Reduced immunogenicity of beta-lactoglobulin by conjugation with carboxymethyl dextran differing in molecular weight   总被引：2，自引：0，他引：2  

Kobayashi K  Hirano A  Ohta A  Yoshida T  Takahashi K  Hattori M 《Journal of agricultural and food chemistry》2001,49(2):823-831

To reduce the immunogenicity of beta-lactoglobulin (beta-LG), two beta-LG-carboxymethyl dextran (CMD) conjugates (Conj. 40 and Conj. 162) were prepared by using water-soluble carbodiimide (EDC). The molar ratios of beta-LG to CMD in Conj. 40 and Conj. 162 were 8:1 and 7:1, respectively. Each conjugate maintained approximately 50% of the retinol binding activity of beta-LG. Structural analyses by intrinsic fluorescence, CD spectra, and ELISA with monoclonal antibodies indicated that the surface of beta-LG in each conjugate was covered by CMD without great disruption of native conformation. By conjugation with CMD, the antibody response to beta-LG was reduced in BALB/c, C3H/He, and C57BL/6 mice, which was eminent in Conj. 162. The results of B cell epitope scanning using overlapping synthesized peptides showed that the linear epitope profiles of the conjugates were similar to those of beta-LG, whereas the antibody response to each epitope was reduced, which was eminent in Conj. 162. It was concluded that conjugation with CMD of higher molecular weight is effective in reducing the immunogenicity of beta-LG and that masking of epitopes by CMD is responsible for the reduced immunogenicity.  相似文献   

Helminth infections of dogs in Shiga, Japan with reference to occult infection of Dirofilaria immitis.     

Y Tada  T Ohta  S Soohara  Y Suzuki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(2):359-360

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Simple isolation of canine C-reactive protein (CRP) by phosphorylcholine (PC) affinity chromatography.     

H Fujise  H Takanami  M Yamamoto  I Ohta  S Yamamoto  T Fukase  M Naiki  S Akihama  E Ogawa  R Takahashi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(1):165-167

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Full-term development of offspring using round spermatids produced ectopically from fetal male germ cells   总被引：8，自引：0，他引：8  

Ohta H  Wakayama T 《The Journal of reproduction and development》2004,50(4):429-437

The continuous production of mammalian sperm is maintained by the proliferation and differentiation of spermatogonial stem cells, which originate from primordial germ cells in the early embryo. Previously, we reported that the transplantation of fetal male gonadal tissue into the recipient testis was effective obtaining functional sperm. This transplantation technique is a promising new approach for the preservation of testicular function in a mutant animal with embryonic lethality. In the present study, we examined whether spermatogenesis from fetal male germ cells is induced under ectopic conditions in male and female recipients. Nine to 10 weeks after the transplantation of male gonads prepared from embryos at 12.5 or 16.5 days post gestation, male germ cell differentiation occurred under the skin of male and female recipient nude mice. Histological analyses revealed that grafted gonads contained haploid germ cells such as round or elongated spermatids. Furthermore, we succeeded in obtaining normal progeny by injecting the ectopically produced round spermatids into the cytoplasm of oocytes, even when the male germ cells had been generated in female recipients. These results indicate that the transplantation of fetal male gonads under the skin of recipient mice is a useful technique for obtaining functional male gametes.  相似文献   

Sugar expressions on the vaginal epithelium in pregnant mice     

Yasunaga Y  Takeuchi T  Shimokawa T  Nabeta M  Matsuu A  Asano A  Ohta Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):805-808

Sugar expressions were examined on the epithelium of both the middle portion of the vagina and the vaginal portion of the cervical canal (CC) in pregnant mice to understand the pathogenesis of bacterial infection in the female reproductive organ by using a panel of lectins. As a result, N-acetylglucosamine was positive before pregnant day (P) 7 but negative after P10 and at diestrus on both the vagina and the CC. In addition, some differences in sugar expressions were seen between them. These results suggest that sugar expressions on the mucosal surface would change not only site-specifically but also time-dependently, and these sugar differences indicate the possibility of the alteration of the settled bacterial species on the vaginal mucosa in pregnancy.  相似文献   

Parathyroid hormone (1-34) improves bone mineral density and glucose metabolism in Spontaneously Diabetic Torii-Lepr(fa) rats     

Kimura S  Sasase T  Ohta T  Sato E  Matsushita M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):103-105

The Spontaneously Diabetic Torii-Lepr(fa) (SDT-fa/fa) rat, a model of obese type 2 diabetes, shows obesity, hyperglycaemia and low bone mineral density (BMD). The objective of this study is to evaluate the effects of parathyroid hormone (1-34) [PTH(1-34)] on BMD and glucose metabolism in the SDT-fa/fa rat. SDT-fa/fa rats showed obesity with hyperglycaemia and decreased serum osteocalcin levels and the tibial BMD. A 4-week treatment of PTH(1-34) (20 μg/kg/day) increased the serum osteocalcin levels and the tibial BMD, and decreased the serum glucose levels without changing the serum insulin levels. These findings indicate that PTH(1-34) improved not only BMD but also glucose metabolism in SDT-fa/fa rats. This study suggests that PTH(1-34) is a novel agent for the treatment of diabetic osteoporosis.  相似文献   

Bilateral segmental aplasia with unilateral uterine horn torsion in a pomeranian bitch     

K Nakamura  M Yamasaki  T Osaki  H Ohta  N Sasaki  K Aoshima  T Kimura  M Takiguchi 《Journal of the American Animal Hospital Association》2012,48(5):327-330

