Metabolomic analysis of Ranunculus spp. as potential agents involved in the etiology of equine grass sickness     

Michl J  Modarai M  Edwards S  Heinrich M 《Journal of agricultural and food chemistry》2011,59(18):10388-10393

Identification of toxic or harmful agents continues to be a key goal in agricultural chemistry. This paper reports a metabolomic analysis of Ranunculus repens and related species, which were recently postulated to be cocausative agents in the etiology of equine grass sickness (EGS). Specifically, samples collected at EGS sites were compared with those from non-EGS sites. Furthermore, interspecific and seasonal variations and the species' response to edaphic and climatic factors were investigated. (1)H NMR spectroscopy in combination with multivariate data analysis was applied to the crude methanol extracts of the Ranunculus samples, as well as their chloroform fractions. Samples from EGS sites were significantly different from control samples. The metabolite composition varied greatly between different Ranunculus species. No significant changes could be observed between samples collected in different seasons. This work provides strong evidence that Ranunculus is involved in the etiology of EGS and has implications for agricultural management of pastures.  相似文献   

Screening drought-tolerant genotypes in bread wheat (Triticum aestivum L.) using different multivariate methods     

Roohollah Abdolshahi  Abdorahim Safarian  Maryam Nazari  Shahram Pourseyedi  Ghasem Mohamadi-Nejad 《Archives of Agronomy and Soil Science》2013,59(5):685-704

Wheat (Triticum aestivum L.) is one of the most widely cultivated crops in rainfed areas of Iran, where drought is the main limiting factor on yield. The object of this study was the identification of drought-tolerant genotypes in bread wheat. Forty bread wheat genotypes were tested in separate experiments under drought stress and normal conditions in two years (2009–2010 and 2010–2011). Nine drought-tolerance/susceptibility indices including stress susceptibility index (SSI), mean productivity (MP), tolerance (TOL), stress tolerance index (STI), geometric mean productivity (GMP), yield index (YI), yield stability index (YSI), linear regression coefficient (β) and drought response index (DRI) were determined. Simultanously applied factor analysis used two factors instead of nine indices in this study. Mahdavi was recognized as the most drought-tolerant genotype in both years based on factor analysis. In this study an equation was developed for estimating the Stress Tolerance Score (STS). The results of the equation were identical to those of factor analysis in both years. The equation was much easier to use than factor analysis and is suggested as a screening tool for the identification of drought-tolerant genotypes. In this study, Mahdavi was the most drought-tolerant genotype also corresponding to this equation.  相似文献   

Improvement of Antioxidant System and Decrease of Lignin by Nickel Treatment in Tea Plant     

Faezeh Ghanati  Maryam Rahmati Ishka 《Journal of plant nutrition》2013,36(9):1649-1661

The effect of nickel (Ni) on the activities of antioxidant enzymes was evaluated in suspension-cultured cells of tea (Camellia sinensis L. cv. ‘Yabukita’) and two-year-old rooted cuttings of tea (Camellia sinensis L. assamica × sinensis). Suspension-cultured cells were grown in B5 medium and treated with 40 μM of Ni for 24 h. Tea plants were grown in a modified Hoagland solution and treated for one week with 40 μM of Ni. The activities of superoxide dismutase, catalase, ascorbate peroxidase, and ionically wall-bound peroxidase of both Ni-treated tea cells and Ni-treated tea plants increased compared with those of control groups. The activities of soluble peroxidase and covalently wall-bound peroxidase, as well as the lignin content of both tea cells and tea plants, decreased under Ni treatment. Thus it seems that Ni may have beneficial effects on tea plants via elevation of antioxidant activity and decrease of lignification of the walls.  相似文献   

Effects of Coenzyme Q10 on the ratio of TH1/TH2 in Experimental Autoimmune Encephalomyelitis Model of Multiple Sclerosis in C57BL/6     

Maryam Soleimani  Seyed Behnamedin Jameie  Mahmood Barati  Mehdi Mehdizadeh  Mahdieh Kerdari 《Iranian Biomedical Journal》2014,18(4):203-211

