Detection of Leishmania infantum in captive wolves from Southwestern Europe     

Sastre N  Francino O  Ramírez O  Enseñat C  Sánchez A  Altet L 《Veterinary parasitology》2008,158(1-2):117-120

The aim of the present study was to determine the prevalence of Leishmania infantum infection in a wild reservoir host (Canis lupus) throughout an endemic area for the disease (Southern Europe). For that reason, the serum and peripheral blood samples of 33 captive wolves from the European Breeding of Endangered Species Programme (EEP) were analyzed using the enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR (qPCR). L. infantum was detected in three samples from Central Portugal and Central and Northern Spain. Even though L. infantum infection in positive samples was low, surveillance of zoonotic leishmaniosis in this population is recommended as the parasite load could be higher in other tissues due to parasite tropism and most of the EEP institutions studied are located in endemic areas for canine leishmaniosis in Europe.  相似文献   

Echinochrome A Treatment Alleviates Atopic Dermatitis-like Skin Lesions in NC/Nga Mice via IL-4 and IL-13 Suppression     

Hyeong Rok Yun  Sang Woo Ahn  Bomin Seol  Elena A. Vasileva  Natalia P. Mishchenko  Sergey A. Fedoreyev  Valentin A. Stonik  Jin Han  Kyung Soo Ko  Byoung Doo Rhee  Jung Eun Seol  Hyoung Kyu Kim 《Marine drugs》2021,19(11)

Atopic dermatitis (AD) is a chronic inflammatory skin disease in which skin barrier dysfunction leads to dryness, pruritus, and erythematous lesions. AD is triggered by immune imbalance and oxidative stress. Echinochrome A (Ech A), a natural pigment isolated from sea urchins, exerts antioxidant and beneficial effects in various inflammatory disease models. In the present study, we tested whether Ech A treatment alleviated AD-like skin lesions. We examined the anti-inflammatory effect of Ech A on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like lesions in an NC/Nga mouse model. AD-like skin symptoms were induced by treatment with 1% DNCB for 1 week and 0.4% DNCB for 5 weeks in NC/Nga mice. The results showed that Ech A alleviated AD clinical symptoms, such as edema, erythema, and dryness. Treatment with Ech A induced the recovery of epidermis skin lesions as observed histologically. Tewameter^® and Corneometer^® measurements indicated that Ech A treatment reduced transepidermal water loss and improved stratum corneum hydration, respectively. Ech A treatment also inhibited inflammatory-response-induced mast cell infiltration in AD-like skin lesions and suppressed the expression of proinflammatory cytokines, such as interferon-γ, interleukin-4, and interleukin-13. Collectively, these results suggest that Ech A may be beneficial for treating AD owing to its anti-inflammatory effects.  相似文献   

Identification of New Betalains in Separated Betacyanin and Betaxanthin Fractions from Ulluco (Ullucus tuberosus Caldas) by HPLC-DAD-ESI-MS     

Mosquera  Natalia  Cejudo-Bastante  María Jesús  Heredia  Francisco J.  Hurtado  Nelson 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(3):434-440

Plant Foods for Human Nutrition - An improved methodology of achieving an accurate separation of pure fractions of betacyanins and betaxanthins from Ullucus tuberosus Caldas has been carried out....  相似文献   

Determination and Comparison of the Lipid Profile and Sodium Content of Gluten-Free and Gluten-Containing Breads from the Spanish Market     

Tres  Alba  Tarnovska  Natalia  Varona  Elisa  Quintanilla-Casas  Beatriz  Vichi  Stefania  Gibert  Anna  Vilchez  Elisenda  Guardiola  Francesc 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(3):344-354

Plant Foods for Human Nutrition - The objective is to verify if gluten-free (GF) and gluten-containing (G) breads differ in their sodium content and lipid profile. Samples of GF...  相似文献   

New invertebrate species as potential CyHV-3 reservoirs: A case study of common carp mortalities in hyperthermal conditions     

Remigiusz Panicz  Piotr Eljasik  Natalia Śmietana  Jacek Sadowski  Marcin Biernaczyk 《Journal of fish diseases》2020,43(7):821-824

