Primary sinonasal malignant melanoma with systemic metastasis in a non-gray horse     

Hitoshi Hatai  Takashi Hatazoe  Haruka Seo  Teruaki Tozaki  Shingo Ishikawa  Noriaki Miyoshi  Kazuhiro Misumi  Seiji Hobo 《Journal of veterinary diagnostic investigation》2021,33(2):379

A 27-y-old Anglo-Arabian gelding with bay coat color was presented with a swelling of the left maxillary region. Fenestration on the left maxilla revealed that the left maxillary sinus was filled with black-red tissue. A portion of the tissue was excised and diagnosed histologically as malignant melanoma. Genotyping of the STX17 gene for gray coat color revealed that the horse did not have the “gray” factor. The horse was euthanized ~3 mo after first presentation. During autopsy, a black-to-gray mass extended from the left nasal cavity to the surrounding paranasal sinus and invaded the hard palate, cribriform plate, and the cranial portion of the left olfactory bulb. Moreover, identical black nodules were present in lymph nodes from the mandible to the larynx, and in the spleen, liver, kidney, and adrenal glands. However, masses were not found in the skin, perineal region, or pelvic cavity. All of the black-to-gray nodules were malignant melanomas that were histologically identical to the initial biopsy; tumor emboli were also found in the kidney. Sinonasal mucosal melanoma is a rare disease in horses.  相似文献   

Effects of soybean meal supplementation on ruminal kinetics of feed particle size reduction and passage in dairy cows fed rice straw     

Kyaw San Win  Koichiro Ueda  Seiji Kondo 《Animal Science Journal》2021,92(1):e13590

Six ruminal-cannulated nonlactating Holstein Friesian cows (mean body weight:660 ± 42.9 kg) were used to investigate the effect of soybean meal (SBM) supplementation on voluntary rice straw (RS) intake, feed particle size reduction, and passage kinetics in the rumen. They were allocated to two dietary treatments: RS alone or RS supplemented with SBM. Voluntary dry matter intake of RS and total tract fiber digestibility was increased by SBM supplementation (p < 0.05). Supplementation with SBM decreased rumination time per dietary dry matter (DM) and neutral detergent fiber (NDFom) intake (p < 0.01). Particle size distribution in the rumen and total ruminal NDFom digesta weights were not affected by SBM supplementation. However, the disappearance rates of total digesta and large and small particles from the rumen were increased by SBM supplementation (p < 0.01). Moreover, SBM supplementation increased the rate of size reduction in ruminal particles (p < 0.05). In situ disappearance of DM and NDFom of RS in the rumen was greater in SBM-supplemented cows than in nonsupplemented cows (p < 0.05). This study clearly showed that increased ruminal RS particle size reduction, passage, and fermentation due to SBM supplementation accelerated the RS particle clearance from the rumen and resulted in increased voluntary RS intake of dairy cows.  相似文献   

Artificial incubation of Japanese crayfish (Cambaroides japonicus) eggs by using a simple, easy method with a microplate     

Kazuyoshi Nakata  Hajime Matsubara  Seiji Goshima 《Aquaculture (Amsterdam, Netherlands)》2004,230(1-4):273-279

We developed a simple, easy method with a microplate to artificially incubate Japanese crayfish (Cambaroides japonicus) eggs for their cultivation. We prepared 6-, 12-, 24-, 48-, and 96-well microplates containing sterile water heated to 5, 10, 15, and 20 °C. Fourteen experimental groups for each water temperature were prepared for each of different water volumes (0.125–10 ml) in each well. One embryonic egg was placed in each well. Experiments were also conducted with water collected from the lake where the eggs were harvested from and held at 15 °C. In the microplates with sterile water, high proportions of eggs hatched (60–100%) at 15 °C in all volumes of water, although the proportions of hatching were low (0–20%) at 5, 10, and 20 °C. All eggs died in the experiments that used lake water. We conclude that the 96-well would be the most effective size to hatch crayfish eggs in, because of its convenience. This method using a microplate is simpler and easier compared with methods of previous studies to artificially incubate crayfish eggs, and therefore it might be useful to incubate eggs of other freshwater crayfish species.  相似文献   

