Genetic analysis of medfly populations in an area of sterile insect technique applications     

Sancho  Rubén  Guillem-Amat  Ana  López-Errasquín  Elena  Sánchez  Lucas  Ortego  Félix  Hernández-Crespo  Pedro 《Journal of pest science》2021,94(4):1277-1290

Journal of Pest Science - The sterile insect technique (SIT) is widely used in integrated pest management programs for the control of the Mediterranean fruit fly (medfly), Ceratitis capitata. The...  相似文献   

Inheritance of shelf life and other quality traits of tomato fruit estimated from F<Subscript>1</Subscript>’s,F<Subscript>2</Subscript>’s and backcross generations derived from standard cultivar, <Emphasis Type="Italic">nor</Emphasis> homozygote and wild cherry tomato     

Gustavo Rubén Rodríguez  Guillermo Raúl Pratta  David Rodolfo Liberatti  Roxana Zorzoli  Liliana Amelia Picardi 《Euphytica》2010,176(1):137-147

A tomato cultivar with high quality fruit and a long shelf life is a main goal in tomato breeding and it would be achieved using wild germplasm. The objective of this work was to explore the inheritance for fruit quality traits, especially fruit shelf life, in three tomato crosses using a standard Argentinean cultivar (Ca, cv ‘Caimanta’), a ripening mutant (nor, homozygous for the nor gene) of Solanum lycopersicum, and a wild cherry type (Ce, LA1385 of S. lycopersicum var. cerasiforme). The wild parent had a shorter fruit shelf life than the mutant genotype but higher than Ca. When the Ce genotype was analyzed in hybrid combination, the F₁ (Ca×Ce) was similar to the wild genotype for shelf life whereas the F₁ (nor × Ce) had a longer shelf life. Both F₁ crosses and backcrosses to the cherry type genotype had significantly lower fruit weight than the cultivated genotypes but higher than the cherry type parent. In the F₂ analysis, it was found that the inheritance underlying quality traits is complex since non allelic interactions were detected. A significant additive genetic variance was found for fruit shelf life as well as for other fruit quality traits in each cross. The genetic parameters analyzed by mean values and variances in parental, F₁ and F₂ and backcross generations indicated that the cross between the normal ripening cultivar and LA1385 of S. lycopersicum var. cerasiforme offers the best possibility to obtain long shelf life tomato genotypes with good fruit quality.  相似文献   

Changes on the Phytoavailability of Nutrients in a Mine Soil Reclaimed with Compost and Biochar     

Alfonso Rodríguez-Vila  Rubén Forján  Rafael S. Guedes  Emma F. Covelo 《Water, air, and soil pollution》2016,227(12):453

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Virtual Screening of Plant Volatile Compounds Reveals a High Affinity of Hylamorpha elegans (Coleoptera: Scarabaeidae) Odorant-Binding Proteins for Sesquiterpenes From Its Native Host     

Angélica González-González  Rubén Palma-Millanao  Osvaldo Yá?ez  Maximiliano Rojas  Ana Mutis  Herbert Venthur  Andrés Quiroz  Claudio C. Ramírez 《Journal of insect science (Online)》2016,16(1)

Hylamorpha elegans (Burmeister) is a native Chilean scarab beetle considered to be a relevant agricultural pest to pasture and cereal and small fruit crops. Because of their cryptic habits, control with conventional methods is difficult; therefore, alternative and environmentally friendly control strategies are highly desirable. The study of proteins that participate in the recognition of odorants, such as odorant-binding proteins (OBPs), offers interesting opportunities to identify new compounds with the potential to modify pest behavior and computational screening of compounds, which is commonly used in drug discovery, may help to accelerate the discovery of new semiochemicals. Here, we report the discovery of four OBPs in H. elegans as well as six new volatiles released by its native host Nothofagus obliqua (Mirbel). Molecular docking performed between OBPs and new and previously reported volatiles from N. obliqua revealed the best binding energy values for sesquiterpenic compounds. Despite remarkable divergence at the amino acid level, three of the four OBPs evaluated exhibited the best interaction energy for the same ligands. Molecular dynamics investigation reinforced the importance of sesquiterpenes, showing that hydrophobic residues of the OBPs interacted most frequently with the tested ligands, and binding free energy calculations demonstrated van der Waals and hydrophobic interactions to be the most important. Altogether, the results suggest that sesquiterpenes are interesting candidates for in vitro and in vivo assays to assess their potential application in pest management strategies.  相似文献   

