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211.
Hiroyuki Sato Makoto Miura Takeshi Fujieda Marcin Taciak Motoi Kikusato Kan Sato Masaaki Toyomizu 《Animal Science Journal》2021,92(1):e13605
Seventy-five individually fed barrows averaging 35 kg were used in a series of three experiments for 6-week growth assays (25 barrows each) to determine the effects of l -tryptophan (Trp) supplementation on growth performance and to establish the lower limits of dietary crude protein (CP) levels. Corn and soybean meal (SBM)-based diets containing 9% (Experiment 1), 10% (Experiment 2), and 11% CP (Experiment 3) fortified with deficient essential amino acids (AAs) except Trp were used as basal diets for each experiment. The experimental diets were supplemented with 0.00%, 0.02%, 0.04%, or 0.06% Trp. A 16% CP corn–SBM-based diet was set as a positive control in each experiment, and feed and water were provided ad libitum. Average daily gain and gain-to-feed ratio improved quadratically (P < 0.05) as supplemented Trp increased in the 9% and 10% CP group, although these positive effects were not observed in the 11% CP group. Because the maximum performance parameters in 9%, 10%, and 11% CP groups were not different from that of the 16% CP positive control group, the marginally reduced level of dietary CP without growth performance being affected appears to be around 9% at most. A potential reduction of nitrogen intake was clearly indicated. 相似文献
212.
Chutima Khomvilai Masaaki Kashiwagi Chanin Sangrungruang Motoi Yoshioka 《Fisheries Science》2006,72(1):28-32
ABSTRACT: To protect chum salmon eggs from water mold infection during incubation, the eggs were treated daily with sodium hypochlorite solution (NaOCl) at 10 mg/L residual chlorine concentration for 15 min during their developmental period from fertilization to eyed stages. The number of infected eggs and number of eyed eggs were observed on day 23 of incubation. The percentage of infected eggs to total eggs was significantly lower with NaOCl treatment (1.8 : 33.4%) than in the control (11.3 : 59.3%, P < 0.01). The percentage of eyed eggs to total eggs was significantly higher with NaOCl (85.9 : 98.6%) than in the control (66.1 : 97.5%, P < 0.01). The antifungal activity of NaOCl resulted in improving egg survival. Accordingly, NaOCl is a useful antifungal agent against water mold infection on chum salmon eggs. 相似文献
213.
Shunsuke Shimamura Risa Kainuma Ken Kimura Yasuhiko Okamura Saori Kobayashi Masaaki Katayama Reeko Sato Jun Yasuda 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(4):366-368
A 13-year-old castrated male miniature dachshund dog was presented with chronic vomiting and diarrhea. Contrast radiography and surgical exploration revealed an ileal cyst, which was excised. A diagnosis of epidermoid cyst was made from a combination of the clinical findings and histological examination of the surgical specimen. 相似文献
214.
215.
本研究将白鱚卵的胚胎发生过程严格区分为27个阶段,并就各不同发生阶段的卵对高温突变、落下冲击和紫外线照射的感受性进行了详细的比较研究,得出了卵裂早期和原肠晚期至胚孔封闭期的卵对高温突变的感受性高;卵裂晚期至胚体出现期,对落下冲击的感受性高;对紫外线照射的感受性,则以胚体出现期为最高。文中同时分析了这种不同环境因素作用于相同鱼卵的同一发生阶段,产生不同影响的原因;建议在鱼类资源保护和鱼苗生产的孵化管理上,应尽量避免感受性较高的相应环境因素变化对鱼卵的影响。 相似文献
216.
Hongyu Darhan Atsushi Zoda Motoi Kikusato Masaaki Toyomizu Kazuo Katoh Sang‐gun Roh Shinichiro Ogawa Yoshinobu Uemoto Masahiro Satoh Keiichi Suzuki 《Animal Science Journal》2019,90(7):818-826
The aims of the present study were to identify the differences between two mouse lines (high (H)‐ and low (L)‐oxygen consumption) in terms of mitochondrial respiratory activity when GMP (glutamate, malate, and pyruvate) and succinic acid are used as substrates and to examine the relationship between mitochondrial respiration activity and feed efficiency in both lines. The average daily feed intake, feed conversion ratio (FCR), and residual feed intake (RFI) were significantly higher in the H than the L line. The correlation between FCR and RFI was significant (r = 0.60, p < 0.05). RFI was effective as an indicator of feed efficiency. When succinic acid was used as a substrate, mitochondrial respiration states 2–4, ACR, and proton leak were significantly higher in the H than the L line. When GMP was used as a substrate, respiration states 3 and 4 in the H line were significantly higher than those in the L line, and there were significant positive correlations between FCR and RFI and mitochondrial respiration states 2–4. The results indicated that selection for high or low OC changed the basal metabolic rates estimated from liver mitochondrial respiration activity and feed efficiency. 相似文献
217.
Saishu YOSHIDA Hideaki YURINO Masaaki KOBAYASHI Naoto NISHIMURA Kentaro YANO Ken FUJIWARA Shin-ichi HASHIMOTO Takako KATO Yukio KATO 《The Journal of reproduction and development》2022,68(3):225
Pituitary endocrine cells are supplied by Sox2-expressing stem/progenitor cells in the anterior lobe of the adult pituitary gland. These SOX2-positive cells are maintained in two types of microenvironments (niches): the marginal cell layer (MCL)-niche and the parenchymal-niche. Recently, we isolated dense SOX2-positive cell clusters from the parenchymal-niche by taking advantage of their resistance to protease treatment as parenchymal stem/progenitor cell (PS)-clusters. In the present study, by analyzing these isolated PS-clusters, we attempted to identify novel structural characteristics of pituitary stem/progenitor cell niches. Quantitative real-time PCR showed that tight junction-related genes were distinctly expressed in the isolated PS-clusters. Immunocytostaining showed that the tight junction molecules, ZO-1 and occludin, were localized in the apical membrane facing the pseudo-follicle-like structure of the isolated PS-clusters regardless of the expression of S100β, which distinguishes the sub-population of SOX2-positive cells. Furthermore, immunohistochemistry of the pituitary glands of adult rats clearly demonstrated that ZO-1 and occludin were densely present in the parenchymal-niche encircling the pseudo-follicle, while they were observed in the apical membrane in the MCL-niche facing the residual lumen. Collectively, these tight junction-related proteins might be involved in the architecture and maintenance of the plasticity of pituitary stem/progenitor cell niches. 相似文献
218.
Wheat starch granules were separated from waxy wheat flour by the acetic acid (pH 3.5) fractionation technique. Waxy wheat starch samples were stained red‐brown using KI/I2 solution. Subsequently, the concentration of the KI/I2 solution was increased to >2.5% KI and 1.0% I2. Then the appearance of the waxy wheat starch granules quickly became ghostlike. The internal structure of the ghost could be observed. This structure consisted of two parts with different colors: a red‐brown central part and a slightly pinkish surrounding part. Sonication of the ghost at 20 kHz for 20 sec separated the central part from the surrounding part. Each of the separated parts were subjected to gel‐filtration chromatography, and their chromatography profiles indicated only amylopectin and no amylose. The central and surrounding parts had molecular weights of 54.3 × 105 and 23.8 × 106 from Berry plots, respectively. 相似文献