Identification of alfonsino, Beryx mollis and B. splendens collected in Japan, based on the mitochondrial cytochrome b gene, and their comparison with those collected in New Caledonia     

Seiji Akimoto  Shiro Itoi  Keijiro Sezaki  Philippe Borsa  Shugo Watabe 《Fisheries Science》2006,72(1):202-207

ABSTRACT:   The sequences spanning 307 bp of the mitochondrial cytochrome b gene were determined for 45 sepcimens of Beryx splendens and seven specimens of B. mollis collected in Japan, resulting in identification of 11 and three haplotypes in the two species, respectively. The parsimony tree was constructed from the determined sequences and those registered into the GenBank database as species A and W of B. splendens collected in New Caledonia, featuring with two clades. The first clade comprised species W from New Caledonia and B. mollis in the present study, whereas the second one contained species A from New Caledonia and B. splendens in the present study. These results demonstrate a large geographic distribution for both B. splendens and B. mollis. Some of the haplotypes found in Japan were identical to those of New Caledonia for both B. mollis and B. splendens , suggesting levels of gene flow at the trans -oceanic scale.  相似文献   

Inheritance of low pasting temperature in sweetpotato starch and the dosage effect of wild-type alleles     

Kenji Katayama  Seiji Tamiya  Tetsufumi Sakai  Yumi Kai  Akiko Ohara-Takada  Toshikazu Kuranouchi  Masaru Yoshinaga 《Breeding Science》2015,65(4):352-356

Sweetpotato (Ipomoea batatas (L.) Lam.), which is an outcrossing hexaploid, is one of the most important starch-producing crops in the world. During the last decade, new sweetpotato cultivars, e.g. ‘Quick Sweet’, which have approximately 20°C lower pasting temperature, slower retrogradation and higher digestibility of raw starch than ordinary cultivars, have been developed in Japan. Genetic analysis of these variants with low pasting temperature starch was conducted in this study. Using 8 variants and 15 normal clones, 26 families were generated. The results from analyzing these progenies suggested that this trait is a qualitative character controlled by one recessive allele (designated spt), which is inherited in a hexasomic manner. A dosage effect of the wild-type Spt allele was found for starch pasting temperature, although the effect was not linear. These results will aid breeders to develop sweetpotato cultivars with a range of starch pasting temperatures.  相似文献   

Segment formation of Enchytraeus japonensis (Oligochaeta: Enchytraeidae): The 7th international symposium on earthworm ecology · Cardiff · Wales · 2002     

Makoto Honda  Tomoharu Suzuki  Seiji Matsumoto  Shinobu Gamou   《Pedobiologia》2003,47(5-6):522-525

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Immunohistochemical study of matrix metalloproteinase‐3 (MMP‐3) at the articular cartilage in osteochondrotic pigs     

Hiroyoshi OHATA  Kenji ZUSHIDA  Toshie SUGIYAMA  Seiji KUSUHARA 《Animal Science Journal》2002,73(6):517-522

In order to understand the mechanism of osteochondrosis in the pig, articular cartilage was taken from the distal femoral condyles of Duroc pigs exhibiting leg weakness and then examined immunohistochemically for the localization of matrix metalloproteinases‐3 (MMP‐3), one of the enzymes involved in the resolution of cartilage matrix. The articular cartilage had the typical characteristics of osteochondrotic lesions, such as abnormal calcification, clefts of cartilage, disappearance of proteoglycan, and necrotic chondrocytes. The immunoreaction of MMP‐3 was observed in chondrocytes at the boundary between normal cartilage and proteoglycan‐deficient area. Moreover, chondrocytes expressing MMP‐3 showed normal morphology, but the surrounding cartilage matrix did not stain with toluidine blue, which indicated a lack of glycosaminoglycans. These results suggest that MMP‐3 is highly involved in the appearance and expansion of osteochondrotic lesions.  相似文献   

Effects of pre-shipping marbofloxacin administration on fever and blood properties in healthy Thoroughbreds transported a long distance     

Yoshiro ENDO  Takeru TSUCHIYA  Takaya OMURA  Kenji NAKAI  Kenji KOROSUE  Mutsuki ISHIMARU  Yuhiro ISHIKAWA  Seiji HOBO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):75-79

