Purification of anaphase promoting complex/cyclosome from goldfish oocytes     

T. Tokumoto  M. Tokumoto  M. Ishimatsu  R. Horiguchi  Y. Nagahama  K. Ishikawa 《Fish physiology and biochemistry》2003,28(1-4):371-371

Destruction of cyclin B is required for exit from mitosis and meiosis. A cyclin-specific ubiquitinating system, including anaphase-promoting complex/cyclosome (APC/C) is thought to be responsible for cyclin B destruction. To learn more about the molecular mechanism of cyclin B degradation, a molecular study of the ubiquitinating system in goldfish has been undertaken. For biochemical preparation of APC/C, we first conducted the cloning, sequencing and expression analysis of goldfish, Carassius auratus, cdc27 that encodes a subunit of APC/C from goldfish ovary. The deduced amino acid sequence is highly homologous to cdc27 from other species. Then recombinant goldfish Cdc27CT (C-terminal half of Cdc27) was expressed in Escherichia coli, and an antibody was raised against purified recombinant protein. Polyclonal antiserum cross-reactive with Cdc27 was obtained. By the assay using the antibody, APC/C was purified by column chromatographs.  相似文献   

The Yangtze River dolphin or baiji (Lipotes vexillifer): population status and conservation issues in the Yangtze River,China     

X. ZHANG  D. WANG  R. LIU  Z. WEI  Y. HUA  Y. WANG  Z. CHEN  L. WANG 《水产资源保护:海洋与淡水生态系统》2003,13(1):51-64

• 1. Baiji were sighted 17 times during three recent simultaneous multi‐vessel surveys in the Yangtze River, China (November 4–10, 1997; December 4–9, 1998; October 31–November 5, 1999). There were 11 sightings in 1997 (consisting of 17 animals), five in 1998 (seven animals), and two in 1999 (four animals). It was concluded that 13 individuals could be considered as a minimum number of the baiji currently in the Yangtze River.
• 2. An annual rate of population decrease was roughly estimated as 10%. From the body sizes observed, the proportions of old, adult and immature individuals were approximately estimated at 57, 26, and 17% respectively.
• 3. Baiji showed a significant attraction to confluences and sand bars with large eddies. The present distribution range of the baiji is less than 1400 km in length in the Yangtze main river. Distances between the two nearest groups of baiji appear to be increasing.
• 4. Two typical sightings are described, in which surfacing and movements of baiji were recorded. Baiji were often found swimming together with finless porpoise. In the surveys they occurred in the same group in 63% of occurrences. Interactions between baiji and finless porpoise are described and discussed.
• 5. Human activities are the main threats to the baiji. Illegal electrical fishing accounted for 40% of known mortalities during the 1990s. Engineering explosions for maintaining navigation channels have become another main cause of baiji deaths. The last hope of saving the species may be to translocate the remaining baiji into a semi‐captive reserve, known as the ‘Baiji Semi‐natural Reserve’.

Copyright © 2003 John Wiley & Sons, Ltd.  相似文献   

Polymerase chain reaction (PCR) amplification of bacilliform virus (RV-PJ) DNA in Penaeus japonicus Bate and systemic ectodermal and mesodermal baculovirus (SEMBV) DNA in Penaeus monodon Fabricius   总被引：3，自引：0，他引：3  

Y Takahashi  T Itami  M Maeda  N Suzuki  J Kasornchandra  K Supamattaya  R Khongpradit  S Boonyaratpalin  M Kondo  K Kawai  R Kusuda  I Hirono  T Aoki 《Journal of fish diseases》1996,19(5):399-403

  相似文献   

Reducing the potential for processing contaminant formation in cereal products     

Tanya Y. Curtis  Jennifer Postles  Nigel G. Halford 《Journal of Cereal Science》2014

Processing contaminants may be defined as substances that are produced in a food when it is cooked or processed, are not present or are present at much lower concentrations in the raw, unprocessed food, and are undesirable either because they have an adverse effect on product quality or because they are potentially harmful. The presence of very low levels of processing contaminants in common foods is becoming an increasingly important issue for the food industry, as developments in analytical techniques and equipment bring foods under closer and closer scrutiny. This review considers the formation of lipid oxidation products, hydrogenation of polyunsaturated fatty acids to prevent lipid oxidation and the associated risk of trans fatty acid formation. The formation of acrylamide in the Maillard reaction is described, as well as the genetic and agronomic approaches being taken to reduce the acrylamide-forming potential of cereal grain. The multiple routes for the formation of furan and associated chemicals, including hydroxymethylfurfuryl, are also described. The evolving regulatory and public perception situations for these processing contaminants and their implications for the cereal supply chain are discussed, emphasising the need for cereal breeders to engage with the contaminants issue.  相似文献   

