Pedologic analysis of the Danilo Bitinj Site, Central Dalmatia, Croatia     

Cynthia M. Fadem  Jennifer R. Smith  Andrew Moore  Marko Men&#x;u&#x;i&#x; 《CATENA》2009,78(3):181

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Oak ellagitannins suppress the phosphorylation of the epidermal growth factor receptor in human colon carcinoma cells     

Fridrich D  Glabasnia A  Fritz J  Esselen M  Pahlke G  Hofmann T  Marko D 《Journal of agricultural and food chemistry》2008,56(9):3010-3015

The ellagitannins castalagin and vescalagin, and the C-glycosides grandinin and roburin E as well as ellagic acid were found to potently inhibit the growth of human colon carcinoma cells (HT29) in vitro. In a cell-free system these compounds were identified as potent inhibitors of the protein tyrosine kinase activity of the epidermal growth factor receptor (EGFR) with IC 50 values in the low nanomolar range. To address the question of whether the interference with the activity of the isolated EGFR also plays a role within intact cells, effects on the phosphorylation status of the EGFR, as a measure for its activity, were determined in HT29 cells. As exemplified for castalagin and grandinin, both the nonglycosylated and the glycosylated ellagitannins effectively suppressed EGFR phosphorylation, but only at concentrations > or =10 microM, thus, in a concentration range where growth inhibition was observed. These results indicate that the suppression of EGFR-mediated signaling might contribute to the growth inhibitory effects of these compounds present in oak-matured wines and spirits such as whiskey. In contrast, despite substantial growth inhibitory properties, ellagic acid did not significantly affect EGFR phosphorylation in HT29 cells up to 100 microM.  相似文献   

Physicochemical properties and release characteristics of calcium alginate microspheres loaded with Trichoderma viride spores     

Slaven Jurić  Edyta Đermić  Snježana Topolovec-Pintarić  Marta Bedek  Marko Vinceković 《农业科学学报》2019,18(11):2534-2548

Novel agroformulations for simultaneous delivery of chemical and biologically active agents to the plants were prepared by encapsulation of Trichoderma viride spores in calcium alginate microspheres. The impact of calcium ions concentration on the viability and sporulation of T. viride spores as well as on the microsphere important physicochemical properties were investigated. Intermolecular interactions in microspheres are complex including mainly hydrogen bonds and electrostatic interactions. T. viride germination inside matrix and germ tubes penetration out of microspheres revealed calcium alginate microspheres provide a supportive environment for T. viride growth. Differences in physicochemical properties and bioactive agents release behaviour from microspheres were ascribed to the changes in microsphere structure. Fitting to Korsmeyer-Peppas empirical model revealed the underlying T. viride release mechanism as anomalous transport kinetics (a combination of two diffusion mechanisms and the Type II transport (polymer swelling and relaxation of the polymeric matrix)). The increasing amount of T. viride spores in the surrounding medium is closely related to the release from microspheres and germination. The rate controlling mechanism of calcium release is Fickian diffusion. A decrease in the release rate with increasing calcium ion concentrations is in accordance with the calcium ions effect on the strength of the alginate network structure. T. viride germination inside microsphere diminished the amount of released calcium ions and slowed release kinetics in comparison with microspheres prepared without T. viride. The results indicated investigated agroformulations have a great potential to be used for plant protection and nutrition.  相似文献   

Polycystic kidney disease: a review and occurrence in Slovenia with comparison between ultrasound and genetic testing     

Domanjko-Petric A  Cernec D  Cotman M 《Journal of Feline Medicine and Surgery》2008,10(2):115-119

Polycystic kidney disease (PKD) is an inherited autosomal kidney disease which is most commonly identified in Persian and Persian related cats. Positive cats have multiple cysts of various sizes that occur in the renal cortex and medulla and occasionally in other abdominal organs. PKD often leads to renal failure which occurs from mid to late in life. Renal cysts can be diagnosed ultrasonographically after 7 weeks of age by an experienced ultrasonographer and a high resolution machine. However, ultrasonography is now being replaced by genetic screening. A total of 340 cats of variable breeds aged from 5 months to 18 years were ultrasonographically examined in the past 7 years at the University Veterinary Small Animal Clinic. Of these, 13.8% were PKD positive with very high prevalence in Persian cats (36%). There was no sex predilection identified. The C>A transversion at position 3284 on exon 29 of PKD1 gene, resulting in a stop mutation has been identified in the heterozygous state in eight affected cats examined (Persian breed). All heterozygous cats were also ultrasonographically positive.  相似文献   

