首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   32998篇
  免费   1016篇
  国内免费   1116篇
林业   4804篇
农学   2541篇
基础科学   756篇
  5108篇
综合类   6270篇
农作物   3270篇
水产渔业   2685篇
畜牧兽医   4828篇
园艺   2067篇
植物保护   2801篇
  2024年   65篇
  2023年   276篇
  2022年   524篇
  2021年   700篇
  2020年   579篇
  2019年   591篇
  2018年   3171篇
  2017年   3335篇
  2016年   1747篇
  2015年   801篇
  2014年   804篇
  2013年   979篇
  2012年   2124篇
  2011年   3536篇
  2010年   3241篇
  2009年   2237篇
  2008年   2419篇
  2007年   2592篇
  2006年   946篇
  2005年   821篇
  2004年   630篇
  2003年   523篇
  2002年   495篇
  2001年   308篇
  2000年   304篇
  1999年   180篇
  1998年   93篇
  1997年   62篇
  1996年   72篇
  1995年   62篇
  1994年   43篇
  1993年   65篇
  1992年   68篇
  1991年   44篇
  1990年   51篇
  1989年   59篇
  1988年   52篇
  1987年   54篇
  1986年   22篇
  1985年   33篇
  1984年   26篇
  1983年   31篇
  1981年   24篇
  1978年   24篇
  1977年   24篇
  1976年   30篇
  1974年   22篇
  1973年   22篇
  1972年   24篇
  1971年   17篇
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
31.
乳酸菌与中药合生元对肉鸡生长性能及饲料利用率的影响   总被引:3,自引:0,他引:3  
本试验分别向肉仔鸡日粮中添加等量的抗生素和乳酸菌与中药合生元制剂,通过测定肉仔鸡的各项生长指标和饲料利用率指标,探讨乳酸菌与中药合生元在肉鸡生长和饲料利用率方面替代抗生素的优势.结果表明,试验组与对照组在生长性能和饲料利用率上各指标均差异不显著(P〉0.05),说明乳酸菌与中药合生元可以替代抗生素饲喂给肉仔鸡.  相似文献   
32.
33.
34.
35.
应用种子蛋白电泳图谱对高羊茅品种进行鉴别与聚类研究   总被引:2,自引:2,他引:0  
利用种子贮藏蛋白进行二二烷其磺酸钠-聚丙烯酰胺凝脉电泳,对23个高羊茅品种进行了 分析。表明该方法成本低,效率高,重复性好,能有效地反应高羊茅品种的遗传差异和亲缘关系。电泳谱带聚类分析所得到的类群,能代表生产上,饲用,草坪用类型以及两种用途中的主要生态类型。  相似文献   
36.
1. Light‐weight (Babcock B300) and medium‐weight (Warren‐SSL) hens were offered diets, containing 0, 100 or 200 g dried poultry manure (DPM) and 11.49, 11.12 or 10.83 MJ metabolisable energy/kg respectively, between 18 and 70 weeks of age. The true protein, metabolisable energy, calcium and phosphorus contents of the DPM, from caged layer hens, were used in the diet formulation.

2. The hen‐housed laying performance of hens offered the DPM‐containing diets was significantly better for number of eggs laid and total egg mass produced but the performance of the control groups was lower than would normally be expected of those stocks. The reason for the lower rate of lay and higher mortality of hens fed on the control diet was not identified. The amount of food consumed increased as the energy content of the diet decreased and the efficiency of food conversion (EFC) of the lightweight strain was reduced; there was no significant effect on the EFC of the medium‐weight hens.

3. The inclusion of DPM in the diets had no effect on albumen quality or on the incidence of hair‐cracked, cracked or broken eggs. However, with both strains of hens, shell weight and shell thickness were poorer when 100 or 200 g DPM/kg diets were fed.  相似文献   

37.
38.
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   
39.
40.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号