Effect of aging on lees and of three different dry yeast derivative products on Verdejo white wine composition and sensorial characteristics     

Del Barrio-Galán R  Pérez-Magariño S  Ortega-Heras M  Williams P  Doco T 《Journal of agricultural and food chemistry》2011,59(23):12433-12442

A study was made of the effect of aging on lees and of three different commercial yeast derivative products of different composition and degree of purification on the phenolic compounds, color, proteins, polysaccharides, and sensorial characteristics of white wines. The results obtained showed that the lees and yeast derivative products can interact or adsorb some of the phenolic compounds present in wines, reducing their concentration. This reduction depends on the treatment applied, the phenolic compound analyzed, and the stage of vinification or aging process. The use of lees and yeast derivative products can reduce the color intensity and the browning of the wines immediately following treatment. The monosaccharide and polysaccharide content of yeast derivative products depends on the manufacturing process and degree of purification of the product, both of which have an influence on wine treatments. After 6 months in the bottle, both the aging on lees and the treatment with commercial yeast derivative products gave rise to wines with better sensorial characteristics than in the case of the control wines.  相似文献   

Phenolic composition in grape (Vitis vinifera L. cv. Malbec) ripened with different solar UV-B radiation levels by capillary zone electrophoresis     

Berli F  D'Angelo J  Cavagnaro B  Bottini R  Wuilloud R  Silva MF 《Journal of agricultural and food chemistry》2008,56(9):2892-2898

The responses of Vitis vinifera L. cv. Malbec to different solar ultraviolet-B radiation (UV-B) levels were assessed in two contrasting situations, under sunlight with full UV-B (+UV-B) and filtered UV-B (-UV-B), in three different locations at 500, 1000, and 1500 m above sea level (asl). To evaluate the effects of radiation, a simple, accurate, and rapid method for the separation and simultaneous determination of representative phenolic compounds in grape berry skins by capillary zone electrophoresis was developed. Separation was carried out in less than 20 min with 20 mM sodium tetraborate buffer containing 30% methanol, pH 9.00. The procedure is fast and reliable, and extracted grape berry skins can be directly analyzed without prior sample cleanup procedure. Berry skins from the +UV-B treatment at 1500 m asl showed the highest levels of total polyphenols anthocyanins, and resveratrol, compared with the -UV-B treatment at this altitude.  相似文献   

Evidence of Migration and Endophytic Presence of Agrobacterium tumefaciens in Rose Plants   总被引：2，自引：0，他引：2  

Rubén Martí  Jaime Cubero  Antonio Daza  Jaime Piquer  Carmen I. Salcedo  Clara Morente  María M. López 《European journal of plant pathology / European Foundation for Plant Pathology》1999,105(1):39-50

Agrobacterium tumefaciens was isolated from stem tumors of several rose cultivars showing that the bacterium is the causal agent of aerial galls in rose plants. No differences were observed in the characteristics of the Agrobacterium isolates from crown or aerial galls. Stem inoculation of ten rose cultivars showed that all of them were susceptible to A. tumefaciens but differences in the size of the resulting tumors were observed. The movement of A. tumefaciens in rose plants was demonstrated using two wild type strains and two antibiotic resistant mutants. Three months after inoculation, the inoculated strains were recovered in the roots, crown and below and above the inoculation site but low numbers of pathogenic Agrobacterium cells were isolated. New tumors appeared in 5% of the noninoculated wounds. A. tumefaciens was isolated from the stem at different distances from the tumor in naturally infected plants. In symptomless commercial plants, the isolation from the roots, crown and at different stem levels demonstrated the existence of systemic and latent infections in rose. Direct isolation using a nonselective and selective media with or without a previous enrichment step were efficient methods for isolating tumorigenic Agrobacterium from the different parts of rose plants.  相似文献   

Local terrestrial snails as natural intermediate hosts of the zoonotic parasite Angiostrongylus cantonensis in the new European endemic area of Valencia,Spain     

Màrius V. Fuentes  Mercedes Gomez-Samblas  Orly Richter  Sandra Sáez-Durán  Rubén Bueno-Marí  Antonio Osuna  María Teresa Galán-Puchades 《Zoonoses and public health》2024,71(4):451-456

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Estimation of stand-level aboveground biomass dynamics using tree ring analysis in a Chinese fir plantation in Shitai County,Anhui Province,China     

Xiaolu Tang  Yuanchang Lu  Lutz Fehrmann  David I. Forrester  Rubén Guisasola-Rodríguez  César Pérez-Cruzado  Christoph Kleinn 《New Forests》2016,47(2):319-332

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Effect of additives on subcritical water hydrolysis of whey protein isolate     

Espinoza AD  Morawicki RO 《Journal of agricultural and food chemistry》2012,60(20):5250-5256

The objective was to examine the effect of the additives acetic acid, lactic acid, sodium bicarbonate, sodium chloride, and sodium hydroxide on the hydrolysis of whey protein isolate with subcritical water. A screening experimental design was used to study the effect of temperature, time, and additives. The most influential additive, sodium bicarbonate, along with temperature and time was used in a second experimental design to predict the treatment conditions to maximize the degree of hydrolysis and production of free amino acids. The maximum degree of hydrolysis achieved was 50% at a concentration of 1.24 M sodium bicarbonate, 291 °C, and 28 min. The highest concentration of total amino acids was 83.0 mg/g of whey protein isolate with 0.83 M sodium bicarbonate at 264 °C for 29 min. Compared to water alone, sodium bicarbonate increased the degree of hydrolysis 4-fold and the production of amino acids by 44% and decreased peptides' molecular weight.  相似文献   

Effect of acetylsalicylic acid on soft rot produced byErwinia carotovora subsp.carotovora in potato tubers under greenhouse conditions     

