Isolation of Feline Herpesvirus 1 from the Trigeminal Ganglia of Acutely and Chronically Infected Cats     

Mark P. Nasisse DVM    Barbara J. Davis VMD    James S. Guy DVM  PhD    Michael G. Davidson DVM  Wendy Sussman BS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1992,6(2):102-103

Feline herpesvirus 1 (FHV-1) is one of the most common viral infections of domestic cats worldwide, estimated to cause 50% of all respiratory infections in this species. Feline herpesvirus 1 is also an important ocular pathogen of cats, causing conjunctivitis, epithelial and stromal keratitis, symblepharon formation, keratocon-junctivitis sicca, and corneal sequestration. Despite the importance of this viral disease, major questions remain unanswered concerning the pathogenesis of its most important manifestation, the recrudescent infection. Although the taxonomic classification of FHV-1 as an alpha herpesvirus implies the ability of FHV-1 to establish neural latency, attempts at recovering the virus from the trigeminal ganglia of latently infected cats have typically yielded negative results. This failure has stimulated speculation that neural tissue is not an important site for latent FHV-1. However, in the most successful of such studies, FHV-1 was isolated from the trigeminal ganglia of 3 of 17 cats using an explant technique. In the present study, we describe the successful isolation of FHV-1 from the trigeminal ganglia of cats using a similar tissue culture method.  相似文献   

Effects of atracurium on intraocular pressure, eye position, and blood pressure in eucapnic and hypocapnic isoflurane-anesthetized dogs   总被引：4，自引：0，他引：4  

McMurphy RM  Davidson HJ  Hodgson DS 《American journal of veterinary research》2004,65(2):179-182

OBJECTIVE: To determine effects of atracurium on intraocular pressure (IOP), eye position, and arterial blood pressure in eucapnic and hypocapnic dogs anesthetized with isoflurane. ANIMALS: 16 dogs. PROCEDURE: Ventilation during anesthesia was controlled to maintain Paco2 at 38 to 44 mm Hg in group- I dogs (n = 8) and 26 to 32 mm Hg in group-II dogs (8). Baseline measurements for IOP, systolic, diastolic, and mean arterial blood pressure, central venous pressure (CVP), and heart rate (HR) were recorded. Responses to peroneal nerve stimulation were monitored by use of a force-displacement transducer. Atracurium (0.2 mg/kg) was administered i.v. and measurements were repeated at 1, 2, 3, and 5 minutes and at 5-minute intervals thereafter for 60 minutes. RESULTS: Atracurium did not affect IOP, HR, or CVP Group II had higher CVP than group I, but IOP was not different. There was no immediate effect of atracurium on arterial blood pressure. Arterial blood pressure increased gradually over time in both groups. Thirty seconds after administration of atracurium, the eye rotated from a ventromedial position to a central position and remained centrally positioned until 100% recovery of a train-of-four twitch response. The time to 100% recovery was 53.1 +/- 5.3 minutes for group I and 46.3 +/- 9.2 minutes for group II. CONCLUSIONS AND CLINICAL RELEVANCE: Atracurium did not affect IOP or arterial blood pressure in isoflurane-anesthetized dogs. Hyperventilation did not affect IOP or the duration of effect of atracurium.  相似文献   

Urodynamic effects of a percutaneously controlled static hydraulic urethral sphincter in canine cadavers     

Adin CA  Farese JP  Cross AR  Provitola MK  Davidson JS  Jankunas H 《American journal of veterinary research》2004,65(3):283-288

OBJECTIVE: To describe a percutaneously controlled static hydraulic urethral sphincter (SHUS) and evaluate urodynamic effects of the SHUS in canine cadavers. SAMPLE POPULATION: Cadavers of 6 adult female dogs. PROCEDURE: Cadavers were obtained immediately after dogs were euthanatized. Baseline maximal urethral closure pressure (MUCP) and cystourethral leak point pressure (CLPP) were measured by use of a urethral pressure profilometer. An SHUS system was constructed by use of a silicone vascular occluder and subcutaneous infusion port. The SHUS system was then placed around the pelvic urethra in each cadaver. Measurements of MUCP and CLPP were repeated after varying occlusion of the SHUS (0%, 25%, and 50% occlusion). Baseline MUCP and CLPP values were compared with values obtained at 0%, 25%, and 50% occlusion of the SHUS by use of repeated-measures ANOVA. RESULTS: Mean +/- SD MUCP for canine cadavers was 7 +/- 1.3 cm H2O at baseline, which increased to 127 +/- 53 cm H2O after 50% occlusion of the SHUS. Mean CLPP was 11 +/- 8.6 cm H2O at baseline, which increased to 73 +/- 38 cm H2O after 50% occlusion of the SHUS. Mean MUCP and CLPP were significantly associated with the amount of occlusion. CONCLUSIONS AND CLINICAL RELEVANCE: The SHUS had positive effects on MUCP and CLPP in canine cadavers. Therefore, additional evaluation of the SHUS in live dogs is warranted.  相似文献   

Sensitivities of three county health department surveillance systems for child-related dog bites: 261 cases (2000)   总被引：1，自引：0，他引：1  

Tan RL  Powell KE  Lindemer KM  Clay MM  Davidson SC 《Journal of the American Veterinary Medical Association》2004,225(11):1680-1683

