Effect of Prenatal and Neonatal Anti‐Androgen Flutamide Treatment on Aquaporin 5 Expression in the Adult Porcine Ovary          下载免费PDF全文

M Grzesiak  K Knapczyk‐Stwora  MR Luck  A Mobasheri  M Slomczynska 《Reproduction in domestic animals》2016,51(1):105-113

The growth of ovarian follicles is accompanied by fluid‐filled antrum formation. Water movement within the follicular wall is predominantly transcellular via membranous water channels named aquaporins (AQPs). Androgens are important regulators of mammalian folliculogenesis, and their prenatal and/or neonatal deficiency affects female fertility in adulthood. Therefore, this study was performed to determine whether gestational or neonatal exposure to the anti‐androgen flutamide influences androgen‐dependent AQP5 expression in pre‐antral and large antral follicles of adult pigs. Flutamide was injected into pregnant gilts between days 80 and 88 of gestation and into female piglets between days 2 and 10 post‐natally. The ovaries were collected from flutamide‐treated and non‐treated (control) sexually mature pigs. In pre‐antral follicles, AQP5 mRNA and protein levels were both downregulated following maternal (p < 0.01 and p < 0.01, respectively) and neonatal (p < 0.01 and p < 0.01, respectively) flutamide exposure. Likewise, the expression of mRNA (p < 0.01 and p < 0.001, respectively) and protein (p < 0.05 and p < 0.01, respectively) for AQP5 were diminished in large antral follicles in both groups. Immunohistochemistry showed decreased intensity of AQP5 immunoreaction in pre‐antral (p < 0.01) and large antral (p < 0.001) follicles following flutamide treatment. Moreover, radioimmunological analysis revealed that changes observed in AQP5 expression corresponded with diminished follicular androgens production after both maternal (p < 0.05 and p < 0.05, respectively) and neonatal (p < 0.05 and p < 0.01, respectively) flutamide administration. Therefore, AQP5 appears to be a potential regulator of follicular fluid accumulation, under androgen control, and may be a key factor in antral follicle growth.  相似文献   

Adjusted friction correction factors for center-pivots with an end-gun     

Sayed-Hossein Sadeghi  Troy Peters 《Irrigation Science》2013,31(3):351-358

A new method for calculating total friction head loss in center-pivots with an operational end-gun was developed. The proposed methodology is based on adjusting the previous friction correction factors for center-pivots with end-guns in order to correct their paradoxes and shortcomings. Equations presented in the current work are developed for center-pivots with a finite number of outlets along the lateral and constant outlet spacing and discharge as well as constant discharge and variable spacing. The proposed formulas depend on the number of outlets along the supply pipeline, the exponent of velocity term in the friction formula used and the distance that water is jetted by the end-gun. All equations reduce to the well-established equations for the friction correction factor when the end-gun is turned off. The equations presented here compare well to the stepwise friction calculation method, yet correct slight errors in the way that these friction correction factors were calculated in the past.  相似文献   

The effect of dietary coenzyme Q10 on plasma metabolites and hepatic gene expression in broiler breeder hens     

H. Sharideh  S. Zeinoaldini  M. Zaghari  M. Sadeghi 《British poultry science》2020,61(3):281-286

ABSTRACT

1. This study was performed to evaluate the effects of dietary supplementation of coenzyme Q10 (CoQ10) on laying rate, body weight, plasma metabolites and some liver gene expression in broiler breeder hens.

2. A total of 128 broiler breeder hens (Arbor Acres Plus, 47 weeks of age) were randomly distributed to four dietary groups supplemented with different levels of CoQ10 (0, 300, 600 or 900 mg/kg diet) with four replicates of eight hens each. During 47–54 weeks of age, laying rate, egg mass and body weight were recorded weekly. To assay plasma biochemical indicators, blood samples were collected at 54 weeks of age. At the end of the experiment, for evaluating the abdominal fat weight, liver weight and expression of the adiponectin and proliferator-activated receptor-α (PPAR-α) genes in the liver, eight hens per treatment were selected, weighed and humanely killed by decapitation.

