Canine squamous cell carcinoma: Electrochemotherapy association with surgery and correlation with overall survival     

Rúbia Monteiro de Castro Cunha  Gleidice Eunice Lavalle  Fernanda Camargo Nunes  Ayisa Rodrigues de Oliveira  Renato de Lima Santos  Roberto Baracat de Araújo 《Veterinary and comparative oncology》2023,21(2):240-254

Squamous cell carcinoma (SCC) is an important malignancy in dogs, due to its incidence and clinical presentation, which can be of locally aggressive single or multiple lesions with a metastatic potential. The objective of this investigation was to evaluate SCC response to treatment, anatomopathological and immunohistochemical characteristics, disease-free interval and overall survival time. 54 dogs with histopathologically diagnosed SCC were included in this study. Their mean age was 9.16 years with a range of 1–14 years. Of the 54 animals in the study, 34 (65.4%) had white skin and white fur coats. There was a significant correlation between fur coat colour and the development of tumours in areas of sun exposure (p = .001). Animals with tumours in areas of the body exposed to the sun had longer overall survival time than animals with tumours in areas not associated with sun exposure (p = .001). Surgery combined with electrochemotherapy (ECT) yielded a survival rate 32% higher than using a surgical approach alone (HR = 0.32, p = .038, IC = 0.11–0.94). ECT, with or without surgery, had an objective response rate of 90.9%. Local lymph node and/or distant site metastasis at diagnosis, or at some point during follow-up, occurred in 34.6% (18/52) of animals. Animals with tumours in sun exposed locations had more aggressive histopathological characteristics but had longer overall survival time. This is probably due to individualised therapeutic treatment with both surgery and ECT.  相似文献   

Outbreak of shoot blight and dieback of Eucalyptus spp., caused by Pseudoplagiostoma eucalypti in Brazil     

Edival Ângelo Valverde Zauza  Lúcio Mauro da Silva Guimarães  Nilza de Lima Pereira Sales  Samuel Alves dos Santos  Rafael Ferreira Alfenas  Acelino Couto Alfenas 《Forest Pathology》2023,53(2):e12806

An outbreak of a new and severe disease was observed in Eucalyptus plantations of Bahia state, Brazil. An Ascomycota fungus has been frequently associated with the main symptoms of the disease namely leaf spot, branch cankers, shoot blight, defoliation, and dieback. Based on morphological characteristics, phylogenetic analysis (ITS and TEF-1α genes), and pathogenicity test on Eucalyptus plants, Pseudoplagiostoma eucalypti was identified as the causal agent of the disease. Although P. eucalytpi has been known from in Brazil since 1998, this is the first report of it causing severe disease and die-back on Eucalyptus spp. and we also record new symptoms associated with the pathogen.  相似文献   

Replacing Wheat Flour with Debittered and Fermented Lupin: Effects on Bread’s Physical and Nutritional Features     

Villacrés  Elena  Cueva  Paúl  Díaz  Milene  Rosell  Cristina M. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):569-575

Plant Foods for Human Nutrition - In this study the breadmaking potential of lupin flour from L. mutabilis after being debittered (DLF) and solid state fermented (FLF) was evaluated in lupin-wheat...  相似文献   

Marine Anticancer Agents: An Overview with a Particular Focus on Their Chemical Classes     

Marilia Barreca  Virginia Span  Alessandra Montalbano  Mercedes Cueto  Ana R. Díaz Marrero  Irem Deniz  Ayegül Erdoan  Lada Luki&#x; Bilela  Corentin Moulin  Elisabeth Taffin-de-Givenchy  Filippo Spriano  Giuseppe Perale  Mohamed Mehiri  Ana Rotter  Olivier P. Thomas  Paola Barraja  Susana P. Gaudêncio  Francesco Bertoni 《Marine drugs》2020,18(12)

The marine environment is a rich source of biologically active molecules for the treatment of human diseases, especially cancer. The adaptation to unique environmental conditions led marine organisms to evolve different pathways than their terrestrial counterparts, thus producing unique chemicals with a broad diversity and complexity. So far, more than 36,000 compounds have been isolated from marine micro- and macro-organisms including but not limited to fungi, bacteria, microalgae, macroalgae, sponges, corals, mollusks and tunicates, with hundreds of new marine natural products (MNPs) being discovered every year. Marine-based pharmaceuticals have started to impact modern pharmacology and different anti-cancer drugs derived from marine compounds have been approved for clinical use, such as: cytarabine, vidarabine, nelarabine (prodrug of ara-G), fludarabine phosphate (pro-drug of ara-A), trabectedin, eribulin mesylate, brentuximab vedotin, polatuzumab vedotin, enfortumab vedotin, belantamab mafodotin, plitidepsin, and lurbinectedin. This review focuses on the bioactive molecules derived from the marine environment with anticancer activity, discussing their families, origin, structural features and therapeutic use.  相似文献   

