Molecular,biochemical and histopathological studies on the ameliorative effect of vitamin C on the renal and muscle tissues of Nile tilapia fish (Oreochromis niloticus) affected by the usage of engine oil     

Shaymaa H. Mohamed  Ebtihal M. M. EL‐Leithy  Rehab A. Ghandour  Mona K. Galal 《Aquaculture Research》2019,50(11):3357-3368

The present study was performed to evaluate the effect of vitamin C on the performance of Nile tilapia (Oreochromis niloticus) affected by the usage of engine oil. A total of 120 tilapia fish were randomly divided into four equal groups according to dietary treatments applied for 3 weeks: group I (control); group II was exposed to engine oil; group III was exposed to engine oil and supplemented with vitamin C: and group IV was fed only a diet supplemented with vitamin C. According to our results, the group exposed to engine oil (II) showed an induced elevation of kidney function parameters, an upregulation of CYP1A1 mRNA expression level, a reduction in antioxidant status (SOD, GSH) and severe abnormal histological and ultra‐structural changes in the head kidney, trunk kidney and muscle tissue. Supplementation of group III with vitamin C ameliorated the toxic effect induced by engine oil through improvement of kidney function parameters, elevation of the antioxidant status of tissue and improvement of the histological images of renal and muscle tissues. In conclusion, vitamin C has the ability to ameliorate engine oil oxidative stress toxicity in Nile tilapia.  相似文献   

Diverse conserved domains and a positively selected site in the sugarcane streak mosaic virus P1 protein are essential for RNA silencing suppression and protein stability     

Jian-Sheng Chen  Shan-Shan Liang  Sheng-Ren Sun  Mona B. Damaj  Hua-Ying Fu  San-Ji Gao 《Plant pathology》2020,69(7):1390-1400

RNA silencing is one of the conserved antiviral mechanisms in plants, and viruses encode RNA silencing suppressors (RSS) to overcome host RNA silencing and facilitate virus infection. Sugarcane streak mosaic virus (SCSMV; species Sugarcane streak mosaic virus, genus Poacevirus, family Potyviridae) is a major causal agent of sugarcane mosaic disease in many countries in Asia, including China. In this study, we used Agrobacterium co-infiltration to show that the SCSMV P1 protein, rather than the helper component-proteinase (HC-Pro), functions as a strong RSS to suppress local RNA silencing in Nicotiana benthamiana. Mutational analysis indicated that the 15 amino acids (aa; aa 1–15) of the SCSMV P1 N-terminus were not important for RNA silencing suppression, but rather another 15 aa domain (aa 108–122) containing a conserved motif (LFR/KNKQAYIST) was essential for efficient silencing suppression by P1. In addition to the 15 aa (aa 344–358) domain in the P1 N-terminus, another 15 aa domain (aa 65–79) of P1, containing the LXKA motif and one conserved aa (D78), were associated with P1 protein stability. Furthermore, substituting the histidine (H263) residue in P1 with threonine (H263T) or alanine (H263A) also affected P1 protein stability. Notably, the H263 residue is both a positively selected site and part of the serine protease catalytic triad (HDS). Taken together, our data demonstrate that SCSMV P1, and not HC-Pro, plays a functional role in suppressing RNA silencing, and also show that some conserved motifs and a positivelyselected site in the P1 protein are associated with RSS activity and protein stability.  相似文献   

Einfluss des Alters von Aphis gossypii Glover (Hom., Aphididae) als Beute auf die Prädationsleistung der polyphagen, räuberischen Wanze Dicyphus tamaninii Wagner (Het., Miridae)     

Cetin Sengonca  Azzam Saleh  Peter Blaeser 《Gesunde Pflanzen》2002,54(3-4):61-65

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On the healing of bone fractures in birds. Roentgenological clinical and histological study     

W Zedler  M Saleh 《Berliner und Münchener tier?rztliche Wochenschrift》1965,78(13):249-254

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Significance of the detachment of mesenterium for the blood supply in the dog     

M Saleh 《Archiv fuer experimentelle veterinaermedizin》1966,20(5):947-957

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Contribution to the Nasolacrimal Duct of Donkeys in Egypt     

A. H. Said    M. Shokry    M. A. Saleh  A. A. Hegazi 《Anatomia, histologia, embryologia》1977,6(4):347-350

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Epidural anaesthesia in donkeys.     

