菜豆新品种将军油豆的选育   总被引：1，自引：1，他引：1  

冯国军  曲敏  刘大军  闫田柱  王玉莉  叶永亮 《中国蔬菜》2004,1(5):22-23

将军油豆是以农家品种大马掌为母本 ,家雀蛋为父本人工杂交后系统选育而成。中熟 ,蔓生 ,从播种到采收 6 5d(天 ) ,生长势强 ,嫩荚绿色 ,扁条形 ,荚尖部有红条纹 ,平均荚长 2 0cm ,荚宽 2 .4cm ,单荚质量 2 4g ,纤维少 ,肉质面 ,属典型的东北优质油豆角类型。抗炭疽病、锈病 ,适应性广 ,春秋皆可种植 ,露地、保护地兼用  相似文献   

体验经济与内蒙古草原旅游开发   总被引：10，自引：0，他引：10  

刘敏  石学勇 《干旱区资源与环境》2004,18(5):128-131

本文通过捕捉近年来消费情况的变化 ,认为应对体验经济引起重视。在介绍体验经济的提出 ,体验的概念和体验的组成部分的基础上 ,提出体验经济对旅游具有创造差异化竞争优势、准确把握市场、使旅游者更舒适愉悦等意义 ,并在体验经济理论下结合内蒙古草原旅游的实际情况 ,提出内蒙古草原的体验设计和营销策略  相似文献   

二棘血蜱叮咬中华硬蜱纯化抗原免疫接种宿主后的交叉免疫反应     

刘志刚  叶炳辉  崔晓民  徐敏 《中国生物防治》2004,20(2):95-98

选用中华硬蜱105kD柱层析纯化抗原对新西兰兔进行免疫接种,再用二棘血蜱进行叮咬,并与单纯二棘血蜱叮咬组和佐剂对照组进行对比,以探讨不同种属硬蜱之间的交叉免疫抗性。结果显示:单纯二棘血蜱叮咬组吸血量为181 3±44 3mg,而二棘血蜱叮咬由中华硬蜱105kD纯化抗原免疫接种组和佐剂对照组新西兰兔后其吸血量分别为102 1±25 3mg和168 5±40 9mg,纯化抗原免疫接种组较单纯二棘血蜱叮咬组和佐剂对照组吸血量分别下降43 7%和39 4%(P<0 01)。同时,纯化抗原免疫接种组也较单纯二棘血蜱叮咬组和佐剂对照组产卵量有显著下降。该研究结果表明中华硬蜱和二棘血蜱之间存在着交叉免疫反应。  相似文献   

Differential regulating effect on estrogen receptor α and β expression in female rat hippocampus and cortex regions between different types of estrogen regents     

JIN Min  HUANG He-feng  JIN Fan 《园艺学报》2004,20(8):1422-1426

AIM: To study the regulatory effect two different estrogen reagents on expressions of estrogen receptor α and β in female rat hippocampus and cortex regions. METHODS: 12 cycles after ovariectomy, female rats were orally injected with premarin or progynova for 3 cycles before sacrificed. Semiquantitative RT-PCR was used to detect the ERs mRNA expression and SP immunohistochemistry was performed to measure the ERs protein distribution and expression. RESULTS: In premarin group, ER α mRNA levels in both hippocampus and cortex tissues decreased significantly compared with control. ER α protein level in hippocampus was lower than that in the control. However, ER α protein level in cortex had no statistical difference. ER β mRNA in the two regions and ER β protein in cortex had no statistical differences compared with control, while ER β protein level in hippocampus was higher than that in the control. In progynova group, both mRNA and protein levels of ER β increased significantly in the two regions compared with the control, and ER α mRNA level also increased in hippocampus, but ER α mRNA level in cortex and ER α protein levels in the above two regions showed no statistical differences. CONCLUSION: There were differential regulatory effects on ER α and ER β expression in female rat cognitive regions between the two different types of estrogen reagents, which may be one of the mechanisms of varied effects in different estrogen replacement therapy reagents.  相似文献   

Antiviral Effect of Saccharomyces cerevisiaeβ‐glucan to Swine Influenza Virus by Increased Production of Interferon‐γ and Nitric Oxide     

