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Renan C. Lima Pablo H. Teixeira Ari F. F. Souza Trazilbo J. Paula Júnior Hudson Teixeira Miller S. Lehner José E. S. Carneiro Tiago S. Marçal Rogerio F. Vieira 《Plant pathology》2020,69(6):1172-1184
Development of common bean cultivars with partial white mould resistance through breeding techniques has been a challenge in Brazil. As yet, lines/cultivars from breeding programmes have not been investigated for resistance; therefore, this study screened 107 lines/cultivars for their reactions to white mould in 14 preliminary trials conducted under irrigation. Thirteen resistant lines/cultivars (three of Andean origin) and six Mesoamerican cultivars (three intermediately resistant and three susceptible) were selected for further investigation. These lines/cultivars and the resistant control A195 were evaluated in six advanced trials and two straw tests to assess the effectiveness of the screening procedure. In 11 preliminary trials, screenings were performed under moderate/high or higher disease pressure. These pressures occurred in two advanced trials in which, when yields were averaged across moderate/high and high pressures, 10 Mesoamerican lines/cultivars selected for resistance yielded 14%, 23%, and 38% more than intermediately resistant cultivars, A195, and susceptible cultivars, with median disease ratings (1–9 scale) of 4.5, 5.7, 5.7, and 6.7, respectively. In the straw test, three Andean lines/cultivars (A195 included) and two susceptible cultivars in the field were among those with the highest levels of physiological resistance. Thus, field rating under high disease pressure and greenhouse rating did not correlate significantly, suggesting that field trials are critical to evaluating resistance and to identifying high-yielding beans. Therefore, lines/cultivars from breeding programmes assessed in field trials may provide a low cost and fast way to identify high-yielding bean cultivars with partial resistance to white mould in the subtropical southern hemisphere. 相似文献
64.
Histological alterations in gills of shrimp Litopenaeus vannamei in low‐salinity waters under different stocking densities: Potential relationship with nitrogen compounds
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Marcela G. Fregoso‐López María S. Morales‐Covarrubias Miguel A Franco‐Nava Javier Ramírez‐Rochín Juan F Fierro‐Sañudo Jesús T Ponce‐Palafox Federico Páez‐Osuna 《Aquaculture Research》2017,48(12):5854-5863
Two experimental modules with different stocking densities (M1 = 70 and M2 = 120 shrimp /m2) were examined weekly over a culture cycle in tanks with low‐salinity water (1.9 g/L) and zero water exchange. Results showed survival rates of 87.7 and 11.9% in M1 and M2, respectively. Water temperature, pH, dissolved oxygen, electrical conductivity and chlorophyll a were not significantly (p > .05) different between modules. In contrast, the concentrations of nitrogen compounds were significantly (p < .05) different between modules, except nitrite‐N (M2 were 2.31 ± 1.38 mg/L N‐TAN, 0.18 ± 0.49 mg/L N‐NO2? and 6.83 ± 6.52 mg/L N‐NO3?; in M1: 0.97 ± 0.73 mg/L N‐TAN, 0.05 ± 0.21 mg/L N‐NO2? and 0.63 ± 0.70 mg/L N‐NO3?). When waters of both modules reached higher levels of ammonia and nitrite, histological alterations were observed in gills. The histological alterations index (HAI) was higher in M2 (5‐112) than in M1 (2‐22). 相似文献
65.
