Commercial genetic testing for type 2 polysaccharide storage myopathy and myofibrillar myopathy does not correspond to a histopathological diagnosis     

Stephanie J. Valberg  Carrie J. Finno  Marisa L. Henry  Melissa Schott  Deborah Velez-Irizarry  Sichong Peng  Erica C. McKenzie  Jessica L. Petersen 《Equine veterinary journal》2021,53(4):690-700

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Autosomal dominant mutations affecting X inactivation choice in the mouse     

Percec I  Plenge RM  Nadeau JH  Bartolomei MS  Willard HF 《Science (New York, N.Y.)》2002,296(5570):1136-1139

X chromosome inactivation is the silencing mechanism eutherian mammals use to equalize the expression of X-linked genes between males and females early in embryonic development. In the mouse, genetic control of inactivation requires elements within the X inactivation center (Xic) on the X chromosome that influence the choice of which X chromosome is to be inactivated in individual cells. It has long been posited that unidentified autosomal factors are essential to the process. We have used chemical mutagenesis in the mouse to identify specific factors involved in X inactivation and report two genetically distinct autosomal mutations with dominant effects on X chromosome choice early in embryogenesis.  相似文献   

Brown spot of pear,an emerging disease in Argentina: identification and pathogenicity characterization of Argentinean Stemphylium vesicarium isolates     

Temperini  Carolina Virginia  Tudela  Marisa Andrea Aluminé  Gimenez  Gustavo Nestor  Di Masi  Susana Noemí  Pardo  Alejandro Guillermo  Pose  Graciela Noemí 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(3):529-544

European Journal of Plant Pathology - Recently, a new plant disease was detected in the rural area of the High Valley of Río Negro, in Northern Patagonia, Argentina, called “Brown Spot...  相似文献   

Changes in antioxidants and fruit quality in hot water-treated ‘Hom Thong’ banana fruit during storage     

Nittaya Ummarat  Tracie K. Matsumoto  Marisa M. Wall  Kanogwan Seraypheap 《Scientia Horticulturae》2011

The effects of hot water treatment on antioxidants and fruit quality were investigated in banana fruit of cv. Gros Michel (Musa acuminata, AAA Group, locally called cv. Hom Thong) by immersing fruits in hot water (50 °C) for 10 min, before storage at 25 °C for 10 days or 14 °C for the first 8 days followed by storage at 25 °C for the second 8 days until ripening. Quality parameters including peel color and pulp firmness indicated that hot water treatment helped to delay banana fruit ripening at both storage conditions. Hot water treatment decreased the levels of hydrogen peroxide (H₂O₂) and malonydialdehyde (MDA) during storage at 25 °C. Glutathione (GSH and GSSG) contents and the ratio of GSH/GSSG during fruit approaching ripening were significantly induced in hot water-treated fruits while ascorbic acid (AA) contents were slightly increased. In addition, the combined treatment increased free phenolics and flavonoids during storage. Results suggest that hot water treatment has led to an induction of antioxidants in banana fruits as indicated by an increase of antioxidants and a decrease of H₂O₂ during ripening, and all of which result in a delayed ripening of banana fruit.  相似文献   

Dynamics of Dissolved Forms of Carbon and Inorganic Nitrogen in Small Watersheds of the Coastal Atlantic Forest in Southeast Brazil     

Tatiana M. B. Andrade  Pl��nio B. Camargo  Daniela M. L. Silva  Marisa C. Piccolo  Simone A. Vieira  Luciana F. Alves  Carlos A. Joly  Luiz A. Martinelli 《Water, air, and soil pollution》2011,214(1-4):393-408

Based on the fact that streamwater quality reflects landscape conditions, the objectives of this study were: to investigate nitrogen (N), carbon (C), and major ion concentrations in six streams crossing minimally disturbed Atlantic Forest areas, with similar geomorphological characteristics; to determine N and C fluxes in one of these pristine streams (Indaiá); and assess the impact of human activity on the biogeochemistry of two other streams in the same region, crossing urbanized areas. The distribution pattern of carbon and inorganic nitrogen dissolved forms, as well as the major ion and biogenic gas concentrations in the streamwater, was similar in pristine streams, indicating that the C and N dynamics were determined by influence of some factors, such as climate, atmospheric deposition, geology, soil type, and land covering, which were analogous in the forested watersheds. The urban streams were significantly different from the pristine streams, showing low dissolved oxygen concentrations, high respiration rates, and high concentrations of carbon dioxide, dissolved inorganic nitrogen, dissolved inorganic carbon, and major ion. These differences were attributed to anthropogenic impact on water quality, especially domestic sewage discharge. Additionally, in the Indaiá stream, it was possible to observe the importance of rainfall over temporal dynamics of dissolved carbon forms, and also, the obtained specific flux of dissolved inorganic nitrogen was relatively elevated (approximately 11 kg ha^?1 year^?1). These results reveal the influence of human activity over the biogeochemistry of coastal streams and also indicate the importance N export of Atlantic Forest to the ocean.  相似文献   

Role of the cultivar in choosing Clementine fruits with a high level of health-promoting compounds     

Milella L  Caruso M  Galgano F  Favati F  Padula MC  Martelli G 《Journal of agricultural and food chemistry》2011,59(10):5293-5298