3. Dietary supplementation of CoQ10 linearly decreased abdominal fat weight, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by increased levels of CoQ10. The plasma levels of glucose, cholesterol and alkaline phosphatase (ALP) activity were quadratically decreased by increased levels of CoQ10. The best plasma levels of glucose, cholesterol and ALP activity were estimated at 562.5, 633.3 and 517.8 mg CoQ10/kg diet, respectively. Adiponectin and PPARα gene expression exhibited a linear increased by increased levels of CoQ10.

4. In conclusion, addition of CoQ10 to the diet influenced lipid metabolism and expression of the adiponectin and PPAR-α genes, which might be partially due to the improvement in mitochondrial metabolism and energy production. However, further studies are necessary to determine the effects of CoQ10 on these indicators in broiler breeder hens during ageing.  相似文献   

Effect of Drought Stress and Different Levels of Nitrogen and Potassium Fertilizers on the Accumulation of Osmolytes and Chlorophyll in Rice (Oryza sativa L.)     

Rabiei  Zahra  Mohammadian Roshan  Naser  Sadeghi  Seyyed Mostafa  Amiri  Ebrahim  Doroudian  Hamid Reza 《Gesunde Pflanzen》2021,73(3):287-296

Gesunde Pflanzen - Management of plant nutrition using fertilizers is an approach to deal with drought and to increase water productivity in paddies. The aim of this study, therefore, was to...  相似文献   

Mitigating effects of slash on soil disturbance in ground-based skidding operations     

Berdimohammad Agherkakli  Seyed Hamidreza Sadeghi  Eric Zenner 《Scandinavian Journal of Forest Research》2014,29(5):499-505

Reinforcement of skid trails with slash has been shown to reduce soil disturbances, but there have not been any studies documenting the mitigating effects following traffic of harvest equipment traffic on steep mountainous skid trails. The objective of this study was to quantify potentially mitigating effects of slash cover on soil compaction and rutting on skid trails in mountainous hardwood forests. The effects of the placement of no slash (bare soil), light density slash (7.5 kg m^?2) and heavy density slash (17.5 kg m^?2) in a skid trail following one, five, and nine machine passes on both gentle slopes <20% and steep slopes >20% in a downhill skidding operation by a steel-tracked skidder were studied. Bulk density and rut depth increased following harvest equipment traffic on both slope gradients. Compared to bare soils, soil bulk density was not significantly reduced by light slash density; however, soil bulk density was significantly reduced by heavy slash up to five machine passes on steep slopes. Light and heavy slash significantly reduced rut depth in both slope classes. The study revealed a high protective role of slash, particularly on steep skid trails. However, benefits of slash to mitigate soil compaction were limited to five passes, after which the slash deteriorated and only provided benefits against rutting.  相似文献   

Survey of Australian equine veterinarians evaluating their biosecurity training and perceptions and opinions about the management of the 2007 equine influenza outbreak     

K Schemann  J‐ALML Toribio  MR Taylor  MP Ward  NK Dhand 《Australian veterinary journal》2014,92(4):93-100

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Optimization Protocol for Storage of Goldfish (Carassius auratus) Embryos in Chilled State     

F Shaluei  MR Imanpoor  A Shabani  MH Nasr‐Esfahani 《Reproduction in domestic animals》2014,49(2):210-218

A series of five experiments were conducted to explore suitable conditions for storing of goldfish embryos in a chilled state. The factors studied were embryo stage, storage temperature, physiological saline solutions and goldfish artificial coelomic fluid (GFACF) medium, antibiotics (penicillin and streptomycin), antioxidants (vitamin E, vitamin C), buffer (Hepes, Tris) and BSA (bovine serum albumin). First, goldfish embryos at eight developmental stages were incubated in aerated and dechlorinated tap water at 0°C for 24 h. Result shows that early developmental stages were most sensitive to chilling. Heartbeat‐stage goldfish embryos were chilled at 0, 4 or 8°C for up to 72 h in water, and chilled storage was possible only for up to 18, 24 and 48 h at 0, 4 and 8°C, respectively, without a decrease in viability. Chilling of goldfish embryos at 8°C in GFACF medium and Dettlaff's solution instead of water and other physiological saline solutions prolonged their viability (p < 0.01). Nevertheless, viability of chilled embryos in GFACF medium was slightly, but non‐significantly, higher than in Dettlaff's solution. Supplementation of the GFACF medium with antibiotics, Hepes or BSA increased the viability of chilled embryos, but the tested vitamin E analogue Trolox, vitamin C or Tris concentration had no effect on embryo viability. The outcome of this series of experiments shows that heartbeat‐stage goldfish embryos could be chilled for 60 h in GFACF supplemented with 25 mm Hepes, 100 U/ml penicillin, 10 μg/l streptomycin and 1 g/l BSA in such a way that embryonic development does not proceed, and viability is not lost.  相似文献   

