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121.
The combination of two analytical methodologies (water vapor sorption isotherm by using the DVS and chemical surface composition by using the XPS) has been used to enhance the understanding of the impact of re-grinding on the wheat flour hydration mechanism. A controlled atmosphere microbalance was used to construct water sorption isotherms at 25 °C of different samples of wheat flours obtained by successive re-grinding of native wheat flour.  相似文献   
122.
The present study assessed the effect of feeding palm oil (PO), sunflower oil (SO) and their combination on performance, fat deposition, fatty acid composition and lipogenic gene expression of broilers reared for 42 days. A total of 144 1‐day‐old broilers (Cobb500) were randomly allotted into four treatment diets with each having six replicates of six chicks in each replicate following a completely randomized design. Live weight gain and feed efficiency was significantly (P < 0.05) higher in birds fed with a combination of oil sources compared to controls. Birds fed with the combination of oil and SO alone had higher carcass yield and lower abdominal fat. Higher (P < 0.05) concentrations of unsaturated fatty acids (UFA) and lower concentrations of palmitic acid and saturated fatty acid (SFA) was found in birds fed SO alone and combinations of SO and PO. Furthermore, the outcomes showed that birds fed diet supplemented with SO and the combination of SO and PO down‐regulated gene expression of key hepatic lipogenic enzymes of fatty acids synthase (FAS), acetyl‐CoA carboxylase (ACC) and stearoyl‐CoA desaturase (SCD). These findings suggest that the diet containing the combination of 2% PO and 4% SO may reduce hepatic lipogenesis, as well as lower abdominal fat content of broilers.  相似文献   
123.
The aim of this research was to identify the changes in the cytoplasmic ultrastructure of immature and matured oocytes in buffalo (Bubalus bubalis ). Oocytes were matured in vitro in tissue culture medium?199 with and without sericin, and then analyzed by light and transmission electron microscopy. The experiment result showed that the nuclear maturation rate of buffalo oocytes was significantly higher in the presence of sericin (80.6%) than without sericin (68.1%) (P  < 0.05). The immature oocytes were characterized by cortical granule clusters in the ooplasm and the absence of perivitelline space (PVS ). In contrast, the oocytes matured either with or without sericin showed the formation of PVS , erected microvilli, the migration of cortical granules to the cytoplasmic periphery, and the clear appearance of the mitochondria and vesicle in the oolemma. Interestingly, matured oocytes with sericin have smaller cortical granules than do immature oocytes (P  < 0.05). In conclusion, supplementation of 0.05% sericin in the maturation medium can enhance the maturation rate of buffalo oocytes. Several cytoplasmic ultrastructures were relocated and modulated during the in vitro maturation process of buffalo oocytes: PVS development, cortical granules migration to periphery, and mitochondria and vesicles in the cortical region. The ultrastructure was similar between the groups with and without sericin.  相似文献   
124.
The purpose of this article is to present the diseases induced in humans and animals by the different species of Chlamydophila, after providing an overview on the history of these infectious agents and their taxonomy. The route of transmission and the available methods for prevention and control in the different animal species are reviewed.  相似文献   
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We isolated a novel single copy gene encoding a 57-kDa merozoite protein of Babesia gibsoni (BgP57). The nucleotide sequence of the cDNA was 2387 bp with an open reading frame (ORF) of 1644 bp encoding a 57-kDa predicted polypeptide having 547 amino acid residues. The recombinant BgP57 (rBgP57) without a predicted signal peptide was expressed in Escherichia coli as a soluble glutathione S-transferase (GST) fusion protein. Western blotting showed that the corresponding native protein was 57-kDa, consistent with molecular weight of predicted mature polypeptide. An indirect enzyme-linked immunosorbent assay (ELISA) using the rBgP57 detected specific antibodies in the sequential sera from a dog experimentally infected with B. gibsoni. Moreover, the antigen did not cross-react with antibodies to B. canis sub-species and closely related apicomplexan parasites indicating that the rBgP57 was a specific antigen for B. gibsoni antibodies. The diagnostic performance of ELISA based on rBgP57 using 107 sera from B. gibsoni-naturally infected dogs was the same as the previously identified rBgP32 but performed better than the previously studied rBgP50. Although, seminested PCR detected higher proportions (82%) of positive samples than the ELISAs, the Mcnemar's chi-square test showed that there was no significant difference in relative effectiveness of rBgP57-ELISA and seminested PCR (chi(2)=2.70; P=0.1003) in identifying positive samples. The rBgP57-ELISA when used in combination with rBgP32-ELISA and rBgP50-ELISA appeared to improve sensitivity of the rBgP57-ELISA for detection of B. gibsoni antibodies. Overall, the rBgP57-ELISA and seminested PCR when used in combination, could improve epidemiological surveys and clinical diagnosis of B. gibsoni infection.  相似文献   
127.

