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71.

Purpose

The main objective of this study was to examine the potential of using hyperspectral image analysis for prediction of total carbon (TC), total nitrogen (TN) and their isotope composition (δ13C and δ15N) in forest leaf litterfall samples.

Materials and methods

Hyperspectral images were captured from ground litterfall samples of a natural forest in the spectral range of 400–1700 nm. A partial least-square regression model (PLSR) was used to correlate the relative reflectance spectra with TC, TN, δ13C and δ15N in the litterfall samples. The most important wavelengths were selected using β coefficient, and the final models were developed using the most important wavelengths. The models were, then, tested using an external validation set.

Results and discussion

The results showed that the data of TC and δ13C could not be fitted to the PLSR model, possibly due to small variations observed in the TC and δ13C data. The model, however, was fitted well to TN and δ15N. The cross-validation R2 cv of the models for TN and δ15N were 0.74 and 0.67 with the RMSEcv of 0.53% and 1.07‰, respectively. The external validation R2 ex of the prediction was 0.64 and 0.67, and the RMSEex was 0.53% and 1.19 ‰, for TN and δ15N, respectively. The ratio of performance to deviation (RPD) of the predictions was 1.48 and 1.53, respectively, for TN and δ15N, showing that the models were reliable for the prediction of TN and δ15N in new forest leaf litterfall samples.

