Purification and characterization of hepatic triacylglycerol lipase isolated from rainbow trout,Oncorhynchus mykiss     

Jamie S. Harmon  Kim G. Michelsen  Dr. Mark A. Sheridan 《Fish physiology and biochemistry》1991,9(4):361-368

Trialcylglycerol (TG) lipase was isolated and partially purified from rainbow trout liver. Triacylglycerol lipase activity was assayed by measuring¹⁴C-oleic acid release from¹⁴C-triolein.¹⁴C-oleic acid release was linear for up to two hours. Optimal activity occurred at pH 7.0 and 15°C. Most of the lipase activity was recovered in the cytosolic fraction. A 27,000-fold purification was achieved after Sepharose (Bio-gel A 0.5 M, 200–400 mesh) chromatography of a resuspended 20% ammonium sulfate fraction. The molecular weight of the trout hepatic lipase as determined by size-exclusion chromatography and by SDS-polyacrylamide gel electrophoresis was 40–43 kD. Lipase-mediated hydrolysis of TG resulted in the production of diacylglycerols, monoacylglycerols, and fatty acids. Kinetic analysis indicated that V_max=0.016 nmol/h/mg protein and that K_m=0.28 mM triolein. Lipolytic activity was enhanced in the presence of cAMP/ATP-Mg²⁺. These results suggest that the liver of trout possesses a neutral TG lipase that is responsible for mobilizing stored TG and is catalytically activated by phosphorylation.A part of this work was presented at the Annual Meeting of the American Society of Zoologists, December 26–30, 1990, San Antonio, TX.  相似文献   

Physical characteristics and chemical composition of Greater Rhea (Rhea americana) eggs from wild and captive populations.     

J L Navarro  M L López  D M Maestri  D O Labuckas 《British poultry science》2001,42(5):658-662

1. Eggs from wild and captive populations of Greater Rhea (Rhea americana) were studied to determine their physical and chemical characteristics. 2. Significant differences were found among populations in almost all chemical parameters studied, whereas within physical parameters only shell weight (as a proportion of the entire egg) and density showed differences. 3. Eggs from wild populations had the highest protein and linolenic acid and the lowest total lipid contents, while cholesterol levels of these eggs and of those from the largest captive area were the lowest.  相似文献   

Vic Pres speaks at "100th" luncheon     

Dr  Jeff Brady 《Australian veterinary journal》2001,79(1):11-11

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Requirements for less stringent UK pet travel plan     

Dr  Kevin Doyle 《Australian veterinary journal》2001,79(2):78-78

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Maximal lactate concentrations in horses after exercise of different duration and intensity     

A. Lindner  Dr med vet  l;  P. von Wittke  Dr. med vet;  M. Schmald  J. Kusserow  H. Sommer  Dr habil  Dr med vet 《Journal of Equine Veterinary Science》1992,12(1)

Lactate kinetics in whole blood of horses was investigated after exercise of differing velocities and duration. The following categories of exercise were used: A: <11 m/second and >180 seconds (n=35), B: >11 m/second and <180 seconds (n=17) and C: <11 m/second and <180 s (n=10). The mean peak lactate concentration determined in horses in category A was 4.49 ± 2.21 mmol/1, in B, 16.32 ± 4.81 mmoVl and in C, 4.58 ± 1.59 mmol/l. While the maximum lactate concentrations in categories A and C were always found immediately after the exercise, the peaks in category B were measured between the first and tenth minute after exercise. Mean lactate concentrations measured at 2-minute intervals after bouts of category-B exercise tended to stabilize 3 to 10 minutes after exercise; however, mean lactate concentrations measured during the intervals before and after the peak value differed significantly. The lactate concentration returned to pre-exercise levels within 20 minutes after exercise bouts of category C, but remained above pre-exercise levels up to 60 minutes after bouts of category-A and -B exercise. It was concluded that, for an evaluation of lactate data after intensive anaerobic exercise, sequential blood sampling at 2-minute intervals for a period of up to 12 minutes after exercise is necessary. Less frequent sampling may be a reason for the often described irreproducibility of lactate concentrations in horses. After aerobic or mild anaerobic exercise, one sample is sufficient, but it has to be taken as soon as possible after exercise.  相似文献   

A new synthetic, selective, liquid medium for determination of coliform bacteria     

G Szita  G Biró 《Acta veterinaria Hungarica》1986,34(3-4):145-149

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Inwieweit wird die Bienenzucht durch die Verwendung zuckerhaltiger Pflanzenschutzmittel gefährdet?     

Dr. G. Götze 《Journal of pest science》1929,5(6):73-75

Ohne Zusammenfassung  相似文献   

Geheimrat Dr. Wappes — 80 Jahre alt     

Dr. Künkele 《European Journal of Forest Research》1940,62(1):22-24

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Leistungsprüsung und Rationalisierung     

Dr. e. h. W. Hohenadl 《European Journal of Forest Research》1940,62(1):1-9

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Bücheranzeigen     

Funk  Fabricius  R. Lrendelenburg  B. Mantel  Dr. Rohmeder  K. Escherich  Lukinger 《European Journal of Forest Research》1940,62(8):182-187

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