vivo produced embryos to Angus (cows) and Holstein (cows and heifers) breeds of cattle on pregnancy rate. Holstein cows were used as donors in the study. A double dose of prostaglandin F2α was administered to the recipient animals for synchronization. Uterine flushing was performed in donors on day 7 after artificial insemination. A total of 295 transferable embryos were obtained. These embryos were transferred to Angus cows (n = 85), Holstein heifers (n = 80) and Holstein cows (n = 130). After the transfer, these animals were divided into three subgroups. The first subgroup (TI) was administered flunixin meglumine during embryo transfer, and the second subgroup (TII) was administered flunixin meglumine both during embryo transfer and on days 8 and 9 after the transfer. The third subgroup (TIII) was not administered anything and it was considered the control group. Pregnancy examination of the recipients was performed on days 30–35 after the transfer using real-time ultrasonography. The pregnancy rates after embryo transfer were found to be 43.52% in Angus cows, 42.5% in Holstein heifers, and 24.61% in Holstein cows (p < .05). When the animals were not classified according to breed, the pregnancy rates in subgroups TI, TII and TIII were found to be 29.29%, 45.10% and 29.79%, respectively (p < .05). In addition, the pregnancy rates were higher in TII and TIII subgroups of Angus cows and Holstein heifers compared to that of Holstein cows (p < .05). As a result, the pregnancy rates obtained after embryo transfer in Angus cows and Holstein heifers were found to be higher than that in Holstein cows. In addition, it was concluded that the administration of flunixin meglumine during and during/after embryo transfer has a positive effect on pregnancy rates in Angus cows and Holstein heifers.  相似文献