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Júlia Vergara-Alert Núria Busquets Maria Ballester Aida J Chaves Raquel Rivas Roser Dolz Zhongfang Wang Stephan Pleschka Natàlia Majó Fernando Rodríguez Ayub Darji 《Veterinary research》2014,45(1):7
Some outbreaks involving highly pathogenic avian influenza viruses (HPAIV) of subtypes H5 and H7 were caused by avian-to-human transmissions. In nature, different influenza A viruses can reassort leading to new viruses with new characteristics. We decided to investigate the impact that the NS-segment of H5 HPAIV would have on viral pathogenicity of a classical avian H7 HPAIV in poultry, a natural host. We focussed this study based on our previous work that demonstrated that single reassortment of the NS-segment from an H5 HPAIV into an H7 HPAIV changes the ability of the virus to replicate in mammalian hosts. Our present data show that two different H7-viruses containing an NS-segment from H5–types (FPV NS GD or FPV NS VN) show an overall highly pathogenic phenotype compared with the wild type H7–virus (FPV), as characterized by higher viral shedding and earlier manifestation of clinical signs. Correlating with the latter, higher amounts of IFN-β mRNA were detected in the blood of NS-reassortant infected birds, 48 h post-infection (pi). Although lymphopenia was detected in chickens from all AIV-infected groups, also 48 h pi those animals challenged with NS-reassortant viruses showed an increase of peripheral monocyte/macrophage-like cells expressing high levels of IL-1β, as determined by flow cytometry. Taken together, these findings highlight the importance of the NS-segment in viral pathogenicity which is directly involved in triggering antiviral and pro-inflammatory cytokines found during HPAIV pathogenesis in chickens. 相似文献
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de Melo Ocarino N Fukushima FB de Matos Gomes A Bueno DF de Oliveira TS Serakides R 《Journal of Feline Medicine and Surgery》2006,8(5):345-348
This report describes the first case of idiopathic hypertrophic osteopathy (HO) in a cat. No causes for the bone pathology were found following evaluation of the physical and laboratory examinations (complete blood count, albumin, creatinine, urea, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase and gamma-glutamyltransferase and urinalysis), and after histopathological evaluation of organs at necropsy. Based on the radiographic, clinical and anatomopathological findings, idiopathic HO was diagnosed. 相似文献
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Thomas Bartels Dr. Rer. Nat. Jürgen Brinkmeier Dr. Med. Vet. Susanne Portmann Med. Vet. Ulrich Baulain Dr. sc. Agr. Axel Zinke Dr. Med. Vet. Maria-Elisabeth Krautwald-Junghanns Prof. Dr. Med. Vet. Alois Boos PD Dr. Med. Vet. Petra Wolf Dr. Med. Vet. Norbert Kummerfeld Dr. Med. Vet 《Veterinary radiology & ultrasound》2001,42(3):254-258
The crested breed of domestic duck (Anas platyrhynchos f. dom.) has been described as a variety which has high pre- and postnatal mortalities, malformations in skull and brain anatomy, and several central nervous deficiencies. In addition, intracranial tissue accumulations have been diagnosed in purebred Crested ducks. The incidence, heredity and inheritance of these accumulations as well as their pathogenesis are still generally unknown. The aims of this study were to examine the head of Crested ducks, plain-headed duck breeds, and their crossbreeding relating to the incidence of intracranial alterations. These examinations were performed using magnetic resonance imaging. We found a high incidence of intracranial tissue accumulations in domestic ducks with feather crests. Creasted ducks had more intracranial tissue deposits than plain-headed ducks (p < 0.001). In the present study a correlation between the volume of the crest cushion and the volume of the intracranial tissue deposit could not be found (r = 0.014). Some of the Crested ducks had encephaloceles in addition to the crest cushion. 相似文献
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Bertran K Pérez-Ramírez E Busquets N Dolz R Ramis A Darji A Abad FX Valle R Chaves A Vergara-Alert J Barral M Höfle U Majó N 《Veterinary research》2011,42(1):24