María M. López  María José López-López  Rubén Martí  Javier Zamora  Jesús López-Sanchez  Ramona Beltra 《Potato Research》2001,44(2):197-206

Summary The efficiency of acetylsalicylic acid (ASA) in inducing localised acquired resistance against infection byErwinia carotovora subsp.carotovora was evaluated by treating potato tubers with ASA at three concentrations. Three days after treatments, tubers were inoculated withE. carotovora subsp.carotovora by wound inoculation or by irrigation with a bacterial suspension. Experiments were performed for two consecutive years. Statistical analysis revealed that treatment of tubers by immersion in ASA solutions at low concentrations induced a significant reduction in the soft rot incidence. Wounding of the tubers was the most effective inoculation method and ASA at concentration of 0.0125% (w/v), pH 7 was more efficient than at 0.025 or 0.05%. No phytotoxicity of such treatment was observed.  相似文献   

Land-use changes alter CO2 flux patterns of a tall-grass Andropogon field and a savanna-woodland continuum in the Orinoco lowlands     

San José J  Montes R  Grace J  Nikonova N 《Tree physiology》2008,28(3):437-450

Land use changes in the savannas of the Orinoco lowlands have resulted in a mosaic of vegetation. To elucidate how these changes have affected carbon exchanges with the atmosphere, we measured CO2 fluxes by eddy covariance and soil CO2 efflux systems along a disturbance gradient beginning with a cultivated tall-grass Andropogon field (S1) and extending over three savanna sites with increasing woody cover growing above native herbaceous vegetation. The savanna sites included a herbaceous savanna (S2), a tree savanna (S3) and a woodland savanna (S4). During the wet season, maximum diurnal net ecosystem exchange (NEE) over the S1-S4 sites was 6.6-9.3, 6.6-7.9, 10.6-11.3 and 9.3-10.6 micromol m(-2) s(-1), respectively. The rate of CO2 uptake over S1 was lower than that for C4 grasses elsewhere because of pasture degradation. Soil respiration and temperature were exponentially related when soil water content (theta) was above 0.083 m(3) m(-3); however, soil respiration declined markedly as theta decreased from 0.083-0.090 to 0.033-0.056 m(3) m(-3). There were bursts of CO2 emission when dry soils were rewetted by rainfall. During the wet season, all sites constituted carbon sinks with maximum net daily ecosystem production (NEP) of 2.1, 1.7, 2.1 and 2.1 g C m(-2) day(-1), respectively. During the dry season, the savanna sites (S2-S4) became carbon sources with maximum emission fluxes of -0.5, -1.4 and -1.6 g C m(-2) day(-1), respectively, whereas the tall-grass field (S1) remained a carbon sink with a maximum NEP of 0.3 g C m(-2) day(-1) at the end of the season. For all measurement periods, annual NEP of sites S1-S4 was 366, 6, 116 and 139 g C m(-2), respectively. Comparisons of carbon source/sink dynamics across a wide range of savannas indicate that savanna carbon budgets can change in sign and magnitude. On an annual basis, gross primary production over the S1-S4 stands was 797, 803, 136 and 1230 g C m(-2), respectively. Net primary productivity (NPP) of the S1-S4 stands, calculated from eddy covariance measurements as the daily sum of NEE and day and night heterotrophic respiration was 498, 169, 181 and 402 g C m-2 year-1, respectively. These values were slightly higher than NPP based on harvest measurements (432, 162, 176 and 386 g C m(-2) year(-1), respectively), presumably because fine roots were incompletely harvested. Soil water content limited carbon uptake at all sites, and water-use efficiency (WUE) was related to rainfall dynamics. During the dry season, all sites except the cultivated tall-grass Andropogon field (S1) had a negative WUE. Although our results are specific to the Orinoco vegetational mosaic, the effects of land-use practices on the controls and physiological functions of the studied ecosystems may be generalized to other savannas.  相似文献   

Chemistry. Targeting specific C-H bonds for oxidation     

Mas-Ballesté R  Que L 《Science (New York, N.Y.)》2006,312(5782):1885-1886

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A longitudinal study of the diversity and dynamics of Mycoplasma hyopneumoniae infections in pig herds     

Vranckx K  Maes D  Sacristán Rdel P  Pasmans F  Haesebrouck F 《Veterinary microbiology》2012,156(3-4):315-321

A longitudinal study was carried out to investigate the diversity and persistence of Mycoplasma hyopneumoniae (M. hyopneumoniae) strains in four infected pig herds. In each herd, 20 pigs were randomly selected and blood and/or bronchoalveolar lavage (BAL) fluid was collected at 6, 10, 14 and 26 weeks of age. In the BAL fluid, quantitative PCR and MLVA (multiple-locus variable number of tandem repeats (VNTR) analysis) testing were performed for detection and typing of M. hyopneumoniae strains, respectively. At 26 weeks of age, the prevalence and severity of lung lesions were recorded at slaughter (minimum 50 pigs belonging to the same batch as the investigated pigs). The percentage of pigs testing positive on qPCR increased from 35% at 6 weeks to 96% at 26 weeks of age. With MLVA testing, positive pigs were found from 14 weeks onwards. Within each herd, only one distinct strain was detected, although clonal variants were identified in two herds. In three of the herds, the strain remained present until slaughter age. The percentage of pigs with Mycoplasma-like lesions ranged from 38% to 98%, and the average pneumonia score ranged from 1.7 to 11.9, respectively. The present field study documented that within a herd, mainly one distinct M. hyopneumoniae strain was present that persisted in the same animals for at least 12 weeks. This implies that the immune response of the animals following infection is not able to rapidly clear the infection from the respiratory tract.  相似文献