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41.
42.
Xu Gao Han Gil Choi Seo Kyoung Park Ju Hyoung Kim Ok Hwan Yu Ki Wan Nam 《Aquaculture Research》2019,50(3):856-861
Juvenile sporophytes and gametophytes of Ecklonia stolonifera were incubated in combinations of three pCO2 levels (360, 720 and 980 ppmv) and two temperatures (10 and 15°C for sporophytes; 15 and 20°C for gametophytes) to examine potential effects of climate change on photosynthesis and growth. Sporophytes had significantly higher maximum quantum yields (Fv/Fm) and maximum relative electron transport rates (rETRmax) at 720 ppmv than 360 and 980 ppmv. Also, these parameters were significantly lower at higher temperature of 15°C than at 10°C. Growth of female gametophytes was maximal at 360 ppmv rather than enriched pCO2 levels. Female gametophytes had significantly lower growth at higher temperature of 20°C than at 15°C. These results indicate effects of elevated pCO2 varied between generations: stimulating sporophytic photosynthesis and inhibiting gametophytic growth. Ocean acidification and warming would constitute a grave threat to seedling cultivation of E. stolonifera caused by growth inhibition of gametophytes at high pCO2 levels and temperatures. 相似文献
43.
Comparative evaluation of MCP gene in worldwide strains of Megalocytivirus (Iridoviridae family) for early diagnostic marker 下载免费PDF全文
A Mishra G‐H Nam J‐A Gim H‐E Lee A Jo D Yoon S Oh S Kim A Kim D‐H Kim Y C Kim H D Jeong H‐J Cha Y H Choi H‐S Kim 《Journal of fish diseases》2018,41(1):105-116
Members of the Iridoviridae family have been considered as aetiological agents of iridovirus diseases, causing fish mortalities and economic losses all over the world. Virus identification based on candidate gene sequencing is faster, more accurate and more reliable than other traditional phenotype methodologies. Iridoviridae viruses are covered by a protein shell (capsid) encoded by the important candidate gene, major capsid protein (MCP). In this study, we investigated the potential of the MCP gene for use in the diagnosis and identification of infections caused Megalocytivirus of the Iridoviridae family. We selected data of 66 Iridoviridae family isolates (53 strains of Megalocytivirus, eight strains of iridoviruses and five strains of Ranavirus) infecting various species of fish distributed all over the world. A total of 53 strains of Megalocytivirus were used for designing the complete primer sets for identifying the most hypervariable region of the MCP gene. Further, our in silico analysis of 102 sequences of related and unrelated viruses reconfirms that primer sets could identify strains more specifically and offers a useful and fast alternative for routine clinical laboratory testing. Our findings suggest that phenotype observation along with diagnosis using universal primer sets can help detect infection or carriers at an early stage. 相似文献
44.
To develop reusable incontinence products, blend nonwovens of hollow viscose rayon (HVR) and super absorbent fibers (SAFs) were prepared using a needle-punching process and their liquid handling properties, such as the fluid absorption capacity, fluid retention capacity, fluid absorption under load, moisture evaporation rate, and repeated water absorption were investigated. As the SAF content in the HVR/SAF blend nonwovens was increased, the fluid absorption capacity, fluid retention capacity, and fluid absorption under load increased, whereas the moisture evaporation rate decreased. SAF had a more significant effect on fluid retention than fluid absorption. In the case of HVR/SAF(8/2) and HVR/SAF(7/3), more than 100 % of the fluid absorption capacity was retained even after 5 cycles of repeated water absorption tests. Overall, the HVR/SAF blend nonwovens are good candidates for reusable incontinence products. 相似文献
45.
Woo Jung Kim Young Ok Kim Jin Hee Kim Bo-Hye Nam Dong-Gyun Kim Cheul Min An Jun Sik Lee Pan Soo Kim Hye Min Lee Joa-Sup Oh Jong Suk Lee 《Marine drugs》2016,14(1)
The ocean is a rich resource of flora, fauna, and food. A wild-type bacterial strain showing confluent growth on marine agar with antibacterial activity was isolated from marine water, identified using 16S rDNA sequence analysis as Pseudoalteromonas sp., and designated as strain M2. This strain was found to produce various secondary metabolites including quinolone alkaloids. Using high-resolution mass spectrometry (MS) and nuclear magnetic resonance (NMR) analysis, we identified nine secondary metabolites of 4-hydroxy-2-alkylquinoline (pseudane-III, IV, V, VI, VII, VIII, IX, X, and XI). Additionally, this strain produced two novel, closely related compounds, 2-isopentylqunoline-4-one and 2-(2,3-dimetylbutyl)qunoline-4-(1H)-one, which have not been previously reported from marine bacteria. From the metabolites produced by Pseudoalteromonas sp. M2, 2-(2,3-dimethylbutyl)quinolin-4-one, pseudane-VI, and pseudane-VII inhibited melanin synthesis in Melan-A cells by 23.0%, 28.2%, and 42.7%, respectively, wherein pseudane-VII showed the highest inhibition at 8 µg/mL. The results of this study suggest that liquid chromatography (LC)-MS/MS-based metabolite screening effectively improves the efficiency of novel metabolite discovery. Additionally, these compounds are promising candidates for further bioactivity development. 相似文献
46.
