Antioxidant Activity of Brown Seaweeds     

Kajal Chakraborty  Anusree Maneesh  Fasina Makkar 《Journal Of Aquatic Food Product Technology》2017,26(4):406-419

Antioxidant potential of three brown seaweeds, Anthophycus longifolius, Sargassum plagiophyllum, and Sargassum myriocystum, obtained from the Gulf of Mannar region of India were evaluated utilizing different in vitro systems. Ethyl acetate (EtOAc) fraction of Anthophycus longifolius registered significantly greater hydroxyl radical scavenging ability (IC₅₀ 0.19 mg/mL) and was effective in stabilizing the 2,2′-azino-bis-3-ethylbenzothiozoline-6-sulfonic acid (IC₅₀ 1.23 mg/mL) and 1,1-diphenyl-2-picryl-hydrazil (DPPH) radicals (IC₅₀ 0.48 mg/mL) (p < 0.05). No significant differences in hydrogen peroxide (H₂O₂) scavenging and ferrous ion chelating properties of the EtOAc extracts of the seaweeds were apparent. The utilities of the reverse-phase high-performance liquid chromatography (RP-HPLC) method hyphenated to diode-array detection for analyzing the fingerprints of phenolic constituents in the solvent extracts and fractions of the seaweeds were denoted. High-performance liquid chromatographic analysis indicated the presence of phenolic acids in the solvent extracts of seaweeds. This study demonstrated the potential use of A. longifolius as candidate species to be used as a nutritional food supplement/functional foods to increase the shelf life of food items for human consumption.  相似文献   

Efficacy of various lipid supplements in formulated pellet diets for juvenile Scylla serrata     

Unniyampurath Unnikrishnan  Kajal Chakraborty  Rajaian Paulraj 《Aquaculture Research》2010,41(10):1498-1513

Efficacy of sunflower oil (diet SF) and soybean oil (diet SB) alone and in combination with cod liver oil (diets M1‐2.80:1.40:1.40, M2‐2.80:2.24:0.56 and M3‐2.80:0.56:2.24; cod liver oil:sunflower oil:soybean oil) as lipid supplements (5.6%) in formulated diets (crude fat ～9.79%) for juvenile Scylla serrata (weight=0.28±0.07 g, carapace width=9.7±0.1 mm) were compared with diet CL, containing cod liver oil alone as the lipid supplement (6 diets × 24 crabs stocked individually, randomized block design). Growth performance, nutrient (protein and lipid) intake and gain of crabs fed M1, M2 and M3 were higher (P≤0.05) than the crabs fed SF and SB, but were not significantly different (P≥0.05) from crabs fed CL. Dietary fatty acids (FAs) are found to influence the FA profile of test crabs. Higher tissue levels of 16:1n‐7, 18:1n‐9 and 18:1n‐7 reflected the essential FA deficiency in crabs fed diets supplemented only with vegetable oils. Results confirmed that S. serrata could utilize vegetable oil supplements in the formulated diets as a partial replacement (50%) of cod liver oil without compromising growth and survival. Partial substitution of marine fish oil with suitable vegetable oils can reduce the feed cost considerably, in the context of rising fish oil prices.  相似文献   

Bacillus thuringiensis vegetative insecticidal protein family Vip3A and mode of action against pest Lepidoptera     

Swapan Chakrabarty  Minghui Jin  Chao Wu  Panchali Chakraborty  Yutao Xiao 《Pest management science》2020,76(5):1612-1617

Vip3A proteins are widely used for controlling pest Lepidoptera. Different binding sites with different receptors in the insect midgut membrane and lack of cross‐resistance with crystal (Cry) proteins enhance their applicability, as both single proteins and proteins pyramided with Cry proteins in transgenic Bt crops. Vip3A proteins are effective but there is relatively little information about their structure, function, activation, specificity, and mode of action. In addition, the mechanism of insect resistance to these proteins is unknown. Phylogenetic analysis and multiple sequence alignment showed that Vip3A proteins are genetically distant from Cry proteins. The mode of action and insecticidal activity of Vip3A proteins are discussed in this review. This review also provides detailed information about the Vip3A protein family that may aid in the design of more efficient pest management strategies in response to insect resistance to insecticidal proteins. © 2020 Society of Chemical Industry  相似文献   

Preliminary Studies on Cryopreservation of Vegetative Thalli of Some Economically Important Seaweeds of India     

P. L. Lalrinsanga  S. K. Chakraborty  Alkesh Dwivedi  Nimai Barman  Niraj Kumar 《Journal Of Applied Aquaculture》2013,25(4):250-262

