Allozyme variation of littleneck clam Ruditapes philippinarum and genetic mixture analysis of foreign clams in Ariake Sea and Shiranui Sea off Kyushu Island, Japan     

Kelly Vargas  Yoshiho Asakura  Minoru Ikeda  Nobuhiko Taniguchi  Yasuhiro Obata  Katsuyuki Hamasaki  Kotaro Tsuchiya  Shuichi Kitada 《Fisheries Science》2008,74(3):533-543

ABSTRACT:   Allozyme variation of the littleneck clam Ruditapes philippinarum was evaluated in four samples from Nameishi and Matsuo in the Ariake Sea, Ryugatake and Ushibuka in the Shiranui Sea off Kyushu Island, Japan, and in one sample from Jinzhou, China, in the Bohai Sea. A Ruditapes bruguieri sample imported from the Korean Bay off Nampo, North Korea was also studied. Among the R. philippinarum samples, heterozygosity varied from 0.265 to 0.301 and F _IS estimates indicated significant homozygosity excess in 15 of 40 loci analyzed. Deviations from Hardy–Weinberg equilibrium were significant in all samples ( P  < 0.05). Pairwise F _ST estimates indicate that genetic differences between the Chinese and Japanese samples were very low, but significantly different from zero. Mixture proportions with 95% confidence intervals of Chinese R. philippinarum in Nameishi and Matsuo were estimated at 0.4098 [0.2512, 0.5705] and 0.4899 [0.3262, 0.6540], respectively. However, genetic invasion of stocked Chinese R. philippinarum into wild populations in the Ariake Sea remains uncertain due to the low precision of the estimates caused by the high similarity of allele frequencies between Jinzhou and the Ariake Sea.  相似文献   

Accumulation of paralytic shellfish poisoning toxin by the swimming crab <Emphasis Type="Italic">Charybdis japonica</Emphasis> in Kure Bay,Hiroshima Prefecture     

Hiroshi Oikawa  Yukihiko Matsuyama  Masataka Satomi  Yutaka Yano 《Fisheries Science》2008,74(5):1180-1186

The accumulation of paralytic shellfish poisoning (PSP) toxin in the crab Charybdis japonica was investigated in Kure Bay, when a bivalve Mytilus galloprovincialis, in the same water, showed toxicity caused by PSP toxin. In 2005, 17 specimens of the crab had PSP toxin over 4 MU/g in the hepatopancreas, and the highest toxicity was at 37.4 MU/g. Since the regulation limit of PSP for crab hepatopancreas was set in 2004, this is the first observation of regulatory level of PSP toxin in C. japonica. Unlike in the hepatopancreas of the crab, the hazardous level of the toxin was not detected in the muscle of the cephalothorax and the appendage. The toxin accumulation in the crab was also investigated by feeding toxic mussels to the crab. The crab retained the toxin mainly in the hepatopancreas, and the ratio of retention in the crab was from 12.9 to 24.6%. The toxin profiles, shown in the feeding experiments, suggest that the transformation of PSP toxin occurs in the crab because dcGTX2 and dcGTX3 was detected in all crab specimens despite the lack of these analogs in the mussels used as feed.  相似文献   

Clonal structure in Ichthyobacterium seriolicida,the causative agent of bacterial haemolytic jaundice in yellowtail,Seriola quinqueradiata,inferred from molecular epidemiological analysis          下载免费PDF全文

T Matsuyama  Y Fukuda  T Sakai  N Tanimoto  M Nakanishi  Y Nakamura  T Takano  C Nakayasu 《Journal of fish diseases》2017,40(8):1065-1075

Bacterial haemolytic jaundice caused by Ichthyobacterium seriolicida has been responsible for mortality in farmed yellowtail, Seriola quinqueradiata, in western Japan since the 1980s. In this study, polymorphic analysis of I. seriolicida was performed using three molecular methods: amplified fragment length polymorphism (AFLP) analysis, multilocus sequence typing (MLST) and multiple‐locus variable‐number tandem repeat analysis (MLVA). Twenty‐eight isolates were analysed using AFLP, while 31 isolates were examined by MLST and MLVA. No polymorphisms were identified by AFLP analysis using EcoRI and MseI, or by MLST of internal fragments of eight housekeeping genes. However, MLVA revealed variation in repeat numbers of three elements, allowing separation of the isolates into 16 sequence types. The unweighted pair group method using arithmetic averages cluster analysis of the MLVA data identified four major clusters, and all isolates belonged to clonal complexes. It is likely that I. seriolicida populations share a common ancestor, which may be a recently introduced strain.  相似文献   

Age validation,growth and annual reproductive cycle of chub mackerel <Emphasis Type="Italic">Scomber japonicus</Emphasis> off the waters of northern Kyushu and in the East China Sea     

Tetsuro Shiraishi  Kumiko Okamoto  Michio Yoneda  Takeshi Sakai  Seiji Ohshimo  Shizumasa Onoe  Akihiko Yamaguchi  Michiya Matsuyama 《Fisheries Science》2008,74(5):947-954

The age and growth of chub mackerel Scomber japonicus collected from the East China Sea and the northern waters off Kyushu between June 2000 and June 2001 were determined by observing the otolith surface after dipping it in xylene. The translucent and opaque zones on the otolith surface were identified, and the number of translucent zones was counted. Monthly changes in the frequency of fish with translucent zones on the otolith margin, and in the marginal increments, indicated that the translucent zones were formed between April and June. The seasonal pattern of annulus formation on the otolith became clear by observing the otoliths of fish with known ages, and the otolith formation in wild fish was consistent with that of fish with known ages. The mean gonadosomatic index of male and female fish was high from March to May, and spawning females were observed from mid-March to mid-May. The estimated ages were 1–5 years for males and 1–6 years for females. The von Bertalanffy growth parameters did not significantly differ between male and female. The model was obtained as FL _t=406×{1−exp[−0.372×(t+1.68)]  相似文献   

