Effect of Germination and Fermentation Process on the Antioxidant Compounds of Quinoa Seeds     

Ramiro Ariel Carciochi  Leandro Galván-D’Alessandro  Pierre Vandendriessche  Sylvie Chollet 《Plant foods for human nutrition (Dordrecht, Netherlands)》2016,71(4):361-367

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Pericardial mesothelioma in a Bengal tiger (Panthera tigris).     

Ellen B Wiedner  Ramiro Isaza  William A Lindsay  Allison L Case  Joshua Decker  John Roberts 《Journal of zoo and wildlife medicine》2008,39(1):121-123

A 17-year-old Bengal tiger (Panthera tigris) presented with dyspnea and tachypnea. Radiographs revealed severe pleural and pericardial effusion, but no obvious mass. During attempts to remove the fluid under anesthesia, the cat developed cardiac tamponade and died. At necropsy, a nodular mass was found at the heart base and was identified as a pericardial mesothelioma. This is the first report of this tumor in any large cat.  相似文献   

Dog sperm DNA: Raw semen evaluation with Toluidine blue stain     

Norma E. Monachesi  Deborah Neild  Maria Carretero 《Reproduction in domestic animals》2019,54(8):1078-1084

The toluidine blue (TB) stain has been used in different species to evaluate the degree of chromatin condensation. The objectives of this study were as follows: simplify the TB stain to evaluate sperm in canine raw semen, verify the staining patterns for this species using this simplified technique and establish a protocol for using dithiothreitol (DTT) as a positive control for TB staining in dogs. Twenty‐one ejaculates were collected from 7 adult male dogs; semen was extended, fixed with ethanol 96° and stained with TB using 2 staining times: 15 and 30 min. In addition, 3 incubation times with 1% DTT were assayed (2, 5 and 30 min). Three staining patterns were established: light blue colouring (TB negative, normal chromatin condensation), light violet (TB intermediate, some degree of chromatin decondensation) and dark blue‐violet (TB positive, high degree of chromatin decondensation). No significant differences (p > 0.05) were observed between the staining times (15 and 30 min) for any of the TB patterns. All DTT incubation times (2, 5 and 30 min) showed 100% sperm positive to TB. To conclude, it was possible to simplify the TB stain and determine the different patterns in canine spermatozoa. Also, DTT can be used both as a positive control for the stain and to evaluate individual susceptibility to decondensation in vitro.  相似文献   

Improving xanthophyll extraction from marigold flower using cellulolytic enzymes   总被引：1，自引：0，他引：1  

Navarrete-Bolaños JL  Jiménez-Islas H  Botello-Alvarez E  Rico-Martínez R  Paredes-López O 《Journal of agricultural and food chemistry》2004,52(11):3394-3398

In this work is studied the effect of a noncommercial enzyme preparation on xanthophyll extraction from marigold flower (Tagetes erecta). The enzymatic extract was synthesized by endogenous microorganisms previously isolated and identified as Flavobacterium IIb, Acinetobacter anitratus, and Rhizopus nigricans. The results show that the extraction yield depends directly on the extent of the enzymatic hydrolysis of cell walls in the flower petals and that it is possible to reach yields in excess of those previously reported for treatments with commercially available enzymes (29.3 g/kg of dry weight). HPLC analysis of the product indicates that the original xanthophyll profile is not altered. The enhanced extraction system appears to be very competitive when compared to the traditional process and current alternatives.  相似文献   

Technical Note: Coccygeal vein catheterization for sampling of reproductive tract-derived products from the uterine–ovarian drainage     

Sydney T Reese  Gessica A Franco  Ramiro V Oliveira Filho  Reinaldo F Cooke  Michael F Smith  Ky G Pohler 《Journal of animal science》2021,99(2)

Blood sample collection from the caudal vena cava at the site of uterine–ovarian drainage provides a more exact evaluation of the concentration and pattern of secretion of uterine or ovarian secreted products for studies of reproductive processes in cyclic and pregnant cattle compared with samples collected from general circulation. This paper describes a thorough and updated procedure for cannulating the coccygeal vein into the caudal vena cava for the collection of serial blood samples at or near the site of uterine–ovarian drainage. Concentrations of progesterone were quantified in cows of different reproductive tract sizes with an active corpus luteum to assess the distance for proper catheter placement compared with circulating concentrations collected from the jugular vein. This procedure has a low risk for side effects, can be used effectively in pregnant animals with no major consequence to the viability of the pregnancy, and provides means for frequent collections up to 12 d.  相似文献   

Defective telomere lagging strand synthesis in cells lacking WRN helicase activity     

