Chemical profile of white wines produced from 'Greco bianco' grape variety in different Italian areas by nuclear magnetic resonance (NMR) and conventional physicochemical analyses     

Caruso M  Galgano F  Castiglione Morelli MA  Viggiani L  Lencioni L  Giussani B  Favati F 《Journal of agricultural and food chemistry》2012,60(1):7-15

In this study the characterization of white wines produced from the monovarietal 'Greco bianco' grape variety is presented for the first time. A total of 40 commercial wines, from two different southern Italian regions, Calabria and Campania, from the same grape variety and two different vintages, were investigated. The analyses were performed by means of chromatographic methods, conventional analyses, and nuclear magnetic resonance (NMR) spectroscopy. No differentiation was observed according to the year of production but a significant discrimination was achieved using principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). In particular, PLS-DA allowed the selection of compounds (total acidity; citric, malic, succinic, and lactic acids; total polyphenol index; glucose and proline/arginine ratio) useful for differentiating the studied wines on the basis of geographical origin.  相似文献   

Specific primers for Xanthomonas vesicatoria, a tomato bacterial spot causal agent     

Edivânio R. Araújo  Marisa A. S. V. Ferreira  Alice Maria Quezado-Duval 《European journal of plant pathology / European Foundation for Plant Pathology》2013,135(1):5-9

Xanthomonas vesicatoria is a member of the species complex associated with tomato bacterial spot. New and specific primers for X. vesicatoria were developed and validated. The primers were highly specific and detection was positive using purified bacterial DNA, bacterial suspensions and foliar lesions. These primers represent an additional tool for detection and identification of one of the species involved in this important disease complex.  相似文献   

Improved evaluation of the genetic variability of Brazilian strains of Erwinia psidii with newly developed microsatellite markers     

Pollyane da Silva Hermenegildo  Lúcio Mauro Silva Guimarães  Jorge Luis Badel  Abi S. A. Marques  Acelino Couto Alfenas  Marisa A. S. Velloso Ferreira 《Plant pathology》2021,70(4):922-931

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Modelling influenza A H5N1 vaccination strategy scenarios in the household poultry sector in Egypt   总被引：1，自引：1，他引：0  

Ihab El Masry  Jolianne Rijks  Marisa Peyre  Nick Taylor  Juan Lubroth  Yilma Jobre 《Tropical animal health and production》2014,46(1):57-63

Highly pathogenic avian influenza (AI) due to H5N1 virus was first reported in Egypt in February 2006; since then, the government has allowed avian influenza vaccination in poultry. The present study evaluated the impact of AI vaccination in terms of cumulative annual flock immunity (CAFI): the percentage of bird × weeks protected by immunity. This evaluation took account of the combined effects of vaccination coverage, vaccine efficacy (VE), and different characteristics of household poultry production on the effectiveness of the adopted vaccination strategy (VS), and provided alternative options for improvement. The evaluation used a population and vaccination model that calculates the CAFI. Participatory approaches were employed in 21 villages to develop the vaccination and flock parameters required for the model. The adopted VS were compared in the model with three alternative VS scenarios in terms of the CAFI. Vaccination coverage varied among villages but was generally low (between 1 and 48 %; median 14 %). Under the adopted VS, the CAFI predicted for the villages ranged from 2 to 31 %. It was concluded that despite the enormous effort put into rural household poultry AI vaccination by the Egyptian government, village CAFI is unlikely to be maintained at the levels required to significantly reduce the virus load and restrict transmission. In HPAI-endemic countries that consider AI vaccination as one of the disease control options, the high cost of mass AI vaccination campaigns and their achievable benefits must be compared with other available control measures, which may include targeted vaccination. Achievable vaccination coverage, VE and the different characteristics of commercial and household (village) poultry production are key parameters determining the feasibility and cost-effectiveness of different AI vaccination strategies.  相似文献   

Phenotypic and genotypic differentiation of porcine Salmonella enterica subsp. enterica serovar Derby isolates     

Michael GB  Cardoso M  Rabsch W  Schwarz S 《Veterinary microbiology》2006,118(3-4):312-318

Sixty-two Salmonella enterica subsp. enterica serovar Derby isolates from slaughter pigs and meat products isolated in Southern Brazil were analyzed for their genomic relationships and for the presence of antimicrobial resistance genes. Twenty-four S. Derby isolates were indistinguishable by their subtracted restriction fingerprinting (SRF) pattern, XbaI- and BlnI-macrorestriction patterns, phage type, plasmid profile, and resistance pattern. In contrast to the BlnI-macrorestriction patterns, the XbaI-macrorestriction patterns were in good agreement with the results of SRF analysis and phage typing. Among the four phage types detected, PT10 and PT21 were the most common. The combination of all typing methods revealed a great diversity among the S. Derby isolates. All strains carried plasmids and the 60 resistant isolates showed at least tetracycline resistance. The resistance genes found were sul1 and/or sul2 (sulfonamide resistance), aadA2 (streptomycin/spectinomycin resistance), tet(A) (tetracycline resistance), tet(B) (tetracycline/minocycline resistance), bla(TEM) (ampicillin resistance), and dfrA14 (trimethoprim resistance). A correlation of the geno- and phenotypic characteristics with the origin of the isolates revealed a substantial temporal variation in the occurrence of specific S. Derby isolates in different independent pig production lines in Southern Brazil. The large number of resistant isolates underlined the potential risk that S. Derby isolates can pose to human health when they enter the food chain.  相似文献   

