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Journal of Crop Science and Biotechnology - Lycium barbarum (Solanaceae), commonly known as Goji (or wolfberry), is popular for its nutritive and medicinal properties and is called a “super...  相似文献   
64.
A method for solid phase extraction of trace metals, namely, Cd, Cu, and Ni, using Amberlite XAD-2 functionalized with a new chelating ligand, 2-hydroxy-acetophenone-3-thiosemicarbazone (Amberlite XAD-2-HAPTSC), has been developed. The optimum experimental conditions for the quantitative sorption of three metals, pH, effect of flow rate, concentration of eluent, sorption capacity, kinetics of sorption, and the effect of diverse ions on the sorption of analytes have been investigated. The chelating resin could be reused for more than 20 cycles of sorption-desorption without any significant change (<1.5%). The accuracy of the proposed procedure was evaluated by standard reference materials. The proposed method was applied for the determination of trace metal ions in leafy vegetable samples collected from different sources and medicinal plant leaves.  相似文献   
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Many maternally inherited and incurable neuromyopathies are caused by mutations in mitochondrial (mt) transfer RNA (tRNA) genes. Kinetoplastid protozoa, including Leishmania, have evolved specialized systems for importing nucleus-encoded tRNAs into mitochondria. We found that the Leishmania RNA import complex (RIC) could enter human cells by a caveolin-1-dependent pathway, where it induced import of endogenous cytosolic tRNAs, including tRNA(Lys), and restored mitochondrial function in a cybrid harboring a mutant mt tRNA(Lys) (MT-TK) gene. The use of protein complexes to modulate mitochondrial function may help in the management of such genetic disorders.  相似文献   
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BACKGROUND: Nanoemulsion composed of neem oil and non‐ionic surfactant Tween 20, with a mean droplet size ranging from 31.03 to 251.43 nm, was formulated for various concentrations of the oil and surfactant. The larvicidal effect of the formulated neem oil nanoemulsion was checked against Culex quinquefasciatus. RESULTS: O/W emulsion was prepared using neem oil, Tween 20 and water. Nanoemulsion of 31.03 nm size was obtained at a 1:3 ratio of oil and surfactant, and it was found to be stable. The larger droplet size (251.43 nm) shifted to a smaller size of 31.03 nm with increase in the concentration of Tween 20. The viscosity of the nanoemulsion increased with increasing concentration of Tween 20. The lethal concentration (LC50) of the nanoemulsion against Cx. quinquefasciatus was checked for 1:0.30, 1:1.5 and 1:3 ratios of oil and surfactant respectively. The LC50 decreased with droplet size. The LC50 for the ratio 1:3 nanoemulsions was 11.75 mg L?1. CONCLUSION: The formulated nanoemulsion of 31.03 nm size was found to be an effective larvicidal agent. This is the first time that a neem oil nanoemulsion of this droplet size has been reported. It may be a good choice as a potent and selective larvicide for Cx. quinquefasciatus. Copyright © 2011 Society of Chemical Industry  相似文献   
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A field experiment was conducted to investigate the effects of 1,2,3,4,5,6-hexachlorocyclohexane (BHC), phorate, carbofuran, and fenvalerate, at their recommended doses, on some chemical and microbiological properties of the rhizosphere soil in relation to rice yields. In general, the insecticides had a beneficial effect on rhizosphere soil properties. Carbofuran strongly stimulated the mineralization of organic C. BHC and phorate led to the retention of less total N in the soil. BHC released more NH inf4 sup+ -N than the other insecticides. Phorate, however, liberated the most NO inf3 sup- -N. Phorate and fenvalerate released more available P than BHC and carbofuran did. All the insecticides stimulated the proliferation of aerobic non-symbiotic N2-fixing and phosphate-solubilizing microorganisms, resulting in an overall increase in rice yield. BHC had the greatest effect on rice yields, followed by phorate.  相似文献   
68.
Sitostanol has been converted in high to near-quantitative extent to the corresponding long-chain acyl esters via esterification with oleic acid or transesterification with methyl oleate or trioleoylglycerol using immobilized lipases from Rhizomucor miehei (Lipozyme IM) and Candida antarctica (lipase B, Novozym 435) as biocatalysts in vacuo (20-40 mbar) at 80 degrees C, whereas the conversion was markedly lower at 60 and 40 degrees C. Corresponding conversions observed with papaya (Carica papaya) latex lipase were generally lower. High conversion rates observed in transesterification of sitostanol with methyl oleate at 80 degrees C using Lipozyme IM were retained even after 10 repeated uses of the biocatalyst. Saturated sterols such as sitostanol and 5alpha-cholestan-3beta-ol were the preferred substrates as compared to Delta(5)-unsaturated cholesterol in transesterification reactions with methyl oleate using Lipozyme IM. Transesterification of cholesterol with dimethyl 1,8-octanedioate using Lipozyme IM in vacuo yielded methylcholesteryl 1,8-octanedioate (75%) and dicholesteryl 1,8-octanedioate (5%). However, transesterification of cholesterol with diethyl carbonate and that of oleyl alcohol with ethylcholesteryl carbonate, both catalyzed by Lipozyme IM, gave ethylcholesteryl carbonate and oleylcholesteryl carbonate, respectively, in low yield (20%). Moreover, cholesterol was transesterified with ethyl dihydrocinnamate using Lipozyme IM to give cholesteryl dihydrocinnamate in moderate yield (56%), whereas the corresponding reaction of lanosterol gave lanosteryl oleate in low yield (14%).  相似文献   
69.