K. Jung  Y. Ha  S.‐K. Ha  D. U. Han  D.‐W. Kim  W. K. Moon  C. Chae 《Zoonoses and public health》2004,51(2):72-76

The aim of these experiments was to investigate the potential antiviral effect of Saccharomyces cerevisiaeβ‐glucan on the pneumonia induced by swine influenza virus (SIV). Forty colostrum‐deprived 5‐day‐old piglets were randomly divided into four groups of 10. The 20 pigs in groups 1 and 2 were administered Saccharomyces cerevisiaeβ‐glucan orally (50 mg/day/pig; En‐Bio Technology Co., Ltd) for 3 days before SIV infection and those in groups 3 and 4 were given culture medium/diluent alone. Groups 1 and 3 were inoculated intranasally with 3 ml of tissue culture fluid containing 2 × 10⁶ tissue culture infective doses 50% (TCID₅₀)/ml of SIV and those in groups 2 and 4 were exposed in the same manner to uninfected cell culture supernatant. The microscopic lung lesions induced by SIV infection (group 1 pigs) were significantly more severe than those induced by infection in animals pre‐administered β‐glucan (group 3) (P < 0.05). Significantly more SIV nucleic acid was detected in the lungs of pigs experimentally infected with SIV only (group 1) at 5, 7 and 10 days post‐inoculation (dpi) compared with lungs from pigs pre‐administered β‐glucan and infected with SIV (group 3) (P < 0.05). The concentrations of interferon‐γ (IFN‐γ) and nitric oxide (NO) in bronchoalveolar lavage fluid from pigs pre‐administered β‐glucan and infected with SIV (group 3) were significantly higher than for any other group at 7 and 10 dpi for IFN‐γ, and at 5, 7 and 10 dpi for NO (P < 0.05). Saccharomyces cerevisiaeβ‐glucan reduced the pulmonary lesion score and viral replication rate in SIV‐infected pigs. These findings support the potential application of β‐glucan as prophylactic/treatment agent in influenza virus infection.  相似文献   

Analysis of a Secreted Aspartic Peptidase Disruption Mutant of Glomerella cingulata     

Kim M. Plummer  Sarah J. Clark  Lana M. Ellis  Ashwini Loganathan  Taha H. Al-Samarrai  Erik H.A. Rikkerink  Patrick A. Sullivan  Matthew D. Templeton  Peter C. Farley 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(3):265-274

Peptidases have been implicated in the pathogenicity of fungi that cause disease in plants. Expression of the secreted aspartic peptidase gene (gcsap), of a Glomerella cingulata isolate pathogenic on apples, is induced during appressorium formation. To determine whether the secreted aspartic peptidase (GcSAP) is essential to pathogenicity, gcsap was disrupted using a vector containing a 637 bp fragment of genomic DNA that encodes the sequence spanning the two active site aspartic acid (Asp) residues. To ensure that the truncated gcsap gene products could not have residual peptidase activity the codons for the active site residues Asp¹¹² and Asp²⁹⁷ were both mutated to histidine residues. Both PCR and Southern analysis confirmed disruption of gcsap. Neither gcsap mRNA nor GcSAP activity was detected in the disruption mutant. Pathogenicity tests on fruit from three apple cultivars showed that GcSAP was not required for pathogenicity. The disruption mutant grew on medium containing protein as the sole source of nitrogen because G. cingulata secretes a previously undetected peptidase(s). A serine peptidase that had a pH optimum between pH 7.0 and 8.0 and a K _m of 0.25 mM for the synthetic substrate succinyl-Ala–Ala–Pro–Phe-p-nitroanilide was identified.  相似文献   

遗传标记及其在家禽育种中的应用   总被引：1，自引：1，他引：1  

M.G.Emara  H.Kim  吴艳 《中国家禽》2005,27(7):41-42

1 SNP SNP是染色体中遗传变异的最普通形式,如在人体已经检测基因组DNA中每1,000到2,000个单位有1个SNP,在某些区域估计每300个单位有一个SNP.基于SNP的高分辨率,遗传图谱在一些人的染色体上通常没有得到发展.正如其名称所指,SNP是发生在基因(启动子、外显子、或内含子)或基因之间(基因间隔区)的单个碱基改变或核苷酸变异.编码序列中的SNP以同义(不会导致氨基酸改变)或非同义(导致氨基酸改变)来分类.非同义SNP由于对蛋白质表达及最终对表现型的潜在影响而受到重视.相反,同义SNP可能对基因表达有微小的影响(除那些在启动子和其它基因组区域中,对DNA-蛋白质作重要的核苷酸或与RNA稳定性有关的核苷酸).同义和非同义SNP在基因图谱研究中均是很好的遗传标记.  相似文献   