M Shoukry  M Saleh  K Fouad 《The Veterinary record》1975,97(23):450-452

Epidural anaesthesia was tested on 40 donkeys. The second intercoccygeal space was found suitable for satisfactory induction and a dose of 8 to 10 ml of 1 per cent procaine hydrochloride was recommended for inducing posterior epidural anaesthesia and 30 ml of 2 per cent for anterior epidural anaesthesia.  相似文献   

Malignant teratoid medulloepithelioma with brain and kidney involvement in a dog     

Aleksandersen M  Bjerkås E  Heiene R  Heegaard S 《Veterinary ophthalmology》2004,7(6):407-411

ANIMAL STUDIED: A tumor of the left eye with involvement of the brain and kidney was diagnosed in a 4-year-old Neapolitan mastiff. PROCEDURE: The dog presented with acute glaucoma of the left eye. Peripheral corneal vascularization and severe corneal edema obscured examination of deeper structures. Because of concurrent progressive neurologic signs the dog was euthanized and a postmortem examination was performed. The eyes, brain and samples from the lung, heart, liver and kidneys were fixed in 4% neutral buffered formalin and embedded in paraffin wax. Sections were examined by light microscopy including histochemical and immunohistochemical staining. RESULTS: The ocular tumor originated from the ciliary body and was composed of small islets and cords of poorly differentiated, oval to polyhedral cells surrounded by abundant ground substance. Areas with chondroid differentiation were observed. Rosettes were not found. Metastases with the same morphology were present in the brain and in one kidney. CONCLUSION: An intraocular malignant teratoid medulloepithelioma with metastases to the brain and kidney is presented.  相似文献   

Dietary Chitosan Nanoparticles: Potential Role in Modulation of Rainbow Trout (Oncorhynchus mykiss) Antibacterial Defense and Intestinal Immunity against Enteric Redmouth Disease     

Fatma Ahmed  Faiza M. Soliman  Mohamed A. Adly  Hamdy A. M. Soliman  Mansour El-Matbouli  Mona Saleh 《Marine drugs》2021,19(2)

Bio-nanotechnology employing bio-sourced nanomaterial is an emerging avenue serving the field of fish medicine. Marine-sourced chitosan nanoparticles (CSNPs) is a well-known antimicrobial and immunomodulatory reagent with low or no harm side effects on fish or their human consumers. In this study, in vitro skin mucus and serum antibacterial activity assays along with intestinal histology, histochemical, and gene expression analyses were performed to evaluate the impact of dietary CSNPs (5 g kg⁻¹ dry feed) on rainbow trout resistance against ‘enteric redmouth’ disease. Two treatment conditions were included; short-term prophylactic-regimen for 21 days before the bacterial challenge, and long-term therapeutic-regimen for 21 days before the challenge and extended for 28 days after the challenge. Our results revealed higher antibacterial defense ability and positive intestinal histochemical and molecular traits of rainbow trout after dietary CSNPs. The prophylactic-regimen improved trout health while the therapeutic regimen improved their disease resistance and lowered their morbidity. Therefore, it is anticipated that CSNPs is an effective antibacterial and immunomodulatory fish feed supplement against the infectious threats. However, the CSNPs seem to be more effective in the therapeutic application rather than being used for short-term prophylactic applications.  相似文献   

Plant polyphenols can directly affect ovarian cell functions and modify toluene effects     

Alexander Sirotkin  Zuzana Záhoranska  Adam Tarko  Zuzana Fabova  Saleh Alwasel  Abdel Halim Harrath 《Journal of animal physiology and animal nutrition》2021,105(1):80-89

The influence of toluene alone and in combination with plant polyphenols apigenin, daidzein or rutin on viability, proliferation (proliferating cell nuclear antigen accumulation), apoptosis (Bax accumulation) and release of progesterone (P), testosterone (T) and estradiol (E) in cultured porcine ovarian granulosa cells was evaluated. Toluene reduced ovarian cell viability, proliferation and E release; it promoted P release, demonstrating no effect on apoptosis or T output. Apigenin alone failed to affect cell viability, proliferation, apoptosis and P and T release, but stimulated E release, promoting the inhibitory action of toluene on proliferation, preventing and even reversing the stimulatory effect of toluene on apoptosis and P. Daidzein alone reduced cell viability and promoted T release, preventing and reversing the stimulatory effect of toluene on cell proliferation. Rutin administration reduced cell viability and E output, promoting the inhibitory action of toluene on cell viability and stimulatory effect on P release, and preventing the inhibitory action of toluene on E release. Toluene reduced apigenin- and rutin-induced E release, promoting action of daidzein on cell viability. These observations suggest the action of toluene and plant polyphenols on ovarian cell functions and the functional interrelationships between these molecules in the ovary.  相似文献