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111.
Journal of Plant Diseases and Protection - The rice root-knot nematode (Meloidogyne graminicola) is one of the key pests of rice (Oryza sativa L.) in irrigated and upland rice ecosystems inflicting...  相似文献   
112.
The use of fluoride enriched groundwater for irrigating the paddy crops is prevalent in many parts of the world. The fluoride in the irrigated water not only affects the growth and productivity of the crops, but is also accumulated in the plant tissues. We studied the accumulation of fluoride in roots, leaves and seeds two paddy varieties (i.e. Oryza sativa L. var. IR-36 and Oryza sativa L. var. Swarno) when treated with different concentrations of fluoridated water. The translocation pattern of fluoride was also observed. The water soluble fluoride ( $ {\text{F}}_{{{\text{H}}_{ 2} {\text{O}}}} $ ) in the soil and plant parts increased substantially with increasing fluoride concentration in treated water. The Swarno variety showed a slight but consistent higher uptake of fluoride than the IR-36 variety. Fluoride uptake by the germinated seedlings was many folds higher than the uptake by mature plants. At 30 mg l?1 fluoride exposure, the mean $ {\text{F}}_{{{\text{H}}_{ 2} {\text{O}}}} $ accumulation (mg kg?1 dry weight) in root, leaves, and seeds of Swarno was 54.1, 51.4 and 42.3, whereas the corresponding values in IR-36 were 50.9, 48.5 and 39.2. For the same exposure, the fluoride accumulation in root and shoot of Swarno and IR-36 seedlings were 3,480, 3,463 and 3,386, 3,360 mg kg?1, respectively. Normally, the fluoride accumulation follows the order of soil > root > shoot > grain. However, at early stage of fluoride contamination (5 mg l?1 NaF) roots tended to hyper accumulate fluoride from the soil.  相似文献   
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Mondal SK  Ray B  Thakur S  Ghosal PK 《Fitoterapia》2001,72(3):263-271
The water-soluble polysaccharides isolated from the vascular gel of Musa paradisiaca, were fractionated via anion exchange chromatography into four fractions. Fractionated polymers contained arabinose, xylose and galacturonic acid as major sugars, together with traces of galactose, rhamnose, mannose and glucose residues. Methylation analysis revealed the presence of a highly branched arabinoxylan with a significant amount of terminal arabinopyranosyl units and an arabinogalactan type I pectin. Periodate oxidation studies supported the results of methylation analysis.  相似文献   
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Laboratory bioassay was conducted to establish the biocontrol potentiality of naiads (aquatic nymphal stage) of Rhodothemis rufa (Rambur, 1842) against larvae of Culex quinquefasciatus, a common vector of filariasis in Tropical countries. From the study, it was noticed that in laboratory condition, the rate of predation of males of R. rufa was higher than that of females of almost same size and same species. The results of the present study revealed that both sexes displayed a density-dependent decelerating type-II functional response as the logistic regression estimated a significant negative linear parameter (P1 value of ?0.330 and ?0.151 for males and females, respectively). Attack rate was almost similar for both sexes (0.082); however, handling time is less in males (0.62 min) than in females (0.852 min). The predator species usually coexist in the same aquatic habitat to that of mosquito larvae and can be effectively used in field condition to reduce the larval densities of mosquitoes in temporary or permanent aquatic water bodies.  相似文献   
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In this study, we developed a simple, low-cost, and rapid flow through immunogold assay (FIA) for detection of Aeromonas hydrophila in fish tissues and validated. The developed assay relied on colloidal gold conjugated highly specific monoclonal antibody (B8E3) against 20 kDa protein of A. hydrophila. The assay can be completed within 5 min including antigen (sample) preparation and exhibited no cross-reaction with other major aquatic pathogens such as Vibrio anguillarum, V. parahaemolyticus, V. harveyi, white spot syndrome virus (WSSV), and Aphanomyces invadans. A wider range of Aeromonas species were tested including A. caviae, A. dhakensis, A. sobria, A. veronii, A. culicicola, A. sharmana, and A. schubertii and no cross-reactivity could be observed. The developed technique FIA could detect 103 CFU/ml of aeromonad cells. In addition, the FIA is as sensitive as the first-step polymerase chain reaction (PCR) and 1000 times sensitive than the immunodot blot method. The validation study revealed the accuracy of FIA by comparing with the standard first-step PCR. The proposed FIA is low-cost, easy-to-use, and compatible for field-level analysis of A. hydrophila in fish tissues. Our developed FIA could successfully detect A. hydrophila at early stage of the infection and could be easily availed by farmers, fish producers, non-technicians, and technicians in the aquaculture industry.  相似文献   
119.