Laura Gmez-Montes Zaul García-Esquivel Louis R. D'Abramo Armando Shimada Carlos Vsquez-Pelez María Teresa Viana 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):769-780
Juvenile green abalone Haliotis rufescens were grown under laboratory conditions at 21±1 °C and fed formulated diets consisting of different protein:energy ratios (mg protein/kcal), 62, 74, 85, 100, 108, for 60 days. The level of crude protein ranged from approximately 26% to 44% while the energy content remained constant at about 4.1 kcal g−1. Growth ranged from 3.63 to 12.33 mg day−1. The growth of abalone fed the 100 and 108 diets was significantly greater than that of each of the other diets. Protein efficiency ratio increased as the dietary protein content increased except for the T108 diet (44% crude protein). Abalone apparently consume food to satisfy an energy requirement. Caloric expenditure due to metabolism was estimated for abalone fed diets with protein ratios of 62, 85, 100. Energy loss due to respiration did not vary appreciably among abalone fed the different diets. The proportional distribution of dietary energy into fecal, digestible, growth, and metabolic energy was estimated for abalone fed these diets. Apparent dry matter digestibility was among the lowest for abalone fed the 100 P:E diet, but growth of abalone fed this diet was significantly higher than that of each of the other treatments except the 108 diet. Unexplained energy loss to achieve balance ranged from 7% to 28.5%, some of which is probably due to differential mucus and ammonia production. Results suggest a potential for the reduction of both dietary protein and lipid without causing any adverse effects on the growth response. 相似文献
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Sigríur Gumundsdttir Niccol Vendramin Argelia Cuenca Heia Sigurardttir Arni Kristmundsson Tine Moesgaard Iburg Niels Jrgen Olesen 《Journal of fish diseases》2019,42(1):47-62
A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated from wild lumpfish (Cyclopterus lumpus), brought to a land‐based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identified as VHSV by ELISA and real‐time RT‐PCR. Phylogenetic analysis, based on the glycoprotein (G) gene sequences, may indicate a novel subgroup of VHSV genotype IV. In controlled laboratory exposure studies with this new isolate, there was 3% survival in the I.P. injection challenged group while there was 90% survival in the immersion group. VHSV was not re‐isolated from fish challenged by immersion. In a cohabitation trial, lumpfish infected I.P. (shedders) were placed in tanks with naïve lumpfish as well as naïve Atlantic salmon (Salmo salar L.). 10% of the lumpfish shedders and 43%–50% of the cohabiting lumpfish survived after 4 weeks. 80%–92% of the Atlantic salmon survived, but no viral RNA was detected by real‐time RT‐PCR nor VHSV was isolated from Atlantic salmon. This is the first isolation of a notifiable virus in Iceland and the first report of VHSV of genotype IV in European waters. 相似文献
68.
Izquierdo MS Robaina L Juárez-Carrillo E Oliva V Hernández-Cruz CM Afonso JM 《Fish physiology and biochemistry》2008,34(2):117-127
The Δ6 and Δ5 desaturases and elongases show only very limited activity in marine fish, and little is known of the possibility
of enhancing Δ6 desaturase gene expression in these fish. The use of plant oils in marine fish diets is limited by their lack
of n−3 highly unsaturated fatty acids (HUFA) despite an abundant content of the 18C fatty acid precursor linoleic and α-linolenic
acids. The objective of the present study was to determine the ability of larval gilthead seabream to utilize vegetable oils
and assess the nutritional regulation of Δ6 desaturase gene expression. Seventeen-day-old gilthead seabream larvae were fed
during a 17-day period with one of four different microdiets formulated with either sardine fish oil (FO), soybean, rapeseed
or linseed oils, respectively, or a fifth diet containing defatted squid meal and linseed oil. Good larval survival and growth,
both in terms of total length and body weight, were obtained by feeding the larvae either rapeseed, soybean or linseed oils.
The presence of vegetable oils in the diet increased the levels of 20:2n−9 and 20:2n−6, 18:2n−9, 18:3n−6, 20:3n−6 and 20:4n−6, in larvae fed rapeseed and soybean oils in comparison to those fed FO. In addition, a sixfold increase in the relative
expression of Δ6 desaturase-like gene was found in larvae fed rapeseed and soybean oils, denoting the nutritional regulation
of desaturase activity through its gene expression in this fish species. However, feeding linseed oil did not increase the
expression of the Δ6 desaturase gene to such a high extent. 相似文献
69.
Evaluation of shrimp polyculture system in Thailand based on stable carbon and nitrogen isotope ratios 总被引:1,自引:0,他引:1
HISASHI YOKOYAMA JUNYA HIGANO a KUMIKO ADACHI YUKA ISHIHI YOSHIHIRO YAMADA b 《Fisheries Science》2002,68(4):745-750
ABSTRACT: To quantify the contribution by cocultured animals to waste assimilation in an intensive shrimp farm in Thailand, the food web structures of the macrobenthos in a reservoir pond, a shrimp culture pond and water treatment ponds were examined using the stable C and N isotope ratio technique. Seawater for aquaculture was drawn from a creek, and stored in a reservoir pond, used for farming the banana prawn Fenneropenaeus merguiensis in culture ponds, and then recycled through treatment ponds where the green mussel Perna viridis was cultured to remove organic wastes discharged from the farming. The clam worm Nereididae sp. and the mud creeper Cerithideopsilla cingulata in the culture pond had δ 13 C values of −21.0‰ and −18.4‰, respectively, suggesting that shrimp feed (mean δ 13 C = −20.7‰) was the main food source for these species. The δ 13 C analysis also suggested that sediments (−23.7‰) in the reservoir pond and particulate organic matter (POM) (−24.0‰) and/or sediments (−25.0‰) in the treatment pond supplied carbon for most macrobenthic animals. However, green mussels in the treatment pond had a mean δ 13 C value of −20.5‰, suggesting that shrimp feed was the main food source for this species. 相似文献
70.