ABSTRACT: An experimental infection with highly pathogenic avian influenza virus (HPAIV) and low pathogenic avian influenza virus (LPAIV) was carried out in red-legged partridges (Alectoris rufa) in order to study clinical signs, gross and microscopic lesions, and viral distribution in tissues and viral shedding. Birds were infected with a HPAIV subtype H7N1 (A/Chicken/Italy/5093/1999) and a LPAIV subtype H7N9 (A/Anas crecca/Spain/1460/2008). Uninoculated birds were included as contacts in both groups. In HPAIV infected birds, the first clinical signs were observed at 3 dpi, and mortality started at 4 dpi, reaching 100% at 8 dpi. The presence of viral antigen in tissues and viral shedding were confirmed by immunohistochemistry and quantitative real time RT-PCR (qRRT-PCR), respectively, in all birds infected with HPAIV. However, neither clinical signs nor histopathological findings were observed in LPAIV infected partridges. In addition, only short-term viral shedding together with seroconversion was detected in some LPAIV inoculated animals. The present study demonstrates that the red-legged partridge is highly susceptible to the H7N1 HPAIV strain, causing severe disease, mortality and abundant viral shedding and thus contributing to the spread of a potential local outbreak of this virus. In contrast, our results concerning H7N9 LPAIV suggest that the red-legged partridge is not a reservoir species for this virus. 相似文献
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Carla Verônica Corrêa Aline Mendes de Sousa Gouveia Natália de Brito Lima Lanna Ana Emília Barbosa Tavares Veridiana Zocoler Mendonça Felipe Girotto Campos 《Journal of plant nutrition》2013,36(16):2048-2056
AbstractThe current study aimed to evaluate the effect of split application of potassium (K) fertilizer on the production and quality of sweet potatoes. Ten treatments were evaluated in factorial design (3?×?3?+?1): three doses of potassium (60, 90, and 120?kg K2O ha?1); three application types (100% applied at planting; 50% applied at planting and 50% was given as topdressing; 25% applied at planting and 75% was given as topdressing) and one control (without the application of K2O either at planting or topdressing). The experiment used a randomized complete block design and four repetitions. The following parameters were studied: root number; root fresh matter per plant; total dry matter; yield; total macronutrient extraction; sugar content of starch; and commercial starch. The production characteristics of the roots presented a significant interaction between the factors doses of potassium and application types (parcelaments). Finally, better results were obtained when potassium was applied 50% at planting and 50% as topdressing. 相似文献
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LR Mariutti DM Pereira AZ Mercadante P Valentão N Teixeira PB Andrade 《Marine drugs》2012,10(7):1498-1510
In this study, the carotenoid profile of the echinoderm Marthasterias glacialis L. was established using HPLC-DAD-APCI-MS/MS equipped with a C(30) column. This approach rendered the identification of 20 compounds, eight of them reported for the first time in this marine organism. Differentiation of carotenoid isomers was also achieved. 相似文献
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Silva JF Boeloni JN Cima AM Serakides R Ocarino NM 《Journal of Feline Medicine and Surgery》2012,14(6):424-427
A rare case of bilateral fusion of a supernumerary kidney was found during the necropsy of a female, 8-year-old, mixed breed cat that died as a result of azotemia and chronic enteritis. Apart from enteritis, necropsy revealed four kidneys, two in the sublumbar left region and two in the sublumbar right region, with cortical and medullary regions well individualized and independent; however, the pelvis was partially fused, giving rise to a single ureter. The kidneys were small, whitish and firm, with irregular surfaces. Microscopically, all kidneys displayed normal renal glomeruli and tubules among the immature renal glomeruli and tubules with characteristics of hypoplasia. Foci of glomerulosclerosis, nephrocalcinosis and interstitial fibrosis were also observed. 相似文献
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Cruz L Teixeira N Silva AM Mateus N Borges J de Freitas V 《Journal of agricultural and food chemistry》2008,56(22):10980-10987
Reactions between malvidin-3-glucoside (mv3glc) and 8-vinylcatechin were carried out to synthesize pyranomv3glc-(+)-catechin pigment and to study the formation of intermediates. A rapid decrease of mv3glc content concomitant with the formation of more complex structures such as mv3glc-vinylcatechin [precursor of pyranomv3glc-(+)-catechin pigment] and mv3glc-divinylcatechin was observed. On the other hand, 8-vinylcatechin undergoes acid-catalyzed dimerization in model wine solution, giving rise to 8-vinylcatechin dimers. These compounds were also found in the reaction between mv3glc and (+)-catechin mediated by acetaldehyde, which provides evidence for the formation of 8-vinylcatechin and its involvement in the formation of pyranoanthocyanins in aged red wines. 相似文献