中国水牛瘤胃纤毛虫分布与挥发性脂肪酸的研究     

桂荣  赵青余  那日苏  张民  杨炳壮  黄峰 《畜牧兽医学报》2005,36(12):1286-1291

对中国广西西林水牛和摩拉水牛瘤胃纤毛虫种类分布进行了鉴定检测,并测定了其瘤胃液挥发性脂肪酸浓度,同时进行了比较研究。全部检出17属63种25型瘤胃纤毛虫,比以前进行的8种反刍动物瘤胃纤毛虫分布研究中出现的总数和每个宿主个体中出现的平均纤毛虫种数多,种类构成最复杂,其纤毛虫构成特点类似于东南亚地区水牛瘤胃纤毛虫构成特点。其中广西西林水牛中检出了Entodinium biconcavumsp n,是至今从未鉴定记载过的认为是新种的特殊种类。广西西林水牛检出13属54种20型、摩拉水牛检出16属45种11型瘤胃纤毛虫。广西西林水牛瘤胃纤毛虫平均密度为2.13×105/mL,摩拉水牛瘤胃纤毛虫密度为3.43×105/mL。中国水牛瘤胃纤毛虫属别构成中Entodinium属出现率最高,但两个水牛品种间存在明显差别,其他属别构成也不尽一致。这与不同品种原来的地理环境分布与饲养管理条件有关。广西西林水牛瘤胃总VFA浓度为75.60 mmol/mL,摩拉水牛的为58.74 mmol/mL。瘤胃总VFA含量中均为乙酸含量最高,属于典型的粗饲料发酵类型。  相似文献   

Pharmacokinetic profiles of the two major active metabolites of metamizole (dipyrone) in cats following three different routes of administration          下载免费PDF全文

B. Lebkowska‐Wieruszewska  T. W. Kim  B. Chea  H. Owen  A. Poapolathep  M. Giorgi 《Journal of veterinary pharmacology and therapeutics》2018,41(2):334-339

This study was performed to determine pharmacokinetic profiles of the two active metabolites of the analgesic drug metamizole (dipyrone , MET), 4‐methylaminoantipyrine (MAA), and 4‐aminoantipyrine (AA), after intravenous (i.v., intramuscular (i.m.), and oral (p.o.) administration in cats. Six healthy mixed‐breed cats were administered MET (25 mg/kg) by i.v., i.m., or p.o. routes in a crossover design. Adverse clinical signs, namely salivation and vomiting, were detected in all groups (i.v. 67%, i.m. 34%, and p.o. 15%). The mean maximal plasma concentration of MAA for i.v., i.m., and p.o. administrations was 148.63 ± 106.64, 18.74 ± 4.97, and 20.59 ± 15.29 μg/ml, respectively, with about 7 hr of half‐life in all routes. Among the administration routes, the area under the plasma concentration curve (AUC) value was the lowest after i.m. administration and the AUC_EV/i.v. ratio was higher in p.o. than the i.m. administration without statistical significance. The plasma concentration of AA was detectable up to 24 hr, and the mean plasma concentrations were smaller than MAA. The present results suggest that MET is converted into the active metabolites in cats as in humans. Further pharmacodynamics and safety studies should be performed before any clinical use.  相似文献   

Effects of oral administration of different dosages of carvacrol essential oils on intestinal barrier function in broilers          下载免费PDF全文

ShuDong Liu  MinHo Song  Won Yun  JiHwan Lee  ChangHee Lee  WooGi Kwak  NamSoo Han  HyeunBum Kim  JinHo Cho 《Journal of animal physiology and animal nutrition》2018,102(5):1257-1265

Essential oils are widely used in the pharmaceutical, food and cosmetic industries, and many plant essential oils have shown that they have positive effects on broilers nutrition. This experiment was conducted to study the effects of orally administered different dosages of carvacrol essential oils on intestinal barrier function in broiler chickens. A total of eighty 28‐day‐old (1.28 ± 0.15 kg) ROSS 308 broilers were randomly allocated to four groups of 20 replicates each, with one chicken per replicate per cage, and all were fed with the same diet. Four experimental groups were orally administered 0, 200, 300 or 400 μl carvacrol essential oils at 18:00 hr every day during the 2‐week experimental period. As a result of which, the gene expression of the occludin, claudin‐1, claudin‐5, ZO‐1 and ZO‐2 in intestinal mucosa of small intestine (p < 0.05) and the goblet cell content in small intestine epithelium (p < 0.05) were significantly increased; test subjects with 300 or 400 μl carvacrol essential oils reduced the microbial counts of Salmonella spp. and Escherichia coli in the intestines (p < 0.05); Essential oils administration also significantly increased activity of the sucrase (p < 0.05) and lactase (p < 0.05) in intestinal mucosa. In conclusion, the carvacrol essential oils have positive effects on growth performance and intestinal barriers function of broilers; those effects may be related to the dosage, as administration of 300 or 400 μl was more effective than that of 200 μl.  相似文献