Hwa Yeun Nam Brad Coates Kyung Seok Kim Marana Park Joon-Ho Lee 《Journal of insect science (Online)》2015,15(1)
The white-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae), is a major pest of rice and has long-range migratory behavior in Asia. Microsatellite markers (simple sequence repeats) have been widely used to determine the origins and genetic diversity of insect pests. We identified novel microsatellite loci for S. furcifera samples collected from Laos, Vietnam, and three localities in Bangladesh from next-generation Roche 454 pyrosequencing data. Size polymorphism at 12 microsatellite loci was verified for 40 adult individuals collected from Shinan, South Korea. The average number of alleles per locus was 7.92. The mean values of observed (Ho) and expected heterozygosities (HE) were 0.615 and 0.757, respectively. These new microsatellite markers will be a resource for future ecological genetic studies of S. furcifera samples across more broad geographic regions in Asia and may assist in estimations of genetic differentiation and gene flow among populations for implementation of more effective management strategies to control this serious rice pest. 相似文献
47.
So Young Kim Myoung-Sook Shin Geum Jin Kim Hyukbean Kwon Myong Jin Lee Ah-Reum Han Joo-Won Nam Chan-Hun Jung Ki Sung Kang Hyukjae Choi 《Marine drugs》2021,19(8)
The epithelial–mesenchymal transition (EMT) of cancer cells is a crucial process in cancer cell metastasis. An Aquimarina sp. MC085 extract was found to inhibit A549 human lung cancer cell invasion, and caprolactin C (1), a new natural product, α-amino-ε-caprolactam linked to 3-methyl butanoic acid, was purified through bioactivity-guided isolation of the extract. Furthermore, its enantiomeric compound, ent-caprolactin C (2), was synthesized. Both 1 and 2 inhibited the invasion and γ-irradiation-induced migration of A549 cells. In transforming growth factor-β (TGF-β)-treated A549 cells, 2 inhibited the phosphorylation of Smad2/3 and suppressed the EMT cell marker proteins (N-cadherin, β-catenin, and vimentin), as well as the related messenger ribonucleic acid expression (N-cadherin, matrix metalloproteinase-9, Snail, and vimentin), while compound 1 did not suppress Smad2/3 phosphorylation and the expression of EMT cell markers. Therefore, compound 2 could be a potential candidate for antimetastatic agent development, because it suppresses TGF-β-induced EMT. 相似文献
48.
Jin-Joo Hue Ki Nam Lee Jae-Hwang Jeong Sang-Hwa Lee Young Ho Lee Seong-woon Jeong Sang Yoon Nam Young Won Yun Beom Jun Lee 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(3):189-195
This study was to investigate the anti-obesity effects of diglyceride (DG)-conjugated linoleic acid (CLA) containing 22% CLA as fatty acids in C57BL/6J ob/ob male mice. There were four experimental groups including vehicle control, DG, CLA, and DG-CLA. The test solutions of 750 mg/kg dose were orally administered to the mice everyday for 5 weeks. CLA treatments significantly decreased mean body weight in the obese mice throughout the experimental period compared to the control (p < 0.01). All test solutions significantly decreased the levels of triglyceride, glucose and free fatty acids in the serum compared with control (p < 0.05). The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05). CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05). DG and DG-CLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05). A remarkable decrease in the number of lipid droplets and fat globules was observed in the livers of mice treated with DG, CLA, and DG-CLA compared to control. Treatments of DG and CLA actually increased the expression of peroxisome proliferator-activated receptor gamma. These results suggest that DG-CLA containing 22% CLA have a respectable anti-obesity effect by controlling serum lipids and fat metabolism. 相似文献
49.
In this study, micro-porous carboxymethyl cellulose (CMC) nonwovens were prepared by carboxymethylation of cellulose nonwovens
produced by a wet-laid process and their properties were investigated for potential applications as adhesion prevention barriers.
After carboxymethylation, the thickness and mean pore size of the cellulose nonwovens were increased, whereas their pore size
distribution became narrower. Tensile strength of cellulose nonwovens was proportional to basis weight, and dramatically increased
after carboxymethylation. CMC nonwovens immediately absorbed a phosphate buffered saline solution and showed swollen phase
within 1 min. It was found that the thickness and pore size distribution of CMC nonwovens could be easily controlled by the
wet-laid process. It is expected that the CMC nonwovens can be used as adhesion prevention barriers. 相似文献
50.