Groundnut rust (Puccinia arachidis Speg.) is an important air borne pathogen, which causes substantial losses in groundnut yield and quality. Although large numbers of accessions were identified as rust resistant in wild, interspecific derivative and cultivated groundnut species, transfer of resistance to well-adapted cultivars is limited due to linkage drag, which worsens yield potential and market acceptance. A F2 mapping population comprising 117 individuals was developed from a cross between the rust resistant parent VG 9514 and rust susceptible parent TAG 24. Rust resistance was governed by single dominant gene in this cross. We identified 11 (out of 160) RAPD primers that exhibited polymorphism between these two parents. Using a modified bulk segregant analysis, primer J7 (5′CCTCTCGACA3′) produced a single coupling phase marker (J71350) and a repulsion phase marker (J71300) linked to rust resistance. Screening of the entire F2 population using primer J7 revealed that the coupling phase marker J71350 was linked with the rust resistance gene at a distance of 18.5 cM. On the other hand, the repulsion phase marker J71300 was completely linked with rust resistance. Additionally, both J71300 (P = 0.00075) and J71350 (P < 0.00001) were significantly associated with the rust resistance. Marker J71300 identified all homozygous rust resistant genotypes in the F2 population and was present in all the eight susceptible genotypes tested for validation. Thus, J71300 should be applicable for marker-assisted selection (MAS) in the groundnut rust resistance breeding programme in India. To the best of our knowledge this is the first report on the identification of RAPD markers linked to rust resistance in groundnut.  相似文献   
120.
Botrytis grey mould (BGM) is the major constraint to chickpea production in Bangladesh and is considered primarily responsible for that country's recent drastic decrease in chickpea production. There is no substantial host plant resistance to BGM in current chickpea cultivars, but component studies have developed various agronomic options to manage the disease. These include reduced seed rate, delayed sowing and thinning of plants to ensure an open canopy, and need-based foliar application of fungicide. These components were combined with other agronomic requirements for the target region, such as application of phosphate fertilizer, pest management measures against chickpea pod borer, and fungicidal seed treatment against collar rot. The resultant integrated crop management (ICM) package was compared with normal farmer practice (FP) for chickpea cultivation in farmer-managed, operational scale plots at 100 locations across five districts in western Bangladesh in the 2002–2003 and 2003–2004 seasons. Grain yields in ICM plots were generally 15–50% higher than in FP in both seasons. Conduct of these on-farm evaluations in two additional districts in 2004–2005 gave similar results. In 2004–2005, 505 farmer-managed demonstrations were conducted in the five original districts, giving a 5–104% yield advantage (district means) of ICM over FP. In 2005–2006, 642 demonstrations were conducted across the eight districts giving district-wise yield advantages of 27–70%. Effective implementation of BGM management practices by participating farmers demonstrated that remunerative and reliable chickpea yields could be obtained in this BGM-prone environment. The ICM strategy evolved has relevance to other chickpea growing regions prone to BGM in South Asia, Australia and the Americas. Studies are now required on the adoption of components of the ICM package, and the underlying reasons, to identify any adoption constraints and thus guide further promotion of chickpea cultivation.  相似文献   
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