The effects of cryoprotectants and their concentrations, prefreezing temperature, equilibration time, thawing temperature, and the regenerative efficacy of cryopreserved vegetative thalli (apical tips) of Gracilaria corticata J. Agardh., Ulva lobata Duetzing, and Hypnea musiformis (Wulfar) Lamouroux were evaluated. The apical tips were suspended in various cryoprotective solutions and slowly cooled to ?40?C over a period of 4 h. After the slow cooling step, the suspensions were immediately immersed in liquid nitrogen (LN), which were thawed after 2 days. Both U. lobata and H. musiformis survived maximally in 10% DMSO, whereas 10% glycerol was found most suitable for G. corticata. A slow cooling temperature of ?40?C and thawing temperature of 40?C were found most suitable irrespective of the species. The equilibration time of 60 min was found most suitable for G. corticata, while 45 min was sufficient for both H. musiformi and U. lobata. Cryopreserved vegetative thalli were then successfully regenerated, though the survival was considerably reduced up to a period of 28 days after which it was stabilized.  相似文献   

Chemical hybridizing agents for chickpea (Cicer arietinum L.): leads from QSAR analysis of ethyl oxanilates and pyridones     

Chakraborty K  Devakumar C 《Journal of agricultural and food chemistry》2006,54(5):1868-1873

In the self-pollinated crops such as chickpea, induction of male sterility by deployment of chemical hybridizing agents (CHAs) facilitating "two-line" approach holds immense potential in heterosis breeding. A total of 40 test CHAs comprising 20 ethyl oxanilates and 20 pyridones were screened as potential CHAs on chickpea (variety BG 1088) at 500, 800, and 1000 ppm. Three test compounds mostly having either F (4)/Br (5)/CF(3) (19) at the para position of the aryl ring from a pool of 20 ethyl oxanilates were identified as the most potent CHAs causing >or=99% induction of pollen sterility and >90% total flower sterility at 1000-ppm test concentration. Among pyridone derivatives, N-(4-chlorophenyl)-5-carbethoxy-4,6-dimethyl, 1,2-dihydropyrid-2-one (26) was found to be the most active. Quantitative structure activity relationship (QSAR) analysis has revealed a direct involvement of Swain-Lupton field constant, F(p), with the target bioactivity which implied that field rather than resonance effect (R) had a positive effect on the activity. The real guiding principle for selectivity was found out to be the hydrophobic parameter pi value. The QSAR models indicated that increased steric bulk at the 4-position on the phenyl ring is associated with enhanced activity. The CHAs appeared to act by mimicking UDP-glucose, the key substrate in the synthesis of callose, or lead to an imbalance in acid-base equilibrium in pollen mother cells resulting in dissolution of callose wall by premature callase secretion.  相似文献   

Synthesis and characterization of N-acylaniline derivatives as potential chemical hybridizing agents (CHAs) for wheat (Triticum aestivum L.)     

Chakraborty K  Devakumar C 《Journal of agricultural and food chemistry》2006,54(18):6800-6808

Induction of male sterility by deployment of chemical hybridizing agents (CHAs) are important in heterosis breeding of self-pollinated crops like wheat, wherein the male and female organs are in the same flower. Taking a lead from the earlier work on rice, a total of 25 N-acylanilines comprising of malonanilates, acetoacetanilides, and acetanilides (including halogenated acetanilides) were synthesized and screened as CHAs on three genotypes of wheat, viz., PBW 343, HD 2046, and HD 2733 at 1500 ppm in the winter of 2001-2002. The N-acylanilines containing variations at the acyl and aromatic domain were synthesized by condensation of substituted anilines with appropriate diesters, acid chlorides, or monoesters. The test compounds with highly electronegative groups such as F/Br at the para position of the aryl ring were identified as the most potent CHAs, causing higher induction of male sterility. A variation of N-substitution at the side chain generally furnished analogues like 4'-fluoroacetoacetanilide (7) and ethyl 4'-fluoromalonanilate (1), which induced 89.12 and 84.66% male sterility, respectively, in PBW 343. Among halogenated acetanilides, the increasing number of chlorine atoms in the side chain led to an increase in the activity of 4'-fluoro (23) and 4'-bromo (24) derivatives of trichoroacetanilides, which induced >87% male sterility. Quantitative structure-activity relationship (QSAR) models indicated the positive contributions of the field effect exemplified by the Swain-Lupton constant (Fp) and negative contributions of the Swain-Lupton resonance constant (R) for the aromatic substitution. The positive influences of parachor (P) for the acyl domain have been underlined. These leads will be significant in explaining the CHA fit in the macromolecular receptor site. The CHAs appeared to act by causing an imbalance in the acid-base equilibrium in pollen mother cells resulting in dissolution of the callose wall by premature callase secretion.  相似文献   