Waxy wheat (Triticum aestivum L.) contains endosperm starch lacking in amylose. To realize the full potential of waxy wheat, the pasting properties of hard waxy wheat flours as well as factors governing the pasting properties were investigated and compared with normal and partial waxy wheat flours. Starches isolated from six hard waxy wheat flours had similar pasting properties, yet their corresponding flours had very different pasting properties. The differences in pasting properties were narrowed after endogenous α‐amylase activity in waxy wheat flours was inhibited by silver nitrate. Upon treatment with protease, the extent of protein digestibility influenced the viscosity profile in waxy wheat flours. Waxy wheat starch granules swelled extensively when heated in water and exhibited a high peak viscosity, but they fragmented at high temperatures, resulting in more rapid breakdown in viscosity. The extensively swelled and fragmented waxy wheat starch granules were more susceptible to α‐amylase degradation than normal wheat starch. A combination of endogenous α‐amylase activity and protein matrix contributed to a large variation in pasting properties of waxy wheat flours.  相似文献   
70.
Summary A survey of 101 farmers with sows was conducted in the Koshi Hills of Nepal. Estimates of those reproductive traits which did not differ significantly between local and Pakhribas crossbred sows, are farrowing interval 7·4 months, litter size at birth 8·3, age of piglets at weaning 2·2 months and total number of farrowings 13·2. Local pigs reach sexual maturity sooner than Pakhribas crossbreds; age at first farrowing for the two genotypes is 10·7 and 12·0 months (P<0·05), and age at first mating of boars is 6·5 and 7·4 months (P<0·05). Sows farrow throughout the year and there is no clearly defined best season for farrowing. Pre-weaning mortality rates are estimated to be 22% for local piglets and 15% for Pakhribas crossbreds. Almost all farmers practise controlled mating in which either the sow is taken to the boar or the boar is brought to the sow. The proportion of farmers (with sows) who own a boar is only about 5 per cent.
Resumen Se llevó a cabo un estudio de 101 porquerizas en las alturas Koshi de nepal. Los índices zooténicos reproductivos, que no difirieron significativamente entre hembras locales y cruzadas Pakhribas fueron, intervalo entre partos 7·4 meses, tama?o de la camada al nacimiento 8·3, edad de los cerdos al destete 2·2 meses y número total de partos 13·2. Los cerdos locales alcanzaron la madurez sexual más rápido que los cruzados. La edad al primer parto de los dos genotipos fue de 10·7 y 12·0 meses (P<0·05), y la edad a la primera monta de los machos fue de 6·5 y 7·4 meses (P<0·05). No hubo una estación del a?o definida para la parición. La mortalidad antes del destete se estimó en 22% para los cerdos locales y en 15% para los cruces. Casi todos los cerdos practican monta controlada, en donde la hembra es llevada al macho, o el macho a la hembra. La proporción de finqueros que tienen reproductor es de apenas cinco por ciento.

Résumé Une enquête portant sur 101 fermiers possédant des truies a été conduite dans les collines de Koshi au Népal. Les caractéristiques estimées de ces reproductions, qui ne diffèrent pas significantivement entre les truies de race locale et les croisements Pakhribas, sont pour l’intervalle de mise bas de 7,4 mois, 8,3 porcelets par portée, 2,2 mois pour l’age au sevrage et un nombre total de mise bas de 13,2. Les porcs de race locale atteignent la maturité sexuelle plus t?t que les croisements Pakhribas. L’age à la première mise bas pour les deux génotypes est de 10,7 et 12,0 mois (P<0,05), l’age au premier accouplement pour les verrats est de 6,5 et 7,4 mois pourP<0,05, respectivement. Les truies mettent bas toute l’année et il n’existe pas, à cet égard, de saison optimale clairement individualisée. Les taux de mortalité avant sevrage sont estimés à 22 p. 100 pour la race locale et à 15 p. 100 pour les croisements Pakhribas. Presque tous les fermiers pratiquent le contr?le des saillies pendant lequel la truie est conduite au verrat ou inversement. La proportion des fermiers ayant des truies et possédant aussi des verrats n’est que de 5 p. 100.
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