Quantitative assessment of velocities of the annulus of the left atrioventricular valve and left ventricular free wall in healthy cats by use of two-dimensional color tissue Doppler imaging     

Chetboul V  Sampedrano CC  Tissier R  Gouni V  Saponaro V  Nicolle AP  Pouchelon JL 《American journal of veterinary research》2006,67(2):250-258

OBJECTIVE: To analyze velocities of the annulus of the left atrioventricular valve and left ventricular free wall (LVFW) in a large population of healthy cats by use of 2-dimensional color tissue Doppler imaging (TDI). ANIMALS: 100 healthy cats (0.3 to 12.0 years old; weighing 1.0 to 8.0 kg) of 6 breeds. PROCEDURE: Radial myocardial velocities were recorded in an endocardial and epicardial segment, and longitudinal velocities were recorded in 2 LVFW segments (basal and apical) and in the annulus of the left atrioventricular valve. RESULTS: LVFW velocities were significantly higher in the endocardial than epicardial layers and significantly higher in the basal than apical segments. For systole, early diastole, and late diastole, mean +/- SD radial myocardial velocity gradient (MVG), which was defined as the difference between endocardial and epicardial velocities, was 2.2 +/- 0.7, 3.3 +/- 1.3, and 1.8 +/- 0.7 cm/s, respectively, and longitudinal MVG, which was defined as the difference between basal and apical velocities, was 2.7 +/- 0.8, 3.1 +/- 1.4, and 2.1 +/- 0.9 cm/s, respectively. A breed effect was documented for several TDI variables; therefore, reference intervals for the TDI variables were determined for the 2 predominant breeds represented (Maine Coon and domestic shorthair cats). CONCLUSIONS AND CLINICAL RELEVANCE: LVFW velocities in healthy cats decrease from the endocardium to the epicardium and from the base to apex, thus defining radial and longitudinal MVG. These indices could complement conventional analysis of left ventricular function and contribute to the early accurate detection of cardiomyopathy in cats.  相似文献   

Genotypic differences in polyembryo formation and somatic embryogenesis increment in wheat (Triticum aestivum L.), following 2,4-D treatment     

Rosario Linacero  Marisa G. López-Bilbao  Concepción Romero  David A. Laurie  Ana M. Vázquez 《Euphytica》1996,89(3):345-348

Summary In the course of experiments performed to obtain haploid wheat plants in which 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to developing spikes, it was found that three cultivars showed a different ability to produce polyembryos (Thatcher 20.19%, Chris 7.06%, Dollar 0%). This behaviour was related to their capacity to form somatic embryos. Diploid immature embryos cultured in vitro after 2,4-D treatment, gave a higher frequency of embryogenic callus in Thatcher and Chris than in Dollar. As the common factor in both experiments was the 2,4-D treatment we propose that the three cultivars showed a differential sensitivity to 2,4-D.  相似文献   

Remediation Actions by a Risk Assessment Approach: A Case Study of Mercury Contamination     

Sabrina Saponaro  Elena Sezenna  Luca Bonomo 《Water, air, and soil pollution》2005,168(1-4):187-212

The risk assessment procedure for identifying the remediation actions which may be adopted at a mercury contaminated site, when the plants are upgraded in the future, is proposed. The potentially active exposure/migration pathways in the future arrangement of the area will be due to Hg contaminated subsoil as a primary source (vapor inhalation and groundwater leaching) and to groundwater as a possible secondary source (transport to the point of compliance). The data of mercury concentration in the soil were acquired through environmental monitoring campaigns, and were processed to establish the three-dimensional distribution of contamination in subsoil, to locate sources and to define their geometrical and chemical characteristics. Speciation tests of mercury in the soil indicated that the most abundant species present were poorly leachable under the site-specific environmental conditions, confirming the coefficient distribution value obtained by the leaching tests. Analytical and numerical fate and transport modeling tools were used to locate digging zones in the contaminated subsoil, so as to reduce the possible groundwater contaminant loading and to avoid the down-gradient exceeding the concentration limit according to regulations. Remediation actions additional to civil works were required, which consists of soil digging within one contamination source, for about 22,200 m³ of soil. In order to evaluate the Hazard Index (HI) for human receptors due to Hg vapor inhalation, the air concentration of volatile mercury at the exposure point was estimated, based on direct measurements carried out at the site. Simulation gave HI values below 1 for all tested scenarios, suggesting that public health is protected without any additional actions to the already scheduled plant upgrading and digging for groundwater protection.  相似文献   

Fluoride impact on native tree species of the atlantic forest near Cubatão,Brazil     

Andreas Klumpp  Gabriele Klumpp  Marisa Domingos  Márcia Dias Da Silva 《Water, air, and soil pollution》1996,87(1-4):57-71

Air pollutant emissions from the industrial complex of Cubatão, Brazil, have led to a severe deterioration of the Atlantic Forest ecosystem. In a field study, leaves of the tree speciesTibouchina pulchra, Miconia pyrifolia, andCecropia glazioui were collected at four sites with different pollution characteristics. Leaf fluoride contents of the three species were found to be highly elevated in a valley near to fertilizer factories. In an area further from the emission sources, which in the past had been affected by fluoride pollution, fluoride concentrations inTibouchina andMiconia continued to be elevated. Preliminary exposure experiments using Tibouchina seedlings as accumulative indicators are reported.  相似文献   

Genetics of nodulation and nitrogen fixation in Brazilian soybean cultivars     

Marisa F. Nicolás  Carlos A. Arrabal Arias  Mariangela Hungria 《Biology and Fertility of Soils》2002,36(2):109-117

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