Background: Multiple sclerosis (MS) is known as a progressive central nervous system inflammatory disease. Certain factors, such as interleukins, inflammatory cells, and oxidative stress are supposed to involve in MS etiology. Because of the important role of oxidative stress, antioxidant therapy for MS has received more attention. Although coenzyme Q10 (CoQ10) acts as an antioxidant, there is a lack of enough research on its effects on MS. Therefore, the present research was designed. Methods: C57BL/6 female adult mice (n = 30) were used in this study. The animals were randomly divided into trial and control groups. To induce MS, routine procedure for experimental autoimmune encephalomyelitis (EAE) was used, and scoring was performed based on clinical signs. By detecting score one, CoQ10 administration was started (10 mg/kg/three weeks). By using ELISA and real-time PCR, the brain levels of TNF-, IL-10, IL-4, and IL-12 were studied. Statistical tests were used to analyze the data and the P value less than 0.05 was considered to be significant. Results: Clinical symptoms in EAE animals were significantly decreased (P<0.05) as compared to control ones. In addition, the level of the TNF- was significantly decreased following CoQ10 administration versus IL-10. The ratio of TH1/TH2 interleukins in treated animals was significantly less than that in non-treated animals (P<0.01). Conclusion: Our findings showed that CoQ10 is capable of suppressing the inflammatory pathway of MS. Key Words: Experimental autoimmune encephalomyelitis (EAE), Multiple Sclerosis (MS), Coenzyme Q10 (CoQ10)  相似文献   

Pre-treatment effect of different doses of soy isoflavones on spatial learning and memory in an ovariectomized animal model of Alzheimer's disease     

Sarkaki A  Amani R  Badavi M  Moghaddam AZ  Aligholi H  Safahani M  Haghighizadeh MH 《Pakistan journal of biological sciences: PJBS》2008,11(8):1114-1119

The aim of this study was to evaluate the effects of different doses of dietary soy meals (with or without isoflavone) on dementia in ovariectomized (OVX) animal model of Alzheimer's disease. Female Wistar's rats with the exception of intact group were ovariectomized at the first line of study. Animals were divided into 2 main groups: control (c) and pre-treatment groups. Animals in pre-treatment groups received one of five types of diet during four weeks prior Nucleus Basalis Magnocellularis (NBM) electrical lesion normal diet (0), 10 g soy with isoflavone (10), 20 g soy with isoflavone (20), 10 g soy without isoflavone (-10) and 20 g soy without isoflavone (-20) in 30 g daily diet. The spatial learning and memory were tested using Morris water maze after electrical lesion. Rats were trained in water maze to find a hidden escape Platform. Rats received 6 blocks that each block consisted of 3 trials. Following acquisition trials, one probe trial was conducted in which the platform was removed. Soy meal diet (with or without isoflavone) in ovariectomized rats with Alzheimer's disease caused improvement of performance across 18 trials of Acquisition. Our results suggest that soy meal is a potential alternative to estrogen in the prevention and treatment of Alzheimer's disease.  相似文献   

Epidemiology of taeniosis,cysticercosis and trichinellosis in Iran: A systematic review     

Mohammad Moazeni  Faham Khamesipour  Douglas N. Anyona  Gabriel O. Dida 《Zoonoses and public health》2019,66(1):140-154

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Osteopontin gene polymorphism association with milk traits and its expression analysis in milk of riverine buffalo     

Sidra Manzoor  Asif Nadeem  Javed Maryam  Abu Saeed Hashmi  Muhammad Imran  Masroor Ellahi Babar 《Tropical animal health and production》2018,50(2):275-281

Osteopontin gene is regarded as a plausible candidate in mammary gland differentiation and development, expressed by variety of cells, tissues, and biological fluids including milk. The current study was performed in two phases. In the first phase, Osteopontin gene polymorphisms were identified and associated with milk composition such as ash, milk fat, SNF, lactose, and protein. In the second phase, milk samples from five healthy mastitis-free Nili Ravi buffaloes were analyzed for expression of Osteopontin gene at transition (day 15), mid (day 90), and end (day 250) stage of their second lactation. Briefly, blood samples were collected from Nili Ravi buffalo to isolate the genomic DNA, specific primers were designed for PCR amplification. The amplified PCR products were sequenced bi-directionally. Six polymorphisms were identified in the coding region and four in the intronic region of the gene. The results showed that SNP g.38329758 T > C causing substitution of valine to alanine (V127A) was associated with high milk protein. For mRNA expression analysis, somatic cells were separated from milk samples for RNA isolation. Analysis of differential gene expression data has permitted us to illustrate the expression pattern of osteopontin gene in lactating buffalo. The Osteopontin gene was found to be transcribed among all three lactation stages, but expression was observed with the highest value (fold change) in peak lactation and remained elevated till the end of lactation. Identified gene marker may be helpful for the prediction of superior animal for selection. The presented study also gave an insight into the genetic screening and lactation biology of riverine buffalo, offering direction for future research in lactating buffalo.  相似文献   

Dietary sour lemon (Citrus limon) peel essential oil supplementation for reduction of deltamethrin‐induced stress in rainbow trout (Oncorhynchus mykiss)     