Management of yellow dwarf disease in Europe in a post‐neonicotinoid agriculture     

Louise Mc Namara  Kevin Gauthier  Lael Walsh  Gaël Thbaud  Michael Gaffney  Emmanuel Jacquot 《Pest management science》2020,76(7):2276-2285

Barley/cereal yellow dwarf viruses (YDVs) cause yellow dwarf disease (YDD), which is a continuous risk to cereals production worldwide. These viruses cause leaf yellowing and stunting, resulting in yield reductions of up to 80%. YDVs have been a consistent but low‐level problem in European cereal cultivation for the last three decades, mostly due to the availability of several effective insecticides (largely pyrethroids and more recently neonicotinoids) against aphid vectors. However, this has changed recently, with many insecticides being lost, culminating in a recent European Union (EU) regulation prohibiting outdoor use of the neonicotinoid‐insecticide compounds. This change is coupled with the growing challenge of insecticide‐resistant aphids, the lack of genetic resources against YDVs, and a knowledge deficit around the parameters responsible for the emergence and spread of YDD. This means that economic sustainability of cereal cultivation in several European countries including France and United Kingdom is now again threatened by this aphid‐vectored viral disease. In this review, we summarize the current knowledge on the YDV pathosystem, describe management options against YDD, analyse the impacts of the neonicotinoid ban in Europe, and consider future strategies to control YDV. © 2020 Society of Chemical Industry  相似文献   

Strategy to identify resistance to white mould associated with high yield for irrigated common bean in Brazil     

Renan C. Lima  Pablo H. Teixeira  Ari F. F. Souza  Trazilbo J. Paula Júnior  Hudson Teixeira  Miller S. Lehner  José E. S. Carneiro  Tiago S. Marçal  Rogerio F. Vieira 《Plant pathology》2020,69(6):1172-1184

Development of common bean cultivars with partial white mould resistance through breeding techniques has been a challenge in Brazil. As yet, lines/cultivars from breeding programmes have not been investigated for resistance; therefore, this study screened 107 lines/cultivars for their reactions to white mould in 14 preliminary trials conducted under irrigation. Thirteen resistant lines/cultivars (three of Andean origin) and six Mesoamerican cultivars (three intermediately resistant and three susceptible) were selected for further investigation. These lines/cultivars and the resistant control A195 were evaluated in six advanced trials and two straw tests to assess the effectiveness of the screening procedure. In 11 preliminary trials, screenings were performed under moderate/high or higher disease pressure. These pressures occurred in two advanced trials in which, when yields were averaged across moderate/high and high pressures, 10 Mesoamerican lines/cultivars selected for resistance yielded 14%, 23%, and 38% more than intermediately resistant cultivars, A195, and susceptible cultivars, with median disease ratings (1–9 scale) of 4.5, 5.7, 5.7, and 6.7, respectively. In the straw test, three Andean lines/cultivars (A195 included) and two susceptible cultivars in the field were among those with the highest levels of physiological resistance. Thus, field rating under high disease pressure and greenhouse rating did not correlate significantly, suggesting that field trials are critical to evaluating resistance and to identifying high-yielding beans. Therefore, lines/cultivars from breeding programmes assessed in field trials may provide a low cost and fast way to identify high-yielding bean cultivars with partial resistance to white mould in the subtropical southern hemisphere.  相似文献   

