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291.
292.
Out of 851 soybean accessions from Vietnam, China and Japan analyzed for 7S β-subunit variants, a new β-reduced subunit line with normal growth was collected from the Mekong Delta, Vietnam. Protein of the `β-reduced' line is composed of β-reduced and extremely low-β types. (α-null + β-reduced) type and β-null (or extremely low-β) type were screened in the progeny seeds of the Japanese mutant `(α + β) null' line. Therefore, recombination between α- and β-subunits was identified. By comparing the nucleotide sequence of the partial β-subunit gene of Enrei (standard), Mo-shi-dou Gong 503 (α + β low), β-533 seed (β-reduced), β-57 seed (extremely low-β), and (α + β)-null16((α + β) null) seed, we found that the base `T' at 166 bp, in Enrei changed to `G' in Mo-shi-dou Gong 503, β-533 and β-57. Using the (α + β)-null16 individual as template, a distinct 305 bp β-subunit gene fragment was identified, instead of a 285 bp fragment.  相似文献   
293.
The distribution of heterotrophic bacteria on organic debris and roots of rice plants in a paddy field were studied. The heterotrophic bacteria consisted of two main groups: those which grew on full-strength nutrient broth (NB) and those which did not grow on NB but on a 100-fold dilution of NB (DNB). The latter group was called ‘DNB organisms’ and were considered to be oligotrophic. In both manured and unmanured soils, DNB organisms were predominant in the bacterial communities on organic debris and the rice roots throughout most of the entire period of rice cultivation, although a transient decrease in the proportion of DNB organisms was observed immediately after an application of manure. Morphological and physiological characteristics of DNB isolates from organic debris and rice roots were studied: five types of cell shape were observed, (1) regular rods, (2) filament-forming rods, (3) irregular rods, (4) prosthecate organisms and (5) large oval cells. Regular rods (42% of the total DNB isolates) and irregular rods (46%) were abundant. The ecological roles of DNB organisms in paddy soil are discussed in relation to their physiological characteristics.  相似文献   
294.
The mobility and degradation of imazosulfuron, labeled with carbon-14 at the imidazole ([imi-(14)C]imazo) or pyrimidine ring ([pyr-(14)C]imazo), in lysimeters with 1 m(2) surface and 110 cm depth were investigated for three years. One lysimeter was treated with [imi-(14)C]imazo in two successive years at the rate of 50 g of active ingredient (ai)/ha each. The other two lysimeters were treated once with [pyr-(14)C]imazo and a mixture (1:1, w/w) of the two labeled imazosulfurons, respectively (50 g of ai/ha). In the first and second years of monitoring, the yearly mean concentration of (14)C in the leachate water was <0.10 microg/L in each lysimeter. Although in the third year the concentration of (14)C in the leachate water was 0.17 microg/L for the lysimeter treated twice with [imi-(14)C]imazo, the concentration of imazosulfuron and its degradation products in the leachate water ranged from 0.01 to 0.06 microg/L. At the study termination, the main portion of (14)C recovered was found in the upper 30 cm soil layer in each lysimeter, and no (14)C was detected below a depth of 50 cm. These findings indicated that imazosulfuron and its degradation products in soils translocated into groundwater only slightly.  相似文献   
295.
296.
Recent discovery of a phase transition from perovskite to post-perovskite suggests that the physical properties of Earth's lowermost mantle, called the D' layer, may be different from those of the overlying mantle. We report that the electrical conductivity of (Mg0.9Fe0.1)SiO3 post-perovskite is >10(2) siemens per meter and does not vary greatly with temperature at the conditions of the D' layer. A post-perovskite layer above the core-mantle boundary would, by electromagnetic coupling, enhance the exchange of angular momentum between the fluid core and the solid mantle, which can explain the observed changes in the length of a day on decadal time scales. Heterogeneity in the conductivity of the lowermost mantle is likely to depend on changes in chemistry of the boundary region, not fluctuations in temperature.  相似文献   
297.
The extract of the sea hare, Aplysia kurodai, showed neurotrophic activity toward rat pheochromocytoma (PC-12) cells. Bioassay-guided purification afforded two active compounds, which were subsequently identified to be parguerol and isoparguerol by spectroscopic analysis. It was found that both parguerol and isoparguerol induced neurite outgrowth in PC-12 cells at concentrations of 25 and 50 μM, respectively.  相似文献   
298.
We investigated spatial structures of N2O, CO2, and CH4 fluxes during a relatively dry season in an Acacia mangium plantation stand in Sumatra, Indonesia. The fluxes and soil properties were measured at 1-m intervals in a 1 × 30-m plot (62 grid points) and at 10-m intervals in a 40 × 100-m plot (55 grid points) at different topographical positions of the upper plateau, slope, and valley bottom in the plantation. Spatial structures of each gas flux and soil property were identified using geostatistical analysis. The means (±SD) of N2O, CO2, and CH4 fluxes in the 10-m grids were 0.54 (±0.33) mg N m−2 d−1, 2.81 (±0.71) g C m−2 d−1, and −0.84 (±0.33) mg C m−2 d−1, respectively. This suggests that A. mangium soils function as a larger source of N2O than natural forest soils in the adjacent province on Sumatra during the relatively dry season, while CO2 and CH4 emissions from the A. mangium soils were less than or consistent with those in the natural forest soils. Multiple spatial dependence of N2O fluxes within 3.2 m (1-m grids) and 35.0 m (10-m grids), and CO2 fluxes within 1.8 m (1-m grids) and over 65 m (10-m grids) was detected. From the relationship among N2O and CO2 gas fluxes, soil properties, and topographic elements, we suggest that the multiple spatial structures of N2O and CO2 fluxes are mainly associated with soil resources such as readily mineralizable carbon and nitrogen in a relatively dry season. The soil resource distributions were probably controlled by the meso- and microtopography. Meanwhile, CH4 fluxes were spatially independent in the A. mangium soils, and the water-filled pore space appeared to mainly control the spatial distribution of these fluxes.  相似文献   
299.
This study examines the relationship between spike morphology and natural habitat for 84 accessions of four Aegilops species, belongs to section Sitopsis, Ae. bicornis, Ae. longissima, Ae. searsii, and Ae. sharonensis in genus Aegilops, section Sitopsis, wild relatives of Triticum aestivum L. These species are considered valuable genetic resources for future cultivation and breeding of domesticated wheat. The goals of the study were to: (1) document variation in spike morphology among these four species; (2) examine the relationship between spike morphology and native habitat; (3) document geographical distribution of distinct spike morphology; and (4) examine the relationship between spike morphology and heading time and value for these four species. The results reveal significant differences in spike morphology among species of section Sitopsis. The most noteworthy variation involved the absence/presence of lateral awn, such that species with lateral awn were restricted in coastal, though species without lateral awn were mainly distributed in inland. This suggests that local climate may be a determinant of variation in lateral awn, and that this trait may be subject to convergent evolution. Differences in heading time in sympatric area were also observed. The differences may enhance species divergence and could represent a lead speciation event. The results of this study will facilitate identification of populations or accessions of wild wheat with favorable traits and/or novel adaptive genes.  相似文献   
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