Growth,Reproductive Performance,Carcass Characteristics and Meat Quality in F1 and F2 Progenies of Somatic Cell-Cloned Pigs     

Noritaka ADACHI  Daisuke YAMAGUCHI  Akiyuki WATANABE  Narumi MIURA  Seiji SUNAGA  Hitoshi OISHI  Michiko HASHIMOTO  Takatsugu OISHI  Masaki IWAMOTO  Hirofumi HANADA  Masanori KUBO  Akira ONISHI 《The Journal of reproduction and development》2014,60(2):100-105

The objective of this study was to examine the health and meat production of cloned sows and their progenies in order to demonstrate the application of somatic cell cloning to the pig industry. This study compared the growth, reproductive performance, carcass characteristics and meat quality of Landrace cloned sows, F1 progenies and F2 progenies. We measured their body weight, growth rate and feed conversion and performed a pathological analysis of their anatomy to detect abnormalities. Three of the five cloned pigs were used for a growth test. Cloned pigs grew normally and had characteristics similar to those of the control purebred Landrace pigs. Two cloned gilts were bred with a Landrace boar and used for a progeny test. F1 progenies had characteristics similar to those of the controls. Two of the F1 progeny gilts were bred with a Duroc or Large White boar and used for the progeny test. F2 progenies grew normally. There were no biological differences in growth, carcass characteristics and amino acid composition among cloned sows, F1 progenies, F2 progenies and conventional pigs. The cloned sows and F1 progenies showed normal reproductive performance. No specific abnormalities were observed by pathological analysis, with the exception of periarteritis in the F1 progenies. All pigs had a normal karyotype. These results demonstrate that cloned female pigs and their progenies have similar growth, reproductive performance and carcass quality characteristics and that somatic cell cloning could be a useful technique for conserving superior pig breeds in conventional meat production.  相似文献   

Effects of temperature on food consumption, growth and oxygen consumption of freshwater prawn Macrobrachium rosenbergii (de Man 1879) postlarvae     

Cuijuan Niu  Daxong Lee  Seiji Goshima  & Shigeru Nakao 《Aquaculture Research》2003,34(6):501-506

Effects of temperature on food consumption, growth and oxygen consumption were estimated for the freshwater prawn Macrobrachium rosenbergii postlarvae at 23 °C, 28 °C and 33 °C in the laboratory. The results showed that the animal's initial body weight had a close linear relationship with food consumption and growth. Food consumption increased directly with temperature. Consumption rates (C; mg day^?1 ind^?1 ) of the 28 °C and 33 °C groups were much higher than that of the 23 °C group (P < 0.001), and the 33 °C group's consumption rate was higher than that of the 28 °C group (P < 0.05). The relationship of food consumption with temperature and initial body weight (W; mg) could be described as: C = 0.0679W + 0.185t? 3.17. Growth increased significantly with increased temperature. The relationship among specific growth rate, temperature and initial body weight was as follows: SGR = ?0.110W + 0.213t + 0.176. However, temperature showed no effect on growth efficiency. Oxygen consumption increased significantly with temperature (P < 0.01). The weight‐specific oxygen consumption rates (mg O₂ g^?1 h^?1) at 23 °C, 28 °C and 33 °C were 0.83, 1.16 and 1.49 mg O₂ g^?1 h^?1 for 61.92 mg M. rosenbergii.  相似文献   

Comparison of the Occurrence of Transportation-associated Fever in 2 Years Old Thoroughbreds before and after Introduction of Prophylactic Marbofloxacin Administration     

Yoshiro ENDO  Takeru TSUCHIYA  Kentaro AKIYAMA  Naoya TAKEBE  Kenji NAKAI  Kenji KOROSUE  Mutsuki ISHIMARU  Nao TSUZUKI  Seiji HOBO 《Journal of Equine Science》2014,25(4):79-81

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Increase in plasma total antioxidant capacity of grazing Japanese Black heifers and cows in forestland in Japan     

Satoshi Haga  Hiroshi Ishizaki  Miwa Nakano  Seiji Nakao  Kiyoshi Hirano  Yoshito Yamamoto  Miya Kitagawa  Hiroyuki Sasaki  Yoshihiro Kariya 《Animal Science Journal》2014,85(2):135-142

Blood total antioxidant capacity (TAC) has become a key bio‐marker for animal health. Forest‐grazing cattle are known to forage various native plants that have high TAC. This study evaluated differences of plasma TAC between forest‐grazing (FG) and pasture‐grazing cattle (PG). Experiment 1 monitored the plasma TAC levels of 32 Japanese Black cattle. The level in PG did not change throughout the grazing period. However, that in FG, which increased from summer, was significantly higher than that in PG through fall (P < 0.05). In experiment 2, we used nine Japanese Black heifers and investigated their blood antioxidant parameters and the TAC in plants that the cattle consumed in late June and September. The plasma TAC levels in FG were significantly higher than those in PG in both periods (P < 0.05). Plasma levels of lipid peroxidation in FG tended to be lower than that in PG (P = 0.098). Furthermore, the TAC levels in various species of shrubs and trees consumed by FG were higher than those in pasture grasses. Results of this study show that plasma TAC of grazing Japanese Black cattle in forestland increase from summer through fall.  相似文献   