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Climate variability and trends in the reproductive habitat of the critically endangered hooded grebe     

Julio L. Lancelotti  Natalia L. Pessacg  Ignacio C. Roesler  Miguel A. Pascual 《水产资源保护:海洋与淡水生态系统》2020,30(3):554-564

1. The population of the critically endangered hooded grebe (Podiceps gallardoi) has decreased drastically during recent decades. The effects of climate change on their reproductive area (Southern Patagonia) have been proposed as one of the potential causes of their critical situation. However, there is much uncertainty about climate variability and climate trends at local scales in Patagonia, and their effects on the hooded grebe habitat.
2. Variability and trends in temperature, precipitation, and wind from 1960 to 2016 in one of the most important reproductive areas of hooded grebe – the Lake Strobel plateau – were analysed. A Landsat satellite image series (1973–2016) was used to evaluate changes in lake area in the region.
3. Generalized linear models were used to analyse the relationship between the variation in lake area and climatic variables, including the Antarctic Oscillation Mode (AAO) index, which is the most important year to year pattern in climate variability at high latitudes of the Southern Hemisphere.
4. High inter‐annual variability and increasing coefficients of variation in precipitation and wind were found, as well as a significant positive trend in temperature. Total lake area, as well as the area specifically used by hooded grebe to reproduce, decreased significantly over time, with high inter‐annual variability. Variation in lake area was significantly correlated with variation in precipitation, and with the AAO index.
5. The loss of reproductive habitat may force the hooded grebe to use sub‐optimal environments, such as lakes with lower food supply, lower macrophyte cover, and less protection from wind – a potential major handicap for this declining species.
6. Information on lake variation in response to climatic variability could be used to guide hooded grebe conservation strategies, directing more resources and special efforts to those reproductive lakes that are more resilient to the effects of climate fluctuations.

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Carbohydrate tolerance in the fruit‐eating fish Piaractus mesopotamicus (Holmberg, 1887)          下载免费PDF全文

Leonardo Susumu Takahashi  Natalia Ha  Mayara Moura Pereira  Jaqueline Dalbello Biller‐Takahashi  Elisabeth Criscuolo Urbinati 《Aquaculture Research》2018,49(3):1182-1188

Some fish species have a limited ability to metabolize dietary carbohydrates. An important tool for understanding carbohydrate metabolism is the application of the glucose tolerance test, which can be performed orally or intraperitoneally. To evaluate carbohydrate tolerance in the fruit‐eating fish pacu, two experiments were performed, one with oral administration by gavage of three carbohydrate types (glucose, fructose and starch, 2.0 g/kg body weight (BW)) and the other with intraperitoneal injection (IP) of glucose (500 mg/kg BW). Oral glucose resulted in an increase in plasma glucose 2 hr later with the peak at 4 hr (8.30 mmol/L), and return to baseline between 6 and 12 hr; starch administration promoted a peak after 4 hr (7.70 mmol/L), returning to the baseline at 6 hr. The administration of fructose promoted a moderate peak after 2 hr (5.71 mmol/L), and return to baseline for the time points that followed. Elevated serum cholesterol levels were observed 2 and 24 hr after administration of glucose and starch. Hepatic glycogen levels increased within 24 hr, regardless of the type of carbohydrate administered. IP glucose load resulted in a peak of plasma glucose 3 hr post injection (6.91 mmol/L), returning to baseline 6 hr later. There was a reduction in the concentration of triglycerides at 24 hr. The results demonstrate that pacu metabolize both oral (glucose or starch) and intraperitoneal (glucose) carbohydrate loads after 6 hr, suggesting good ability to deal with dietary carbohydrates.  相似文献   

Additive and interactive effects of functionally dissimilar soil organisms on a grassland plant community     

Natalia Ladygina  Frederic Henry  Robert Koller  Alia Rodriguez  Ilja Sonnemann  Susanne Wurst 《Soil biology & biochemistry》2010,42(12):2266-2275

