Seroprevalence of T. gondii in sheep from Marrakech, Morocco     

Sawadogo P  Hafid J  Bellete B  Sung RT  Chakdi M  Flori P  Raberin H  Hamouni IB  Chait A  Dalal A 《Veterinary parasitology》2005,130(1-2):89-92

Toxoplasma gondii is a ubiquitous intracellular protozoan parasite transmitted by food. Concerning this parasite, there are few studies done in Morocco. In this study, 261 sera from sheep intended for consumption in Marrakech were subjected to the Toxoplasma ELISA based serology test for the detection of anti-T. gondii specific IgG confirming a past infection. Of the total tested 72 (27.6%) sera were positive for IgG. This result shows that the seroprevalence approaches the world average and is similar to what is found in other cities of Morocco. This has prompted us to investigate other animal species in the region in order to evaluate the degree of contamination by this parasite as well as the potential risk incurred on consumption of their meat.  相似文献   

Cutaneous lymphoma in a juvenile dog     

Choi US  Jeong SM  Kang MS  Jung IS  Kim DY  Lee CW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(1):47-49

Abstract: An 18-month-old male Doberman Pinscher was referred to the Veterinary Medical Teaching Hospital of the College of Veterinary Medicine for an erythemic nodular mass on the right forelimb. The mass was diagnosed as cutaneous lymphoma, based on cytologic examination of a mass aspirate and histopathology. Using immunohistochemistry the neoplastic cells were positive for CD3 but negative for CD79a, E-cadherin, and pancytokeratin, confirming their origin as T lymphocytes. No tumor recurrence was noted 18 months after surgery. To our knowledge, this is the first report of a solitary nodular form of cutaneous lymphoma in a young dog.  相似文献   

Effects of novel vaccine/adjuvant complexes on the protective immunity against Eimeria acervulina and transcriptome profiles     

Kim DK  Lillehoj HS  Lee SH  Dominowski P  Yancey RJ  Lillehoj EP 《Avian diseases》2012,56(1):97-109

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Development and characterization of mouse monoclonal antibodies reactive with chicken CD83     

Lee SH  Lillehoj HS  Jang SI  Lee KW  Baldwin C  Tompkins D  Wagner B  Del Cacho E  Lillehoj EP  Hong YH 《Veterinary immunology and immunopathology》2012,145(1-2):527-533

This study was carried out to develop and characterize mouse monoclonal antibodies (mAbs) against chicken CD83 (chCD83), a membrane-bound glycoprotein belonging to the immunoglobulin superfamily that is primarily expressed on mature dendritic cells (DCs). A recombinant chCD83/IgG4 fusion protein containing the extracellular region of chCD83 was expressed in Chinese Hamster Ovary (CHO) cells and isolated from the spent cell culture medium by protein G affinity chromatography. The extracellular region of the chCD83 protein was purified and used to immunize mice. A cell fusion was performed, from which 342 hybridomas were screened for mAbs to chCD83. Two mAbs, chCD83-159 and chCD83-227, stained the greatest percentage of chCD83-transfected CHO cells and were selected for further characterization. By flow cytometry, both mAbs reacted with a chicken macrophage cell line, HD11. Both mAbs also recognized a single 53 kDa protein on Western blots of lysates from lipopolysaccharide-stimulated spleen mononuclear cells or unstimulated HD11 cells. Immunostaining of chicken secondary lymphoid organs identified chCD83(+) cells with morphologic and subtissue localization properties comparable to mammalian DCs. In vitro stimulation of spleen mononuclear cells with concanavalin A (Con A) decreased the percentage of chCD83(+) cells compared with cells treated with medium alone. Interestingly, spleen cells treated with Con A in the presence of chCD83-227 mAb exhibited decreased percentage of MHCII(+) cells compared with cells treated with an isotype-matched negative control mAb. These chCD83 mAbs may be useful for future investigations of chicken immune cell maturation and mechanisms of action.  相似文献   

Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues, bone marrow, umbilical cord blood, and Wharton's jelly for treating bone defects     