Plant growth and survival of five introduced and two native/naturalized perennial grass genotypes exposed to two defoliation managements in arid Argentina     

Y. A. Torres  C. A. Busso  O. A. Montenegro  L. Ithurrart  H. D. Giorgetti  G. D. Rodríguez  R. E. Brevedan  O. A. Fernández  M. M. Mujica  S. S. Baioni  L. Entío  M. Fioretti  G. Tucat 《Grass and Forage Science》2014,69(4):580-595

The field performance of the native Pappophorum vaginatum, the naturalized Eragrostis curvula and various cultivars of the introduced Achnatherum hymenoides and Leymus cinereus was evaluated as potential forage resources in rangelands of arid Argentina during the warm seasons of 2007/2008 and 2008/2009. Plants of these grass species, obtained from seeds, were transplanted to the field in 2006, when they were 1 year old. During the study years, there were two defoliation managements: plants of all study genotypes either remained undefoliated (controls) or were defoliated twice a year during spring at 5 cm stubble height. Despite tiller number being lower (P < 0·05) on defoliated than on undefoliated plants, and total leaf length per unit basal area being similar (P > 0·05) between defoliation managements by mid‐spring, there were no differences (P > 0·05) in dry weight production between defoliated and undefoliated plants in all genotypes at the end of the second growing season. Plants of one or more of the introduced genotypes showed a similar (P > 0·05) or greater (P < 0·05), but not lower, tiller number per plant and per square centimetre, daughter tiller production, total leaf length and dry weight production per unit basal area than the native species at the end of the first and/or second growing seasons. These morphological variables were similar (P > 0·05) or greater (P < 0·05) in the native than in the naturalized genotype. Plant survival, however, was lower (P < 0·05, overall mean = 20%) in the introduced than in the native (>70%) or naturalized (>39%) genotypes at the end of the first or second growing seasons.  相似文献   

Fabrication of electromagnetic shielding polyester fabrics with carboxymethyl chitosan-palladium complexes activation     

Y. Shen  H. F. Zhang  L. M. Wang  L. H. Xu  Y. Ding 《Fibers and Polymers》2014,15(7):1414-1421

Electromagnetic shielding polyester fabrics were prepared using carboxymethyl chitosan-palladium (CMCS-Pd) complexes as activation solution, followed by electroless nickel plating. CMCS-Pd complexes were prepared by the complexing adsorption between CMCS and Pd²⁺. The effects of reaction time and pH value on the adsorption of Pd²⁺ by CMCS were discussed. The maximum adsorption capacity was calculated as 4.27 mmol/g. CMCS-Pd complexes were characterized by ultraviolet (UV) spectrophotometer and Fourier transform-infrared (FTIR) spectroscopy. The induction time of electroless plating decreased gradually with the increase of Pd²⁺ concentration. The lowest surface resistance 125 mΩ/sq of the treated polyester fabric was obtained when Pd²⁺ concentration in CMCS-Pd complex was 1.5 g/l. The prepared polyester fabrics had excellent electromagnetic shielding effectiveness (SE) of 40–60 dB. The treated polyester fabrics were also characterized by scanning electron microscopy (SEM). Results showed that CMCS-Pd was effective to form an active catalyzed layer on polyester substrate and the 1.5 g/l Pd²⁺ was sufficient to initiate electroless nickel plating reaction. The CMCS-Pd complex activation and electroless nickel plating treatment caused small changes in the polyester fabrics’ tensile strength and air permeability.  相似文献   

Composition of Typical Soil Minerals and Quantitative Analysis for Influence of Iron and Manganese Forms on Purple Soil Color in Northeastern Sichuan,China     

Liu  Y. F.  Liu  Z. L.  Rao  H. Y.  Wan  X.  Li  L. H.  Sun  S. Y.  Che  M. X.  Liu  M. X. 《Eurasian Soil Science》2022,55(6):781-789

Eurasian Soil Science - Eight soil samples from the Jurassic Shaximiao Formation (J2s) in the Sichuan Basin, China, were taken as the research object to explore the correlation and quantitative...  相似文献   

Combining remotely sensed surface energy fluxes and GIS analysis of groundwater parameters for irrigation system assessment     