Conclusions

The PLSR model was not successful in predicting TC and δ13C in forest leaf litterfall samples using hyperspectral data. The predictions of TN and δ15N values in the external litterfall samples were reliable, and PLSR can be used for future prediction.
  相似文献   
72.
Acid sulfate soils (ASS) are characterized by low pH, aluminum (Al), and iron (Fe) toxicity and are typically deficient in phosphate (PO4). The application of phosphorus (P) fertilizer could help reduce the level of exchangeable Al and Fe, thereby improving the rice growth and yield. Five levels of P (0, 20, 40, 60 and 80 kg phosphorus pentoxide (P2O5)/ha) were tested with rice varieties MTL560 in the wet season and MTL480 in the dry season. The optimum rate of P was 60 kg P2O5/ha for rice in the dry season and 80 kg P2O5/ha in the wet season. Soil testing showed at the start of the season that there was sufficient P in the soil. At the end of the season there was a reduction in soil Al and Fe in plots that had P rates above 40 kg P2O5/ha. It is therefore likely that P application reduced Al and Fe toxicity through precipitation and formation of Al-P and Fe-P compounds, which boasted yield, rather amending a soil P deficiency.  相似文献   
73.
Spherical biochar derived from saccharides (glucose, sucrose, and xylose) was prepared through two steps: pre-hydrothermal carbonization at 190 °C and calcination at low temperatures (200–325 °C). The spherical biochar was characterized by Brunauer–Emmett–Teller (BET) surface area analysis, Fourier transform infrared spectroscopy, zeta potential, scanning and transmission electron microscopies, and X-ray diffraction. The result indicated that the spherical biochar exhibited low S BET (15–22 m2/g), but abundant superficial active oxygen-containing functional groups. The spherical biochar possessed a negatively charged surface within solution pH 2.0–11. The adsorption process of Pb2+, Cu2+, and methylene green 5 (MG5) was strongly dependent on the solution pH and reached fast equilibrium at approximately 60 min. The maximum Langmuir adsorption capacity (Q°max) exhibited the following order: glucose-biochar > sucrose-biochar > xylose-biochar prepared at 300 °C. The selective adsorption order of glucose-biochar was Cu2+ (0.894 mmol/g) > Pb2+ (0.848 mmol/g) > MG5 (0.334 mmol/g). The electrostatic attraction played a determining role in the adsorption mechanism of pollutant cations. The adsorption of anionic dye (acid red 1) on the spherical biochar was negligible because of electrostatic repulsion. The spherical biochar can serve as a newer and promising adsorbent to remove toxic pollutant cations from water media.  相似文献   
74.
This research was conducted to investigate the effects of different medium compositions on physicochemical properties and the growth of two tomato varieties. Five treatments were applied, combining medium composition with rice husk ash (RHS) and coconut fiber (CF) with the following proportions: 1/3 Peat moss + 1/3 rice husk ash + 1/3 coconut fiber (T1); 1/3 Vermicompost + 1/3 rice husk ash + 1/3 coconut fiber (T2); 1/3 Cattle manure compost + 1/3 rice husk ash + 1/3 coconut fiber (T3); 1/3 Chicken manure compost + 1/3 rice husk ash + 1/3 coconut fiber (T4); 1/3 Hog manure compost + 1/3 rice husk ash + 1/3 coconut fiber (T5). The results demonstrated that between pH, electrical conductivity (EC) value, and nutrient content in the media, there was a significant difference that led to different concentrations of total macro- and micronutrients in the shoots of both varieties. In addition, the root weight, shoot weight, and root volume were affected by medium composition for two varieties in seedling stage. A mixture of cattle manures composted with RHS and CF under the rate (1:1:1 by volume), respectively, gave the highest value of germination rate, plant height, leaf number, and plant biomass. Medium composition significantly influenced the germination rate and elongation of seedlings, specifically for each tomato variety. Therefore, cattle manure compost as a medium supplement improves seedling quality and growth of the two tomato varieties.  相似文献   
75.
Reports of livestock infections with extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-E) are increasing. Based on interviews conducted over a 6-month period, we found that veterinarians in the Vietnamese province of Thai Binh prefer to prescribe colistin-based drugs (CBD) in chicken farms. We aimed to clarify whether CBD use selects for strains of colistin-resistant ESBL-E. With the cooperation of seven local households, we detected ESBL-E in chickens’ feces after treating chickens with CBD. Phylogenetic groupings and the presence of CTX-M/AmpC genes were determined, and the multi-antibiotic susceptibility of isolates was analyzed. Our results showed that ESBL-E presented in seven chickens’ feces from two households. Seventy-two percent of ESBL-E isolates harbored CTX-M9 and the phylogenetic group A; the colistin minimum inhibitory concentration (MIC) of all isolated ESBL-E ranged from 0.064 to 1 μg mL?1. Moreover, ESBL-E isolates were used to experimentally select for colistin resistance, and the effect of commercial CBD on ESBL-E was investigated. The results showed that an ESBL-E strain with a colistin MIC of 4 μg mL?1 was able to grow in media with CBD. Although CBD treatment was effective, in vitro experiments demonstrated that ESBL-E can easily acquire colistin resistance. Therefore, restrictions on colistin use are necessary to prevent the emergence of colistin-resistant bacteria.  相似文献   
76.