The present study evaluated the effects of single-dose marbofloxacin in protecting horses against fever associated with transportation using 48 healthy Thoroughbreds. All horses were premedicated with interferon-α (0.5 U/kg, sublingually, every 24 hr) for 2 days before transportation and on the day of transportation. Horses were randomly assigned to receive marbofloxacin (2 mg/kg, IV, once; MRFX group), enrofloxacin (5 mg/kg, IV, once; ERFX group) or saline (0.9% NaCl) solution (10 ml, IV, once; control group) ≤1 hr before being transportation. Each group contained 16 horses (8 males, 8 females). Horses were transported 1,210 km using commercial vans over the course of approximately 26 hr. Clinical examinations and hematologic analyses were performed on all horses both before and after transportation. Post-transportation neutrophil to lymphocyte ratios were significantly lower in horses in the MRFX group compared with the control horses. The serum amyloid A levels were significantly lower in horses in the MRFX group and ERFX group compared with the control horses. Regarding the post-transportation rectal temperatures, fever was detected in 0 horses and 1 horse in the MRFX and ERFX groups, respectively, whereas fevers exceeding 39.1°C were detected in 2 horses in the control group. Additionally, the number of essential post-transportation treatments provided by veterinarians was reduced 3-fold in the MRFX and ERFX groups compared with the saline group. MRFX provided ERFX-like protection against fever associated with long-distance transportation, yielding significantly better protection than saline. Administration of MRFX just before transportation deserves a further study for efficacy in preventing horse fever associated with transportation.  相似文献   

Effects of grass hay proportion in a corn silage‐based diet on rumen digesta kinetics and digestibility in dairy cows          下载免费PDF全文

Kyaw San Win  Koichiro Ueda  Seiji Kondo 《Animal Science Journal》2015,86(9):833-841

In this study, we aimed to evaluate the effects of six levels of orchardgrass hay (GH) proportion (0%, 10%, 20%, 30%, 40% or 50% of dry matter) in finely chopped corn silage (CS)‐based diets on digesta kinetics of CS and GH in the rumen. Six non‐lactating, rumen‐cannulated Holstein cows were used in a 6 × 6 Latin square design. Ruminal digesta kinetics was measured by ruminal dosing of feed particle markers (dysprosium for CS, erbium for GH) followed by fecal sampling. The increase of GH proportion had a quadratic effect (P < 0.01) on total tract digestibility of neutral detergent fiber (NDF) and acid detergent fiber. The proportion of GH did not affect the particle size distribution of rumen digesta, total weight of dry matter or NDF in the rumen. The rates of large particle size reduction in the rumen for CS tended to increase linearly with increasing GH proportion (P = 0.077). A quadratic effect (P < 0.05) was found with increasing the GH proportion for the ruminal passage rate of small GH particles, but not for CS particles. The results suggested that associative effects between CS and GH could be generated on rumen digesta kinetics when cows were fed a CS‐based diet with an increased proportion of GH.  相似文献   

Effects of copper on the decomposition of plant residues,microbial biomass,and β-glucosidase activity in soils     

Masakazu Aoyama  Seiji Itaya  Michizo Otowa 《Soil Science and Plant Nutrition》2013,59(3):557-566

Abstract

Two types of soils (Brown Lowland soil and Ando soil), which were artificially enriched with different amounts of Cu, were incubated with or without pulverized orchard grass for 12 weeks at 25°C. For both soils with and without orchard grass amendment, the amount of CO₂ evolved over the 12-week period of incubation decreased by the enrichment with Cu at a concentration exceeding 1,000 mg kg^?1 soil. The decrease of the mineralization of added orchard grass in the Cu-enriched soil was conspicuous especially during the initial period of incubation. The amount of microbial biomass C at the end of the incubation was significantly reduced by the Cu enrichment regardless of the amendment with orchard grass. The relative decrease of the soil microbial biomass was much greater than that of the soil respiration. The amount of biomass C was negatively correlated with the amount of 0.1 M CaCl₂-extractable Cu as a logarithmic function. On the other hand, the β-glucosidase activity at the end of the incubation was not significantly affected by the presence of Cu in the soils without orchard grass amendment and increased with the increase in the amount of enriched Cu in the orchard grass-amended soils.  相似文献