T. K. Alexandridis  A. Panagopoulos  G. Galanis  I. Alexiou  I. Cherif  Y. Chemin  E. Stavrinos  G. Bilas  G. C. Zalidis 《Irrigation Science》2014,32(2):127-140

Despite being necessary for effective water management, the assessment of an irrigation system requires a large amount of input data for the estimation of related parameters and indicators, which are seldom measured in a regular and reliable manner. In this work, spatially distributed surface energy balance fluxes and geographical information systems analysis of multiple groundwater parameters were used to estimate water availability, supply, and demand, in order to calculate water-accounting indicators. This methodology was used to evaluate the performance of an irrigation system in the Pinios river basin (Greece) at two selected years of high and low water availability. Time series of archived satellite images and groundwater measurements have been used for past years to support comparative analyses, due to the limited availability of actual water measurements. The resulting maps from the proposed methodology show that the performance of the irrigation system varied across space and time due to differences in its characteristics and changes in its operation, driven by fluctuation of water availability and the response of stakeholders to water depletion. Irrigation districts with unsustainable water management were identified and, together with those with slow and/or limited groundwater recharge, were brought to the attention of water managers. The observed differences in the system operation between the wet and dry years were attributed not only to the hydrological conditions of each year, but also to the changing behaviour of farmers and the improvement actions of the water managers.  相似文献   

Fisheries of two tropical lagoons in Ghana, West Africa     

H. R. Dankwa  J. M. Shenker  & J. Lin  P. K. Ofori-Danson  Y. Ntiamoa-Baidu 《Fisheries Management and Ecology》2004,11(6):379-386

Abstract  The fisheries of two coastal lagoons, Keta and Songor, were studied as part of Ghana Coastal Wetlands Management Project (GCWMP) aimed at sustainable exploitation of wetland resources. Fish samples were obtained with seine nets and cast net as well as from local fishermen. Water quality parameters (pH, dissolved oxygen, temperature and turbidity) were similar in the two lagoons, except for salinity, which was significantly different ( P  < 0.001). Despite their close geographical proximity, the two lagoons supported different fish assemblages with the blackchin tilapia, Sarotherodon melanotheron Rüppell, and the redchin tilapia, Tilapia guineensis (Bleeker), being the most important commercial fishes in both lagoons. The number of individuals for each species in Songor Lagoon were far more abundant, with densities several orders of magnitude higher than in Keta Lagoon. However, both species were significantly larger ( P  < 0.01) in the latter [15–121 and 25–157 mm standard length (SL)] than in the former lagoon (30–102 and 15–95 mm SL) for S. melanotheron and T. guineensis respectively. Over-fishing, use of small-size mesh nets, limited mixing of marine and fresh water were some of the factors limiting fish production in both lagoons.  相似文献   

Digestive enzyme activity during early larval development of the Cuban gar Atractosteus tristoechus   总被引：1，自引：0，他引：1  

Y. Comabella  R. Mendoza  C. Aguilera  O. Carrillo  A. Hurtado  T. García-Galano 《Fish physiology and biochemistry》2006,32(2):147-157

The ontogenesis of digestive enzymes (proteases, amylases, lipases, and phosphatases) in Cuban gar Atractosteus tristoechus was determined in larvae between 5 and 18 days after hatching (DAH). Variations in specific activities of most enzymes were related to the transition from endogenous to exogenous feeding and to the transition from the larval to the juvenile stage. Alkaline protease activity was not detected until 8 DAH in contrast to acid protease activity, which was quantifiable at 5 DAH. Acid protease activity was consistently higher than alkaline protease activity, indicating the presence of a functional stomach in the early stages of larval development. The acid protease activities of larvae and adults were compared by means of zymogram analysis. Four acid protease bands were found in adults (two more than in larvae). This result is the first time that more than one band of acid proteolytic activity has been found in Lepisosteidae. High lipase activity indicated the importance of lipid utilization, particularly during yolk-sac absorption. In contrast to the other enzymes studied, amylase activity was consistently low, probably due to the strictly carnivorous diet of gar larvae and their low capacity for carbohydrate digestion. High activities of aminopeptidase and acid and alkaline phosphatases suggest intestinal absorption. This result, together with the existence of a short gut and a lower proteolytic activity in the intestine than in the stomach, suggest that most of the proteolytic activity takes place in the stomach, while the primary function of the intestine is nutrient uptake.  相似文献