Byung-Jae Kang  Hak-Hyun Ryu  Sung Su Park  Yoshihisa Koyama  Masanori Kikuchi  Heung-Myong Woo  Wan Hee Kim  Oh-Kyeong Kweon 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(3):299-310

Alternative sources of mesenchymal stem cells (MSCs) for replacing bone marrow (BM) have been extensively investigated in the field of bone tissue engineering. The purpose of this study was to compare the osteogenic potential of canine MSCs derived from adipose tissue (AT), BM, umbilical cord blood (UCB), and Wharton''s jelly (WJ) using in vitro culture techniques and in vivo orthotopic implantation assays. After canine MSCs were isolated from various tissues, the proliferation and osteogenic potential along with vascular endothelial growth factor (VEGF) production were measured and compared in vitro. For the in vivo assay, MSCs derived from each type of tissue were mixed with β-tricalcium phosphate and implanted into segmental bone defects in dogs. Among the different types of MSCs, AT-MSCs had a higher proliferation potential and BM-MSCs produced the most VEGF. AT-MSCs and UCB-MSCs showed greater in vitro osteogenic potential compared to the other cells. Radiographic and histological analyses showed that all tested MSCs had similar osteogenic capacities, and the level of new bone formation was much higher with implants containing MSCs than cell-free implants. These results indicate that AT-MSCs, UCB-MSCs, and WJ-MSCs can potentially be used in place of BM-MSCs for clinical bone engineering procedures.  相似文献   

Chemerin analog regulates energy metabolism in sheep     

Yutaka SUZUKI  Sang‐Houn SONG  Katsuyoshi SATO  Kyoung‐Ha SO  Astrid ARDIYANTI  Shun KITAYAMA  Yeon‐Hee HONG  Sung‐Dae LEE  Ki‐Choon CHOI  Akihiko HAGINO  Kazuo KATOH  Sang‐gun ROH 《Animal Science Journal》2012,83(3):263-267

Accumulating data suggest a relationship between chemerin and energy metabolism. Our group previously described gene cloning, expression analysis and the regulatory mechanism of chemerin and its own receptor in mice and cattle. The objective of the present study was to investigate the physiological effect of chemerin on endocrine changes and energy metabolism in sheep using a biologically stable chemerin analog. The chemerin analog was intravenously administrated (100 or 500 µg/head) to sheep, and plasma insulin and metabolites (glucose, nonesterified fatty acids (NEFA), triglyceride, total cholesterol and high‐density lipoprotein (HDL) cholesterol) were analyzed. The chemerin analog dramatically increased the insulin levels, and glucose levels were decreased. NEFA levels were slightly decreased at 20 min but then increased gradually from 60 to 180 min after analog administration. In addition, injection of the chemerin analog immediately increased triglyceride and total cholesterol but not HDL levels. These results suggested that chemerin analog regulated insulin secretion related to glucose metabolism and the release of triglycerides in sheep in vivo. This study provides new information about endocrine and metabolic changes in response to chemerin in sheep.  相似文献   

Effects of administration of IH901, a ginsenoside intestinal metabolite,on muscular and pulmonary antioxidant functions after eccentric exercise     

Nam-Jin Lee  Jung-Won Lee  Jong-Hwan Sung  Yeoung-Gyu Ko  Seongsoo Hwang  Jong-Koo Kang 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(3):249-256

This study was conducted to investigate whether administration of IH901, a ginseng intestinal metabolite, ameliorates exercise-induced oxidative stress while preserving antioxidant defense capability in rat skeletal muscles and lung. Eight adult male Sprague-Dawley rats per group were randomly assigned to the resting control, exercise control, resting with IH901 (25, 50, and 100 mg/kg) consumption (R/IH901), or exercise with IH901 (25, 50, and 100 mg/kg) consumption (E/IH901) group. The trained groups ran 35 min 2 days/week for 8 weeks. To analyze the IH901-training interaction, serum biochemical analysis, lipid peroxidation, citrate synthase, protein oxidation, antioxidant and superoxide dismutase in skeletal muscles and lung tissue were measured. Compared to the exercise control group, animals that consumed IH901 had significantly increased exercise endurance times (p < 0.05) and decreased plasma creatine kinase and lactate dehydrogenase levels (p < 0.05), while those in the E/IH901 groups had increased citrate synthase and anti-oxidant enzymes and decreased lipid peroxidation and protein oxidation (p < 0.05). In conclusion, IH901 consumption in aging rats after eccentric exercise has beneficial effects on anti-inflammatory and anti-oxidant activities through down-regulation of pro-inflammatory mediators, lipid peroxidation, and protein oxidation and up-regulation of anti-oxidant enzymes.  相似文献   

