Development of a pooled probe method for locating small gene families in a physical map of soybean using stress related paralogues and a BAC minimum tile path     

Kay L Shopinski  Muhammad J Iqbal  Jeffry L Shultz  Dheepakkumaran Jayaraman  David A Lightfoot 《Plant methods》2006,2(1):20-13

Background  

Genome analysis of soybean (Glycine max L.) has been complicated by its paleo-autopolyploid nature and conserved homeologous regions. Landmarks of expressed sequence tags (ESTs) located within a minimum tile path (MTP) of contiguous (contig) bacterial artificial chromosome (BAC) clones or radiation hybrid set can identify stress and defense related gene rich regions in the genome. A physical map of about 2,800 contigs and MTPs of 8,064 BAC clones encompass the soybean genome. That genome is being sequenced by whole genome shotgun methods so that reliable estimates of gene family size and gene locations will provide a useful tool for finishing. The aims here were to develop methods to anchor plant defense- and stress-related gene paralogues on the MTP derived from the soybean physical map, to identify gene rich regions and to correlate those with QTL for disease resistance.  相似文献   

Equid herpesvirus 1 is neurotropic in mice,but latency from which infectious virus can be reactivated does not occur     

Iqbal J  Edington N 《Acta veterinaria Hungarica》2002,50(1):117-129

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Assessing yield optimization and water reduction potential for summer-sown and spring-sown maize in Pakistan     

M. Anjum Iqbal  G. Bodner  J. Eitzinger 《Agricultural Water Management》2010,97(5):731-737

Agricultural food production in arid and semi-arid regions faces the challenge to ensure high yields with limited supply of water. This raises the question to which extent irrigation supply can be reduced without detriment to yield. Our study focuses on the yield-water uptake relationship for maize in the moderate water stress range in order to determine the onset of stress-induced dry-matter and yield losses. Compensatory plant responses under moderate stress levels are discussed in relation to seasonal climatic conditions.Summer-sown and spring-sown maize were irrigated with a decreasing amount of water in a field experiment in Pakistan. Water supply ranged from 100% water required to maintain soil at field capacity (FC) to 40% of FC. The average dry-matter and yield levels were slightly higher for summer-sown (15.0 Mg ha⁻¹) compared to spring-sown maize (13.1 Mg ha⁻¹). The onset of significant dry-matter and yield reduction started at the least irrigation treatment in both seasons. The amount of water required to avoid production losses was 272 mm in the summer-sown maize during the autumn growing season, and 407 mm for the spring-sown maize in the summer season, when the evaporative demand of the atmosphere was +27% higher. Water use efficiency (WUE_ET), normalized by vapour pressure deficit, of the summer-sown maize which was 10.0 kg kPa m⁻³, was +15% higher compared to the spring-sown crop; while the irrigation water productivity (2.9 kg m⁻³) was +11% more. WUE_ET increased over the whole range of applied water deficits for summer-sown maize, while the spring-sown crop showed a decreasing WUE_ET in the less irrigated treatment. Due to the higher efficiency in summer-sown maize, the potential in irrigation reduction without production losses (129 mm) was higher compared to the spring-sown maize (57 mm). Our results showed that in Pakistan water saving irrigation practices can be applied without yield loss mainly during the cooler growing season when the crop can efficiently compensate a lower total water uptake by increased use efficiency. For spring-sown maize the increasing evaporative demand of the atmosphere towards summer implies a higher risk of yield losses and narrows the range to exploit higher irrigation water productivity under moderate water deficit conditions.  相似文献   

Using yield quantitative trait locus targeted SSR markers to study the relationship between genetic distance and yield heterosis in upland cotton (Gossypium hirsutum)     

Xue Li  Kashif Shahzad  Liping Guo  Tingxiang Qi  Xuexian Zhang  Hailin Wang  Huini Tang  Xiuqin Qiao  Jinfa Zhang  Jianyong Wu  Chaozhu Xing 《Plant Breeding》2019,138(1):105-113

The objective of this study was to examine the relationship between heterosis and genetic distance in upland cotton (Gossypium hirsutum L.). Heterosis in the different environments was evaluated and the relationship between heterosis and genetic distance (GD) was determined based on SSR markers for yield quantitative trait loci (QTL). Yields of seed cotton and lint showed a linear relationship with mid‐parent heterosis (MPH) and better‐parent heterosis (BPH). The variation in heterosis for other traits and their correlation with GD may be due to environmental factors or the effort of QTLs tested in the present study may vary in different environments. The present findings provide a foundation for heterotic grouping of parental lines and breeding of new cotton hybrids with improved seed cotton yield. This study calls for more research with stable QTLs as well as advance molecular marker techniques may be used in predicting yield heterosis in a more precise and reliable manner.  相似文献   

Biocidal radiuses of cycloxaprid,imidacloprid and lambda‐cyhalothrin droplets controlling against cotton aphid (Aphis gossypii) using an unmanned aerial vehicle     

Shahzad Ali Nahiyoon  Li Cui  Daibin Yang  Xiaojing Yan  Changhui Rui  Huizhu Yuan 《Pest management science》2020,76(9):3020-3029

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Allelic Variation within Single Podded Gene Characterized by STMS Marker in Chickpea     

Chickpea 《分子植物育种》2007,5(2):252-254

Chickpea （Cicer arietinum L.）, is an important grain legume crop throughout the world especially in developing countries. However the average yield worldwide is considered to be lower than its potential yield （Singh et al., 1994）. The average yield of chickpea is much lower in Pakistan, which is about 5500-650 kg/hm^2 due to various abiotic and biotic stresses （Shah et al., 2005）.  相似文献   

Evidence for Resistance to Bacillus thuringiensis (Bt) subsp. kurstaki HD-1, Bt subsp. aizawai and Abamectin in Field Populations of Plutella xylostella from Malaysia     

