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131.
Geraniol 10-hydroxylase (G10H), a cytochrome P450 monooxygenase, has been reported to be involved in the biosynthesis of terpenoid indole alkaloids. The gene for Catharanthus roseus G10H (CrG10H) was cloned and heterologously expressed in baculovirus-infected insect cells. A number of substrates were subjected to assay the enzyme activity of CrG10H. As reported in a previous study, CrG10H hydroxylated the monoterpenoid geraniol at the C-10 position to generate 10-hydroxygeraniol. Interestingly, CrG10H also catalyzed 3'-hydroxylation of naringenin to produce eriodictyol. Coexpression of an Arabidopsis NADPH P450 reductase substantially increased the ability of CrG10H to hydroxylate naringenin. The catalytic activity of CrG10H was approximately 10 times more efficient with geraniol than with naringenin, judged by the k(cat)/K(m) values. Thus, G10H also plays an important role in the biosynthetic pathway of flavonoids, in addition to its previously described role in the metabolism of terpenoids. 相似文献
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Gainey PL 《Science (New York, N.Y.)》1918,48(1232):139-140
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Detailed examinations were made of the central permanent incisors and periodontia of 30 or 40 ewes on each of one control farm and three periodontal disease (P.D.) farms. The study confirmed that incisor crown lengthening, protrusion and loosening were significant features of P.D. Periodontitis (assessed by increased sulcus depth and bleeding after probing) about the lingual sulcus was a significant feature of P.D. Labial gingivitis and labial periodontitis were not significant features. A relatively long lower jaw did not appear to be a feature of P.D. 87. 相似文献
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Jenkins PL Stack SG Earle-Richardson GB Scofield SM May JJ 《Journal of agricultural safety and health》2007,13(1):57-64
Dairy farmers may be exposed to high levels of noise and dust. Protections against these hazards exist, but many farmers do not use them. An intervention consisting of hearing and respiratory screenings combined with personalized education was implemented. This study evaluates the impact of this intervention on farmers' self-reported use of personal protective equipment (PPE) and implementation of noise and dust abatement. Participants were screened as to noise (n=209) or dust (n=392) hazards and use of PPE. Following this, they were counseled on PPE use, and identification and reduction of noise or dust hazards. Counselors sought a pledge from the farmers to eliminate hazards and increase PPE use. Farmers were subsequently surveyed and asked whether they had implemented the changes. At baseline, 70% (146/209) of farmers exposed to high levels of noise reported poor use ("sometimes", "rarely", or "never") of hearing protection. Results indicated that two months after intervention, 25.2% (28/111) of these subjects had successfully improved their PPE use. At baseline, 79% (311/392) offarmers reported poor use of respiratory protection, with 27.3% (41/150) showing improvement in PPE use within the same time. Strategies to reduce noise hazards were identified by 92.8% (194/209) of hearing screening attendees; 13.2% (18/136) successfully reduced or removed exposure. These values for dust screening attendees were 98.2% (385/392) and 30.7% (54/176), respectively. Use of this intervention appears to be an effective method for increasing PPE use on the farm. However, it is not effective for reducing noise hazards. 相似文献
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Lefebvre HP Jeunesse E Laroute V Toutain PL 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(3):499-507
Ramipril, an angiotensin-converting enzyme (ACE) inhibitor for use in dogs, is converted in vivo to its active form, ramiprilat, which is eliminated in the bile and urine in the dog. The objective of this study was to assess the effect of renal impairment on the pharmacokinetics (PKs) and pharmacodynamics (PDs) of ramipril and ramiprilat. Ten adult Beagle dogs were used. PK/PD studies were performed before and after the induction of subclinical renal impairment. Ramiprilat was given at 0.25 mg/kg by a single IV bolus. After a 2-week washout period, ramipril was administered PO at 0.25 mg/kg once daily for 8 days. Ramipril and ramiprilat PKs were studied by using a physiologically based model. The relationship between free plasma ramiprilat concentration and ACE activity was described by using the fractional Hill model. Glomerular filtration rate was decreased by 58%. No biologically relevant changes in usual plasma variables were observed between the 1st and the 8th day of oral treatment with ramipril under either condition. After an IV bolus of ramiprilat, the only changes in renal-impaired dogs were a 14 and 49% decrease in clearance of the free fraction of ramiprilat (P < .01) and free plasma concentration required to produce 50% of the maximal effect (P < .05), respectively. After repeated PO administration of ramipril, there were no alterations in any of the PK and PD parameters in healthy or renal-impaired dogs. No adjustment of the recommended PO dosage of ramipril is needed in dogs with moderate renal impairment. 相似文献
140.
May JJ Sorensen JA Burdick PA Earle-Richardson GB Jenkins PL 《Journal of agricultural safety and health》2006,12(3):199-213
Tractor overturns contribute significantly to fatalities in New York State agriculture. On-site inspections a decade ago indicated that approximately 60% of tractors were without effective rollover protection. Our objectives were: to describe the current prevalence and distribution of rollover protective structures (ROPS) on New York farm tractors, to identify characteristics associated with the absence of ROPS, to explore segmenting the New York farm community on readiness for ROPS retrofitting, and to identify demographic characteristics that might assist in this segmenting. A random selection of 644 livestock, dairy, fruit, cash crop, vegetable, and organic farms were contacted for a telephone survey. Of 562 farms (87%) participating, 102 (18.1%) had all tractors equipped with ROPS and 138 (24.6%) had none. A disproportionate number of livestock, cash crop, and organic operations had no ROPS. Rates of ROPS-equipped tractors correlated directly with farm size and annual hours of tractor operation. Older farmers had a lower proportion of ROPS tractors. The presence of a child operator did not affect the proportion of ROPS tractors. After weighting the sample, the total number of non-ROPS tractors in New York is estimated at more than 80,000. In addition to providing key farm demographics, the survey enabled placement of farmers on a "stage of change" continuum related to readiness for retrofitting. Three-quarters of New York farmers are in the "precontemplation" stage of change relative to ROPS retrofitting, and this varies little by size of operation, age of farmer, or the presence of child tractor operators. Stage of change may relate to hours of tractor operation (p = 0.05) and does relate to commodity (p = 0.003) due primarily to the higher proportion of crop farmers in the earliest stage of change. The goal of retrofitting all New York farm tractors with ROPS appears nearly as daunting as it did a decade ago. 相似文献