Crabbe L  Verdun RE  Haggblom CI  Karlseder J 《Science (New York, N.Y.)》2004,306(5703):1951-1953

Cells from Werner syndrome patients are characterized by slow growth rates, premature senescence, accelerated telomere shortening rates, and genome instability. The syndrome is caused by the loss of the RecQ helicase WRN, but the underlying molecular mechanism is unclear. Here we report that cells lacking WRN exhibit deletion of telomeres from single sister chromatids. Only telomeres replicated by lagging strand synthesis were affected, and prevention of loss of individual telomeres was dependent on the helicase activity of WRN. Telomere loss could be counteracted by telomerase activity. We propose that WRN is necessary for efficient replication of G-rich telomeric DNA, preventing telomere dysfunction and consequent genomic instability.  相似文献   

Pharmacokinetics of magnesium and its effects on clinical variables following experimentally induced hypermagnesemia     

Stephen A. Schumacher  Ramiro E. Toribio  Brian Scansen  Jeffrey Lakritz  Alicia L. Bertone 《Journal of veterinary pharmacology and therapeutics》2020,43(6):577-590

The objectives of this study were to describe pharmacokinetic and pharmacodynamic changes as a result of a single intravenous administration of magnesium sulfate (MgSO₄) to healthy horses. MgSO₄ is a magnesium salt that has been used to calm horses in equestrian competition and is difficult to regulate because magnesium is an essential constituent of all mammals. Six healthy adult female horses were administered a single intravenous dose of MgSO₄ at 60 mg/kg of body weight over 5 min. Blood, urine, and cerebrospinal fluid (CSF) samples were collected, and cardiovascular parameters were monitored and echocardiograms performed at predetermined times. Noncompartmental pharmacokinetic analysis was applied to plasma concentrations of ionized magnesium (Mg²⁺). Objective data were analyzed using the Wilcoxon rank-sum test with p < .05 used as a determination for significance. Plasma concentrations of Mg²⁺ increased nearly fivefold, ionized calcium (Ca²⁺) decreased by nearly 10%, and the Ca²⁺ to Mg²⁺ ratio declined more than 3.5-fold and remained different than baseline until 24 hr (p < .05). Significant changes were seen with urinary fractional excretion of electrolytes, cardiovascular parameters, and echocardiographic measurements. No changes were detected in CSF electrolyte concentrations. The decrease in Ca²⁺ result of hypermagnesemia supports the interaction between these cations. Alterations detected in plasma electrolyte concentrations and urinary fractional excretion of electrolytes may serve as biomarkers for regulatory control for the nefarious administration of MgSO₄.  相似文献   

New protocol to separate llama sperm without enzymatic treatment using Androcoll-E™     

Mariana L. Bertuzzi  Fernanda G. Fumuso  Susana M. Giuliano  Marcelo H. Miragaya  María F. Gallelli  María I. Carretero 《Reproduction in domestic animals》2020,55(9):1154-1162

The objective of this study was to design a protocol to separate spermatozoa from seminal plasma of raw llama semen without prior enzymatic treatment using a single-layer centrifugation with Androcoll-E^™ (AE). Two experiments were performed: (a) samples were divided into three aliquots (1 ml) that were deposited on the top of 4, 5 or 6 ml of AE and were centrifuged at 800g for 20 min and (b) samples were divided into two aliquots (1 ml) that were deposited on the top of 4 ml of AE and were centrifuged at 600g or 1,000g for 20 min. Columns of 5 and 6 ml of AE showed a total sperm motility (TM) significantly lower, while in the 4 ml column, this parameter was not different from the TM of samples before the AE treatment. The percentage of spermatozoa with intact and functional membranes, normal morphology and intact acrosomes, as well as the percentages of sperm with highly condensed chromatin, was conserved (p ˃ .05) in the three column heights and in the two centrifugation speeds evaluated. In conclusion, the different column heights of AE (4, 5 and 6 ml) and the different centrifugation speeds used (600, 800 and 1,000g) allow separating spermatozoa of raw llama semen without enzymatic treatment, preserving the evaluated sperm characteristics. However, of all the studied treatments, centrifugation in the 4 ml column of AE at 800g would be the method of choice to process raw llama semen samples destined for reproductive biotechnologies.  相似文献   

Disorders of Calcium and Phosphate Metabolism in Horses     

Ramiro E. Toribio 《Veterinary Clinics of North America: Equine Practice》2011,27(1):129-147

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Preface: Endocrine Diseases     

Ramiro E. Toribio 《Veterinary Clinics of North America: Equine Practice》2011,27(1):xi

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