Influence of Gonadotrophin‐Induced First Oestrus on Gilt Fertility     

DM Hidalgo  RM Friendship  L Greiner  R Manjarín  MR Amezcua  JC Domínguez  RN Kirkwood 《Reproduction in domestic animals》2014,49(6):899-902

The aim of this study was to determine the association between the oestrous response of pre‐pubertal gilts to gonadotrophin injection or boar exposure and their subsequent farrowing rate and litter size. At 154 days of age, randomly selected pre‐pubertal gilts received an intramuscular injection of 400 IU equine chorionic gonadotrophin plus 200 IU human chorionic gonadotrophin (PG600^®; Merck Animal Health; n = 181). From the remaining pool of animals not treated with hormones, the first gilts showing signs of oestrus were selected to act as controls (n = 201). Boar exposure began at 155 days of age for both groups, and gilts were bred at a weight of approximately 130 kg. Comparisons were made between PG600^®‐treated gilts exhibiting oestrus or not within 7 days post‐injection (early and late responders, respectively) and control gilts exhibiting oestrus or not within 30 days after beginning of boar exposure (select and non‐select control gilts, respectively). By 162 days, oestrus was detected in 67.5% of PG600^®‐treated gilts compared with 5.7% of control gilts (p < 0.0001). The proportion of animals observed in oestrus at least three times before breeding was greater for select control gilts compared with early and late responder PG600^®‐treated gilts (p ≤ 0.001). There were no significant differences in farrowing rate and litter size between the four treatment groups. These data indicate that PG600^® is an effective tool to induce an earlier oestrus in gilts, that subsequent farrowing rate and born alive litter size compare favourably to that of select gilts and that gilts failing to respond promptly to hormonal stimulation do not exhibit compromised fertility.  相似文献   

Shear strength of repacked remoulded samples of a calcareous soil as affected by long-term incorporation of three organic manures in central Iran     

A. Hemmat  N. AghilinateghM. Sadeghi 《Biosystems Engineering》2010,107(3):251-261

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Predicting perchlorate exposure in milk from concentrations in dairy feed     

Rice CP  Baldwin Vi RL  Abbott LC  Hapeman CJ  Capuco AV  Le A  Bialek-Kalinski K  Bannerman DD  Hare WR  Paape MJ  McCarty GW  Kauf AC  Sadeghi AM  Starr JL  McConnell LL  Van Tassell CP 《Journal of agricultural and food chemistry》2007,55(21):8806-8813

Perchlorate has been detected in U.S. milk samples from many different states. Applying data from a recently reported 9-week experiment in which 16 Holstein dairy cows were administered perchlorate allowed us to derive an equation for the dose-response relationship between perchlorate concentrations in feed/drinking water and its appearance in milk. Examination of background concentrations of perchlorate in the total mixed ration (TMR) fed in addition to the variable dose supplied to treated cows as a ruminal infusate revealed that cows receive significant and variable exposure to perchlorate from the TMR. Weekly examination of the TMR disclosed that a change in ingredients midway through the experiment caused a significant (78%) change in TMR perchlorate concentration. Analyses of the ingredients comprising the TMR revealed that 41.9% of the perchlorate came from corn silage, 22.9% came from alfalfa hay and 11.7% was supplied by sudan grass. Finally, USDA Food and Nutrition Survey data on fluid milk consumption were used to predict potential human exposure from milk that contained concentrations of perchlorate observed in our previous dosing study. The study suggests that reducing perchlorate concentration in dairy feed may reduce perchlorate concentrations in milk as well as the potential to reduce human exposure to perchlorate in milk.  相似文献