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711.
Households and wood processing businesses in the provinces of Son La, Dien Bien and Lai Chau in the Northwest of Vietnam were surveyed to determine patterns of local wood demand and supply, changes in species utilized over time, and whether timber from planted trees might substitute for species previously harvested from local natural forests. In each province, 5–6 sawmills and 4–6 villages representing 3 main ethnic groups were selected for investigation. Managers of selected sawmills and 3–4 randomly selected households in each village were interviewed using semi-structured questionnaires. There was high and uniform household demand for fuelwood in the surveyed villages, making fuelwood the dominant use, in terms of wood volume. Use of sawn timber for furniture, home repair and construction consumed less wood but required logs of acceptable species. Sawmills surveyed were small, with input capacity ranging from <15 to 500 m3 of logs per year, suggesting a total log requirement of about 30,000 m3/year for the 192 known wood processing businesses in the three provinces. Most sawmills still used wood from natural forests, sourced locally or imported from Laos. A trend of switching from prized timber species from natural forests, now effectively unavailable, to alternatives from natural and planted forests was reported by both sawmills and households. Some planted species grown on short-medium rotations appear able to substitute for local sawn timber requirements.  相似文献   
712.
A phytochemical fractionation of the methanol extract of the Morus alba leaves led to the isolation of eleven flavonoids (111). The structure of the new 3′-geranyl-3-prenyl-2′,4′,5,7-tetrahydroxyflavone (1) was elucidated by means of spectroscopic methods. The cytotoxicity of the isolated compounds against human cervical carcinoma HeLa, human breast carcinoma MCF-7, and human hepatocarcinoma Hep3B cells was evaluated. Of note, morusin (9) was the most potent with an IC50 value of 0.64 μM against HeLa cells.  相似文献   
713.
This field trial was conducted to determine whether 40 or 75 mg/kg of praziquantel is suitable for treatment of fishborne zoonotic trematodes (FZT) in naturally infected dogs (n=10) and cats (n=11). Three days after treatment all animals at either dose were negative for small trematode eggs. In two cats and one dog treated with 75 mg/kg, however, a few damaged eggs were found 3 days post-treatment; no small trematode eggs were seen in these animals at day 14 post-treatment. In addition, at the 75 mg dose, two cats and two dogs experienced vomiting or diminished appetite. Therefore a praziquantel dose of 40 mg/kg is suggested for treatment of FZT in dogs and cats.  相似文献   
714.
Edema disease (ED) is a common fatal disease in newly weaned piglets. To develop an effective control program for ED, we carried out a study to better understand the incidence and spread of the disease and the characteristics of the causative agent. In our study, 69 Escherichia coli strains, isolated from 92 piglets showing clinical signs of ED from 13 provinces in northern Vietnam, were positive for both the VT2e toxin and the F18 major fimbrial subunit gene fedA. Of these, 40 strains (58%) were positive for AIDA and 16 isolates carried one or more enterotoxins. Forty-six (67%) of the 69 VT2e+/F18+ E. coli isolates belonged to classical serotypes (O139:K82, O141: K85, O138:K81, and O149:K91) while the remaining strains did not belong to the common serotypes in pig. Seropathotype 0139:K82+/VT2e+/F18+/AIDA+ (21 isolates) was the most frequently detected ED-causing E. coli strain. High prevalence of resistance was observed to the common drugs of tetracycline, streptomycin, trimethoprim/sulfamethoxazole, amoxicillin/clavulanic acid, and spectinomycin. Multiple resistances were widely distributed with 84% of isolates resistant to five antibiotics. Sequence analysis demonstrated that the VT2e toxin is identical among E. coli strains causing ED in pig.  相似文献   
715.
Volatile insecticides, otherwise known as fumigants, have numerous uses in pest control and integrated pest management. Fumigants have been broadly applied in the protection of stored agricultural commodities and also in the elimination of structural pest infestations. However, fumigants have so far been mostly overlooked for use in management of public health pests such as flies and mosquitoes. This paper reports information on the development of a volatility bioassay for use in evaluating volatile insecticides against dipteran insects and on the use of this bioassay to compare the relative efficacy of 30 candidate compounds to Drosophila melanogaster Meig. The findings revealed seven highly effective insecticidal compounds, which include three heterobicyclics (menthofuran, thiophene and coumaran) and four formate esters [butyl, hexyl and heptyl formate and ethylene glycol diformate (EGDF)]. Ethyl formate, a compound previously identified as being a highly effective fumigant against coleopteran stored product pests, caused toxicity in Drosophila but was not as effective as other tested formates. Two volatile fumigant insecticides, dichlorvos (DDVP) and methyl isothiocyanate (MITC), were also tested as positive controls. Both of these volatile standards were significantly more toxic than the seven most effective of the novel compounds. Finally, regression analyses revealed that volatility is not entirely predictive of acute toxicity and that other structural features should be considered when designing and synthesizing volatile insecticidal compounds for future study.  相似文献   
716.