Identification of QTLs and candidate genes for days to heading in an upland New Rice for Africa (NERICA) variety     

Yohei Koide  Akiko Kawasaki-Tanaka  Theophile Odjo  Mitsuhiro Obara  Seiji Yanagihara  Yoshimichi Fukuta 《Euphytica》2015,203(1):153-164

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Reductions in greenhouse gas emissions by using wood to protect against soil liquefaction     

Chihiro Kayo  Seiji Hashimoto  Atsunori Numata  Masanori Hamada 《Journal of Wood Science》2011,57(3):234-240

We compared the greenhouse gas (GHG) emissions from a log pile (LP) to those from a sand compaction pile (SCP) and from cement deep mixing (CDM) as measures against soil liquefaction, assuming that forest and waste management scenarios influence the GHG (CO₂, CH₄, and N₂O) balance of wood. We found little difference between the LP and SCP methods with respect to GHG emissions from fossil fuel and limestone consumption. However, GHG emissions from the CDM method were seven times higher than emissions from the LP method. In the GHG balance of wood, when the percentage of CH₄ emissions from carbon in underground wood was lower than 3.3%, permanent storage in the log achieved greater reductions in GHG emissions than using the waste log as fuel in place of coal or heavy oil. In order to obtain reductions in GHG emissions by replacing SCPs or CDM with LPs, sustainable forest management with reforestation and prevention of CH₄ emissions from the underground log are essential. Using reforestation, permanent storage of the log, no CH₄ emission from the log, and using logging residues instead of coal, the LP can achieve reductions in GHG emissions of 121 tonnes of CO₂ per 100 m² of improvement area by replacing CDM.  相似文献   

Trophodynamic structure of a swampy bog at the climax stage of limnological succession III. Filamentous fungi associated with the standing leaves of Typha latifolia     

Seiji Tokumasu 《Water, air, and soil pollution》1994,76(3-4):491-499

The filamentous fungus flora on standing leaves of Typha latifolia in a bog was followed from late spring to early winter. A seasonal successional change of fungal colonizers was observed. Certain groups of species tended to be specific to a particular physiological condition of leaves. Leaf parasites and common primary saprophytes were major colonizers on the developing leaves. After the flowering time of host plant, facultative parasites invaded the aged leaves and finally became dominant on the dead leaves in early winter. A species of Fusarium, F. chlamydosporum was recognized as a main decomposer of the dead leaves near the water line in 1992. It may largely contribute to a smooth supply of dead aerial leaves for the heterotrophic community in the water.  相似文献   

Development of a multiplex PCR method for simultaneous detection of diagnostic DNA markers of five disease and pest resistance genes in potato     

Kazuyuki?Mori  Yu?Sakamoto  Nobuhiro?Mukojima  Seiji?Tamiya  Takashi?Nakao  Takashige?Ishii  Kazuyoshi?HosakaEmail author 《Euphytica》2011,180(3):347-355

Multiplex PCR is practically a reasonable choice for molecular marker-assisted selection in potato breeding. We had developed and were using a multiplex PCR method for selection of resistance genes to cyst nematode (H1), Potato virus X (Rx1) and late blight (R1 and R2). Since then, more reliable and tightly linked markers for H1 and R2, and a new marker for resistance to Potato virus Y (Ry _chc ) were developed. In this article, all these superior markers, including a positive marker to eliminate PCR-failed samples, were incorporated into one multiplex PCR assay. Using the newly developed multiplex PCR technique, five plants potentially harboring all five resistance genes were selected from 96 hybrid plants approximately 5 h after DNA extraction, which is a third of the operation time compared with separate PCR reactions for each marker.  相似文献   

Talin binding to integrin beta tails: a final common step in integrin activation     

Tadokoro S  Shattil SJ  Eto K  Tai V  Liddington RC  de Pereda JM  Ginsberg MH  Calderwood DA 《Science (New York, N.Y.)》2003,302(5642):103-106

Control of integrin affinity for ligands (integrin activation) is essential for normal cell adhesion, migration, and assembly of an extracellular matrix. Integrin activation is usually mediated through the integrin beta subunit cytoplasmic tail and can be regulated by many different biochemical signaling pathways. We report that specific binding of the cytoskeletal protein talin to integrin beta subunit cytoplasmic tails leads to the conformational rearrangements of integrin extracellular domains that increase their affinity. Thus, regulated binding of talin to integrin beta tails is a final common element of cellular signaling cascades that control integrin activation.  相似文献   

Newsmaker interview: Seiji Yasumura. Fukushima begins 30-year odyssey in radiation health. Interview by Dennis Normile     

Yasumura S 《Science (New York, N.Y.)》2011,333(6043):684-685

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