Morphological disparities in the epidermal and anatomical features of the leaf among wild Brassica juncea populations     

CHAOHE HUANGFU  XIAOLING SONG  SHENG QIANG 《Weed Biology and Management》2009,9(3):234-242

In the present article, we investigated the epidermal and anatomical features of 10 wild Brassica juncea populations with different susceptibilities to glyphosate in detail. The characteristics of the leaf surface and anatomy could affect the efficacy of glyphosate uptake in weeds through the influence of its adherence and penetration. Our results showed that herbicide-resistant wild populations generally had greater leaf thickness, adaxial cutin thickness, and trichome density in the upper epidermis, but lower spongy tissue thickness and vascular bundle numbers, as compared with the susceptible populations, suggesting that differences in morphological traits among populations might contribute to their variable response to glyphosate. The possible morphological involvement in the differential susceptibility of wild B. juncea populations to glyphosate also was discussed.  相似文献   

野生大豆GsADF1基因的克隆与表达分析   总被引：1，自引：0，他引：1  

何慧  丁一琼  汪潇琳  喻德跃 《大豆科学》2009,28(5)

ADF蛋白是一种在真核生物中广泛存在的低分子量的肌动蛋白结合蛋白,在细胞分裂、细胞运动、植物顶端生长如花粉管伸长,根毛的形成等重要的生命活动中发挥着重要的作用.以野生大豆(Glycine soja)的叶片cDNA为模板,通过RT-PCR方法扩增分离获得GsADF1基因.该基因全长为693 bp,包含417 bp的开放阅读框;其编码的ADF蛋白含139个氨基酸残基.运用生物信息学方法将GsADF1与其他物种ADF蛋白氨基酸序列进行多重比对并构建系统进化树,发现GsADF1与其它生物的ADF蛋白具有很高的同源性.这说明植物ADF基因高度保守.为进一步研究GsADF1在野生大豆不同组织,器官以及不同发育期大豆种子中的表达情况,对它进行了荧光定量分析(Real time RT-PCR).结果表明:GsADF1在野生材料的根、茎、叶、花和种子中都有表达,且以根和花中表达量最高.而对其在不同发育阶段的种子中的分析表明该基因在15DAF、20DAF、30DAF、40DAF和45DAF的种子中都有表达,且以15DAF表达量最低,随后呈上升趋势.其表达量的变化表明GsADFI通过调节肌动蛋白可能参与了种子从胚胎发生到贮藏物质积累的转变.  相似文献   

关于对水土保持方案编审工作规范化几个问题的探讨     

张小林 《中国水土保持》2009,(8):18-19

近年来随着生产建设项目的增多和水土保持编报率的提高,在水土保持方案编审中出现了编制资质证书管理不严格、会议审查单位组织不严谨、方案审查专家管理不规范、方案编制套用严重、方案限批不够、水保监测二次许可等诸多问题,已影响到了水行政主管部门的公共形象。各级水行政主管部门应当引起重视,进一步加强规范管理,不断提高水土保持方案的编制质量,为水土保持监督管理提供坚实的依据。  相似文献   

日本落叶松在旺苍县引种栽培初步研究     

罗小林  万云 《四川林勘设计》2003,(1):10-13

通过对旺苍县盐井河采育场10多年来引种栽培日本落叶松的结果与旺苍县外其它地区的引种栽培试验结果和本地华山松的生长情况进行对比分析，证明引种是成功的，进一步探讨了日本落叶松在旺苍的引种效果和生态适应性，提出了旺苍自然地理分布的引种适生区，是旺苍北部中山区主要速生造林树种之一。  相似文献   

四川主要景观植物有性繁殖技术     

曾其国  刘应高  蒋小林 《四川林业科技》2007,28(1):128-131

本文结合四川景观植物的生产现状,从品种选择、种子贮藏、播种技术以及育苗管理四个方面阐述了主要景观植物有性繁殖技术,旨在为生产提供服务。  相似文献   

3种阔叶树育苗技术及苗木生长规律研究初探   总被引：2，自引：0，他引：2  

刘志强  肖水清  邹元熹  肖才发 《江西林业科技》2001,(3):14-15

通过对闽楠，蓝果树，东京野茉莉的育苗试验，研究苗木生长规律，掌握生理生物学特性。  相似文献   

家具检测用环境舱恒温恒湿系统方案改进     

张天鹏  司慧  颜小林  樊超 《林业机械与木工设备》2015,(4)