Many attempts/efforts have been made to cope with the fluctuated production performance in rice production in Malaysia. There is a crucial need in technological advancement to tackle the issue of environmental variability, decreasing productivity and the rising cost in rice cultivation. The use of variable rate technology has increased rice yield through efficient resource allocation. Notwithstanding, an evaluation of the feasibility of this technology is required in terms of economic and intangible benefits. Therefore, this study attempted to determine the potential benefits that could come out of the use of precision agriculture technologies in rice cultivation. A precision farming technology package was developed by the Malaysian Agricultural Research and Development Institute. It consists of two components, namely a variable rate seed application system and a variable rate fertilizer application system. The study estimated the monetary benefits gained from the use of these two technology components at Federal Land Consolidation and Rehabilitation Authority Seberang Perak. The study also compared the performance of the technology package against the conventional practice. The Cost–Benefit Analysis exercise has been implemented to show the benefits and implications comprised in the study. It was found that there is an additional net income per hectare in between Malaysian Ringgit (MYR) 1109 to MYR 1333 due to the reduction in the production cost if the precision farming technology packages were adopted. The advancement in two cultivation stages showed a positive impact monetarily for both small-scale and large-scale farmers in the study. However, many challenges are to be faced for the technologies implementation since the existing rice cultivation recommended practices throughout all stages even yet to be practiced and taken seriously.

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128.
129.
Water movement in a soil–plant system was evaluated based on capillary flow in a modified subsurface irrigation system that incorporates a plant-water measuring device. Water from a reservoir tank located underneath the plant pot was supplied to the root zone through a fibrous medium. Evapotranspiration was measured from the water uptake and evaluations were performed based on soil moisture distribution and mass balance. Potential evapotranspiration was used as a reference for the plant–water uptake. Data were obtained from a test plant provided with the modified subsurface irrigation system. The plant was grown in a phytotron under controlled air temperature and humidity, and a comparison was made for different levels of soil moisture condition. The experimental results confirmed the operational efficiency of the modified subsurface irrigation system for precision irrigation.  相似文献   
130.
Background: Stroke is the third leading cause of death. Hypothermia has been recognized as an effective method in reducing brain injury. In this study, we assessed the effects of granulocyte colony-stimulating factor (G-CSF) as a neuroprotective agent and mild hypothermia on mortality, behavioral function, infarct volume, and brain edema in Wistar rats. Methods: Forty male rats were used in five groups (eight rats in each group): control, hypothermy, G-CSF, combination hypothermy + CSF, and sham. Rats were anesthetized by injection of chloral hydrate (400 mg/kg) intraperitoneally. Transient cerebral ischemia was induced by 60-min intraluminal occlusion of left middle cerebral artery. Hypothermia, initiated at the time of reperfusion and G-CSF was started one hour after reperfusion at a dose of 15 mg/kg subcutaneously. The motor behavior was measured using Garcia’s index and animals were assigned for the assessments of infarction, brain swelling, and mortality rate. Results: The mortality was 38.46% (control group) and reduced in other groups. Neurological deficit score of control group (40.31 ± 1.56) was significantly lower than in treatment groups. The total cerebral infarct volume of treatment group was significantly lower than control group (43.96 ± 44.05 mm3). Treatment with hypothermy plus G-CSF (2.69 ± 0.24%) could significantly reduce brain swelling volume than other treatment groups. Conclusion: Our major finding is that mild hypothermic treatment plus G-CSF significantly reduced mortality rate and edema and improved neurological function. The results suggest that the combination of hypothermia and G-CSF is more effectively than other treatment groups being used alone. Key Words: Granulocyte colony-stimulating factor (G-CSF), Rats, Brain ischemia, Hypothermia  相似文献   
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