Thirteen cultivars and two hybrids of Clementine fruits (Citrus clementina Hort. Ex. Tan) cultivated in Italy were characterized according to pH, titratable acidity, total soluble solids, total polyphenols, carotenoids, vitamin C, hesperidin, rutin, narirutin and naringin and radical scavenging activity. The presence of rutin in Clementine fruit juice is reported for the first time here. The results indicated that all chemical parameters statistically differentiated each cultivar (P < 0.001). In particular, principal component analysis showed a clear discrimination of five cultivars from all the other varieties based on vitamin C and total polyphenols for the Caffin cultivar, which showed also the highest antioxidant activity; narirutin for the Etna hybrid cultivar; hesperidin, rutin and total soluble solids for the SRA 89 cultivar; and naringin, hesperidin and rutin for the Esbal cultivar. Moreover, the Mandalate hybrid cultivar showed the lowest antioxidant activity as well as vitamin C and total polyphenols content, while titratable acidity and naringin level were the highest. The antioxidant activity assessed in all the fruits was closely correlated with vitamin C and total polyphenols content, rather than with the flavonoid compounds.  相似文献   

Gill morphometry of the facultative air-breathing loricariid fish,Hypostomus plecostomus (Walbaum) with,special emphasis on aquatic respiration     

Sonia Aparecida Perna  Marisa Narciso Fernandes 《Fish physiology and biochemistry》1996,15(3):213-220

Gill respiratory surface area and oxygen consumption during aquatic respiration were measured in the facultative air-breathing loricariid fish,Hypostomus plecostomus. The fish did not surface to breathe atmospheric air in normoxic water; air-breathing was evoked by environmental hypoxia (water oxygen tension=35±2, mmHg) and did not show size-related threshold differences for air breathing.During gradual hypoxia, without access to atmospheric, air,H. plecostomus was found to be an oxyregulator and showed a reduced range of water oxygen tension in which the oxygen consumption remained constant in smaller fish. The critical oxygen tensions were 55 and 33 mmHg at 25°C for fish of 14–30 g and 31–80g body weight, respectively.The gill respiratory surface area (total lamellae area) is reduced, however, the lamellar frequency per mm of gill filament is high which facilitates the gas exchange. Moreover, the increase of gill respiratory surface area (b=0.666) is higher than the increase in routine VO₂ (b=0.338) showing a positive relationship between the gill respiratory surface area /VO₂ ratio and body mass (b=0.328); this indicates that the fish have greater gill respiratory surface area per unit of routine VO₂ as they grow.  相似文献   

New Invertebrate Vectors for PST,Spirolides and Okadaic Acid in the North Atlantic     

Marisa Silva  Aldo Barreiro  Paula Rodriguez  Paz Otero  Joana Azevedo  Amparo Alfonso  Luis M. Botana  Vitor Vasconcelos 《Marine drugs》2013,11(6):1936-1960

The prevalence of poisoning events due to harmful algal blooms (HABs) has declined during the last two decades through monitoring programs and legislation, implemented mainly for bivalves. However, new toxin vectors and emergent toxins pose a challenge to public health. Several locations on the Portuguese coast were surveyed between 2009 and 2010 for three distinct biotoxin groups [saxitoxin (PST), spirolide (SPX) and okadaic acid (OA)], in 14 benthic species of mollusks and echinoderms. Our main goals were to detect new vectors and unravel the seasonal and geographical patterns of these toxins. PSTs were analyzed by the Lawrence method, SPXs by LC-MS/MS, and OA by LC-MS/MS and UPLC-MS/MS. We report 16 new vectors for these toxins in the North Atlantic. There were differences in toxin contents among species, but no significant geographical or seasonal patterns were found. Our results suggest that legislation should be adjusted to extend the monitoring of marine toxins to a wider range of species besides edible bivalves.  相似文献   

Water stress and warming impact nutrient use efficiency of Mombasa grass (Megathyrsus maximus) in tropical conditions     

Dilier Olivera Viciedo  Renato de Mello Prado  Carlos A. Martinez  Eduardo Habermann  Roberto Botelho Ferraz Branco  Marisa de Cssia Piccolo  Alexander Calero Hurtado  Kolima Pea Calzada  Luis F. Lata Tenesaca 《Journal of Agronomy and Crop Science》2021,207(1):128-138

Temperature and other abiotic factors, such water and nutrient availability, play an important role for plants in response to the changing environments. At this regard, both warming and drought might affect the nutrient use efficiency (NUE) and growth of Megathyrsus maximus a C4 forage grass of high interest for cattle feeding. However, the nutrient requirements of this species under climate change are unknown. Therefore, we aimed to evaluate the individual and combined effects of two levels of temperature: ambient and elevated temperature (2°C above ambient temperature), and two levels of soil water availability: irrigated plants and non‐irrigated plants on accumulation of leaf nutrients, NUE and biomass production of M. maximus. Temperature control was performed by a temperature free‐air‐controlled enhancement (T‐FACE) system under field conditions. In general, we observed that warming under well‐irrigated conditions increased the leaf accumulation of most nutrients, improving the NUE of N, P, K, Ca, Mg, Cu, Mn and Zn. Plant growth was also enhanced under warming effects, with higher leaf dry mass accumulation and root development. Meanwhile, drought decreased NUE of K, Ca, B and leaf dry biomass, while root growth was stimulated. The combined effects of warming and drought on nutrient accumulation, NUE and plant growth tended to be greater than the individual effects expected from a single factor; thus, warming mitigated the negative impacts of individual drought. In summary, our findings suggest that warming and drought, both as individual and combined factors, will change the nutrient requirements of M. maximus in tropical ecosystems.  相似文献   

Use of recombinant human granulocyte colony-stimulating factor prior to autologous bone marrow transplantation in dogs with lymphoma     

Lane AE  Chan MJ  Wyatt KM 《American journal of veterinary research》2012,73(6):894-899

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