秸秆复合有机酿热物对大棚土壤环境的影响     

王娟  陈立新  邹春娇  萨日娜  刘力勇  杨龙  谭巍 《中国农学通报》2020,36(25):78-85

为解决黑龙江省早春大棚土壤温度低及设施土壤环境恶化问题,以‘千禧’番茄为材料,研究秸秆+牛粪(NGH)、秸秆+马粪(MGH)、秸秆+羊粪(YGH)、单一秸秆(JG),以未进行反应堆技术为对照(CK),对早春大棚‘千禧’番茄土壤温度、土壤理化性状、酶活性及微生物群落结构的影响。结果表明,与对照相比,各处理降低了土壤pH,提高了土壤温度、土壤EC、碱解氮、速效钾、有机质含量及土壤蔗糖酶、脲酶、磷酸酶、过氧化氢酶活性。经过秸秆生物反应堆处理的20 cm土层温度有明显的提升,其中秸秆+羊粪处理在反应启动后提升土壤温度效果最好;秸秆+羊粪(YGH)处理提高了土壤速效磷含量,是对照的2倍;秸秆+羊粪(YGH)处理土壤中4种酶活性均处于最高水平,分别比对照提高了91.9%、220%、18.5%、7.3%。各处理微生物多样性指数均显著高于对照,变化规律一致为秸秆+羊粪(YGH)>秸秆+牛粪(NGH)>秸秆+马粪(MGH)>秸秆(JG)>对照(CK);秸秆+羊粪(YGH)及秸秆+牛粪(NGH)处理对糖类、氨基酸类、酯类、醇类、胺类、酸类6类碳源的利用能力最强,显著高于其他处理和对照。应用主成分分析和聚类分析方法将大棚番茄土壤分为4个等级,秸秆+羊粪(YGH)处理为第一级,秸秆+牛粪(NGH)处理为第二级,秸秆+马粪(MGH)及单一秸秆(JG)处理为第三级,对照(CK)为第四级。应用秸秆复合有机酿热物生物反应堆促进了土壤环境的改善,以秸秆+羊粪(YGH)作为复合有机酿热物效果最佳。  相似文献   

基于双目立体视觉的重叠柑橘空间定位     

刘妤  刘洒  杨长辉  王恺  谢铌 《中国农业科技导报》2020,22(9):104-112

果实的精准识别和定位是智能采摘面临的难题之一。基于双目立体视觉,提出了一种针对户外重叠柑橘的三维空间定位方法。首先,从双目左右图像中提取重叠柑橘果实轮廓并进行高斯平滑,通过曲率分析,找出异常的轮廓像素点;其次,依次连接相邻两个异常像素点,分析该线段上的像素点到柑橘轮廓的距离,在相邻两正常线段的交点处完成重叠柑橘轮廓分割,并通过寻找异常线段剔除对应的非柑橘轮廓像素点;再者,采用最小二乘椭圆拟合方法重建柑橘目标轮廓,并获取柑橘的中心;最后,根据双目极线约束和图像相似度,对重叠柑橘中心点进行匹配,并基于视差原理计算柑橘中心的深度值及三维空间坐标,确定重叠柑橘的遮挡关系。户外实验结果表明,所提出的方法定位误差为6.38 mm,满足柑橘采摘机器人户外采摘作业的定位精度要求。  相似文献   

Spatial patterns of the Citrus leprosis virus and its associated mite vector in systems without intervention     

R. B. Bassanezi  A. B. C. Czermainski  F. F. Laranjeira  A. S. Moreira  P. J. Ribeiro Júnior  E. T. Krainski  L. Amorim 《Plant pathology》2019,68(1):85-93

Leprosis is caused by the Citrus leprosis virus cytoplasmic type and is vectored by the mite Brevipalpus yothersi. Miticide applications, which cost $54 million annually, are based on inspection for the presence of mites. The aim of the present study was to characterize the spatial patterns of B. yothersi-infested trees and trees with leprosis symptoms for further improvement in sampling and disease control. The presence of mites and the occurrence of leprosis were assessed over two years in 1160 Valencia trees and 720 Natal trees in a commercial sweet orange grove in Sao Paulo State, Brazil. To assess the natural growth and dispersal of mites and leprosis, mite populations were not controlled during the experimental period. Maps of mite-infested trees and trees with leprosis symptoms were analysed at three different levels of spatial hierarchy using complementary methods, i.e. among adjacent trees within and across rows, within quadrats, and the strength and orientation of aggregation among quadrats. The study showed that the spatial patterns of virus-infected and mite-infested trees were different, with a strong aggregation pattern of trees with leprosis symptoms that increased over time. Conversely, the spatial pattern of B. yothersi showed randomness or weak aggregation at all three spatial hierarchical levels. Disease incidence increased steadily in plots of both cultivars, unlike in mite-infested trees where incidence fluctuated over time. These results have important implications for the development of better management strategies for leprosis. Sampling methods and action thresholds for mite control should consider primary disease inoculum in addition to the incidence of mites.  相似文献   

基于ResNet-50深度卷积网络的果树病害智能诊断模型研究   总被引：2，自引：1，他引：1  

金瑛  叶飒  李洪磊 《农业图书情报学刊》2021,(4):58-67

[目的/意义]果树病害危及农业生产安全,运用人工智能技术帮助果农及时准确地识别果树病害对保障农业安全生产具有重要意义.[方法/过程]采用10 000张果树叶片病斑图像数据集,通过旋转、污化、增噪、切割等图像增强手段,提高样本图像的多样性;使用ResNet-50深度卷积网络模型,进行机器学习,获得果树病害识别模型,并基于...  相似文献