A field experiment was conducted to investigate the impact of simulated rat damage on grain yield in irrigated lowland rice in An Giang province, in the Mekong Delta of Vietnam. Rat damage was simulated by making a 45° cut at the base of the rice tillers about 3–5 cm above the soil surface. 0% (control), 5%, 10%, 25% and 50% of the tillers were removed at the tillering (25 days after sowing, DAS), panicle initiation (43 DAS), flowering (72 DAS) and ripening (87 DAS) stages. The total number of mature tillers, immature tillers and percent filled grains, the weight of 1000 grains and grain yields were recorded. The rice crop completely compensated by increased tillering and yield for tillers damaged up to 50% at the tillering stage. However, the damage and yield loss were highly variable. The ability of the rice crop to compensate at later stages progressively reduced. The implications for managing rat damage to rice crops are (1) rodent control activities should be applied before 10% of tillers are damaged at the tillering stage, and (2) a well balanced nitrogen supply during the tillering stage could potentially assist the rice plants to better compensate for rat damage.  相似文献   
77.
This study aims to analyze the economic feasibility of recirculating aquaculture systems (RAS) in pangasius farming in Vietnam. The study uses a capital budgeting approach and accounts for uncertainty in key parameters. Stochastic simulation is used to simulate the economic performance of medium and large farms operating with a traditional system or RAS. Data are obtained through structured surveys and a workshop in the Mekong River Delta. Results show that for large farms, net present value increases from an average of 589,000 USD/ha to 916,000 USD/ha after implementing RAS. Overall, the probability that RAS is a profitable investment is found to be 99% for both farm sizes. With RAS, the crucial parameters determining profitability are price, yield, costs of fingerling, feed, and initial investment. Findings on the robustness of the economic performance of RAS are useful to support public and private decision making towards increasing the sustainability of pangasius production.  相似文献   
78.
Random amplified polymorphism DNA (RAPD) and bulk segregant analysis (BSA) approaches were used to characterize the molecular marker linked to the Phytophthora infestans resistance gene Ph-3 in tomato. A total of 800 RAPD primers were screened. One RAPD marker UBC#602 was identified to be tightly linked to the Ph-3 gene. The marker was successfully converted into a co-dominant sequence characterized amplified region (SCAR) marker. The SCAR marker SCU602 was used to analyze 96 F2 progenies and fitted the expected 1:2:1 Mendelian segregation ratio. Forty one tomato inbred lines were screened using the SCAR marker in comparison with a reference marker linked to the Ph-3 gene and both markers gave the same results. SCU602 was further validated for association to resistance and its potential in MAS in 72 tomato lines and cultivars. The marker identified three genotypes harbouring the resistance allele. This SCAR marker can be used in breeding programs for the selection of the Ph-3 gene for Phytophthora infestans resistance.  相似文献   
79.
根据植物葡萄糖-6-磷酸脱氢酶(G6PDH,EC1.1.1.49)氨基酸保守区域,设计简并引物,采用RT-PCR技术克隆得到甜瓜属野生种Cucumis hystrix Chakr.(2n=24)G6PDH基因cDNA片段;随后基于该序列设计特异引物,PCR方法筛选野生种BAC文库,获得2个BAC阳性单克隆。测序后获得了G6PDH基因全序列及其上游启动子序列,GenBank登录号:JQ771576。序列分析显示:G6PDH基因全长约6.5 kb,由15个外显子和14个内含子组成;内含子序列均符合5’-gt-ag-3’结构。外显子拼接后获得了G6PDH基因的开放阅读框(ORF)序列,序列全长1 551 bp,编码516个氨基酸,与黄瓜基因组网站公布的G6PDH基因核苷酸序列和氨基酸序列同源性分别为98.71%和99.03%。野生种G6PDH氨基酸序列N端缺少转运肽序列,确定为野生种胞质G6PDH。氨基酸序列与烟草、马铃薯、荷兰芹、猕猴桃、拟南芥、大豆、小麦、玉米、葡萄、杨树等植物胞质G6PDH同源性高达77%以上。系统发育树分析发现,甜瓜属胞质G6PDH与茄科植物最先聚类,植物胞质G6PDH在分子进化水平上与物种进化相符。启动子结构分析表明:序列含有启动子基本元件TATA-box、CAAT-box,还含有丰富的光响应元件,与环境胁迫响应相关的不同顺式作用元件,促进高水平转录的5UTR Py-rich stretch元件和提高表达的CAT-box元件等。  相似文献   
80.
Intensive cropping, especially of rice, is considered to contribute to negative effects not only on soil chemical and biological properties but also on long-term grain yield. Appropriate crop rotation is often practiced as an alternative strategy to overcome the negative side effects of intensive cropping. Although soil microbial diversity and community structure have been shown to respond differently to altered agricultural management practices, little is known about possible links between crop rotation and grain yield on bacterial communities in rice paddy soil. In this study, we investigated the impact of specific rotational crops and compared it with intensive rice cultivation. The main crop rice (Oryza sativa) was rotated with maize (Zea mays) and mungbean (Phaseolus aureus) in different combinations in a system cultivating three crops per year. Soil bacterial communities were studied in two different cropping periods using pyrosequencing of the variable V4 region of the 16S rRNA. Our results showed that rotation with alternative crops increased rice yield by 24–46% depending on rotation structure and that bacterial community structure was altered in the presence of mungbean and/or maize compared to that in rice monoculture. In the crop rotation systems, composition, abundance, and diversity of soil bacterial communities were significantly different and higher than those in rice monoculture. Our results show that effects of crop rotation relate to changes in soil bacterial community structure suggesting that appropriate crop rotations provide a feasible practice to maintain the equilibrium in soil microbial environment for sustainable rice cultivation.  相似文献   
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