全文获取类型
收费全文 | 234篇 |
免费 | 13篇 |
国内免费 | 1篇 |
专业分类
林业 | 14篇 |
农学 | 4篇 |
基础科学 | 3篇 |
41篇 | |
综合类 | 23篇 |
农作物 | 9篇 |
水产渔业 | 35篇 |
畜牧兽医 | 91篇 |
园艺 | 8篇 |
植物保护 | 20篇 |
出版年
2022年 | 3篇 |
2021年 | 3篇 |
2020年 | 4篇 |
2019年 | 5篇 |
2018年 | 4篇 |
2017年 | 4篇 |
2016年 | 11篇 |
2015年 | 9篇 |
2014年 | 6篇 |
2013年 | 9篇 |
2012年 | 8篇 |
2011年 | 11篇 |
2010年 | 12篇 |
2009年 | 9篇 |
2008年 | 8篇 |
2007年 | 15篇 |
2006年 | 13篇 |
2005年 | 9篇 |
2004年 | 10篇 |
2003年 | 10篇 |
2002年 | 12篇 |
2001年 | 8篇 |
2000年 | 6篇 |
1999年 | 4篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 5篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 1篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1972年 | 1篇 |
1943年 | 2篇 |
1942年 | 1篇 |
排序方式: 共有248条查询结果,搜索用时 15 毫秒
111.
112.
D L McCaw G D Boon A E Jergens M R Kern M H Bowles J C Johnson 《Journal of the American Animal Hospital Association》2001,37(4):356-363
Clinically ill feline leukemia virus (FeLV)-infected cats, treated with Staphylococcus protein A (SPA) or oral interferon alpha (IFN), or both, were compared with cats treated with saline (SAL). Nine cats received SPA/SAL, nine received SPA/IFN, 10 received SAL/IFN, and eight received SAL/SAL. Twelve cats survived and completed the 100-week therapy. Significantly more owners of cats treated with SPA/SAL thought their cat's health improved during treatment compared to owners of cats treated with SAL/SAL (P=0.05, pair-wise comparison) or SPA/IFN (P=0.05, pair-wise comparison). No significant differences in body weight, temperature, hematocrit, red blood cell counts, mean corpuscular hemoglobin concentration, reticulocyte counts, white blood cell or neutrophil numbers, lymphocyte concentrations, bone-marrow cytopathology, FeLV status, survival time, activity, or appetite scores were observed. No significant differences in the owners' subjective assessment of their cat's health following treatment with SAL/IFN, SPA/IFN, or SAL/SAL were seen. Therapy with SPA as a single agent results in the owners' subjective impression of improved health of their FeLV-infected cats. 相似文献
113.
114.
Wakim LM Waithman J van Rooijen N Heath WR Carbone FR 《Science (New York, N.Y.)》2008,319(5860):198-202
Secondary lymphoid organs are dominant sites of T cell activation, although many T cells are subsequently retained within peripheral tissues. Currently, these nonlymphoid compartments are viewed as sites only of effector T cell function, without the involvement of renewed induction of immunity via the interactions with professional antigen-presenting cells. We describe a method of reactivation of herpes simplex virus to examine the stimulation of tissue-resident T cells during secondary challenge. The results revealed that memory CD8+ T cell responses can be initiated within peripheral tissues through a tripartite interaction that includes CD4+ T cells and recruited dendritic cells. These findings lend evidence for the existence of a sophisticated T cell response mechanism in extra-lymphoid tissues that can act to control localized infection. 相似文献
115.
Park HJ Park JS Hayek MG Reinhart GA Chew BP 《Veterinary immunology and immunopathology》2011,143(3-4):301-306
Type-I interferons (IFNs) are cytokines that have non-specific antiviral activity, participating mostly in innate defense mechanisms. Their administration has been proposed to treat several viral and immunomediated diseases as an immunomodulatory therapy. Due to its availability, recombinant human interferon-alpha (rHuIFN-α) has been studied in relation to feline retrovirosis, both in vitro and in vivo. However, IFNs are species-specific and antibodies have been shown to develop in response to the high rHuIFN-α doses necessary for an effective therapy. A recombinant feline IFN has been developed, which has been characterized as interferon-omega (rFeIFN-ω), designed to overcome these problems. Nonetheless, very few studies have been undertaken to evaluate its efficacy in cats naturally infected with FIV or FeLV. In an initial study, we here demonstrated that rFeIFN-ω can dramatically improve the clinical condition of infected cats, and induce improvement of hematologic parameters. Minor changes or no change was observed for hypergammaglobulinemia, CD4/CD8 ratio, proviral load, viremia and RT activity, suggesting that the overall effect of IFN was on innate immunity. More studies are needed in order to better understand its in vivo mechanisms. 相似文献
116.
Bechtluft P van Leeuwen RG Tyreman M Tomkiewicz D Nouwen N Tepper HL Driessen AJ Tans SJ 《Science (New York, N.Y.)》2007,318(5855):1458-1461
How chaperone interactions affect protein folding pathways is a central problem in biology. With the use of optical tweezers and all-atom molecular dynamics simulations, we studied the effect of chaperone SecB on the folding and unfolding pathways of maltose binding protein (MBP) at the single-molecule level. In the absence of SecB, we find that the MBP polypeptide first collapses into a molten globulelike compacted state and then folds into a stable core structure onto which several alpha helices are finally wrapped. Interactions with SecB completely prevent stable tertiary contacts in the core structure but have no detectable effect on the folding of the external alpha helices. It appears that SecB only binds to the extended or molten globulelike structure and retains MBP in this latter state. Thus during MBP translocation, no energy is required to disrupt stable tertiary interactions. 相似文献
117.
118.
119.
The relationships between Penaeus monodon production and three crop characteristics in 107 ponds on nine shrimp farms in East Java were studied. Overall differences of production (Mt ha-1 year-1), survival rate (%), stocking density (number m-2) and mean weight at harvest (g) between regions and between farms were significant, while the difference of production between farms was also significant within regions. Overall analysis revealed that production was positively correlated with survival rate, stocking density and mean weight at harvest, while there was a negative correlation between stocking density and survival rate. Results imply that more attention should be paid to improving shrimp survival. The higher on-farm production in South East Java is linked to higher survival rates and higher post-larvae stocking densities than in North West and North East Java, while similar individual weights at harvest to North West East Java are reached. In this context, the relationship between coastal estuarine water quality and yield needs further investigation. Other parameters which need further investigation are farm management, post-larvae origin and duration of the culture period. 相似文献
120.
1. Two experiments were carried out in a Scottish river to test the effects of electrofishing on endangered freshwater pearl mussels (Margaritifera margaritifera). 2. In the first experiment, two areas of mussel bed were marked out as treatment and control sites and the former was electrofished using standard equipment. The mussels were examined 10 min, 24 h and 35 days after treatment and their shell valve closure responses, burrowing capabilities and gravidities were recorded as signs of normal ‘functioning’. 3. In the second experiment, individual mussels were marked as treatments and controls and the former were electrofished. These mussels were examined as before. 4. No mortalities occurred and no significant differences in ‘functioning’ between treatments and controls were observed in either experiment. 5. Since no measurable treatment effect could be demonstrated, it appears that electrofishing did not adversely affect the short‐term survival of M. margaritifera. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献