Foot-and-mouth disease eradication efforts in the Republic of Korea   总被引：3，自引：0，他引：3       下载免费PDF全文

Yi-Suk Joo  Soo-hwan An  Ok-Kyung Kim  Juan Lubroth    Jung-Hyang Sur 《Canadian journal of veterinary research》2002,66(2):122-124

On March 20, 2000, a suspected vesicular disease in cattle was reported to the National Veterinary Research and Quarantine Service (NVRQS) of the Republic of Korea. This represented the index case of a foot-and-mouth disease (FMD) outbreak, which spread through several provinces. The Republic of Korea had been free of FMD for 66 years prior to the reintroduction of the virus and had recently suspended imports of pork and pork products from neighboring Japan owing to a reported FMD outbreak in that country. The Korean outbreak was ultimately controlled through the combination of preemptive slaughter, animal movement restrictions, and a strategy of ring vaccination. The purpose of this paper is to review the current FMD situation in Korea in the aftermath of its 2000 epizootic and how it may affect future efforts to eradicate or reduce risk of reintroduction of the disease into Korea.  相似文献   

萘乙酸对大白菜钙素吸收运转及防治干烧心病的研究   总被引：10，自引：1，他引：9  

闻凤英  孙德岭 《园艺学报》1991,18(2):148-152

本研究从补钙入手,探索防治干烧心病的方法。用0.7％CaCl_2·2H_2O对和50ppmNAA混合液在大白菜结球期叶面喷洒5次,经8年试验,对大白菜干烧心病的相对防治效果达80％以上。 在此基础上,采用~(45)Ca示踪试验的方法对防病机理做了进一步研究。结果表明,无论从根部还是从叶部加萘乙酸对根部和叶部~(45)Ca的吸收和运转均具有促进作用,并且改变了其在大白菜植株内的分配规律。对照株单位干叶重dpm值自外向内呈下降趋势(130×10~3─60×10~3);用NAA处理株则相反,自外向内呈增加趋势(120×10~3─250×10~3),说明萘乙酸具有促进钙素向叶球中内部运转的功效。这种效果在贮藏期间仍能保持。另外,~(45)Ca向直接受到NAA处理部位的运转量有明显增高的趋势。这一结果为补钙防治干烧心病提供了理论依据。  相似文献   

学会改革与发展面临的问题及对策     

安凯春 《勤云标准版测试》2005,(7)

随着社会主义经济体制的逐步完善,改革的不断深化和对外开放的进一步扩大,学会在组织体制、管理模式、运行机制和活动方式等方面,还不能完全适应社会主义市场经济的要求,在综合分析学会存在诸多问题以及面临激烈竞争和严峻挑战的基础上,针对农业学会目前现状,提出了对策,以及今后努力发展的方向。  相似文献   

‘Plantibodies’: a flexible approach to design resistance against pathogens     

A. Schots  J. De Boer  A. Schouten  J. Roosien  J. F. Zil Verentant  H. Pomp  L. Bouwman-Smits  H. Overmars  F. J. Gommers  B. Visser  W. J. Stiekema  J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   

Characteristics of inapparent Aleutian disease virus infection in mink.     

S H An  F J DePauli  P Wright  D G Ingram 《Research in veterinary science》1978,24(2):200-204

Inapparent of nonprogressive Aleutian disease virus (ADV) infection is a subclinical but persistent virus infection of mink. Mink with the inapparent type of ADV infection when subjected to stress did not develop the progessive form of the disease. However, when challenged with a large dose of the virus, these mink did develop progressive Aleutian disease indicating that they were not highly resistant to the virus. Sera of mink with either the progressive of the inapparent type of ADV infection did not neutralise the virus. The anti-ADV antibody activity in mink with inapparent type of ADV infection was in the IgG fraction of the serum the same as in mink with progressive Aleutian disease. These data indicate that the resistance of the mink with inapparent infection as compared to mink with progressive Aleutian disease was not due to a difference in the class of immunoglobulin response to the virus. However, mink with progressive Aleutian disease showed a greatly increased immunoglobulin response.  相似文献   

‘三棱榄''橄榄果实香气成分分析   总被引：7，自引：1，他引：7  

钟明  陈玉芬  甘廉生  蔡时可 《园艺学报》2003,30(6):757-761

1 材料与方法选取广东优良鲜食橄榄品种‘三棱榄’,2001年12月6日采样,采用固相微萃取法(SPME)富集香气成分(鲜橄榄果肉于15℃下捣碎后取样1.0 g放入4 mL聚四氟乙烯硅橡胶垫密封螺口玻璃瓶中,插入100μm聚二甲基硅氧烷纤维头于室温25-30℃顶空取样2 h),用美国Finnigan TRACE GC-MS气相色谱-质谱联用仪进行分析。气相色谱柱为DB-1弹性毛细管柱30 m×0.25 mm,载气为He(99.99％),流速1.0mL/min。程序升温从40℃开始先保持10 min,后以2℃/min的升温速率升至150℃保持10 min。质谱条件:电子能量70 eV,离子源温度250℃,质量范围35-450 aum,不分流进样。2002年12月18日采样重复分析。  相似文献   

Characteristics for Practical Use of Attenuated Isolate UA-Fukushima of <Emphasis Type="Italic">Tomato mosaic virus</Emphasis>     

Tsutomu MATSUMOTO  Yuichiro NARA  Hiromitsu FURUYA  Harumi TAKAHASHI  Kiichi TAIRAKO  Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384

L₁₁A-Fukushima (L₁₁A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited. Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L₁₁A-F and L₁₁A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these results, L₁₁A-F appears to possess the properties necessary for practical use. To manage L₁₁A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L₁₁A-F preparations was established. Received 10 June 2002/ Accepted in revised form 30 October 2002  相似文献   

Effects of Latent Infection of Stock Plants and Abundance of Thrips on the Occurrence of <Emphasis Type="Italic">Tomato spotted wilt virus </Emphasis>in Chrysanthemum Fields     

Shohei MATSUURA  Shigeru HOSHINO  Hideaki HAYASHI  Tetsuyuki KOHGUCHI  Kyoji HAGIWARA  Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102

DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV originating from latently infected stock plants in chrysanthemum production fields. Received 27 July 2001/ Accepted in revised form 27 November 2001  相似文献   

SeGLP-1的分离纯化及硒活性结构分析   总被引：8，自引：0，他引：8  

尚德静  王孝敏  许永廷  田星  安悦 《食用菌学报》2002,9(1):22-27

灵芝（Ganoderma lucidum)加硒深层培养后的菌丝（含Se1600μg/g)分别经水及碱水提取，醇析，透析，Sevage法脱蛋白，DEAE－cellulose柱层析纯化，得SeGLP-1。经SephadexG－100，聚丙烯酰胺凝胶电泳和紫外光谱分析鉴定，SeGLP-1为均一级分。GC与TLC分析表明，SeGLP-1含Gal,Glu,Xyl,Rha,Man，其单糖摩尔比为Gal:Glu:Xyl:Rha:Man=0.23:1.00:0.72:0.25:0.09。SeGLP-1硒含量1642μg/g。SeGLP-1中Se活性中心的结构可能为O＝Se=O,即Se取代了灵芝多糖GLP－1中－OCH3结构上的CH3，从而形成了硒氧双键结构。  相似文献   

Distribution of citrus viroids and <Emphasis Type="Italic">Apple stem grooving virus</Emphasis> on citrus trees in Japan using multiplex reverse transcription polymerase chain reaction     

Takao Ito  Nobuyuki Namba  Tsutae Ito 《Journal of General Plant Pathology》2003,69(3):205-207

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