Effect of diet and rearing conditions on growth and survival throughout developmental stages of larvae and juveniles of dusky grouper <Emphasis Type="Italic">Epinephelus marginatus</Emphasis> (Lowe 1834)     

Cabaleiro Santiago  Lucía Barreiro  Rubén Caamaño 《Aquaculture International》2018,26(4):1109-1118

Two rearing conditions, tank light-dimensions and diet, were tested in dusky grouper—larvae and juveniles—in order to improve larval rearing techniques for this species. Larvae reared in high-volume and fluorescent tubes showed the highest survival rate at 18 dph (17.67%, 16%). This might be due to a higher depth and less trapping area during surface death in high-volume tanks than in low volume. Besides, a 500-lx evenly distributed light with fluorescent tubes might improve survival rate at early stages rather than illumination with energy-efficient light bulbs (4.68%, 9.64%). Light bulbs created shaded areas with less illumination inducing low larval activity. First feeding was performed with minimum rotifer lorica width of 102?±?10.2 μm. No mixed diet was supplied. Throughout metamorphosis, stress shock syndrome appeared to be the main cause of mortality. Diet A showed best growth and survival (4.1–7.36%) during this period probably due to its high HUFA content (9.5%). Contrary to what was observed during first stages, groupers showed best performance with lower illumination. Cannibalism was observed during this period in all culture conditions.  相似文献   

Heifers immunized with whole-cell and membrane vaccines against Tritrichomonas foetus and naturally challenged with an infected bull     

Cobo ER  Cano D  Rossetti O  Campero CM 《Veterinary parasitology》2002,109(3-4):169-184

The performance of a whole-cell vaccine and the other vaccine with cellular membranes of Tritrichomonas foetus applied to heifers naturally challenged by mating with an infected bull was determined. Forty heifers were divided into three groups: a control group (n=16) without immunizing, another group (n=12) immunized with whole cells (10(8)/dose) and a third group (n=12) immunized with cellular membranes (300 micro g of membranes/dose protein). The females were subcutaneously vaccinated at 3-week on two occasions and received a third intravaginal booster dose. After 3 weeks of the last vaccinal doses, the heifers were served by a T. foetus infected bull over 90-day period. The mean duration of infection for membrane-vaccinated heifers was 60 days +/-25, compared with 63 days +/-35.8 of infection for whole-cell-vaccinated heifers and 79 days +/-41.3 for control heifers. Calving rates were 6/12 for membrane-vaccinated heifers, 3/12 for whole-cell-vaccinated animals, and 2/16 for control animals. Fetal mortality rates were 3/12 for membrane-vaccinated animals, 4/12 for those vaccinated with whole cells and 10/16 for control animals. These reproductive parameters were significantly different (P<0.05) between heifers vaccinated with membranes and control heifers. The hemolytic test and enzyme-linked immunoabsorbent assay (ELISA) with T. foetus antigen showed that serum immunoglobulins peaked before and during the breeding period. The heifers vaccinated with membranes developed an important response during the critical period of fetal loss, second and third month of the breeding time, and another month after the same period. The ELISA method was more sensitive and more reliable than the hemolytic test for the evaluation of the systemic immune response in females infected and/or vaccinated with T. foetus.  相似文献   

Ophthalmic patterns of captive brown brocket deer (Mazama gouazoubira)     