Dietary Multispecies Probiotic Supplementation Enhances the Immunohematological Responses and Reduces Mortality by Aeromonas hydrophila in Labeo rohita Fingerlings     

Sipra Mohapatra  Tapas Chakraborty  Ashisa Kumar Prusty  Kurucheti Pani Prasad  Kedar Nath Mohanta 《Journal of the World Aquaculture Society》2014,45(5):532-544

A study was conducted to determine the immunomodulatory effect of probiotic mixture on the nonspecific immune response and disease resistance of Labeo rohita. Fish were fed with six different test diets with or without probiotics, namely, T1 (basal feed [BF] without probiotics), T2 (BF + Bacillus subtilis [BS] + Lactococcus lactis [LL]), T3 (BF + LL + Saccharomyces cerevisiae [SC]), T4 (BF + BS + SC), T5 (BF + BS + LL + SC), and T6 (BF + heat‐killed bacteria of BS + LL + SC). During the prechallenge period, significantly higher (P < 0.01) nitroblue tetrazolium assay (NBT), red blood cells (RBC), hemoglobin (Hb), globulin, and albumin content were recorded in the probiotic‐supplemented diet group. Feeding all the three probiotics significantly decreased (P < 0.01) the serum aspartate aminotransferases (AST), alanine aminotransferases (ALT), acid phosphatase (ACP), and alkaline phosphatase (ALP) activity and increased the myeloperoxidase (MPO) and immunoglobulin M (IgM) value. Significant difference in the NBT, Hb, serum albumin, and MPO was noticed in both prechallenged and postchallenged fish. Lowest survivability was recorded in the nonprobiotic fed group, followed by the group fed with heat‐killed probiotics, whereas the highest survivability was noticed in the group fed with all the three probiotics in live form. Thus, a probiotic mixture diet constituting of three probiotics in viable form is found to be optimum to enhance the immunity and postinfection survivability in L. rohita fingerlings.  相似文献   

Cell cycle-dependent coupling of the calcitonin receptor to different G proteins.   总被引：9，自引：0，他引：9  

M Chakraborty  D Chatterjee  S Kellokumpu  H Rasmussen  R Baron 《Science (New York, N.Y.)》1991,251(4997):1078-1082

Calcitonin is a calcium regulating peptide hormone with binding sites in kidney and bone as well as in the central nervous system. The mechanisms of signal transduction by calcitonin receptors were studied in a pig kidney cell line where the hormone was found to regulate sodium pumps. Calcitonin receptors activated the cyclic adenosine monophosphate (cAMP) or the protein kinase C (PKC) pathways. The two transduction pathways required guanosine triphosphate (GTP)-binding proteins (G proteins) (the choleratoxin sensitive Gs and the pertussis toxin sensitive Gi, respectively) and led to opposite biological responses. Moreover, selective activation of one or the other pathway was cell cycle-dependent. Therefore, calcitonin may induce different biological responses in target cells depending on their positions in the cell cycle. Such a modulation of ligand-induced responses could be of importance in rapidly growing cell populations such as during embryogenesis, growth, and tumor formation.  相似文献   

Relative Agronomic Effectiveness of phosphate rocks and P adsorption characteristics of an Oxic Rhodustalf in Eastern India     

Prabir K. Ghosal  Trishit Chakraborty  Buddhadeb Bhattacharya  Dipak K. Bagchi 《植物养料与土壤学杂志》2003,166(6):750-755

The Relative Agronomic Effectiveness (RAE) of rock phosphates as compared to water soluble Triple Super Phosphate was measured on direct, residual, and cumulative application of the P fertilizers in a field experiment with rice on an Oxic‐Rhodustalf in the eastern plateau region of India. The fertilizers were Morocco Rock Phosphate (MORP), Mussoorie Rock Phosphate (MRP), Partially Acidulated Rock Phosphate (PARP), and Triple Super Phosphate (TSP). The RAE of the rock phosphates were lower for direct application (54–80 %) and cumulative application (70–93 %) of P but roughly equal or larger for the residual effect (92–142 %) as compared to TSP. The P adsorption characteristic of the experimental soil conformed to the linear relationship of both Freundlich and Langmuir isotherm equation. The adsorption data when plotted according to Langmuir equation deviated from a single linear relationship at higher concentration (10 μg ml^–1), thereby giving two adsorption maximum values ( 68.49 μg g^–1 and 256.41 μg g^–1) and binding energies ( 2.86 ml μg^–1 and 0.089 ml μg^–1) for the soil. Two populations of P adsorption site with widely different affinity for P probably existed in the soil.  相似文献   

Populations of mycophagous and other amoebae in take-all suppressive and non-suppressive soils     

S. Chakraborty  J.H. Warcup 《Soil biology & biochemistry》1984,16(2):197-199

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