Effects of UV light irradiation on colour stability of thermally modified, copper ethanolamine treated and non-modified wood: EPR and DRIFT spectroscopic studies     

Manabendra Deka  Miha Humar  Gregor Rep  Borut Kričej  Marjeta Šentjurc  Marko Petrič 《Wood Science and Technology》2008,42(1):5-20

A series of experiments were carried out to investigate the colour stability of chemically treated and thermally modified wood compared to non-modified wood during long term artificial UV light irradiation. One set of wood samples was vacuum-pressure impregnated with alkaline (pH 9.8) copper (II) ethanolamine aqueous solution, while another set of samples from the same wood block was thermally modified at 210°C and −0.90 bar for 2 h. The treated and modified wood samples along with the non-modified ones were exposed to artificial UV light with the wave length in the region of UVA (315–400 nm) and UVB (280–315 nm) intermittently for 500 h. Colour measurements were carried out throughout the irradiation period at an interval of 100 h according to CIEL*a*b* system, where the results are presented in terms of ΔE, ΔL*, Δa* and Δb* values. Better photo-stability in terms of colour changes was recorded for both treated and modified woods compared to the non-modified one. By means of EPR and DRIFT spectroscopic study it was shown that some degree of colour stability of treated and modified woods, achieved during artificial UV light irradiation, resulted from lignin modifications and monomers of phenolic compounds.  相似文献   

Harvesting European knowledge on soil functions and land management using multi‐criteria decision analysis     

Francesca Bampa  Lilian O'Sullivan  Kirsten Madena  Taru Sandn  Heide Spiegel  Christian Bugge Henriksen  Bhim Bahadur Ghaley  Arwyn Jones  Jan Staes  Sylvain Sturel  Aneta Trajanov  Rachel E. Creamer  Marko Debeljak 《Soil Use and Management》2019,35(1):6-20

Soil and its ecosystem functions play a societal role in securing sustainable food production while safeguarding natural resources. A functional land management framework has been proposed to optimize the agro‐environmental outputs from the land and specifically the supply and demand of soil functions such as (a) primary productivity, (b) carbon sequestration, (c) water purification and regulation, (d) biodiversity and (e) nutrient cycling, for which soil knowledge is essential. From the outset, the LANDMARK multi‐actor research project integrates harvested knowledge from local, national and European stakeholders to develop such guidelines, creating a sense of ownership, trust and reciprocity of the outcomes. About 470 stakeholders from five European countries participated in 32 structured workshops covering multiple land uses in six climatic zones. The harmonized results include stakeholders’ priorities and concerns, perceptions on soil quality and functions, implementation of tools, management techniques, indicators and monitoring, activities and policies, knowledge gaps and ideas. Multi‐criteria decision analysis was used for data analysis. Two qualitative models were developed using Decision EXpert methodology to evaluate “knowledge” and “needs”. Soil quality perceptions differed across workshops, depending on the stakeholder level and regionally established terminologies. Stakeholders had good inherent knowledge about soil functioning, but several gaps were identified. In terms of critical requirements, stakeholders defined high technical, activity and policy needs in (a) financial incentives, (b) credible information on improving more sustainable management practices, (c) locally relevant advice, (d) farmers’ discussion groups, (e) training programmes, (f) funding for applied research and monitoring, and (g) strengthening soil science in education.  相似文献   

Genetic Diversity and Redundancy Among Potato Accessions in the Montenegrin Collection as Revealed by Microsatellite Markers     

Marko Maras  Aleš Sedlar  Alex Reid  Vladan Božović  Zoran Jovović  Vladimir Meglič  Peter Dolničar 《American Journal of Potato Research》2017,94(4):306-313