OBJECTIVE: To evaluate the effect of dietary fat and energy density on body weight gain, body composition, and total energy expenditure (TEE) in neutered and sexually intact cats. ANIMALS: 12 male and 12 female cats PROCEDURE: Male cats were castrated (castrated male [CM]) or underwent no surgical procedure (sexually intact male [IM]). Female cats underwent ovariectomy (spayed female [SF]) or laparotomy and ligation of both uterine tubes without ovary removal (sexually intact female [IF]). Cats were fed either the low-fat (LF) or high-fat (HF) diet for 26 weeks, with the final allocation consisting of 8 groups: IF-LF IF-HE SF-LF, SF-HF IM-LF, IM-HF, CM-LF, and CM-HF. Mean food intake for each group was recorded daily, and body weight was monitored weekly throughout the study. Body composition and TEE were measured before surgery in week 0 and at the end of the study (week 26) by isotope dilution (double-labelled water). RESULTS: N eutered cats gained significantly more body fat and body weight (53.80+/-5.79%) than sexually intact cats (27.11+/-5.79%) during the study. Body weight gain of neutered cats fed the HF diet was greater than those fed the LF diet. Following correction for body composition, TEE was similar in all groups and no pattern towards increased food intake was evident. CONCLUSIONS AND CLINICAL RELEVANCE: Weight gain in neutered cats was decreased by feeding an LF, low energy-dense diet. To prevent weight gain in cats after neutering, a suitable LF diet should be fed in carefully controlled meals rather than ad libitum.  相似文献   
717.
To determine the feasibility of preserving oocytes without freezing, we stored mouse oocytes in several media at different temperatures for one day. Confocal microscopy of the metaphase-II spindle in these stored oocytes revealed gross abnormalities in both the spindle and the arrangement of chromosomes. The abnormal spindles could not be rescued by transplanting the aged spindle-chromosome complex into a fresh enucleated oocyte. A diploid parthenogenetic development showed that some of the oocytes stored at room temperature could still develop into blastocysts (10-57%). However, oocytes stored in a refrigerator (5%) or incubator (0%) lost the potential almost entirely. Fertilization of room-temperature-preserved oocytes with fresh spermatozoa by ICSI or IVF resulted in, respectively, 4 and 10%, full-term births. These results suggest that when oocytes are stored at room temperature for one day, most have irreversible damage not only to their cytoplasm but also to the spindle. However, since at least a few percent of stored oocytes retained the potential for full-term development, it may be possible to overcome these problems and develop a simple method for preserving mammalian oocytes without freezing.  相似文献   
718.
The aim of the study was to investigate the effect on growth performance of the addition of 5% of either linseed, fish or coconut oil to the diet of growing pigs kept on small holdings in central Vietnam. The diets were fed on six different farms; there were three animals per treatment per farm. The farmers fed a base diet according to personal choice, but were instructed to use linseed, fish and coconut oil. The diets were fed to the pigs from 70 to 130 days of age. The diets with 5% linseed oil on average contained 2.4 g alpha-linolenic acid/MJ of metabolizable energy (ME) and the diets with 5% fish oil on average contained 0.06 and 0.75 g eicosapentaenoic and docosahexaenoic acid/MJ ME, respectively. The relative percentages of docosahexaenoic, eicosapentaenoic, alpha-linolenic and linoleic acid in adipose tissue were determined by the intake of the corresponding fatty acids. Dietary linseed oil vs. coconut oil significantly enhanced daily weight gain and vs. fish oil it also stimulated growth, but this effect just failed to reach statistical significance. It is concluded that extra intake of alpha-linolenic acid may stimulate growth in growing-finishing pigs, this effect being independent of the conversion of alpha-linolenic acid into eicosapentaenoic and docosahexaenoic acid.  相似文献   
719.
A young cat with signs of chronic rhinitis was evaluated for underlying anatomical, inflammatory, or infectious disease. Initial diagnostics were significant for the isolation of an unusual pathogen, Haemophilus species. Isolation using a human RapID NH system erroneously identified the isolate as H. segnis, a human pathogen. No database of veterinary pathogens (Haemophilus) are included in the system and animal pathogens will either be erroneously identified or yield a unique biocode not listed. Because of the unique nature of the pathogen we explored the possibility of immunosuppression as a contributory factor to infection. A variety of laboratory tests were employed to evaluate immune function. The clinical indications and utility of immune function testing are discussed. No immune dysfunction was identified.  相似文献   
720.
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