Maryam Amiri Resketi  Sakineh Yeganeh  Khosro Jani Khalili 《Journal of the World Aquaculture Society》2021,52(1):105-123

The purpose of this study was to investigate the dietary effects of using essential oil from sour lemon peel (Citrus limon) on growth performance, carcass composition, blood and serum parameters, and liver enzymes of juvenile rainbow trout (Oncorhynchus mykiss) exposed to deltamethrin (DMN) pesticide. Treatments with no lemon peel essential oil (LPEO) and without DMN (control), as well as four treatments with 10% of 96‐h lethal concentration of DMN were prepared with dietary supplementation of LPEO (zero, 200, 400, and 600 mg/kg). Trout juveniles (95.12 ± 0.53 g) were randomly allocated to the treatments (each with three replicates). The results showed that dietary LPEO inclusion improved fish growth parameters in the DMN‐containing treatments. The highest fat content was found in zero LPEO treatment with no DMN while it was lowest in DMN treatments containing 400 and 600 mg/kg of LPEO. Carcass protein content was the highest in DMN treatment with 400 mg/kg of LPEO. Blood and serum biochemical parameters showed statistical differences between no LPEO treatment with DMN exposure and those detected in the control fish (p < .05). Overall, the results indicate that dietary inclusion of LPEO at 400 mg/kg level can decrease some negative effects of DMN exposure.  相似文献   

Lemon,Citrus aurantifolia,peel and Bacillus licheniformis protected common carp,Cyprinus carpio,from Aeromonas hydrophila infection by improving the humoral and skin mucosal immunity,and antioxidative responses     

Faeze Sadeghi  Ehsan Ahmadifar  Mohsen Shahriari Moghadam  Maryam Ghiyasi  Mahmoud A. O. Dawood  Sevdan Yilmaz 《Journal of the World Aquaculture Society》2021,52(1):124-137

The role of dietary lemon peel (LM) and/or Bacillus licheniformis (BL) on the growth, immunity, and resistance against Aeromonas hydrophila in common carp, Cyprinus carpio was investigated in this study. LM and BL were included in diets at 0% (T0), 10⁸ CFU/g BL (T1), 1.5% LM and 10⁸ CFU/g BL (T2), and 3% LM and 10⁸ CFU/g BL (T3). Fish fed with T1, T2, or T3 had higher weight gain, specific growth rate, white blood cells count, and blood total protein with lower feed conversion ratio than T0 group (p < .05). The albumin increased significantly (p < .05) in fish fed both BL and LM (T3). The serum superoxide dismutase (SOD), catalase, lysozyme, and bactericidal activities were significantly increased in fish fed both BL and LM (T2 and T3), while serum glutathione peroxidase increased in fish fed BL (T2) (p < .05). Fish fed T1, T2, and T3 diets displayed higher SOD and lower malondialdehyde than fish fed T0 (p < .05). After the A. hydrophila challenge, the mortality rate was significantly lower in T1, T2, and T3 groups than the T0 group (p < .05). The obtained results revealed that LM and BL could be used to increase resistance against A. hydrophila infection in carp. However, further field studies should be performed to confirm the obtained results.  相似文献   

Pigmentation and growth performance in the blue gourami,Trichogaster trichopterus,fed marigold,Calendula officinalis,powder, a natural carotenoid source     

Maryam Jorjani  Mostafa Sharif Rohani  Amin Mirhashemi Rostami  Harry Ako  Aileen Tan Shau Hwai 《Journal of the World Aquaculture Society》2019,50(4):789-799

The present study aimed to determine the effects of different levels of marigold, Calendula officinalis, powder, 0 (control), 0.5% (M0.5), 1.5% (M1.5), and 2.5% (M2.5), on pigmentation and growth performance of the blue gourami after a 70‐day trial. At the end of the experiment, no significant differences (p > 0.05) were found in fish growth performance and body compositions. Color parameters (L*, a*, and b*) were weekly evaluated by examining a point posterior to the fish operculum. The marigold‐fed fish were darker than the control fish at Weeks 9 and 10. Yellowness intensity of the M2.5 was significantly higher than the other treatments at Week 9. Skin and caudal fin total carotenoids, astaxanthin, canthaxanthin, and β‐carotene contents of the treatment M2.5 were significantly different (p < 0.05) compared to the other treatments. Muscle total carotenoids, astaxanthin, and canthaxanthin contents of the marigold‐treated fish were significantly (p < 0.05) higher than the control group. Muscle β‐carotene contents of the treatments M1.5 and M2.5 were significantly (p < 0.05) higher than the control group. This study shows that marigold powder may be considered an efficient natural carotenoid source for pigmentation in blue gourami.  相似文献