Nephrocalcinosis formation by soy isoflavones in male rats     

Satoh T  Yamakoshi J  Saito M  Izumi T  Matsuyama A  Kikuchi M  Yamaguchi T 《Journal of agricultural and food chemistry》2006,54(15):5659-5663

Isoflavones (IFs), found in the form of both aglycones and glucosides in soybean foods, induce weak estrogenic activities. Although IFs have a number of health benefits, it was previously reported that IFs cause nephrocalcinosis (NC) in the kidney of male Fischer 344 (F344) rats. The present study aims to elucidate the safety of IFs by focusing on IF-induced NC formation in rats. Fermented soybean extract (FSE) containing 420 mg/g isoflavone aglycones was orally administered to male F344 and Sprague-Dawley (SD) rats for 28 days. FSE induced NC formation in the kidney of F344 rats, but not in SD rats. However, absorption of IFs did not differ between F344 and SD rats. NC formation and its severity of FSE were histologically compared with those of soybean extract (SE) containing 518 mg/g isoflavone glucosides in F344 rats. There were no differences in the number of NC formations and the extent of calcium deposit between FSE and SE groups. To examine the dose effect of FSE on NC formation, doses of 20, 140, or 1000 mg/kg FSE were administered to F344 rats for 90 days. NC formation was observed in the 140 and 1000 mg/kg groups. These results indicated that a high dose of oral administration of IFs induced NC formation depending on the strain of rat.  相似文献   

A simulation study for a linear measurement error model when error variances vary between measurements     

Yasutaka?ChibaEmail author  Yutaka?Matsuyama  Tosiya?Sato  Isao?Yoshimura 《Journal of Agricultural, Biological & Environmental Statistics》2005,10(1):118-130

When predicting scores in the Draize eye irritation test based on measurements of in vitro alternative tests, we are often faced with estimating parameters in a linear measurement error model with heterogeneous error variances. This article proposes a new statistical method for parameter estimation to address this issue. The proposed method is an extension of an earlier proposal that applied a linear measurement error model with homogeneous error variances, to cases with heterogeneous error variances. A simulation study to examine the performance of the proposed method was conducted in a framework that was adaptable to the data, which was obtained in a validation study of alternative methods to animal experiments conducted in Japan. The proposed method reduced the biases of estimates in comparison with an ordinary regression analysis method and three other methods under the assumption of homogeneous error variances. Although the proposed method did not fit the real data well, the resulting prediction formula was far better than those obtained by other methods.  相似文献   

Mammary lesions associated with bovine herpesvirus type 4 in a cow with clinical mastitis     

Miyano H  Haritani M  Sentsui H  Tsuboi T  Tanimura N  Kimura KM  Kobayashi M  Obara N  Akimoto Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):457-460

Intranuclear eosinophilic inclusion bodies were seen in the lactiferous duct and sinus epithelium of mammary tissues collected from a cow with clinical mastitis. Transmission electron microscopy revealed herpesvirus particles in these cells. Immunolabeling against anti bovine herpesvirus type 4 (BHV-4) rabbit serum was detected in nuclei that had intranuclear inclusion bodies. In addition, BHV-4 was isolated from the mammary tissue. The viral DNA was detected by nested PCR from the same tissue. This is the first report to describe mammary lesions in association with BHV-4.  相似文献   

Unpredictable Chronic Stress-Induced Reproductive Suppression Associated with the Decrease of Kisspeptin Immunoreactivity in Male Mice     

Tetsushi HIRANO  Yoshihiro KOBAYASHI  Takuya OMOTEHARA  Atsutoshi TATSUMI  Rie HASHIMOTO  Yuria UMEMURA  Daichi NAGAHARA  Youhei MANTANI  Toshifumi YOKOYAMA  Hiroshi KITAGAWA  Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(9):1201-1208

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REV-ERBα Inhibits the PTGS2 Expression in Bovine Uterus Endometrium Stromal and Epithelial Cells Exposed to Ovarian Steroids     

Keishiro ISAYAMA  Huatao CHEN  Nobuhiko YAMAUCHI  Masa-aki HATTORI 《The Journal of reproduction and development》2014,60(5):362-370

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Effects of in utero and lactational exposure to the no-observed-adverse-effect level (NOAEL) dose of the neonicotinoid clothianidin on the reproductive organs of female mice     

Sayaka KITAUCHI  Mizuki MAEDA  Tetsushi HIRANO  Yoshinori IKENAKA  Misaki NISHI  Asuka SHODA  Midori MURATA  Youhei MANTANI  Toshifumi YOKOYAMA  Yoshiaki TABUCHI  Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):746

Recently, developmental exposure to clothianidin (CLO) has been shown to cause reproductive toxicity in male mice, but the effects in female mice remain to be clarified. Pregnant C57BL/6N mice were given a no-observed-adverse-effect-level (NOAEL) dose of CLO until weaning. We then examined ovaries of 3- or 10-week-old female offspring. In the CLO-administered group, morphological changes, a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 (GPx4), and activation of genes in the steroid hormone biosynthesis pathway were observed in 3-week-old mice, and decreases of GPx4 immunoreactivity, 17OH-progesterone and corticosterone levels were observed in 10-week-old mice, along with high rates of infanticide and severe neglect, providing new evidence that developmental exposure to CLO affects juvenile and adult mice differently.  相似文献