对所研制的第一代环境舱恒温恒湿系统进行实验及分析,分析环境舱内壁结霜凝水以及检测甲醛和TVOC不够准确等问题产生的原因,并提出具有针对性的改进方案。实验表明,改进后的系统可有效解决上述问题,能够精确控制环境舱内部温湿度的恒定,从而保证环境舱在工作过程中对样品分析结果的准确性。  相似文献   

不同类农作物秸秆在肉羊瘤胃中的降解特性研究     

陈晓琳  孙娟  王月超  马涛  王海超  刁其玉 《中国畜牧杂志》2015,(5):45-51

本试验旨在研究不同类农作物秸秆在肉羊瘤胃中的降解规律,以3头安装有永久性瘤胃瘘管的杜×寒肉用绵公羊为试验动物,以青贮玉米秸为对照材料,采用半体内尼龙袋法对不同产地的6类19个秸秆样品干物质(DM)和中性洗涤纤维(NDF)的降解率和降解参数进行测定。结果表明:秸秆DM和NDF的瘤胃降解规律相近,48 h后趋于稳定,72 h消失率和有效降解率(ED)最高的为藤蔓类的地瓜秧和花生秧,接近青贮玉米秸,玉米秸、大豆秸和稻草秸较低,小麦秸最低(P0.05);DM和NDF有效降解率与粗蛋白质(CP)、NDF和酸性洗涤纤维(ADF)的相关性较强(P0.01),拟合方程分别为DMD=0.9900+0.3270 CP-0.8099 NDF-0.1880 ADF(R~2=0.8860,P0.0001)、NDFD=0.5111+0.3833 CP-0.1519 NDF-0.3963 ADF(R~2=0.7585,P0.0001)。地瓜秧和花生秧在瘤胃中降解快,可作为肉羊较好的饲料来源,而玉米秸、大豆秸、稻草秸和小麦秸在瘤胃中降解效果较差。  相似文献   

Sexual compatibility of transgenic soybean and different wild soybean populations          下载免费PDF全文

HU Yu-qi  SHENG Ze-wen  LIU Jin-yue  LIU Qi  QIANG Shen  SONG Xiao-ling  LIU Biao 《农业科学学报》2022,21(1):36-48

The introduction of genetically modified (GM) soybean into farming systems raises great concern that transgenes from GM soybean may flow to endemic wild soybean via pollen. This may increase the weediness of transgenic soybean by increasing the fitness of hybrids under certain conditions and threaten the genetic diversity of wild soybean populations. Although pollen-mediated gene flow between GM crops and wild relatives is dependent on many factors, the sexual compatibility (SC) determined by their genetic backgrounds is the conclusive factor. The considerable genetic variation among wild soybean populations may cause compatibility differences between different wild and cultivated soybeans. Thus, an evaluation of the SC between transgenic soybean and different wild soybeans is essential for assessing the environmental consequences of cultivated soybean–wild soybean transgene flow. The podding and seed sets were assessed after artificial hybridization using transgenic glyphosate-resistant soybean as the paternal parent and 18 wild soybean populations as the maternal parents. Then, the average number of filled seeds produced in 200 flowers (AFS) was calculated for each wild soybean under natural self-pollination as well as under artificial crossing with transgenic soybean. Finally, the index of cross-SC was calculated (ICSC) as the ratio of the AFS of wild soybean artificially crossed with transgenic soybean and the AFS of naturally self-pollinated wild soybean. The results demonstrated that after self-pollination and crossing with transgenic soybean, the average podding rates of 18 wild soybean populations ranged within 96.50–99.50% and 4.92–18.03%, and the average filled seed numbers per pod varied from 1.70 to 2.69 and 0.20 to 0.48, respectively. The results showed that approximately 89% of wild soybeans displayed either medium or higher than medium SC with transgenic soybean (ICSC>1.0%). This implied the high possibility of gene flow via pollen from transgenic soybean to wild soybean.  相似文献   

中华按蚊几丁质酶基因家族的全基因组鉴定与分析     

刘燕  邱品品  刘蒨莹  向凯  杨小林  乔梁 《蚕学通讯》2022,42(1):22-29

本研究基于生物信息方法对中华按蚊的几丁质酶基因家族进行全基因组鉴定与分析.利用BLAST、HMM及Softberry网站中的基因预测程序进行序列鉴定、检验及补充,筛选出23个中华按蚊几丁质酶基因及类似基因,并进行系统发生分析,将中华按蚊几丁质酶基因分为八大家族,其外显子数为2～13个,跨度较大;基因分布分析结果表明As...  相似文献