Apo-9′-Fucoxanthinone,Isolated from Sargassum muticum,Inhibits CpG-Induced Inflammatory Response by Attenuating the Mitogen-Activated Protein Kinase Pathway     

Doobyeong Chae  Zahid Manzoor  Sung Chun Kim  Sohyun Kim  Tae-Heon Oh  Eun-Sook Yoo  Hee-Kyoung Kang  Jin-Won Hyun  Nam Ho Lee  Mi-Hee Ko  Young-Sang Koh 《Marine drugs》2013,11(9):3272-3287

Sargassum muticum (S. muticum) is a brown edible alga and widely distributed in Korea. This report was designed to evaluate the anti-inflammatory properties of apo-9′-fucoxanthinone (APO-9′) isolated from S. muticum on pro-inflammatory cytokine production. S. muticum extract (SME) exhibited significant inhibitory effects on pro-inflammatory cytokine production in bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs). APO-9′ pre-treatment in the CpG DNA-stimulated BMDMs and BMDCs showed a strong dose-dependent inhibitory effect on interleukin (IL)-12 p40, IL-6 and tumor necrosis factor (TNF)-α production with IC₅₀ values ranging from 5.31 to 13.79. It exhibited a strong inhibitory effect on the phosphorylation of ERK1/2 and on activator protein (AP)-1 reporter activity. APO-9′ pre-treatment exhibited significant inhibition of CpG DNA-induced production of inducible nitric oxide synthase. Taken together, these data suggest that SME and APO-9′ have a significant anti-inflammatory property and warrant further studies concerning the potentials of SME and APO-9′ for medicinal use.  相似文献   

Discovery of New Eunicellin-Based Diterpenoids from a Formosan Soft Coral Cladiella sp.     

Tsung-Hung Chen  Mei-Chin Lu  Yu-Chia Chang  Yin-Di Su  Yu-Hsin Chen  Nai-Cheng Lin  Lee-Shing Fang  Yang-Chang Wu  Ping-Jyun Sung 《Marine drugs》2013,11(11):4585-4593

A new eunicellin diterpenoid, cladieunicellin I (1), and a new natural eunicellin, litophynin I diacetate (2), were isolated from a Formosan soft coral identified as Cladiella sp. The structures of eunicellins 1 and 2 were elucidated by spectroscopic methods and by comparison of the spectral data with those of related analogues. Eunicellin 1 exhibited significant cytotoxicity toward the DLD-1 human colorectal adenocarcinoma cells.  相似文献   

Improving efficiency of sow productivity： nutrition and health   总被引：2，自引：0，他引：2  

Sung Woo  Kim Alexandra  C  Weaver Yan  Bin Shen  Yan Zhao 《畜牧与生物技术杂志(英文版)》2013,(3):187-194

This reviews research focused to understand the nutrient requirement and balance to meet the needs of fetal growth,mammary growth,and milk production.This summary will handle how feeding strategies can be adjusted according to the nutrient needs for a sow to enhance productivity and health.Most research data used in this summary are based on the studies conducted by the authors between 1996 and 2013.Nutrient requirements of sows are affected by stage of gestation and parity of sows.Dietary antioxidant concentrations need to be re-evaluated for its sufficiency in sow diets especially to prevent excessive oxidative stress during late gestation and lactation.When feeding sows,consideration of phase feeding of gestating sows and parity feeding of lactating sows could enhances production longevity and health of sows.Use of selected nutrients and additives seems to help productivity and health of sows.  相似文献