The productivity and diversity of plant communities are affected by soil organisms such as arbuscular mycorrhizal fungi (AMF), root herbivores and decomposers. However, it is unknown how interactions between such functionally dissimilar soil organisms affect plant communities and whether the combined effects are additive or interactive. In a greenhouse experiment we investigated the individual and combined effects of AMF (five Glomus species), root herbivores (wireworms and nematodes) and decomposers (collembolans and enchytraeids) on the productivity and nutrient content of a model grassland plant community as well as on soil microbial biomass and community structure. The effects of the soil organisms on productivity (total plant biomass), total root biomass, grass and forb biomass, and nutrient uptake of the plant community were additive. AMF decreased, decomposers increased and root herbivores had no effect on productivity, but in combination the additive effects canceled each other out. AMF reduced total root biomass by 18%, but decomposers increased it by 25%, leading to no net effect on total root biomass in the combined treatments. Total shoot biomass was reduced by 14% by root herbivores and affected by an interaction between AMF and decomposers where decomposers had a positive impact on shoot growth only in presence of AMF. AMF increased the shoot biomass of forbs, but reduced the shoot biomass of grasses, while root herbivores only reduced the shoot biomass of grasses. Interactive effects of the soil organisms were detected on the shoot biomasses of Lotus corniculatus, Plantago lanceolata, and Agrostis capillaris. The C/N ratio of the plant community was affected by AMF.In soil, AMF promoted abundances of bacterial, actinomycete, saprophytic and AMF fatty acid markers. Decomposers alone decreased bacterial and actinomycete fatty acids abundances but when decomposers were interacting with herbivores those abundances were increased. Our results suggests that at higher resolutions, i.e. on the levels of individual plant species and the microbial community, interactive effects are common but do not affect the overall productivity and nutrient uptake of a grassland plant community, which is mainly affected by additive effects of functionally dissimilar soil organisms.  相似文献   

Impact odorants of different young white wines from the Canary Islands   总被引：5，自引：0，他引：5  

López R  Ortín N  Pérez-Trujillo JP  Cacho J  Ferreira V 《Journal of agricultural and food chemistry》2003,51(11):3419-3425

Five young monovarietal white wines from the Canary Islands made from Gual, Verdello, Marmajuelo, white Listán, and Malvasia grape cultivars were studied to determine the characteristics of their most important aromas and the differences among them. The study was carried out using gas chromatography-olfactometry (GC-O) to detect the potentially most important aroma compounds, which were then analyzed quantitatively by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. The strongest odorants in the GC-O experiments were similar in all cases, although significant differences in intensity between samples were noted. Calculation of the odor activity values (OAVs) showed that 3-mercaptohexyl acetate was the most active odorant in the Marmajuelo and Verdello wines, as were 3-methylbutyl acetate in the Gual wine, beta-damascenone in the Malvasia wine, and ethyl octanoate in the white Listán wine. However, the most important differences between varieties were caused by the three mercaptans (3-mercaptohexyl acetate, 3-mercaptohexanol, and 4-methyl-4-mercapto-2-pentanone) and the vinylphenols (4-vinylphenol and 2-methoxy-4-vinylphenol). The correlation between the olfactometric values and the OAVs was satisfactory in the cases when the compound eluted in the GC-O system was well isolated from other odorants and had aromatic importance and the OAVs for the different wines were sufficiently different.  相似文献   

An information retrieval system for VIR's herbarium, St. Petersburg, Russia, as a tool for cultivated plant research     

Natalia N. Luneva  Irena G. Chukhina  Elizaveta G. Lebedeva 《Genetic Resources and Crop Evolution》2000,47(2):147-152

The herbarium collection of the N.I. Vavilov Institute of Plant Industry (WIR) contains specimens of cultivated plants, their wild relatives and weedy plants and provides ample opportunities for research. It is a unique special herbarium collection and one of those rare collections having a card index (catalog). For the catalog to be functional, it should reflect the current changes in the taxa names. Only a computerized card file may meet this requirement. The creation of a database for VIR's herbarium was launched in 1995. VIR is carrying out a range of work necessary for providing a wider access to its herbarium and related information for the international scientific community.  相似文献