Muhammad Iqbal  Robert H. J. Verkerk  Michael J. Furlong  Pek Choo Ong  Syed Abdul Rahman  Denis J. Wright 《Pest management science》1996,48(1):89-97

The efficacy of Bacillus thuringiensis (Bt) subsp. kurstaki HD-1 (‘Dipel’™; Btk; CryIA & CryII) and Bt. subsp. aizawai (‘Florbac’™; Bta; CryIA & CryIC) was assessed against larvae from various field populations of Plutella xylostella (F2 generation) collected in the Cameron Highlands, Malaysia in April 1994 and a lowland population (SERD 2; F10 generation) collected in December 1993. Evidence of resistance to Btk and to a lesser extent Bta is reported in these populations (LC₅₀ Toxicity Ratios [TR]=3–14 and 2–8 respectively), most notably in SERD 2. The first recorded evidence of resistance to abamectin (TR=17–195-fold) in field populations of P. xylostella is also reported. In an unselected sub-population of SERD 2, the TR values for Btk, Bta and abamectin declined 2- to 3-fold (P<0·01) over six generations in the laboratory (F10–F16) while in sub-populations of SERD 2 selected with these products (F11–F15) there was a significant (P<0·01) increase in the TR (15-, 3- and 2·5-fold respectively) when compared with the F10 generation. This suggests the presence of marked resistance to Btk and some resistance to Bta and abamectin. There is also evidence of slight cross-resistance to Btk in the Bta-selected sub-population but no evidence for the reverse selection of resistance or for cross-resistance between Btk and abamectin. Concurrent selection studies (F11–F15) with another sub-population of SERD 2 demonstrated resistance to the acylurea insect growth regulator, teflubenzuron (‘Nomolt’™) (29-fold increase in TR). Based on the selection experiments with SERD 2, estimates of realised heritability (h²) of resistance gave very high values for teflubenzuron and Btk (c.0·7) and moderate values for abamectin and Bta (c.0·3). The results are discussed in relation to integrated pest management (IPM) and insecticide resistance management (IRM) strategies for P. xylostella.  相似文献   

Growth and Physiological Responses of Quinoa to Drought and Temperature Stress          下载免费PDF全文

A. Yang  S. S. Akhtar  M. Amjad  S. Iqbal  S.‐E. Jacobsen 《Journal of Agronomy and Crop Science》2016,202(6):445-453

Quinoa (Chenopodium quinoa Willd.), traditionally called the mother of grains, has the potential to grow under high temperatures and drought, tolerating levels regarded as stresses in other crop species. A pot experiment was conducted in a climate chamber to investigate the potential of quinoa tolerance to increasing drought and temperature. Quinoa plants were subjected to three irrigation and two temperature regimes. At low temperature, the day/night climate chamber temperature was maintained at 18/8 °C and 25/20 °C for high temperature throughout the treatment period. The irrigation treatments were full irrigation (FI), deficit irrigation (DI) and alternate root‐zone drying (ARD). FI plants were irrigated daily to the level of the pot's water‐holding capacity. In DI and ARD, 70 % water of FI was applied either to the whole pot or to one side of the pot alternating, respectively. The results indicated that plant height and shoot dry weight significantly decreased by ARD and DI compared to FI treatment both at low and at high temperatures. However, plants in ARD treatment showed significantly higher plant height and shoot dry weight compared to DI especially at higher temperature, which is linked to increased xylem ion content. Higher quinoa plant growth in ARD was associated with increase in water‐use efficiency (WUE_i) due to higher abscisic acid concentration and higher nutrient contents compared to DI. From results, it can be concluded that quinoa plant growth is favoured by high temperature (25/20 °C) and ARD is an effective irrigation strategy to increase WUE in drought prone areas.  相似文献   

Genetic Variation for Salinity Tolerance in Pakistani Rice (Oryza sativa L.) Germplasm          下载免费PDF全文

A. Sakina  I. Ahmed  A. Shahzad  M. Iqbal  M. Asif 《Journal of Agronomy and Crop Science》2016,202(1):25-36

Soil salinity is one of the major production constraints. Development and planting of salt‐tolerant varieties can reduce yield losses due to salinity. We screened 185 rice genotypes at germination stage in petri dishes under control, 50, 100 and 150 mm salt stress, and at seedling stage in Yoshida's hydroponic nutrient solution under control, 50 and 100 mm salt stress. At germination stage, 15 genotypes including Nona Bokra, Sonahri Kangni, 7421, 7423 and 7467, whereas at seedling stage, 28 genotypes including Nona Bokra, Jajai‐77, KSK‐133, KSK‐282, Fakhr‐e‐Malakand, Pakhal, IR‐6, Khushboo‐95, Shahkar and Shua‐92 were found salt tolerant. Basmati‐370, Mushkan, Homo‐46 and accessions 7436, 7437 and 7720 were sensitive to salinity at both germination and seedling stage. We further screened a subset of 33 salt‐tolerant and salt‐sensitive genotypes with SSR markers. Four SSR markers (RM19, RM171, RM172 and RM189) showed significant association with two or more of the studied traits under 50, 100 and 150 mm salt stress. These markers may be further tested for their potential in marker‐assisted selection. The salt‐tolerant genotypes identified in this study may prove useful in the development of salt‐tolerant rice varieties in adapted genetic background.  相似文献   

Effect of ionic liquid on thermo-physical properties of bamboo biomass     

Nawshad Muhammad  Yanan Gao  Muhammad Irfan Khan  Zakir Khan  Abdur Rahim  Farasat Iqbal  Amir Sada khan  Jibran Iqbal 《Wood Science and Technology》2015,49(5):897-913

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