Bianca C Martins  Arianne P Oriá  Ana L G Souza  Carla F Campos  Denise E Almeida  Roberta A Duarte  Christiane P Soares  José A S Zuanon  Carlos B Neto  José M B Duarte  Rubén P Schocken-Iturrino  José L Laus 《Journal of zoo and wildlife medicine》2007,38(4):526-532

Captive brown brocket deer (Mazama gouazoubira) were manually restrained to assess tear production by the Schirmer tear test I to measure intraocular pressure by applanation tonometry, to examine ocular conjunctival epithelial cells via cytologic and histologic samples, and to survey ocular conjunctival microflora by microbiologic culture. The mean value for the Schirmer tear test I was 8.9 +/- 1.8 mm/min, and the mean intraocular pressure was 15.3 +/- 3.1 mm Hg. Conjunctival epithelium contained stratified pavimentous layers of cells, and the microflora consisted of predominantly gram-positive bacteria.  相似文献   

Molecular performance of Prl and Gh/Igf1 axis in the Mediterranean meager, <Emphasis Type="Italic">Argyrosomus regius</Emphasis>, acclimated to different rearing salinities     

Khaled Mohammed-Geba  Antonio Astola González  Rubén Ayala Suárez  Asmaa Galal-Khallaf  Juan Antonio Martos-Sitcha  Hany Mohammed Ibrahim  Gonzalo Martínez-Rodríguez  Juan Miguel Mancera 《Fish physiology and biochemistry》2017,43(1):203-216

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Screening of Argentinian plants for pesticide activity     

Palacios SM  Maggi ME  Bazán CM  Carpinella MC  Turco M  Muñoz A  Alonso RA  Nuñez C  Cantero JJ  Defago MT  Ferrayoli CG  Valladares GR 《Fitoterapia》2007,78(7-8):580-584

Aerial parts of 27 plant species native to Argentina were tested in anti-insect, germination inhibition and bactericide bio-assays. In antifeedant assays on Epilachna paenulata larvae, 11 species showed strong feeding deterrent effects (higher than 90% at 200 microg/cm(2)). Twelve plants strongly inhibited the germination of Avena sativa seeds, but only six inhibited Raphanus sativum germination at 10 mg/ml. Four plants showed complete growth inhibition of Escherichia coli at a concentration of 2 mg/ml.  相似文献   

Seasonal variations in the immunological and physiological parameters of the Pacific oyster Crassostrea gigas cultured in Bahía de Macapule (Sinaloa,Mexico)     

Antonio Luna‐González  María De Jesús Romero‐Geraldo  Ángel Campa‐Córdova  Javier Orduña‐Rojas  Rubén Valles‐Jiménez  César A Ruíz‐Verdugo 《Aquaculture Research》2008,39(14):1488-1497

Triploid Crassostrea gigas were cultured during 13 months in Nestier‐type oyster trays. The impact of environmental parameters on the physiological and immunological parameters was evaluated. Temperature, salinity and seston were recorded monthly. Seventeen oysters were sampled monthly for immunological and condition index (CI) analyses. Samples were obtained as a haemolymph lysate supernatant (HLS). Protein content was determined using the Bradford method. The activity of hydrolytic enzymes was determined using the API ZYM kit and the lysoplate assay. Seston showed different patterns throughout the cycle. Condition index showed a positive correlation with the protein content of HLS. Protein showed a negative correlation with temperature. Eleven hydrolytic enzymes were detected in samples and higher enzymatic activity corresponded to leucine arylamidase and esterase. Leucine arylamidase and lysozyme activity showed a positive correlation with temperature. Oyster mortality was 28% in our modules and 70% in the oyster farm. Oysters showed low values of CI and haemolymph protein content in summer–autumn when mortalities were observed in the culture system. This finding suggests that these stressed oysters may have insufficient energy to invest in their immune system. It appears that oyster mortality in the culture system resulted from a combination of animal overcrowding, high temperature and low salinity.  相似文献