Bilateral segmental aplasia of the uterine horns with unilateral pyometra and uterine horn torsion were diagnosed in a Pomeranian bitch that presented with chronic abdominal distension and an acute onset of anorexia and lethargy. Because radiographic and ultrasonographic findings revealed the presence of markedly enlarged bilateral uterine horns filled with fluid in the caudal abdomen, a tentative diagnosis of either pyometra or hydrometra with uterine horn torsion was made. Exploratory laparotomy showed bilateral, segmentally distended uterine horns with unilateral uterine horn torsion. Ovariohysterectomy was performed, and bilateral segmental aplasia of the uterine horns with the development of unilateral uterine horn torsion was diagnosed histopathologically. To the authors' knowledge, this is the first report of uterine horn torsion in conjunction with segmental aplasia of the uterine horn in a bitch.  相似文献   

Seasonal variations in the incidence of pine wilt and infestation by its vector, Monochamus alternatus, near the northern limit of the disease in Japan     

Kazumasa Ohta  Kazuhiko Hoshizaki  Katsunori Nakamura  Akihiko Nagaki  Yoichi Ozawa  Aoi Nikkeshi  Akifumi Makita  Kazumi Kobayashi  Osamu Nakakita 《Journal of Forest Research》2012,17(4):360-368

During the last few decades, pine wilt disease has spread to cool-climate regions in Japan and, more recently, the potential risk of it spreading into the European midwest has also become a concern. In a coastal pine stand (84.7?ha) in Akita, near the northern limit of pine wilt disease in Japan, we investigated seasonal variations in the incidence of damage caused by the disease to trees and oviposition by the disease’s insect vector, Monochamus alternatus, during a two-year period. Foliage discoloration occurred throughout each year, and its seasonal variation showed a bimodal pattern in Pinus thunbergii (a higher peak in May–June and a smaller peak in October) and a clear peak in June in P. densiflora, which differed from the patterns in seasonal variation seen for warm-climate regions. Oviposition scars by M. alternatus were found in 40–45% of the trees damaged each year. The percentage of trees that had oviposition scars was higher in P. thunbergii than in P. densiflora. This appeared to reflect the difference in seasonal discoloration pattern between the two species. Analysis of the oviposition risk showed that trees that exhibited discoloration starting between July and October had a significantly higher risk or significantly higher oviposition scar densities, particularly for those that became discolored between August and September (2.5–14.6-fold higher risk than during other months). Oviposition scar densities per damaged tree were similar within the period of higher oviposition risk. Considering both oviposition risks and scar densities, we concluded that trees with discoloration that become apparent between July and October are important targets for preventing the spread of pine wilt disease in Akita.  相似文献   

Expression of CD4 T cell cytokine genes in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds     

Hiroshi Ohta  Kanae Takada  Shidow Torisu  Masashi Yuki  Yu Tamura  Nozomu Yokoyama  Tatsuyuki Osuga  Sue Yee Lim  Masahiro Murakami  Noboru Sasaki  Kensuke Nakamura  Masahiro Yamasaki  Mitsuyoshi Takiguchi 《Veterinary immunology and immunopathology》2013

Inflammatory colorectal polyps (ICRPs) in miniature dachshunds are recently recognized as a major cause of large bowel diarrhea in this dog breed in Japan. ICRPs are characterized by the formation of multiple small polyps and a space-occupying large polyp in the colorectal area, and are thought to be a novel form of inflammatory bowel disease (IBD). In humans, specific cytokine patterns attributed to T helper (Th)1, Th17 and regulatory T cells have important roles in the pathogenesis of IBD. Thus, the aim of the present study was to assess the gene expression of cytokines of T cell subsets in the colorectal mucosa from dogs with ICRPs. Colorectal mucosal specimens from 10 dogs with ICRPs and 14 control dogs were used in this study. Interferon (IFN)-γ, interleukin (IL)-4, IL-17A and IL-10 mRNA expression was assessed using quantitative real-time PCR. IL-17A mRNA expression was significantly increased in large polyps compared to small polyps and controls. IFN-γ and IL-10 mRNA expression in large polyps were significantly higher than in controls. There was no significant difference in IL-4 mRNA expression among the three groups. IL-17A is thought to play important roles in the pathogenesis of ICRPs. IL-10 up-regulation could oppose the proinflammatory function of IL-17A.  相似文献   

Changes in osmotic pressure that trigger the initiation of spermmotility in the river sculpin Cottus hangiongensis     

H. Ohta  Y. Shinriki 《Fish physiology and biochemistry》1998,18(1):29-35

The effects of changes in osmotic pressure on the initiation of spermmotility in the river sculpin were studied. When the fresh milt was diluteddirectly with solutions of mannitol at various concentrations, 22% ofthe spermatozoa became motile in isotonic (300 mM) solution, 63%became motile in a solution of 200 mM, and more than 90% becamemotile in a solution of 100 mM and in 20 mM HEPES-NaOH. When the spermatozoawere transferred to various osmotic conditions after the termination ofswimming in the isotonic solution, 27.9% of spermatozoa became motilein the solution of 200 mM, while more than 80% of spermatozoa becamemotile in the solution of 100 mM and in the buffer solution. Aftertermination of swimming in the solutions of 300 mM and 200 mM, more than80% of spermatozoa became motile again when they were transferred toa solution of 100 mM and to the buffer solution. After the termination ofswimming in the solution of 100 mM, most of spermatozoa were immotile evenwhen they were transferred to the buffer solution. These results suggest theexistence of multiple osmotic switches in each sperm cell that initiate themotility; one reacts in response to the osmotic conditions above 200 mOsmkg^-1, with the threshold varying for each cll, while anotherswitch, which is common to all the cells, reacts in response to osmoticpressure below 200 mOsm kg^-1. The existence of the latterswitch was confirmed by the motility in the solution of 100 mM and in thebuffer solution in this study.  相似文献