OBJECTIVES: To determine incidence of child-related dog bites and sensitivities of 3 county health department dog-bite surveillance systems. DESIGN: Retrospective study. STUDY POPULATION: Child-related dog-bite data obtained from surveillance systems of 3 counties in Georgia in the year 2000. PROCEDURE: To characterize the sensitivity of health department dog-bite surveillance systems, 9 other potential sources of dog-bite records that matched records by victim name, age, gender, and incident date were evaluated. The number of reported bites and the most productive sources for identifying additional cases were determined. The Chandra Sekar-Deming capture-recapture method was used to estimate the number of unreported bites, and estimates of dog-bite incidence rates were made. RESULTS: 40, 36, and 185 dog bites were reported in the 3 counties, respectively. Capture-recapture calculations estimated an additional 9, 5, and 128 dog bites in these counties, respectively. Local health departments recorded 45.5% to 82.5% of dog bites. Local hospital emergency departments, police departments, and a rabies-testing laboratory received additional reports. Among these data sources, local hospital emergency department records were the best source for identifying additional cases. CONCLUSIONS AND CLINICAL RELEVANCE: Dog bites are a preventable cause of childhood injuries, and surveillance is a critical tool for tracking childhood dog bites in the community. Counties should use combined data from local health departments, local hospital emergency departments, and police departments to implement or revise dog-bite prevention programs.  相似文献   

Theriogenology question of the month. Acute peritonitis secondary to intra-abdominal semen deposition     

Slater LA  Davidson AP  Dahlinger J 《Journal of the American Veterinary Medical Association》2004,225(10):1535-1537

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Unpalatable opinions     

Chevis R 《Australian veterinary journal》2004,82(6):339

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Verification of sensitivity and specificity of group a rotavirus detection in piglets faeces with monoclonal blocking ELISA methods   总被引：1，自引：0，他引：1  

Rodák L  Smíd B  Nevoránková Z  Smítalová R  Valícek L 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2004,51(4):160-165

Monoclonal antibodies to group A rotavirus Vp6 protein were prepared and used for verification of three blocking enzyme-linked immunosorbent assay (ELISA) modifications to detect rotavirus A. Selected competitive blocking ELISA (CB-ELISA) and electron microscopy (EM) were used for examination of 194 field faecal samples of piglets affected with diarrhoea. Rotavirus was detected in 43 samples (22.2%) by CB-ELISA method, whereas in 26 (13.4%) samples by EM examination. However, of 26 samples positive by EM, rotavirus A was detected by CB-ELISA in 19 (73.1%) samples; indicating the share of group A rotavirus in all cases of gastroenteritis caused by rotavirus. The sensitivity and specificity of the CB-ELISA was verified both by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhoea virus (PEDV) in each analysis and by comparative examination of samples with the commercial ELISA kit. The CB-ELISA sensitivity was positively affected by examination of samples in the presence of chelating agent.  相似文献   

Characterization of flavonol conjugates in immature leaves of pak choi [Brassica rapa L. Ssp. chinensis L. (Hanelt.)] by HPLC-DAD and LC-MS/MS     

Rochfort SJ  Imsic M  Jones R  Trenerry VC  Tomkins B 《Journal of agricultural and food chemistry》2006,54(13):4855-4860

The flavonoid composition of immature leaves of pak choi [Brassica rapa L. ssp. chinensis L. (Hanelt.)] was investigated. Flavonol aglycone content was measured in 11 pak choi varieties, indicating significant differences (P < 0.05) in content between varieties and relatively high contents of kaempferol and isorhamnetin. Levels of quercetin ranged from 3.2 to 6.1 mg/100 g of dry weight (DW), whereas levels of isorhamnetin and kaempferol were significantly higher (8.1-35.1 and 36.0-102.6 mg/100 g of DW, respectively). A large number of glycoside and hydroxycinnamic acid derivatives of quercetin, kaempferol, and isorhamnetin were identified in cv. 'Shanghai' by LC/UV-DAD/ESI-MS/MS. The UV-DAD data allowed identification of hydroxycinnamic acid derivatives, but detailed MS/MS fragmentations were required for the structure elucidation. Pak choi could be a potentially important source of dietary flavonols, in particular, kaempferol and isorhamnetin.  相似文献   

The transcriptome of the sea urchin embryo     

Samanta MP  Tongprasit W  Istrail S  Cameron RA  Tu Q  Davidson EH  Stolc V 《Science (New York, N.Y.)》2006,314(5801):960-962

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Phosphatized polar lobe-forming embryos from the Precambrian of southwest China     

Chen JY  Bottjer DJ  Davidson EH  Dornbos SQ  Gao X  Yang YH  Li CW  Li G  Wang XQ  Xian DC  Wu HJ  Hwu YK  Tafforeau P 《Science (New York, N.Y.)》2006,312(5780):1644-1646

In developing embryos of some extant spiralian animals, polar lobe formation is one of the symmetry-breaking mechanisms for segregation of maternal cytoplasmic substances to certain blastomeres and not others. Polar lobe formation leads to unique early cleavage morphologies that include trilobed, J-shaped, and five-lobed structures. Fossil embryos similar to modern lobeforming embryos are recognized from the Precambrian Doushantuo Formation phosphates, Weng'an, Guizhou Province, China. These embryos are abundant and form a developmental sequence comparable to different developing stages observed in lobe-forming embryos of extant spiralians. These data imply that lobe formation is an evolutionarily ancient process of embryonic specification.  相似文献