Potato was introduced in Montenegro in the middle of the eighteenth century. Since then it has become the most important crop in plant production. During the period between 2008 and 2010 a total of 52 potato accessions was collected across Montenegro and stored in a national gene bank. In the study reported here 23 accessions from the collection were examined using microsatellite (also known as simple sequence repeats or SSRs) molecular markers with the aim to explore genetic diversity and redundancy within the germplasm. The accessions were selected on the basis of preliminary characterization of all 52 accessions for 11 lightsprout traits. Molecular characterization of 23 accessions by 12 SSR markers was carried out at SASA (Science and Advice for Scottish Agriculture) that manages a database of more than 3000 genetic profiles of potato from Europe and abroad. Comparison of SSR genetic profiles of Montenegrin collection against the existing SASA database allowed us to test the authenticity of the Montenegrin accessions. Out of the 23 accessions examined, 13 showed distinct genetic profiles of which seven showed perfect matching with known cultivars, two profiles showed strong similarity to another two cultivars, and four profiles were found unique with regards to the SASA database. Application of microsatellite markers in this study provided valuable information on the extent of genetic diversity residing within Montenegrin potato germplasm; it gave clear indications of the scale of redundancy within the collection; and helped clarify the identity of the accessions. Four accessions within the collection might incorporate unique variation and will be subjected to further agronomical examinations to assess their potential for breeding purposes.  相似文献   

Regional intravenous gentamicin administration for treatment of postoperative tarso-metatarsal infection in a dog--a case report     

Vnuk D  Stejskal M  Musulin A  Maticić D 《Berliner und Münchener tier?rztliche Wochenschrift》2012,125(3-4):172-175

The regional intravenous antibiotic administration has been used to achieve high local concentrations of antibiotics into the extremities. The goal of this case report was to describe a known, but not often used technique of local administration of antibiotics for treatment of acute soft tissue, joint or bone infection in a dog, which suffered from chronic renal failure. In a seven years old Great Dane tarso-metatarsal arthrodesis was performed and three days after surgery infection was obvious. Gentamicin-sensitive E. co i was found by antibiogram. A tourniquet was tightened on the affected limb and gentamicin was administered in a dosage of 0,5 mg/kg every twelve hours via an intravenous catheter placed in the saphenous vein. The therapy lasted for ten days and wound healing was progressive. Regional intravenous gentamicin administration was very effective in treating distal extremity infection.  相似文献   

Landscape properties and precipitation as determinants for high summer nitrogen load from boreal catchments     

Lauri Arvola  Eeva Einola  Marko Järvinen 《Landscape Ecology》2015,30(3):429-442

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Effect of RNF4-SacII gene polymorphism on reproductive traits of Landrace × Large White crossbred sows     

Sven Menčik  Vlado Vuković  Zhihua Jiang  Mario Ostović  Velimir Sušić  Ivona Žura Žaja  Marko Samardžija  Anamaria Ekert Kabalin 《Reproduction in domestic animals》2020,55(10):1286-1293

The aim of the present study was to investigate whether and to what extent the RNF4-SacII gene polymorphism influences reproduction performances in hyperprolific sow lines. The study involved 101 Landrace x Large White crossbred sows, with 461 records collected on the following reproductive traits: Total Number of piglets Born per litter (TNB), Number of piglets Born Alive per litter (NBA), Number of StillBorn piglets per litter (NSB), piglet Pre-Weaning Mortality (PWM) and Number of piglets at Weaning per litter (NW). The least square method with the GLM procedure in SAS with eight effects was used to pursue the data analysis. Study results revealed that TT homozygotes and TC heterozygotes had a significantly higher (p < .05) NW than CC homozygotes for the life-span performance in all parities and first parity analysed. In the fourth parity analysed, TNB and NBA in TC genotype were significantly higher (p < .05) as compared with TT genotype. Based on the life-span performance, significant effect (p < .05) was recorded for order of parity on TNB, NBA and NW, for farrowing season on TNB and NSB, and for lactation length on PWM. In the second parity, significant effect (p < .05) was recorded for sire of boar on NSB and for gestation length on TNB. Only in the fourth parity, significant effect (p < .05) of RNF4 gene was observed on NBA. There was significant additive effect (p < .05) of the RNF4 gene polymorphism identified on NW in all parities analysed, and significant additive and dominance effects (p < .05) on NSB in the third parity analysed. In conclusion, additional research on related production pig genotypes is necessary to elucidate the effect of RNF4 gene mutation on reproductive traits.  相似文献